{"title":"Immunology \u0026 Inflammation","description":"","products":[{"product_id":"digital-tube-roller-variable-speed-6-rollers-120-230-vac-50-60hz-dlab-mx-t6-pro","title":"MX-T6-Pro Digital Tube Roller - Variable Speed - 6 rollers - 120-230 VAC 50\/60Hz","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo tube rollers provide a gentle and highly efficient rolling motions, ideal for mixing blood samples. viscous substances and liquid-solid suspensions. Used in a variety of applications, such as the prevention of blood coagulation, immune precipitation, etc. \u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications \u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eMX-T6-Pro\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eAmplitude\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e24mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eMotor type\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eDC motor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eRocking motion\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eRocking and rolling\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eAmplitude\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e24mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eMax. load capacity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e4kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eNumber of rollers\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eRoller size[length]\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e280mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eSpeed range\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e10 ~ 70rpm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eSpeed display\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eLCD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eTimer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eTimer display\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eLCD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eTime setting range\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e1~1199min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eOperation mode\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eContinuous\/timer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eVoltage\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e100-240V,50\/60Hz\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003ePower\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e30W\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eDimension [D×W×H]\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e450×260×120mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eWeight\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e5.1kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003ePermissible ambient temperature and humidity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003e5-40°C, 80%RH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eProtection class\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 30px; text-align: center; vertical-align: middle;\"\u003eIP21\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eLCD displays the speed and time\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSpeed range of 10~70rpm\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSix roller design for gentle rolling and easy to clean\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eContinuous or timer operation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"DLAB","offers":[{"title":"MX-T6-Pro","offer_id":52332853330101,"sku":"8063312100","price":622.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MX-T6-Pro.jpg?v=1773039057"},{"product_id":"copy-of-cell-culture-flasks-tc-treated-vented-cap-whb-ccf25-1-ccf75-1-ccf175-1","title":"T25 Cell Culture Flasks with Vented Cap TC Treated Sterile Biofargo","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2\u003cmeta charset=\"UTF-8\"\u003eμm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-cell-culture-flasks-tc-treated-vented-cap-whb-ccf25-1-ccf75-1-ccf175-1?variant=41407698534581\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/whb-ccf75-1?variant=41407679266997\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-t75-cell-culture-flasks-tc-treated-vented-cap-whb-ccf75-1?variant=41407689457845\"\u003eT175\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: PP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 25cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e","brand":"WHB","offers":[{"title":"10Pcs\/Pack","offer_id":41261789642933,"sku":"WHB-CCF25-1","price":12.0,"currency_code":"USD","in_stock":true},{"title":"100Pcs\/Case","offer_id":41407698534581,"sku":"WHB-CCF25-1-CSS","price":117.0,"currency_code":"USD","in_stock":true},{"title":"200Pcs\/Case","offer_id":41261789675701,"sku":"WHB-CCF25-1-CS","price":234.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/CellCultureFlasks1_7b775d38-9fc5-4fe4-a9d3-98963332a7ec.png?v=1732884231"},{"product_id":"copy-of-multiwell-cell-culture-plate-50-cs-tc-treated-flat-bottom-whb-6-12-24-48-97","title":"6 Well PS Cell Culture Plate TC Treated Flat Bottom with Lid Sterile Individual Packed Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHigh-quality TC-treated multiwell cell culture plate made from optically clear polystyrene, designed for reliable adherent cell culture and biological assays. Sterilized, DNase\/RNase-free and non-pyrogenic, these flat bottom plates deliver excellent optical clarity for microscopy and consistent cell attachment across various well formats (6, 12, 24, 48 and 96 wells), and are fully compatible with both manual handling and automated laboratory systems. With a tight yet ventilation-friendly lid and anti-contamination design, they offer reduced evaporation and cross-contamination risk during incubation and experimental workflows. Supported by ANSI footprint standards and ideal for cell biology, drug testing, toxicity assays, and tissue engineering research.\u003c\/p\u003e\n\u003ch2\u003eMaterial and Dimension\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e Polystyrene, Gamma Sterilized\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ABUIABACGAAgk4fi8wUoyPzB3wEwhAI4qgE_480x480.jpg?v=1630599292\" alt=\"\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDimension: \u003c\/strong\u003eD1=35.5mm D2=34.8mm H=17.5mm\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTissue Culture (TC) Treated: Optimized surface for enhanced cell adhesion and growth.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSterile \u0026amp; High Purity: Gamma sterilized, non-pyrogenic, DNase\/RNase-free for dependable cell culture performance.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFlat Bottom Optical Clarity: Clear polystyrene flat wells for distortion-free microscopic observation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAnti-Contamination Design: Raised well rims and condensation rings for minimized evaporation and cross-contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMultiple Well Formats: Available in 6, 12, 24, 48 and 96 well configurations to meet diverse experimental needs.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAutomation \u0026amp; Compatibility: ANSI footprint and stackable design ideal for automated systems and high-throughput workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAdherent cell culture experiments\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCell proliferation and cytotoxicity assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDrug screening and pharmacology studies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStem cell research and differentiation assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMicroscopy and imaging requiring clear well observation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAutomation and high-throughput screening workflows\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"WHB","offers":[{"title":"24Pcs\/Case","offer_id":41407712198837,"sku":"WHB-6-CSS","price":41.0,"currency_code":"USD","in_stock":true},{"title":"50Pcs\/Case","offer_id":41262327791797,"sku":"WHB-6-CS","price":82.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/ABUIABACGAAgoO7h8wUopIbCfTCuBDiuBA_4c6645fb-2a9d-44d4-bbec-f8f7563acb8e.jpg?v=1732865012"},{"product_id":"copy-of-multiwell-cell-culture-plate-50-cs-tc-treated-flat-bottom-whb-6-12-24-48-99","title":"24 Well PS Cell Culture Plate TC Treated Flat Bottom with Lid Sterile Individual Packed Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eOur Multiwell Cell Culture Plates are high-quality tissue culture treated (TC treated) plates designed for robust and reproducible cell culture applications. Made from premium crystal-clear polystyrene, each plate features a flat bottom surface that provides excellent optical clarity for microscopy and imaging. These sterile plates are DNase\/RNase free, non-pyrogenic, and non-cytotoxic, ensuring reliable performance for adherent and suspension cell growth studies. Available in multiple formats including 6, 12, 24, 48, and 96-well, they support a wide range of assays from basic cell maintenance to advanced research workflows. Compatible with automated systems, these plates meet standard ANSI\/SBS footprint dimensions for laboratory automation systems.\u003c\/p\u003e\n\u003ch2\u003eMaterial and Dimension\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eMaterial:\u003c\/strong\u003e Polystyrene, Gamma Sterilized\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ABUIABACGAAgk4fi8wUoyPzB3wEwhAI4qgE_480x480.jpg?v=1630599292\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDimension: \u003c\/strong\u003eD1=16.3mm D2=15.6mm H=18.0mm\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC Treated Surface: Enhanced tissue culture treatment promotes strong cell adhesion for reliable, reproducible results with adherent cell lines.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOptically Clear Flat Bottom: Crystal-clear polystyrene and flat surface ideal for microscopy, imaging, and live cell analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSterile \u0026amp; Biocompatible: Gamma-irradiated, non-pyrogenic, DNase\/RNase free, and non-cytotoxic for clean and safe cell growth.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMulti-Format Options: Available in 6, 12, 24, 48 and 96-well formats to suit different experimental needs.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLaboratory-Friendly Design: Raised well rims reduce evaporation; ANSI\/SBS footprint ensures compatibility with automated systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eThese multiwell tissue culture plates are ideal for:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eCell Culture \u0026amp; Expansion: Adherent and suspension cell growth.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-throughput Screening \u0026amp; Assays: Compatible with plate readers and automated systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMicroscopy \u0026amp; Imaging: Ideal flat bottom for live cell imaging and fluorescence studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDrug Screening \u0026amp; Toxicology: Reproducible surface treatment ensures consistent biological responses.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMolecular Biology \u0026amp; Biotech Research: Plate formats for protein expression, transfection, and cell signaling studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"WHB","offers":[{"title":"24Pcs\/Case","offer_id":41407759417525,"sku":"WHB-24-CSS","price":41.0,"currency_code":"USD","in_stock":true},{"title":"50Pcs\/Case","offer_id":41262339621045,"sku":"WHB-24-CS","price":82.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/ABUIABACGAAgoO7h8wUopIbCfTCuBDiuBA_0e2ea053-39a9-4e17-92c0-a8e34b56a59b.jpg?v=1732864754"},{"product_id":"copy-of-384-well-cell-culture-plate-50-cs-tc-treated-flat-bottom-whb","title":"384 Well PS Cell Culture Plate TC Treated Flat Bottom with Lid Sterile Individual Packed Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo 384‑Well TC Treated Flat Bottom Cell Culture Plate is a premium sterile tissue culture consumable designed for reliable adherent cell growth, high‑throughput assays, and plate‑based biology workflows. Made from crystal‑clear polystyrene with a tissue culture‑treated (TC treated) surface, this 384‑well plate provides uniform cell attachment with superior optical clarity for microscopy and imaging. Each plate features condensation rings, low‑evaporation lids, alphanumeric well ID, and stackable design to enhance contamination control and lab workflow efficiency. Certified gamma sterilized, DNase\/RNase free, and non‑pyrogenic, it is compatible with standard incubators, microscopes, automated liquid handlers, and plate readers — ideal for adherent cell culture, cytotoxicity assays, and molecular biology research.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC Treated Tissue Culture Surface – Enhances adherence and uniform growth of mammalian and other adherent cells.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCrystal‑Clear Flat Bottom Wells – Optimized for high‑quality imaging, microscopy, and optical detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSterile \u0026amp; DNase\/RNase Free – Gamma sterilized and non‑pyrogenic for contamination‑free experimental results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLow‑Evaporation Lids with Condensation Rings – Reduces media loss, minimizes edge effects and cross‑contamination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStackable with Alphanumeric Well Identification – Facilitates easy tracking, handling, and high‑throughput workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBiofargo 384‑Well TC Treated Cell Culture Plates are ideal for:\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdherent cell culture \u0026amp; maintenance – Supports robust cell attachment and growth.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCytotoxicity and cell proliferation assays – For drug screening and viability studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfection, gene expression, and molecular assays – Compatible with downstream analysis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMicroscopy and imaging – Flat bottom design enhances optical clarity for live‑cell and fluorescence imaging.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh‑throughput screening \u0026amp; automation – Standard SBS footprint makes it compatible with automated systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"WHB","offers":[{"title":"50Pcs\/Case","offer_id":41287501414581,"sku":"TC-384","price":218.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/Celltreat_229138_384_Well_Tissue_Culture_Treated_Plate_-_Lab_Supplies_-_Stellar_Scientific__94664.1625495091.jpg?v=1732864887"},{"product_id":"lab-animal-ear-tag-applicator-continuous-smart-tager-clarkbio-st101","title":"First Generation SMART Tager Applicator - Biofargo","description":"\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cp\u003eSingle ear tag inside: 7\/16\" length × 9\/64\" height\u003c\/p\u003e\n\u003cp\u003eSingle ear tag outside: 9\/16\" length × 3\/16\" height × 0.140\" width\u003c\/p\u003e\n\u003ch2\u003eFeature\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSmall animal ear tagging and labeling for research\/lab animals including mice, rats, fish, etc.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe SMART Tager is an innovative applicator designed for continuous and fast application of ear tags to lab animals for purposes of identification, categorization, and management.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUnlike other ear tag applicators, the SMART Tager is specially designed for continuous ear tagging. A single load of the ear tag strip can label up to 10 animals. It’s easy, fast, and efficient!\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe SMART Tager and tags can be sterilized at 135°C for one hour. Tags are made from clinically proven metals, and ear tag strips are bonded with medical-grade epoxy resin, which will not cause allergies or tissue damage.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMaterial: Stainless steel and alloy.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eWork Flow\u003c\/h2\u003e\n\u003cp\u003eSmall animal ear tagging and labeling\u003c\/p\u003e\n\u003cp\u003eResearch\/lab animals including mouse, rat, fish, etc.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/step_480x480.jpg?v=1658503424\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eMatching Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/smart-tager?variant=42295693967541\"\u003eSmart Tager Clips- 5 digit Laser-Etched 1 side - ClarkBio\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp align=\"center\" style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/clark-bioscience-smart-tager-3-digit-laser-etched-1-side?variant=42296430919861\"\u003eSmart Tager Clips- 3 digit Laser-Etched 1 side - ClarkBio \u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2 align=\"center\" style=\"text-align: left;\"\u003eOperate Video\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/videos\/c\/o\/v\/d87c5b8ffe0948a6871b1383014171a5.mp4\"\u003eSmart Tager application to mice\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"ClarkBio","offers":[{"title":"Default Title","offer_id":41322952360117,"sku":"ST101","price":289.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SmartTagerClips_Etched1side-ClarkBio-Biofargo02.jpg?v=1762829822"},{"product_id":"general-advanced-glycation-end-product-age-elisa-kit","title":"General Advanced Glycation End Product (AGE) ELISA Kit","description":"\u003cp\u003eSynonyms: AGEs; Advanced Glycation End Products\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eDetection range: 98.8-8,000ng\/mL\u003c\/p\u003e\n\u003cp\u003eSize: 96T\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eAssay length 1-4.5Hours\u003c\/p\u003e\n\u003cp\u003ePrice: inquiry\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eQuality guarantee period: for 12 months\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eIntroduction\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTest Result\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDescription\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eThis immunoassay kit allows for the specific measurement of this index in serum, Plasma , Urine ,tissue homogenates and Cell culture supernates and\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eOther biological fluids..\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eConform\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIdentification\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eColorimetric\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePositive\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePre-coated, ready to use 96-well strip plate\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard (freeze dried)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStandard Diluent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 20ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDetection Reagent A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1× 120μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eDetection Reagent B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1× 120μl\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAssay Diluent A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 12ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eComposition\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAssay Diluent B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e \u003c\/b\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 12ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eConform\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eTMB Substhumane\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 × 9ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eStop Solution\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 ×6ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eWash Buffer(30 x concenthumane)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1 ×20ml\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePlate sealer for 96 wells\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"169\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"718\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eInstruction manual\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"115\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"87\"\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cbr\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eTest principle\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\nThe microtiter plate provided in this kit has been pre-coated with an antibody specific to this index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for this index and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eRecovery\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMatrices listed below were spiked with certain level of recombinant the index and the recovery humanes were calculated by comparing the measured value to the expected amount of the index in samples.\u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eMatrix\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRecovery range (%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAverage(%)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eserum(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e81-93\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e86\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eEDTA plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-97\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e88\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"433\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eheparin plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"403\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e90-101\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"252\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e95\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eLinearity\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\nThe linearity of the kit was assayed by testing samples spiked with appropriate concenthumanion of the index and their serial dilutions. The results were demonsthumaned by the percentage of calculated concenthumanion to the expected.\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ctable data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eSample\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：2\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：4\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：8\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1：16\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eserum(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e82-96%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e83-98%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e81-99%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e93-101%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eEDTA plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e88-101%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e86-95%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e90-102%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-93%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"396\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eheparin plasma(n=5)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"177\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e80-91%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"156\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e82-90%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"178\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e95-104%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd data-mce-fragment=\"1\" width=\"181\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e79-95%\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003ePrecision\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.\u003c\/p\u003e\n\u003cp\u003eCV(%) = SD\/meanX100\u003c\/p\u003e\n\u003cp\u003eIntra-Assay: CV\u0026lt;10%\u003c\/p\u003e\n\u003cp\u003eInter-Assay: CV\u0026lt;12%\u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\n\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eStability\u003c\/b\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\nThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\u003cbr\u003eTo minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003e\u003cstrong data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eAssay procedure summary\u003c\/b\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e1.Prepare all reagents, samples andstandards;\u003c\/p\u003e\n\u003cp\u003e2.Add 100µL standard or sample to each well. Incubate 2 hours at37oC;\u003c\/p\u003e\n\u003cp\u003e3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at37oC;\u003c\/p\u003e\n\u003cp\u003e4.Aspirate and wash 3times;\u003c\/p\u003e\n\u003cp\u003e5.Add 100µL prepared Detection Reagent B. Incubate 1hour at37oC;\u003c\/p\u003e\n\u003cp\u003e6.Aspirate and wash 5times;\u003c\/p\u003e\n\u003cp\u003e7.Add 90µL Substrate Solution. Incubate 15-25 minutes at37oC;\u003c\/p\u003e\n\u003cp\u003e8.Add 50µL Stop Solution. Read at 450nm\u003c\/p\u003e\n \u003cbr\u003e \n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e","brand":"wytsci","offers":[{"title":"96T","offer_id":52286468587701,"sku":"YB-AGE-Ge","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_30f7416d-9dcb-4a09-8d5b-d1eb2b598893.jpg?v=1753176722"},{"product_id":"fetal-bovine-serum-fbs-volume-500ml-s711-001s","title":"Premium USDA-Origin Fetal Bovine Serum (FBS)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eFetal bovine serum is a highly cost-effective USDA origin serum, from an area free of BSE. The product is suitable for most cell lines and stem cells and has excellent performance in primary cell culture.\u003c\/p\u003e\n\u003cp\u003eFetal bovine serum (FBS) is widely used in eukaryotic cell culture, typically as a supplement for growth media. Fetal bovine serum contains nutritional and embryonic growth promoting factors that are essential for a successful cell culture. Further, fetal bovine serum contains various small molecules, such as, e.g., amino acids, lipids, sugars, and hormones.\u003c\/p\u003e\n\u003cp\u003eFetal bovine serum (FBS) is the blood component obtained after coagulation and removal of cellular components. Its unique and comprehensive mix makes FBS one of the most important supplements for supporting cell growth and proliferation for in vitro cell culture.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCat Number\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eP711S50, P711S500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e500 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eState\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eFrozen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eCell Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eSterility\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eSterile-filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eType\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eCell Growth Media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTriple 0.1 um Sterile filtered\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMycoplasma detection and virus screening\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLegal imports,No freezing and thawing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis treatment allows to destroy the complement acting in the immune system to avoid an interference with some experimentation, in particular the tests of immunology. Excellent for research involving immune response, viral studies, cytotoxicity assays, and stem cells.\u003c\/p\u003e\n\u003ch2\u003e6 Reasons to Choose Our Premium Serum\u003c\/h2\u003e\n\u003cp class=\"\"\u003e\u003cstrong\u003e\u003cspan\u003e#1 - \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eManufactured by Lonsera, our in-house brand, \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003etrusted \u003c\/span\u003e\u003c\/strong\u003e\u003ca href=\"https:\/\/www.serumindustry.org\/\" target=\"_blank\"\u003e\u003cstrong\u003e\u003cspan\u003eISIA Member\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003cstrong\u003e\u003cspan\u003e for 10+ years\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"\"\u003e\u003cstrong\u003e\u003cspan\u003e#2 - \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eCited by hundreds of researchers worldwide (citations available upon request).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"\"\u003e\u003cstrong\u003e\u003cspan\u003e#3 - \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eLocal inventory available in Richmond, VA, with \u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003ea 1-2 day lead time anywhere in the U.S\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"\"\u003e\u003cstrong\u003e\u003cspan\u003e#4\u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e - High lot-to-lot consistency and low endotoxin \u0026lt; 1.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"\"\u003e\u003cstrong\u003e\u003cspan\u003e#5 - \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003eThere’s no risk—you can \u003c\/span\u003e\u003ca href=\"https:\/\/us21.list-manage.com\/survey?u=23c780fa3d44ff41560579881\u0026amp;id=80eab013a1\u0026amp;attribution=false\"\u003e\u003cstrong\u003e\u003cspan\u003eRequest a Free Sample\u003c\/span\u003e\u003c\/strong\u003e\u003c\/a\u003e\u003cspan\u003e at no cost to you!\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"\"\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePlease note: For U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/FBS_CoA.pdf?v=1747934274\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCertificate of Analysis\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/us21.list-manage.com\/survey?u=23c780fa3d44ff41560579881\u0026amp;id=80eab013a1\u0026amp;attribution=false\"\u003e\u003cstrong\u003eRequest Free Sample\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Lonsera","offers":[{"title":"50mL","offer_id":43103313363125,"sku":"P711S50","price":67.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43103313330357,"sku":"P711S500","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/m20230327104850.png?v=1753527137"},{"product_id":"fetal-bovine-serum-volume-500ml-a511-001-from-australia","title":"Fetal bovine serum, Volume 500ml, A511S500, from Australia USDA - Lonsera","description":"\u003cdiv class=\"product-details-module__title ec-header-h6\"\u003e\u003cstrong\u003eProduct Details:\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv class=\"product-details-module__content\"\u003e\n\u003cdiv id=\"productDescription\" class=\"product-details__product-description\"\u003e\n\u003ctable class=\"ecwid-description-table\" style=\"width: 100.093%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eCat Number\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003eA511S500, (formerly A511-001)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003e500 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eState\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003eFrozen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003eCell Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eSterility\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003eSterile-filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 39.8848%;\"\u003eType\u003c\/td\u003e\n\u003ctd style=\"width: 59.9236%;\"\u003eCell Growth Media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eAustralian serum specifically refers to serum from Australia and New Zealand, which is considered a representative of high quality. The place of origin is located in an island country, the animal husbandry industry is well developed, and the disease control is very stable. It is a well-known non-mad cow disease area, and the output of serum is relatively stable. Australian serum is suitable for almost all delicate and difficult cell lines, such as various primary cells, nerve cells, immune cells, and stem cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Triple 0.1 µm sterile-filtered \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Mycoplasma-tested and virus-screened \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e• Ships on dry ice. Store frozen at -20 to -10°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003ePlease note: \u003cmeta charset=\"utf-8\"\u003eFor U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42779749023925,"sku":"A511S500","price":874.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/a512-001.jpg?v=1732872170"},{"product_id":"standard-grade-fetal-bovine-serum-fbs-volume-500ml-s811-001","title":"Standard-Grade: Fetal Bovine Serum (FBS), Volume 50ml \u0026500ml, S811S USDA - Lonsera","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eThis product has super high cost performance, and its physical and chemical indicators can be completely comparable to the top-grade fetal bovine serum. It is an economical and effective product that is widely applicable to the growth and maintenance of various types of cells.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFetal bovine serum is a highly cost-effective serum that is of USDA origin, in an area free of BSE. The product is suitable for most cell lines and stem cells and has excellent performance in primary cell culture.\u003c\/p\u003e\n\u003cp\u003eFetal bovine serum (FBS) is widely used in eukaryotic cell culture, typically as a supplement for growth media. Fetal bovine serum contains nutritional and embryonic growth promoting factors that are essential for a successful cell culture. Further, fetal bovine serum contains various small molecules, such as, e.g., amino acids, lipids, sugars, and hormones.\u003c\/p\u003e\n\u003cp\u003eFetal bovine serum (FBS) is the blood component obtained after coagulation and removal of cellular components. Its unique and comprehensive mix makes FBS one of the most important supplements for supporting cell growth and proliferation for in vitro cell culture.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable height=\"171\" width=\"553\" class=\"ecwid-description-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003e\n\u003cp style=\"text-align: left;\"\u003eCat Number\u003cbr\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003eS811S50, S811S500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003e500 ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eState\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003eFrozen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003eCell Assay\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eSterility\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003eSterile-filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 212.212px;\"\u003eType\u003c\/td\u003e\n\u003ctd style=\"width: 319.788px;\"\u003eCell Growth Media\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTriple 0.1 um Sterile filtered\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMycoplasma detection and virus screening\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLegal imports,No freezing and thawing\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis treatment allows to destroy the complement acting in the immune system to avoid an interference with some experimentation, in particular the tests of immunology. Excellent for research involving immune response, viral studies, cytotoxicity assays, and stem cells.\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003ePlease note: \u003cmeta charset=\"utf-8\"\u003eFor U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\u003c\/p\u003e","brand":"Lonsera","offers":[{"title":"50mL","offer_id":43103314215093,"sku":"S811S50","price":42.0,"currency_code":"USD","in_stock":true},{"title":"500mL","offer_id":43103314182325,"sku":"S811S500","price":371.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/s811-001.jpg?v=1732883582"},{"product_id":"low-igg-fetal-bovine-serum-volume-500ml-u812-001","title":"Low-IgG Fetal Bovine Serum(L812S500)-Volume 500ml USDA - Lonsera","description":"\u003ch2 data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\"\u003e\u003cb\u003eProduct Details\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp data-zone-id=\"0\" data-line-index=\"1\" data-line=\"true\"\u003eOur Low IgG Fetal Bovine Serum is expertly curated from select lots with naturally low levels of Immunoglobulin G (IgG), the major immunoglobulin in normal bovine serum, which makes up 70-75% of the total immunoglobulin pool. Recognized for its critical role in immune function and its unique ability to cross the placenta, IgG in natural bovine serum can sometimes interfere with specific cell culture studies, particularly when the research involves the production of unique IgG molecules.\u003c\/p\u003e\n\u003cp data-zone-id=\"0\" data-line-index=\"3\" data-line=\"true\"\u003eTo maintain the highest integrity and functionality in your cell culture and immunoassay applications, our FBS is processed without artificial methods to remove IgG, which could potentially impair the serum's performance. For applications requiring even lower levels of IgG, we offer batches that have undergone a proprietary chromatography process to further reduce the IgG content to less than 5 µg\/mL.\u003c\/p\u003e\n\u003cdiv data-zone-id=\"0\" data-line-index=\"3\" data-line=\"true\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv data-zone-id=\"0\" data-line-index=\"3\" data-line=\"true\"\u003e\n\u003ch2 data-line=\"true\" data-line-index=\"0\" data-zone-id=\"0\"\u003e\u003cb\u003eFeatures\u003c\/b\u003e\u003c\/h2\u003e\n\u003cul start=\"1\"\u003e\n\u003cli data-list=\"bullet1\" data-line=\"true\" class=\"temp-li bullet1\"\u003e\n\u003cb\u003eUltra-Low IgG Content\u003c\/b\u003e: Our FBS is carefully selected from lots with naturally low IgG levels and further processed through proprietary chromatography to reduce IgG content to below 5 µg\/mL, ideal for sensitive applications.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"1\"\u003e\n\u003cli data-list=\"bullet1\" data-line=\"true\" class=\"temp-li bullet1\"\u003e\n\u003cb\u003eTriple 0.1 µm Sterile Filtration\u003c\/b\u003e: Ensures the highest sterility by passing through stringent triple 0.1 µm filters, removing any potential microbial contamination.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"2\"\u003e\n\u003cli data-list=\"bullet1\" data-line=\"true\" class=\"temp-li bullet1\"\u003e\n\u003cb\u003eComprehensive Safety Testing\u003c\/b\u003e: Each batch is tested rigorously for mycoplasma, viruses (including BVD), endotoxin levels, hemoglobin, and total protein rates, ensuring safe and reliable use in critical research environments.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul start=\"4\"\u003e\n\u003cli data-list=\"bullet1\" data-line=\"true\" class=\"temp-li bullet1\"\u003e\n\u003cb\u003ePreserved Biological Activity\u003c\/b\u003e: Despite IgG depletion, our serum retains full biological activity, supporting excellent cell growth properties and performance comparable to regular FBS.\u003c\/li\u003e\n\u003cli data-list=\"bullet1\" data-line=\"true\" class=\"temp-li bullet1\"\u003e\n\u003cb\u003eOptimal Storage Conditions\u003c\/b\u003e: Ships on dry ice and should be stored frozen at temperatures ranging from -20 to -10°C to maintain serum integrity and performance.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 data-line=\"true\" data-line-index=\"0\" data-zone-id=\"0\"\u003eResearch areas using ultra-low IgG FBS\u003c\/h2\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"2\" data-zone-id=\"0\"\u003eAntibodies\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"3\" data-zone-id=\"0\"\u003eViruses and viral response\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"4\" data-zone-id=\"0\"\u003eCell-surface epitopes\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"5\" data-zone-id=\"0\"\u003eIgG-dependent cell cytotoxicity\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"6\" data-zone-id=\"0\"\u003eLigand-receptor studies\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"6\" data-zone-id=\"0\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv data-line=\"true\" data-line-index=\"6\" data-zone-id=\"0\"\u003e\n\u003cmeta charset=\"utf-8\"\u003e \u003cstrong\u003ePlease note: \u003cmeta charset=\"utf-8\"\u003eFor U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42780058288309,"sku":"L812S500","price":876.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/u812-001.jpg?v=1732876236"},{"product_id":"dialyzed-fetal-bovine-serum-volume-500ml-u612-001","title":"Dialyzed Fetal Bovine Serum, Volume 500ml, D612S500 USDA - Lonsera","description":"\u003cp\u003e\u003cstrong\u003eProduct Details:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMost cell growth in culture requires a serum component of the growth medium to maintain their proliferative capacity. While whole serum is suitable for routine purposes, studies involving nutritional parameters or the incorporation of labeling materials require elements to be removed from the serum during the study. Typically the whole serum is dialyzed using fractionation methods. For complete filtration dialysis, serum is passed around a hollow fiber by a concentrated method. Dialysis of fetal bovine serum is mainly to remove nucleosides, amino acids, hormones, ions, etc.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eDialysis removes many small molecules from FBS such as glucose, salts and some non-protein bound serum molecules. This process generally does not remove hormones that are serum bound but it may reduce growth promotion capabilities for some cell types. The dialysis process is done by ultra filtration with a 10,000 molecular weight cut-off membrane. The serum is processed at 4°C with a careful monitoring of pH, osmolality and glucose concentration. Sterile-filtered. Mycoplasma-tested and virus-screened. Ships on dry ice. Store frozen at -20 to -10°C\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003cmeta charset=\"utf-8\"\u003e\n\u003cstrong\u003ePlease note: \u003cmeta charset=\"utf-8\"\u003eFor U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42780091351221,"sku":"D612S500","price":842.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/u612-001_1.jpg?v=1753432741"},{"product_id":"newborn-calf-serum-volume-500ml-ua513-001","title":"Newborn Calf Serum, Volume 500ml, C513S500 USDA - Lonsera","description":"\u003cp\u003e\u003cstrong\u003eProduct Details:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eFor calves born within 24 hours, blood was collected from the carotid artery and collected after centrifugation. In order to ensure the quality of serum, newborn cows do not drink colostrum, and the entire collection process is kept aseptic.\u003cbr\u003eCompared with fetal bovine serum, newborn bovine serum has a larger output and a lower price. It is suitable for the cultivation of most cell lines and has a high cost performance. Cell culture can be optimized according to customer requirements.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eFeatures:\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e-Meet industrial customers and scientific research users\u003cbr\u003e-Applicable to various cell lines\u003cbr\u003e-Affordable price, reduce experiment cost\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003e\u003cstrong\u003ePlease note: \u003cmeta charset=\"utf-8\"\u003eFor U.S. Customers, all serum is of USDA origin.\u003c\/strong\u003e\u003c\/p\u003e","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42780181987509,"sku":"C513S500","price":93.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8a42271b-8587-4231-acd3-f787d5d4de87_33f762a8-ca81-4928-93d6-37bc1549cf17.jpg?v=1737444928"},{"product_id":"fast-cell-freeze-no-serum","title":"Rapid Cell cryopreservation solution (no serum)","description":"This product is transported at room temperature; Stored at 2~8℃, shelf life is 12 months; Stored at -20℃, shelf life is 36 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProduct characteristics\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. no serum, clear chemical composition.\u003cbr\u003e\u003cbr\u003e2. Without cooling procedures, it can be directly stored in the -80℃ refrigerator.\u003cbr\u003e\u003cbr\u003e3. Cell recovery rate was above 90%.\u003cbr\u003e\u003cbr\u003e4. 2~8℃ can be stably stored, without freezing, ready to use.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCell resuscitation procedure\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eThe frozen cells were removed and immediately thawed in a 37℃ water bath.\u003cbr\u003eAfter the cell mixture in the cryo-storage tube was completely melted, the cells were immediately transferred into a centrifuge tube containing 5 mL cell medium, and the cells were collected by centrifugation (refer to centrifugation conditions: 800~1,000 rpm, 5 min).\u003cbr\u003eCarefully remove the supernatant. Add appropriate amount of fresh cell medium, mix gently, and transfer the cell mixture to the culture container marked;\u003cbr\u003eAfter microscopic examination, routine cell culture can be carried out according to specific research needs and methods.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eCell cooling procedure (For your safety and health, please wear lab coat and disposable gloves.)\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eAdherent cells or suspended cells in logarithmic growth stage were collected in centrifuge tubes and counted.\u003cbr\u003eCell suspension was placed in a centrifuge tube and cells were collected by centrifugation (refer to centrifugation conditions: 800~1,000 rpm, 5 min). Thoroughly remove the supernatant in the centrifuge tube;\u003cbr\u003eAppropriate amount of cell freeze solution was added into the centrifuge tube and cell concentration was adjusted to 5×105~5×106 cells\/mL. Gently mix to make cell frozen suspension;\u003cbr\u003eThe cell cryopreservation suspension in the centrifuge tube was divided into labeled cryopreservation tubes;\u003cbr\u003eThe cryopreservation tube containing cell suspension was directly placed in the -80℃ refrigerator for long-term cryopreservation.\u003cbr\u003eIf liquid nitrogen is needed for storage, it should be placed in the -80℃ refrigerator overnight and then transferred to the liquid nitrogen tank.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eVerified cell\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eCells verified so far: HEP3B, SNU-387, SNU-423, HLE, JHH-7, A375, A2058, A431, HEK-293, Hela, A549, NCI-H460, CHO, MCF-7, HepG2, C2C12, COS7, DU145, MDCK, NIH-3T3, MSC, PC12, RAW264.7, 769-P, HK-2, T24, M14, U87, U251, 293FT, Jurkat, Raji, K562, HCCLM3, Vero, MC3T3-E1, HCT116, H9C2, SiHa, SK-OV-3, etc.","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42793830088885,"sku":"LS368-001","price":299.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/kuaisuxibaodongcun.png?v=1732881101"},{"product_id":"dm0636-3-10ml-10-15ml-50ml-100ml-basket-with-adaptor-swing-out-rotor-with-four-baskets-with-adaptor","title":"DM0636 Multi-Purpose Clinical Centrifuge with Swing-out Rotor Kit 19400041 \u0026 100ml Basket 19400042","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe DM0636 Multi-Purpose Clinical Centrifuge is a versatile laboratory centrifuge designed for routine clinical diagnostics, biological research, and sample preparation applications. With a speed range of 300–6000 rpm and a maximum relative centrifugal force of 4100 × g, the centrifuge delivers reliable performance for the separation of blood, urine, and other biological samples.\u003c\/p\u003e\n\u003cp\u003eThis model is supplied with a swing-out rotor kit (19400041) and a 100 mL basket (19400042), providing a maximum rotor capacity of 100 mL × 4. The swing-out rotor design ensures uniform sample separation and is particularly suitable for blood collection tubes and other capped sample tubes commonly used in clinical laboratories.\u003c\/p\u003e\n\u003cp\u003eThe centrifuge is powered by a maintenance-free brushless DC motor that provides stable operation, precise speed control, and reduced mechanical wear. The LCD display allows convenient monitoring of speed and run time, while adjustable acceleration and braking profiles (9 acceleration and 10 braking settings) ensure optimal separation conditions for different sample types.\u003c\/p\u003e\n\u003cp\u003eFor enhanced safety and reliability, the DM0636 features door imbalance protection, over-speed detection, and over-temperature monitoring. Automatic rotor identification further improves operational safety and simplifies setup. The system also allows up to nine user-defined programs to be stored in memory, enabling quick recall of frequently used centrifugation protocols.\u003c\/p\u003e\n\u003cp\u003eWith a maximum capacity of 100 mL × 4, quiet operation (≤65 dB), and compatibility with a wide range of rotors and accessories, the DM0636 Multi-Purpose Clinical Centrifuge provides an efficient and flexible centrifugation solution for clinical laboratories, diagnostic facilities, and research institutions.\u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCat.No.\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e9034001122\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSpeed Range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e300–6000 rpm, increment: 10 rpm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMax. RCF\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e4100 × g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSpeed Accuracy\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e±20 rpm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRotor Type\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRotor Capacity\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 mL × 4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRun Time\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e30 sec – 99 min \/ Continuous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDisplay\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eLCD\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSafety Devices\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDoor imbalance protection; over-speed \u0026amp; over-temperature detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAcceleration \/ Braking Time [Sec]\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e9 ↑ \/ 10 ↓ steps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProgram\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003ePreloaded Program\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e-\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProgram Memory\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMotor\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBrushless DC motor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003ePower\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e400 W; Single-phase AC 220–240 V, 50\/60 Hz, 6.3 A; AC 110–120 V, 50\/60 Hz, 10 A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDimension [W × D × H]\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e570 × 445 × 295 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eWeight\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e36 kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eNoise\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e≤65 dB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSpeed from 300–6000 rpm\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMaximum capacity: 100 mL × 4\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e9 acceleration \/ 10 brake speed settings to ensure optimal separation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eA wide range of rotors optional (fixed angle and swing-out rotors)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAutomatic rotor identification\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePulse key for quick spin\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e9 procedures stored in memory makes the operation much simplified\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMaintenance-free brushless DC motor\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSuitable for tubes with caps, blood collection or urine tubes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eComprehensive and versatile range of accessories\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eClinical Blood Separation:\u003c\/strong\u003e Suitable for serum and plasma separation from blood collection tubes in clinical laboratories and diagnostic facilities.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eUrine and Biological Fluid Analysis:\u003c\/strong\u003e Used for centrifugation of urine samples and other clinical specimens prior to microscopic or biochemical analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSample Preparation in Clinical Diagnostics:\u003c\/strong\u003e Supports routine centrifugation steps in medical testing workflows including immunoassays and biochemical testing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell and Particle Sedimentation:\u003c\/strong\u003e Efficient separation of cells, cellular debris, and particulate matter from liquid samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eResearch Laboratory Applications:\u003c\/strong\u003e Suitable for general centrifugation tasks in life science laboratories including microbiology, molecular biology, and biochemical research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eClinical Sample Processing:\u003c\/strong\u003e Compatible with capped tubes, blood collection tubes, and other common laboratory sample containers.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCompoments\u003c\/h2\u003e\n\u003cp\u003eMulti-Purpose Clinical Centrifuge (standard package), with swing-out rotor kit19400041 \u0026amp; 100ml basket 19400042\u003c\/p\u003e","brand":"DLAB","offers":[{"title":"DM0636","offer_id":42888624636085,"sku":"9015001123","price":2608.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DM0636_ecd9b548-2ba7-4cd0-8ab0-26b50aa8a951.jpg?v=1773033438"},{"product_id":"thermophilic-reverse-transcriptase-cat-rt-pcr-140","title":"Thermophilic Reverse Transcriptase-Genetic Tools","description":"\u003ch2\u003e\u003cstrong\u003eIntended use\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis reverse transcriptase or RNA-dependent DNA polymerase is used for real-time fluorescent RT-PCR with either SYBR Green dye or \u003c\/span\u003e\u003cspan\u003eTaqMan probe\u003c\/span\u003e\u003cspan\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Characteristics\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe reverse transcriptase is a thermophilic type A polymerase \u003cspan\u003e(US patent pending) with optimal temperatures at 60-62°C (User Manual Tables 1-2).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe RTase\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eis easily heat-inactivated at ≥90°C for 1min.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe enzyme efficiently\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003esynthesizes a complementary DNA strand on RNA template from a gene-specific primer, ≤\u003cem\u003e1 unit per 20μL of singleplex reaction.\u003c\/em\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eThe enzyme reversely-transcribes single digit copies of target RNA molecules consistently.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe concentrations of the primers are variable depending on assay designs and thermocycling protocols (User Manual Table 4)\u003c\/span\u003e.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003e\u003cspan\u003eThe preferable PCR product size is ≤150bp.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp class=\"\"\u003eIt is particularly suitable to one-step\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003ereal-time qualitative and quantitative RT-PCR.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/140-UM_RTase_combined_07282023.pdf?v=1704698048\" type=\"application\/pdf\" target=\"_blank\"\u003eUser Manual\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"200U \/ Taq-Standard-SYBR-Green","offer_id":42917497995445,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"200U \/ Taq-Fast-SYBR-Green","offer_id":42918688784565,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"200U \/ Taq-Probe","offer_id":42918688817333,"sku":"140-200","price":278.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Standard-SYBR-Green","offer_id":42917498093749,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Fast-SYBR-Green","offer_id":42918688981173,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"500U \/ Taq-Probe","offer_id":42918689013941,"sku":"140-500","price":693.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Standard-SYBR-Green","offer_id":42917498192053,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Fast-SYBR-Green","offer_id":42918689177781,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true},{"title":"2500U \/ Taq-Probe","offer_id":42918689210549,"sku":"140-2500","price":3256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/140-2500U-B.jpg?v=1732884633"},{"product_id":"actsep®-cd3-cd28-separation-activation-magnetic-beads","title":"GMP Grade ActSep® Human TCell CD3\/CD28 Activation \u0026 Expension  Magnetic Beads","description":"\u003cstyle\u003e\n  \/* =========================\n     Base: match CD3 template logic + mobile table scroll\n  ========================== *\/\n\n  .biofargo-table-wrapper{\n    overflow-x: auto;\n    -webkit-overflow-scrolling: touch;\n    margin: 20px 0;\n  }\n\n  \/* Desktop: normal full width *\/\n  .biofargo-spec-table{\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #dee2e6;\n    background: #fff;\n    font-size: 14px;\n    min-width: 600px;\n  }\n\n  .biofargo-spec-table th,\n  .biofargo-spec-table td{\n    border: 1px solid #dee2e6;\n    padding: 12px;\n    text-align: center;\n    vertical-align: middle;\n    white-space: normal;\n    word-break: break-word;\n  }\n\n  \/* Mobile: force horizontal scroll *\/\n  @media (max-width: 600px){\n    .biofargo-table-wrapper{\n      border: 1px solid #eee;\n      border-radius: 8px;\n      padding: 6px;\n      background: #fff;\n    }\n\n    .biofargo-spec-table{\n      width: max-content !important;\n      min-width: 720px !important;\n      display: inline-table !important;\n    }\n\n    .biofargo-spec-table th,\n    .biofargo-spec-table td{\n      white-space: nowrap;\n    }\n  }\n\n  \/* Applications grid (same idea as CD3) *\/\n  .biofargo-app-grid{\n    display: grid;\n    grid-template-columns: 1fr 1fr;\n    gap: 20px;\n  }\n  @media (max-width: 640px){\n    .biofargo-app-grid{\n      grid-template-columns: 1fr;\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\n\u003cp\u003eActSep® CD3\/CD28 Separation \u0026amp; Activation Magnetic Beads are suitable for separation of human CD3+ T cells, providing a simple method for activating and expanding human T cells without the need for antigen presenting cells and antigens.\u003c\/p\u003e\n\u003cp\u003eBy coupling anti-human CD3 and anti-human CD28 antibodies on magnetic beads, the primary and co-stimulatory signals needed to regulate T cell activation and expansion are provided.\u003c\/p\u003e\n\u003cp\u003eThe beads are suitable for human T cell separation, activation and expansion, CAR-T, and other T cell culture applications.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n\u003cth style=\"width: 35%; padding: 12px; text-align: center;\"\u003eTest Item\u003c\/th\u003e\n\u003cth style=\"width: 65%; padding: 12px; text-align: center;\"\u003eSpecification\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eConsistence\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e2 × 10\u003csup\u003e8\u003c\/sup\u003e beads\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eContent\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e1 mL \/ 5 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e\u0026lt; 0.5 EU\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eReactive Species\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eStorage Temperature\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e2–8 °C (Do not freeze)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; font-weight: bold; border: 1px solid #dee2e6; background-color: #f8f9fa; text-align: center;\"\u003eValidity Period\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #dee2e6; text-align: center;\"\u003e24 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeature\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e4.5um size particles\u003c\/strong\u003e\u003cbr\u003eUniform 4.5 μm magnetic particles provide consistent magnetic response and reproducible cell separation performance.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible specifications\u003c\/strong\u003e\u003cbr\u003eFlexible specifications support both small-scale research and scalable cell culture workflows.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh stability and safety\u003c\/strong\u003e\u003cbr\u003eHigh suspension stability and low nonspecific binding ensure safe and reliable operation during cell handling and culture.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStrict QC testinq.traceability documentation\u003c\/strong\u003e\u003cbr\u003eEach batch undergoes strict quality control testing with complete traceability documentation.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eISO9001 and ISO13485 certified\u003c\/strong\u003e\u003cbr\u003eManufactured under ISO 9001 and ISO 13485 certified quality management systems.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProduction in cass B+A clean area\u003c\/strong\u003e\u003cbr\u003eProduced in Class B + A cleanroom environments to ensure controlled manufacturing conditions and product consistency.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplication\u003c\/h2\u003e\n\u003cdiv style=\"margin-top: 12px;\" class=\"biofargo-app-grid\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell activation\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eRapid activation of human CD3⁺ T cells by delivering primary and co-stimulatory signals for efficient initiation of T cell responses.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eImmunotherapy\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eSuitable for immunotherapy research and development, supporting standardized T cell preparation in cell-based immune platforms.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell sorting\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eMagnetic separation and enrichment of human CD3⁺ T cells from PBMCs, providing a high-purity starting population for downstream workflows.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px; background-color: #ffffff;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eT cell seperetion\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eEfficient isolation of CD3⁺ T cells from heterogeneous cell populations to enable controlled activation, expansion, and functional studies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocument\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ActSep_CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1697702677\" target=\"_blank\"\u003e\u003c\/a\u003e\u003c\/strong\u003e\n\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; background-color: #0056b3; color: white; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603 Data Sheet\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-CD3CD28_Separation_Activation_Magnetic_Beads.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603 SDS\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-BD2412005-GMP-TL603-1000.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603-1000 COA\u003c\/a\u003e\n  \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-BD2412009-GMP-TL603-5000.pdf?v=1772521891\" target=\"_blank\"\u003e📄 GMP-TL603-5000 COA\u003c\/a\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eAttention\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5;  padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp\u003e\u003cstrong\u003eFor research use and GMP manufacturing workflows only. Not for direct clinical administration.\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"1mL","offer_id":43164731179189,"sku":"GMP-TL603-1000","price":490.0,"currency_code":"USD","in_stock":true},{"title":"5mL","offer_id":43164731211957,"sku":"GMP-TL603-5000","price":2380.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/GMP-TL603-5000.png?v=1753150142"},{"product_id":"ecovac-economical-vacuum-aspirator","title":"EcoVAC Economical Vacuum Aspirator 2L","description":"\u003ch2\u003eDescription\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp class=\"Polaris-Text--root Polaris-Text--bodyMd Polaris-Text--medium Polaris-Text--breakAlways\" tabindex=\"-1\"\u003eVacpette Economical Vacuum Aspirator is suitable for any application where the single liquid suction volume is less than 2L. The applicability includes use of various common consumables, which can be widely used in the collection and removal of liquids in experiments of microorganisms, molecules, proteins, cells, etc., and other applications such as RNA\/DNA extraction,Western blots and ELISA.\u003c\/p\u003e\n\u003ch2\u003e\u003ca\u003eSpecification\u003c\/a\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 144px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eVacuum range\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e0-500mbar\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eAspirating speed\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e15L\/min(air)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eFlow rate [liquid]\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e17mL\/s\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eBottle\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e2L standard, 1L optional with adaptor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eMotor type\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003eBrushless Motor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eInput voltage\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e100-220VAC, 50\/60Hz\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eDimension [W×D×H]\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e180×240×340 mm\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 180.323px; height: 18px; text-align: center;\"\u003eBottle Volume\u003c\/td\u003e\n\u003ctd style=\"width: 362.344px; height: 18px; text-align: center;\"\u003e2.8kg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSmall and compact to fit in the cabinet, on the benchtop or even beneath the bench\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEasy to switch between manual aspiration and continuous aspiration mode, reducing RSI (Repetitive Strain Injury) and improving efficiency\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCollection bottle outlet with hydrophobic filter prevents aerosol and liquid contamination\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBrushless motor offers low noise and long life\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDifferent adapters are available to fit a multitude of laboratory supplies\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe parts in contact with liquids can be autoclaved\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp style=\"font-size: 0.875rem; display: inline !important;\"\u003eAdjustable vacuum level with indicator light is designed for various applications\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003ca\u003eApplications\u003c\/a\u003e\u003c\/h2\u003e\n\u003cp\u003eEcoVac is suitable for any application where the single liquid suction volume is less than 2L.The applicability includes use of various common consumables,which can be widely used in the collection and removal of liquids in experiments of microorganisms,molecules,proteins,cells,etc.,and other applications such as RNA\/DNA extraction,Western blots and ELISA.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eAspiration of supernatants after centrifugation\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAspiration of eluent\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemoval of supernatants during nucleic acid extraction\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemoval of culture media\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemoval of wash solutions from immuno-assay well-plate\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemoval of buffers of Western blot strips\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRemoval of excess liquid from slides and Petri dishes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2023-10-11_15-21-55_480x480.png?v=1697008953\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct documentation\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/SafeVAC_EcoVac_flyer_1.pdf?v=1696375919\" type=\"application\/pdf\" target=\"_blank\"\u003eSafeVAC \u0026amp; EcoVac flyer\u003c\/a\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOperate Video：\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/www.youtube.com\/watch?v=yYh9wyZQPlc\u0026amp;t=7s\"\u003eoperate video\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"highlighter--hover-tools\" style=\"display: none;\"\u003e\n\u003cdiv id=\"highlighter--hover-tools--container\"\u003e\n\u003cdiv class=\"highlighter--icon highlighter--icon-copy\" title=\"Copy\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"highlighter--icon highlighter--icon-change-color\" title=\"Change Color\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"highlighter--icon highlighter--icon-delete\" title=\"Delete\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"DLAB","offers":[{"title":"EcoVAC","offer_id":52335181037749,"sku":"7035200001","price":533.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EcoVac.webp?v=1732871143"},{"product_id":"human-ifn-γ-precoated-elispot-kitstrips","title":"Human IFN-γ Precoated ELISPOT Kit(strips)-96T-Biosci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThis kit is a ready-to-use kit, including detection antibody, Streptavidin-HRP, AEC coloring system, dilution buffer, cell stimulant, washing buffer, pre-coating PVDF plate, which can meet the needs of ELISPOT experiments. This kit is only used for scientific research. It shall not be used for diagnosis. Read the instruction and check the components of the kit before use.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMaterials Provided\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 198px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 198px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eItem\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eQuantity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eBiotinylated Antibody\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e100 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eStreptavidin-HRP\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e100 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eCell Stimulant-PHA\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eDilution Buffer R(1×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e10 mL×2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eWashing Buffer (50×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e15 mL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Dilution\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e10 mL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅰ (20×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅱ (20×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅲ (200×)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e50 μL×1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003ePrecoated Strip PVDF Plate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eSelf-provided Items\u003c\/h2\u003e\n\u003cp\u003e1. Cell culture medium (It is recommended to use serum-free medium or RPMI-1640 with 100 units\/mL Penicillin,100 μg\/mL Streptomycin and 5-10% fetal calf serum)\u003cbr\u003e2. Lymphocyte Separation Medium\u003cbr\u003e3. Clean bench\u003cbr\u003e4. CO 2 -incubator\u003cbr\u003e5. Multi-channel Pipette, pipette tips\u003cbr\u003e6. Amicroscope or an immune spot image analyzer for spot counting\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation of Reagents\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eWashing Buffer (50×): Diluted with distilled water to prepare 1×Washing buffer at the ratio of 1:50 for standby.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eCell Stimulant-PHA: Sterile 1×PBS is added to resuspend lyophilized powder according to the label information on the vial.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eBiotinylated Antibody: Diluted with Dilution Buffer R (1×) (1:100).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eStreptavidin-HRP: Diluted with Dilution Buffer R (1×) (1:100).\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAEC Coloring System: By referring to the following table, AEC Dilution , AEC Solution Ⅰ (20×), AEC Solution Ⅱ (20×), AEC Solution Ⅲ (200×) are mixed well in a clean container at the ratio of 180:10:10:1. The half-life of the AEC coloring solution is about 30 minutes, and the solution is prepared prior to use.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 104.684%; height: 144px;\" data-mce-style=\"border-collapse: collapse; width: 104.684%; height: 144px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e\n\u003cp\u003eTotal volume\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003eAEC Dilution\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅰ(20×)\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅱ(20×)\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003eAEC Solution Ⅲ(200×)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e0.9 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e2 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e1.8 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e10 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e3 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e2.7 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e150 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e150 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e15 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e4 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e3.6 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e200 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e20 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e4.5 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e250 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e25 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e8 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e7.2 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e400 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e40 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\" data-mce-style=\"width: 20%; height: 18px; text-align: center;\"\u003e10 mL\u003c\/td\u003e\n\u003ctd style=\"width: 15%; height: 18px; text-align: center;\" data-mce-style=\"width: 15%; height: 18px; text-align: center;\"\u003e9 mL\u003c\/td\u003e\n\u003ctd style=\"width: 26%; height: 18px; text-align: center;\" data-mce-style=\"width: 26%; height: 18px; text-align: center;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 17.7023%; height: 18px; text-align: center;\" data-mce-style=\"width: 17.7023%; height: 18px; text-align: center;\"\u003e500 μL\u003c\/td\u003e\n\u003ctd style=\"width: 18.2977%; height: 18px; text-align: center;\" data-mce-style=\"width: 18.2977%; height: 18px; text-align: center;\"\u003e50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperation Process\u003c\/h2\u003e\n\u003ch3\u003eDay 1: Cell activation ( aseptic operation is required)\u003c\/h3\u003e\n\u003cp\u003e1. Pre-coating plate: The PVDF membrane is wetted by adding 200 μL of culture medium to the pre-coating plate, and then it shall be pulled out after standing at room temperature for 5-10 minutes.\u003cbr\u003e2. The 100 μL of cell suspension is added to each well. And the cell concentration is adjusted according to different mitogen or antigen(e.g.Using PHA to stimulate cells, the cell concentration can be 1×10 5 cells\/well,for reference\u003cbr\u003eonly). Only 100 μL of medium is added to the negative control well.\u003cbr\u003e3. The 10 μL of appropriate concentration of antigen or cell stimulant is added to each well.\u003cbr\u003e4. Incubation: After all the samples and stimulants are added, the plate is covered with lid. And the incubation is performed at an incubator with 37°C, 5% CO 2 for 16-24 hours. The specific incubation time varies due to different cell types and cytokines.\u003c\/p\u003e\n\u003ch3\u003eDay 2: Operation after incubation (aseptic operation is no longer required)\u003c\/h3\u003e\n\u003cp\u003e5. The cells and medium in the wells are shaken off; 200 μL of ice-cold deionized water is added to the wells, and placed in a refrigerator at 4°C for 10 minutes to remove the cells through low permeability and pyrolysis.\u003cbr\u003e6. The liquid in the well is shaken off; 260 μL of 1×Washing Buffer is added to each well. The well is washed 6 times, each time for 60 seconds. The plate strips are dried by turning it over and tapping it on the absorbent paper.\u003cbr\u003e7. The 100 μL of diluted Biotinylated Antibody is added to each well; the plate is covered with lid, and the incubation\u003cbr\u003eis performed at 37°C for 1 hour.\u003cbr\u003e8. Repeat Step 6.\u003cbr\u003e9. The 100 μL of diluted Streptavidin-HRP is added to each well; the plate is covered with lid, and the incubation is performed at 37°C for 1 hour.\u003cbr\u003e10. Repeat Step 6.\u003cbr\u003e11. The 100 μLof freshly prepared AEC Coloring solution is added to each well. Stand at a dark place and at room temperature for 5-30 minutes. Note: The spot shape is checked every 5-10 minutes until the spot is clear.\u003cbr\u003e12. The liquid in the well is poured; the base of the plate is uncovered, and the front and back of each experimental well and the base are washed with a large amount of distilled water. Excess water is shaken off; the plate is placed in a cool place and at room temperature.Afterwards, the base is installed after the plate is naturally dried.\u003cbr\u003e13. The spots are counted manually under microscope or by automatic ELISPOT analyzer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eWarnings \u0026amp; Precautions\u003c\/h2\u003e\n\u003ch3 style=\"text-align: left;\" data-mce-style=\"text-align: center;\"\u003eWarnings\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eThis kit is only used for scientific researches. It shall not be used for diagnosis and treatment.\u003c\/li\u003e\n\u003cli\u003eDo not use kits that are beyond the expiration date or with damaged packaging.\u003c\/li\u003e\n\u003cli\u003eDo not use this kit in conjunction with the components of other kits.\u003c\/li\u003e\n\u003cli\u003eWear gloves and avoid direct contact with the skin, when using chemical reagents or biological products, especially using AEC substrates containing dimethylformamide (DMF) which is harmful to the skin and eyes. Once they are stuck to your skin, please immediately rinse with plenty of water and seek medical attention in time.\u003c\/li\u003e\n\u003cli\u003ePlease dispose of unused reagents or biological products according to local laws and regulations.\u003c\/li\u003e\n\u003cli\u003eNote: Pipetting, washing the plate, incubation time and temperature, cell conditions and other factors are related to the formation of spots, the degree of color development and the changes in the background.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003ch3 style=\"text-align: left;\"\u003ePrecautions\u003c\/h3\u003e\n\u003col\u003e\n\u003cli\u003eDo not touch the membrane directly with pipette tips, so as not to scratch or damage the membrane.\u003c\/li\u003e\n\u003cli\u003eAfter completion of the experiment, do not dry the plate at temperature higher than 37ºC, otherwise the membrane will be broken.\u003c\/li\u003e\n\u003cli\u003eBefore terminating the reaction, lift the base baffle and flush the base liquid to prevent the membrane background from aggravating, but please operate gently to avoid direct damage to the membrane.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eThe kit is stored at 2-8ºC and can be stored stably for 12 months. The kit cannot be used after expiration, and remains sterile after opening.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct documentation\u003c\/h2\u003e\n\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/2110005_Human_IFN-_Precoated_ELISPOT_kit_strips_--C0_d841e402-abe0-4a82-82ba-1fc756506cb4.pdf?v=1697160995\" type=\"application\/pdf\" target=\"_blank\"\u003eHuman IFN-γ Precoated ELISPOT kit（strips）datasheet\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e","brand":"Dakewe","offers":[{"title":"96T","offer_id":43170192785589,"sku":"2110005","price":443.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_8b2e6086-5d61-47e4-a462-33632579fbe8.jpg?v=1753176121"},{"product_id":"recombinant-human-epo-protein","title":"Recombinant Human EPO Protein","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Product Page Base\n  ========================== *\/\n  .biofargo-wrap{\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color:#333;\n    line-height:1.65;\n    max-width:1000px;\n    margin:0 auto;\n    background:#fff;\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:28px;\n  }\n  .biofargo-h2{\n    color:#0056b3;\n    border-bottom:2px solid #0056b3;\n    padding-bottom:8px;\n    margin:0 0 14px 0;\n    font-size:22px;\n  }\n  .biofargo-section{ margin-top:28px; }\n  .biofargo-card{\n    background:#fcfcfc;\n    border:1px solid #f0f0f0;\n    border-radius:10px;\n    padding:16px;\n  }\n\n  \/* =========================\n      Table Responsive\n  ========================== *\/\n  .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n  }\n  table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:900px; \/* allow horizontal scroll on mobile *\/\n    background:#fff;\n  }\n  table.biofargo-table th,\n  table.biofargo-table td{\n    border:1px solid #ddd;\n    padding:12px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  table.biofargo-table thead th{\n    background:#0056b3;\n    color:#fff;\n    font-weight:700;\n    text-align:left;\n  }\n  table.biofargo-table thead th.center,\n  table.biofargo-table tbody td.center{\n    text-align:center;\n  }\n  table.biofargo-table tbody td:first-child{\n    font-weight:700;\n    background:#f8f9fa;\n  }\n\n  \/* =========================\n      Features (✔ list)\n  ========================== *\/\n  .biofargo-checklist{\n    list-style:none;\n    padding:0;\n    margin:0;\n  }\n  .biofargo-checklist li{\n    position:relative;\n    padding-left:28px;\n    margin:0 0 14px 0;\n  }\n  .biofargo-checklist li:last-child{ margin-bottom:0; }\n  .biofargo-check{\n    position:absolute;\n    left:0;\n    top:0;\n    color:#0056b3;\n    font-weight:800;\n  }\n  .biofargo-checklist strong{\n    display:block;\n    font-size:15px;\n    margin-bottom:4px;\n  }\n  .biofargo-checklist p{\n    margin:0;\n    font-size:14px;\n  }\n\n  \/* =========================\n      Applications (2x2 cards)\n  ========================== *\/\n  .biofargo-grid{\n    display:grid;\n    grid-template-columns:repeat(2, minmax(0, 1fr));\n    gap:16px;\n  }\n  .biofargo-app-card{\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:14px;\n    background:#fff;\n  }\n  .biofargo-app-card h4{\n    margin:0 0 6px 0;\n    font-size:16px;\n    color:#333;\n  }\n  .biofargo-app-card p{\n    margin:0;\n    font-size:14px;\n  }\n  @media (max-width:640px){\n    .biofargo-wrap{ padding:18px; }\n    .biofargo-grid{ grid-template-columns:1fr; }\n  }\n\n  \/* =========================\n      Warning \/ Cautions\n  ========================== *\/\n  .biofargo-caution{\n    background:#fff5f5;\n    \n    padding:14px;\n    border-radius:10px;\n    color:#852d2d;\n    font-size:14px;\n  }\n  .biofargo-caution p{ margin:0 0 8px 0; }\n  .biofargo-caution p:last-child{ margin:0; }\n\n  \/* =========================\n      Documents (dual CTA)\n  ========================== *\/\n  .biofargo-docs{\n    display:flex;\n    flex-wrap:wrap;\n    gap:12px;\n    margin-top:12px;\n  }\n  .biofargo-btn{\n    display:inline-block;\n    padding:10px 18px;\n    border-radius:8px;\n    font-weight:800;\n    font-size:14px;\n    text-decoration:none;\n  }\n  .biofargo-btn.primary{\n    background:#0056b3;\n    color:#fff;\n    border:2px solid #0056b3;\n  }\n  .biofargo-btn.outline{\n    background:#fff;\n    color:#0056b3;\n    border:2px solid #0056b3;\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\u003c!-- Description --\u003e\n\u003cdiv style=\"margin-top: 0;\" class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDescription \u003c\/h2\u003e\n\u003cdiv class=\"biofargo-card\"\u003e\n\u003cp style=\"margin: 0 0 10px 0;\"\u003eRecombinant human erythropoietin (EPO) is a glycosylated cytokine essential for erythropoiesis, specifically promoting the proliferation and differentiation of erythroid progenitor cells (CFU-E) into mature erythrocytes.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 10px 0;\"\u003eWhile renal production maintains physiological EPO levels in adults, hepatic synthesis predominates during fetal and neonatal development.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 10px 0;\"\u003eBeyond its canonical role in hematopoiesis, EPO exhibits pleiotropic bioactivities, including angiogenic stimulation via vascular smooth muscle cell proliferation, neuroprotective effects under hypoxic conditions, and modulation of B-cell function.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003eThe recombinant protein retains native conformational integrity, ensuring targeted activation of the EPO receptor (EPOR) to orchestrate tissue-specific responses without off-target interactions, making it invaluable for therapeutic applications in anemia treatment and regenerative medicine.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Specifications --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"width: 25%;\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"width: 35%;\"\u003eAcceptance Criteria\u003c\/th\u003e\n\u003cth style=\"width: 20%;\" class=\"center\"\u003eTL-636-0050\u003c\/th\u003e\n\u003cth style=\"width: 20%;\" class=\"center\"\u003eTL-636-0100\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eAppearance\u003c\/td\u003e\n\u003ctd\u003eFreeze-dried preparation, the form should be white loose body\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIdentity\u003c\/td\u003e\n\u003ctd\u003eThe electrophoretic bands should be consistent with the molecular weight of the product\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eVisible Particle\u003c\/td\u003e\n\u003ctd\u003eShould be no visible particle\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMoisture\u003c\/td\u003e\n\u003ctd\u003e≤4.0%\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e2.0%\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e2.9%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003epH\u003c\/td\u003e\n\u003ctd\u003e6.5~7.5\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e7.3\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e7.3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurity\u003c\/td\u003e\n\u003ctd\u003e\u0026gt;90% \/ SDS-PAGE\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eCompliance\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e\u0026lt;0.1EU\/μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMycoplasma\u003c\/td\u003e\n\u003ctd\u003eShould be negative\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eNegative\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eNegative\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSterility\u003c\/td\u003e\n\u003ctd\u003eNo growth\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eNo growth\u003c\/td\u003e\n\u003ctd class=\"center\"\u003eNo growth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiological Activity\u003c\/td\u003e\n\u003ctd\u003eCell proliferation assay with TF-1 cells, specific activity ≥1×10\u003csup\u003e6\u003c\/sup\u003e IU\/mg\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e3.08×10\u003csup\u003e6\u003c\/sup\u003e IU\/mg\u003c\/td\u003e\n\u003ctd class=\"center\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Features --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n\u003cul class=\"biofargo-checklist\"\u003e\n\u003cli\u003e\n\u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eGlycosylated, Mammalian-Derived EPO\u003c\/strong\u003e\n\u003cp\u003eHEK293 expression preserves native glycosylation and EPOR-specific signaling fidelity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eValidated High Biological Activity\u003c\/strong\u003e\n\u003cp\u003eDemonstrated potency in TF-1 cell proliferation assay (≥1.0 × 10⁶ IU\/mg).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Purity with Stringent QC\u003c\/strong\u003e\n\u003cp\u003e90% purity by SDS-PAGE with confirmed sterility, mycoplasma negativity, and low endotoxin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eConsistent Lot-to-Lot Performance\u003c\/strong\u003e\n\u003cp\u003eControlled moisture, pH, and activity ensure reproducibility in hematopoietic research workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Applications --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-grid\"\u003e\n\u003cdiv class=\"biofargo-app-card\"\u003e\n\u003ch4\u003eErythropoiesis Research\u003c\/h4\u003e\n\u003cp\u003ePromotes proliferation and differentiation of erythroid progenitors (CFU-E).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-app-card\"\u003e\n\u003ch4\u003eT Cell-Related Studies\u003c\/h4\u003e\n\u003cp\u003eApplied as a functional cytokine component in immune cell research systems.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-app-card\"\u003e\n\u003ch4\u003eCIK Cell Research\u003c\/h4\u003e\n\u003cp\u003eUsed in cytokine-induced killer (CIK) cell culture and functional modulation studies.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"biofargo-app-card\"\u003e\n\u003ch4\u003eRegenerative \u0026amp; Angiogenesis Research\u003c\/h4\u003e\n\u003cp\u003eExplored for non-hematopoietic roles including angiogenesis and tissue protection under hypoxia.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Stability \u0026 Storage --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eStability \u0026amp; Storage\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px; margin: 10px 0 0 0;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eLyophilized powder: Stable for 12 months at −80 °C or 6 months at −20 °C when stored in the original sealed container under desiccant.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eReconstitution: Dissolve in sterile Water for Injection, 0.9% NaCl, or PBS (pH 7.4), maintaining a final concentration ≥100 µg\/mL to prevent adsorption.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 0;\"\u003eHandling: Aliquot to avoid repeated freeze–thaw cycles.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c!-- Cautions (Universal) --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eCautions\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-caution\"\u003e\n\u003cp\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not intended for diagnostic or therapeutic use.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e• Handling:\u003c\/strong\u003e Prepare solutions using sterile technique. Avoid microbial contamination to maintain activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e• Storage:\u003c\/strong\u003e Follow recommended storage conditions. Repeated freeze–thaw cycles may reduce performance.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!-- Documents --\u003e\n\u003cdiv class=\"biofargo-section\"\u003e\n\u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-docs\"\u003e\n\u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-Recombinant_Human_EPO_Protein.pdf?v=1772435758\" class=\"biofargo-btn primary\" target=\"_blank\"\u003e📄 TL-636 Data Sheet\u003c\/a\u003e \u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-Recombinant_Human_EPO_Protein.pdf?v=1772435759\" class=\"biofargo-btn outline\" target=\"_blank\"\u003e📄 TL-636 SDS\u003c\/a\u003e \u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-Pr2410007-TL-636-0050.pdf?v=1772435758\" class=\"biofargo-btn outline\" target=\"_blank\"\u003e📄 TL-636-0050 COA\u003c\/a\u003e \u003ca rel=\"noopener\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-Pr2306009-TL-636-0100.pdf?v=1772435758\" class=\"biofargo-btn outline\" target=\"_blank\"\u003e📄 TL-636-0100 COA\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"50μg","offer_id":43214480375989,"sku":"TL-636-0050","price":220.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43214480408757,"sku":"TL-636-0100","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Recombinant_Human_EPO_Protein.jpg?v=1775112623"},{"product_id":"hla-a-02-01","title":"HLA-A*02:01","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eSerological Name:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eA2\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003ePeptide Load:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eEndogenous\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eSize:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e30.0 µg\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eModification:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eUnmodified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eVolume:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003e30.0 µl\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eConjugate:\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"short-description\"\u003e\u003c\/div\u003e\n\u003cp data-mce-fragment=\"1\" class=\"short-description\"\u003e \u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\" class=\"short-description\"\u003eRecombinant, truncated Class I soluble Human Leukocyte Antigen (sHLA), naturally folded and glycosylated, loaded with endogenous peptides.\u003c\/p\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attributes\"\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALLELE NAME\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA*02:01, B2m\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eSEROLOGICAL NAME\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA2\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALLELE FREQUENCY - WORLDWIDE POPULATION\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003e12.0%\/–\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eALTERNATIVE NAMES\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eA*0201, A0201, A*02, A02, A2\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eRECOMMENDED ANTIBODIES\u003c\/label\u003e\u003c\/h3\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eW6\/32, Anti-B2m\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\n\u003cdiv data-mce-fragment=\"1\" class=\"product-attribute\"\u003e\n\u003ch3 data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003clabel data-mce-fragment=\"1\" class=\"catalog_list-item__attribute-head\"\u003eAPPLICATIONS\u003c\/label\u003e\u003c\/h3\u003e\n\u003cp data-mce-fragment=\"1\" class=\"eq_height\"\u003e\u003cspan data-mce-fragment=\"1\"\u003eHLA Sandwich ELISAs, HLA Direct ELISAs, HLA-Sera Antibody Assays, HLA Controls in ELISA Assays, HLA Assay Standards, HLA Blocking Assays, HLA Neutralizing Assays, HLA Competition Assays, HLA Bead Assays, HLA Immunization Procedures\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 234px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eConjugate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eUnconjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComplexity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eMonomer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHLA Species\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHost Cell Line Species\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eClone Designation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eOB11\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eTail Specification\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eVLDL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eProtein Format\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eRecombinant, truncated, soluble, non-modified, non-labeled, endogenously loaded\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eStorage Temperature\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e4˚C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1000.0 µg\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003ePBS\/0.02% Sodium Azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003epH\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eResearch Use Only [RUO]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAuthentication Method\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSequence Based Typing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eSequences\u003c\/h2\u003e\n\u003ch3\u003eALPHA CHAIN SEQUENCE\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eGSHSMRYFFT SVSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASQRMEPRAP WIEQEGPEYW DGETRKVKAH SQTHRVDLGT LRGYYNQSEA GSHTVQRMYG CDVGSDWRFL RGYHQYAYDG KDYIALKEDL RSWTAADMAA QTTKHKWEAA HVAEQLRAYL EGTCVEWLRR YLENGKETLQ RTDAPKTHMT HHAVSDHEAT LRCWALSFYP AEITLTWQRD GEDQTQDTEL VETRPAGDGT FQKWAAVVVP SGQEQRYTCH VQHEGLPKPL TLRSVVSTDD DLA\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003eBETA CHAIN SEQUENCE\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eIQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cbr\u003e\u003c\/h2\u003e","brand":"hlaprotein","offers":[{"title":"Default Title","offer_id":43193412878517,"sku":"A0201","price":773.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/a0201_hla1_3d_r_2.png?v=1732874223"},{"product_id":"recombinant-human-il-3-protein","title":"Recombinant Human IL-3 Protein","description":"\u003cstyle\u003e\n  \/* =========================\n     Table Responsive (same logic as reference)\n  ========================== *\/\n\n  .biofargo-table-wrapper {\n    overflow-x: auto;\n    margin: 20px 0;\n    -webkit-overflow-scrolling: touch;\n  }\n\n  .biofargo-spec-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #dee2e6;\n    min-width: 600px;   \/* Desktop keeps normal layout *\/\n    background: #fff;\n  }\n\n  .biofargo-spec-table th,\n  .biofargo-spec-table td {\n    border: 1px solid #dee2e6;\n    padding: 12px;\n    vertical-align: middle;\n    white-space: normal;\n    word-break: break-word;\n  }\n\n  @media (max-width: 600px) {\n    .biofargo-table-wrapper {\n      border: 1px solid #eee;\n      border-radius: 8px;\n      padding: 6px;\n      background: #fff;\n    }\n\n    .biofargo-spec-table {\n      min-width: 720px; \/* Mobile horizontal scroll *\/\n    }\n  }\n\u003c\/style\u003e\n\u003cdiv style=\"font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1000px; margin: 0 auto; border: 1px solid #e1e8ed; padding: 30px; border-radius: 10px; background-color: #ffffff;\"\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 0; font-size: 22px;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fcfcfc; padding: 15px; border-radius: 6px; border: 1px solid #f0f0f0;\"\u003e\n\u003cp style=\"margin: 0; text-align: justify;\"\u003eRecombinant Human Interleukin-3 (IL-3) is a crucial cytokine involved in the regulation of hematopoiesis, stimulating the proliferation and differentiation of various blood cells. IL-3 exerts its effects by binding to the IL-3 receptor, initiating signaling pathways that are essential for the development and function of the immune system. This makes it a valuable tool for research in immunology, hematology, and cell biology.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrapper\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #0056b3; color: white;\"\u003e\n\u003cth style=\"padding: 12px; text-align: left;\" colspan=\"2\"\u003eCytokines Details\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eExpression Host\u003c\/td\u003e\n\u003ctd\u003eA DNA sequence encoding the human IL-3 (GenBank: AAA59146.1) was expressed with a His-tag at the C-terminus.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eSynonym\u003c\/td\u003e\n\u003ctd\u003eMCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eProtein Sequence\u003c\/td\u003e\n\u003ctd\u003eThe recombinant human IL-3 protein consists of 139 amino acids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eMolecular Mass\u003c\/td\u003e\n\u003ctd\u003ePredicts a molecular mass of 15.9 kD.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eQC Testing Purity\u003c\/td\u003e\n\u003ctd\u003e\u0026gt; 95% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eEndotoxin\u003c\/td\u003e\n\u003ctd\u003eHEK293 cells (Host verification)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eFormulation\u003c\/td\u003e\n\u003ctd\u003eLyophilized powder.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eStability\u003c\/td\u003e\n\u003ctd\u003eStore at -20°C or below to maintain product stability and activity. Avoid repeated freeze-thaw cycles.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"font-weight: bold; background-color: #f8f9fa;\"\u003eStorage\u003c\/td\u003e\n\u003ctd\u003eStable for long-term storage under recommended conditions, ensuring reliable performance.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Purity:\u003c\/strong\u003e This product undergoes stringent purification processes, achieving a purity level of over 95%, ensuring the reliability and reproducibility of experimental results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eHigh Biological Activity:\u003c\/strong\u003e Verified through multiple activity assays, this product effectively induces the expected biological responses in target cells.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eBatch Consistency:\u003c\/strong\u003e Manufactured under strict production and quality control protocols to ensure high consistency between batches.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 30px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #0056b3; font-weight: bold;\"\u003e✔\u003c\/span\u003e \u003cstrong\u003eAnimal-Free:\u003c\/strong\u003e Produced without the use of animal-derived components, meeting animal-free requirements.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eApplications\u003c\/h2\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: 1fr 1fr; gap: 20px;\"\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eHematology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eResearch the role of IL-3 in the regulation of blood cell production and mechanisms of hematopoiesis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eImmunology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eStudy the effects of IL-3 on immune cell development and function in immune response.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eStem Cell Research\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eUtilize IL-3 in studies focusing on stem cell differentiation and proliferation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"border: 1px solid #e1e8ed; padding: 15px; border-radius: 8px;\"\u003e\n\u003ch4 style=\"margin-top: 0; color: #333;\"\u003eCell Biology\u003c\/h4\u003e\n\u003cp style=\"font-size: 14px; margin-bottom: 0;\"\u003eInvestigate the impact of IL-3 on cell signaling pathways, survival, and proliferation.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eData Figures\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin-top: 20px; border: 1px solid #eee; padding: 15px; border-radius: 8px;\"\u003e\n\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"Recombinant Human IL-3 Protein\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_e48a1ebe-c991-4b61-917e-6beac15bb84e_600x600.jpg?v=1768809296\"\u003e\n\u003cp style=\"font-size: 13px; color: #888; margin-top: 10px;\"\u003eFigure 1: Recombinant Human IL-3 Protein Analysis\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eInstructions for Use\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDissolve the lyophilized powder in an appropriate sterile buffer in a sterile environment before use.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003ePrepare and dilute the solution according to experimental requirements to ensure accurate and reproducible results.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eDo not mix with other similar products to avoid cross-reactivity and potential interference.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eCautions\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #fff5f5;  padding: 15px; color: #852d2d; font-size: 14px;\"\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Research Use Only:\u003c\/strong\u003e This product is not suitable for clinical diagnostics or therapeutic applications.\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e\u003cstrong\u003e• Preservation:\u003c\/strong\u003e Handle according to instructions to preserve its activity and stability.\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cstrong\u003e• Support:\u003c\/strong\u003e Contact our technical support team if you encounter any issues or questions.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #0056b3; border-bottom: 2px solid #0056b3; padding-bottom: 8px; margin-top: 30px; font-size: 22px;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"display: flex; gap: 15px; margin-top: 10px; flex-wrap: wrap;\"\u003e\n\u003ca style=\"display: inline-block; padding: 10px 20px; background-color: #0056b3; color: white; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-Recombinant_Human_IL-3_Protein.pdf?v=1772515503\" target=\"_blank\"\u003e📄 GMP-TL511 Data Sheet\u003c\/a\u003e \u003ca style=\"display: inline-block; padding: 10px 20px; border: 2px solid #0056b3; color: #0056b3; text-decoration: none; border-radius: 5px; font-weight: bold; font-size: 14px;\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-Recombinant_Human_IL-3_Protein.pdf?v=1772515503\" target=\"_blank\"\u003e📄 GMP-TL511 SDS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"50μg","offer_id":43214488895669,"sku":"GMP-TL511-0050","price":260.0,"currency_code":"USD","in_stock":true},{"title":"100μg","offer_id":43214488928437,"sku":"GMP-TL511-0100","price":460.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG-GMP-TL511-0050.jpg?v=1772515493"},{"product_id":"mycguard-1-solution-1000-for-disinfecting-external-water-bath","title":"MycGuard™ MycGuard-2 Solution (500x), for Disinfecting Ordinary Water Bath","description":"\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNotes： \u003c\/strong\u003eIt is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMycoplasma contamination not only involves places that directly contact cells such as cell culture plates, culture reagents, or ultra-clean benches but also equipment such as water trays and water baths in cell culture incubators that may cause mycoplasma contamination. In addition to providing scavenging agents that directly remove mycoplasma agents, normal cells are also provided with mycoplasma preventive agents to prevent mycoplasma contamination. It mainly includes the following aspects: reagent prevention (directly added to the cell culture medium), ultra-clean bench prevention (spray), water bath prevention (directly added to the water bath) cell incubator water tray prevention, etc.\u003c\/p\u003e\n\u003cp\u003eIt is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths. Its mechanism of action involves physically breaking down the cell walls of microorganisms and inhibiting the synthesis of enzymes required for their reproduction, ultimately leading to microbial death. As a result, this product does not induce antibiotic resistance. Multiple independent validation tests have shown that this product effectively clears various types of bacteria, fungi, mycoplasma, and other microorganisms. It is especially effective in inhibiting airborne bacteria, fungi, spores, and spore-forming bacteria for up to 7-10 days It is provided in the form of a 500× concentrated solution. It can be directly added to the water bath according to the proportion when using it.\u003c\/p\u003e\n\u003ch2\u003eKey Feature\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad-Spectrum and Highly Effective:\u003c\/strong\u003e Specifically designed to eliminate microbial contamination in water baths, including bacteria, fungi, and mycoplasma, without the risk of bacterial resistance development.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Safety Profile:\u003c\/strong\u003e Non-irritating, non-corrosive, and non-volatile. Completely degrades within 7-10 days. Mouse and rabbit feeding and irritation tests have shown no adverse reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient to Use:\u003c\/strong\u003e Easy to operate, saving time and effort.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstruction\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWear latex gloves during operation.\u003c\/li\u003e\n\u003cli\u003eAdd the product directly to the water bath at a ratio of 1:500, treating every 7-10 days.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003e40610es, antimicoplasma\u003c\/div\u003e","brand":"Yeasenbio","offers":[{"title":"100 mL","offer_id":43256824660149,"sku":"40610ES60","price":109.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/my-2.jpg?v=1756374955"},{"product_id":"eosin-staining-solution","title":"Eosin Staining Solution-Biosci™","description":"\u003cp\u003e\u003cstrong\u003eNote:It is mainly used for staining the cytoplasm.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eModel \u0026amp; Specification\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 98.7888%; height: 54px;\" data-mce-style=\"border-collapse: collapse; width: 98.7888%; height: 54px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eCatalog#\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eModel \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eDescription \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003ePack Size\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e4960211\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003eAlcohol-soluble\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003eAlcoholic solution of Eosin\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e500mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 19.3168%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.3168%; height: 18px; text-align: center;\"\u003e4960221\u003c\/td\u003e\n\u003ctd style=\"width: 19.5587%; height: 18px; text-align: center;\" data-mce-style=\"width: 19.5587%; height: 18px; text-align: center;\"\u003eWater-soluble\u003c\/td\u003e\n\u003ctd style=\"width: 36.1245%; height: 18px; text-align: center;\" data-mce-style=\"width: 36.1245%; height: 18px; text-align: center;\"\u003eAqueous solution of Eosin\u003c\/td\u003e\n\u003ctd style=\"width: 23.8086%; height: 18px; text-align: center;\" data-mce-style=\"width: 23.8086%; height: 18px; text-align: center;\"\u003e500mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eIntroduction\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe nucleus in the cell has a certain degree of acidity, and it has a strong affinity with the basic stain (hematoxylin), while the cytoplasm is on the contrary, which has a certain degree of basicity and a strong affinity with the acid stain (eosin). After cell smears or tissue sections are subject to hematoxylin-eosin staining, the nucleus will be stained blue-purple by hematoxylin, cytoplasm, muscle fibers, collagen fibers, etc. will be red in varying degrees, and red blood cells will be orange-red.\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/1_97c16094-fbbc-4b3a-8ee9-b526f79ad0d5.png?v=1719362062\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/1_97c16094-fbbc-4b3a-8ee9-b526f79ad0d5.png?v=1719362062\"\u003e     \u003cimg style=\"font-size: 0.875rem;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ad1a1b7183951dc89f508a6b6c422144_96d4d679-e49b-46a1-8456-3054ade7181a.png?v=1719362062\" data-mce-style=\"font-size: 0.875rem;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/ad1a1b7183951dc89f508a6b6c422144_96d4d679-e49b-46a1-8456-3054ade7181a.png?v=1719362062\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eMain Compositions\u003c\/h2\u003e\n\u003cp\u003eEosin, Ethanol, Acetic acid\u003cspan style=\"font-size: 0.875rem;\" data-mce-style=\"font-size: 0.875rem;\"\u003e  \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eSample Requirement\u003c\/h2\u003e\n\u003cp\u003eSmears and sections must be fully fixed, and paraffin sections must be fully deparaffinized.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProcedures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eDeparaffinize the sections for 5-10 min in xylene I, II and III respectively, and then transfer into 100% and 95% ethanol respectively for 1-3 min each;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmerse in 85% ethanol for 1-3 min and rinse with tap water for rehydration for 1-3 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStain in Hematoxylin Staining Solution for 3-5 min, and rinse with tap water for 1-2 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDifferentiate in Differentiation Solution for 1-10 sec; and rinse with tap water for 2min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBluing with running water for 5-10 min; or bluing with bluing solution for 1-3 min then rinse with tap water for 1-3 min;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eImmerse in Eosin Staining Solution (Water-soluble) for 30-60 sec; or dehydrate in 85% ethanol, then stain in Eosin Staining Solution (Alcohol-soluble) for 10-30 sec (Water washing should be avoided);\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdjust color for 2 min in 95% ethanol I and II respectively;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDehydrate for 1-3 min through 100% ethanol I and II respectively, and clear in xylene I and II for 1-3 min respectively;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMount with mounting medium and coverslips, observe the result under microscope.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 data-mce-fragment=\"1\"\u003eMaterials Required But Not Provided\u003c\/h2\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAppropriate material is required for immunohistochemical (IHC) staining protocol, such as graded alcohols, xylene or xylene substitutes, hematoxylin staining solution, differentiation solution, bluing solution, etc.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003c\/p\u003e\n\u003ch2\u003eInterpretation of Test Results\u003c\/h2\u003e\n\u003cp\u003eNuclei show blue purple. Cytoplasm, intercellular substance and fibrin show pink to red. And the red cells would show orange red.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eFor professional use only;\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe user shall operate in strict accordance with the instructions; Work clothes, gloves and masks shall be wore when operating, and the operating environment shall be ventilated as possible.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe prepared smears or sections shall be fully fixed. Paraffin sections shall be fully deparaffinized, otherwise non-staining or uneven staining will be caused.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe Differentiation Solution shall not be used for too long. The smear or section should be washed with clear water immediately when it turn pink.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe differentiation time shall not be too long, and the smears or sections shall be rinsed with clear water immediately after they turn pink.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStaining time can be properly shorten in summer or at higher temperature, and it can be properly prolonged in winter or at lower temperature.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAfter staining with Eosin, should not wash with ethanol for too long, otherwise it would cause decolorization of eosin.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eChange the gradient ethanol regularly to ensure the purity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis staining solution is for in vitro diagnostic (IVD) use only and it needs to be used within the validity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eDispose of all containers, waste liquid and slides in accordance with national regulations, including unused items and used items.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eLimitations\u003c\/h2\u003e\n\u003cp\u003eOnly for staining of cell morphology and histomorphology observation. The staining time and staining quality are affected by temperature, season, operator's experience, sample preparation and section quality.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDisposal\u003c\/h2\u003e\n\u003cp\u003eWaste must be disposed of in compliance with local legislation. Take the appropriate precautions for infected material if necessary.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Conditions and Expiry Date\u003c\/h2\u003e\n\u003cp\u003eStorage at 10℃-30℃, valid for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Brochure_for_Staining_Solution.pdf?v=1699941846\" data-mce-href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Brochure_for_Staining_Solution.pdf?v=1699941846\" type=\"application\/pdf\" target=\"_blank\"\u003eBrochure for Staining Solution\u003c\/a\u003e\u003c\/p\u003e\n\u003col\u003e\u003c\/ol\u003e","brand":"Dakewe","offers":[{"title":"Water-soluble","offer_id":43270741590197,"sku":"4960221","price":168.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4960221-EosinStainingSolution_proc.jpg?v=1732871665"},{"product_id":"mouse-lymphocyte-separation-medium","title":"Mouse Lymphocyte Separation Medium-Biosci™","description":"\u003cstyle\u003e\n    .bf-container {\n        font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif;\n        color: #333;\n        line-height: 1.6;\n        max-width: 1200px;\n        margin: 0 auto;\n        padding: 20px;\n    }\n\n    .bf-container h2 {\n        \n        padding-left: 15px;\n        background: #f8f9fa;\n        padding-top: 10px;\n        padding-bottom: 10px;\n        margin-top: 30px;\n        font-size: 1.5rem;\n        color: #0056b3;\n        text-transform: uppercase;\n        letter-spacing: 0.5px;\n    }\n\n    .bf-note-box {\n        background-color: #fff4e5;\n        border: 1px solid #ffcc80;\n        padding: 15px;\n        border-radius: 4px;\n        margin-bottom: 20px;\n    }\n\n    .bf-table-wrapper {\n        overflow-x: auto;\n        margin: 20px 0;\n    }\n\n    .bf-table {\n        width: 100%;\n        border-collapse: collapse;\n        margin-bottom: 1rem;\n        background-color: transparent;\n    }\n\n    .bf-table th, .bf-table td {\n        padding: 12px 15px;\n        border: 1px solid #dee2e6;\n        text-align: left;\n    }\n\n    .bf-table tr:nth-child(even) {\n        background-color: #fcfcfc;\n    }\n\n    .bf-table td:first-child {\n        font-weight: bold;\n        background-color: #f8f9fa;\n        width: 25%;\n    }\n\n    .bf-image-container {\n        text-align: center;\n        margin: 30px 0;\n    }\n\n    .bf-image-container img {\n        max-width: 100%;\n        height: auto;\n        border: 1px solid #eee;\n        border-radius: 8px;\n        box-shadow: 0 2px 10px rgba(0,0,0,0.05);\n    }\n\n    .bf-caption {\n        display: block;\n        font-style: italic;\n        color: #666;\n        margin-top: 10px;\n        font-size: 0.9rem;\n    }\n\n    .bf-download-card {\n        display: flex;\n        align-items: center;\n        background: #ffffff;\n        border: 2px solid #0056b3;\n        border-radius: 8px;\n        padding: 20px;\n        margin: 20px 0;\n        transition: transform 0.2s;\n        text-decoration: none !important;\n    }\n\n    .bf-download-card:hover {\n        transform: translateY(-2px);\n        box-shadow: 0 4px 12px rgba(0,0,0,0.1);\n    }\n\n    .bf-download-icon {\n        font-size: 40px;\n        margin-right: 20px;\n        color: #0056b3;\n    }\n\n    .bf-download-text h4 {\n        margin: 0;\n        color: #0056b3;\n        font-size: 1.1rem;\n    }\n\n    .bf-download-text p {\n        margin: 5px 0 0;\n        color: #666;\n        font-size: 0.85rem;\n    }\n\n    .bf-container ul {\n        padding-left: 20px;\n    }\n\n    .bf-container li {\n        margin-bottom: 10px;\n    }\n\u003c\/style\u003e\n\n\u003cdiv class=\"bf-container\"\u003e\n\n    \u003ch2\u003eDescription\u003c\/h2\u003e\n    \u003cp\u003eMouse Lymphocyte Separation Medium is a new generation of density gradient separation medium. The main component is iodixanol, with a molecular weight of 1,550. It is a completely chemically inert, non-biotoxic iodide that does not bind with any known biological function protein, does not interfere with any cell surface membrane protein, does not inhibit enzyme activity and does not interfere with antigen-antibody responses.\u003cbr\u003eThe lymphocyte separated by this product is with high purity, good state and high yield. The operation of Mouse Lymphocyte Separation Medium is simple, easy to learn and does not need too much experience.\u003c\/p\u003e\n    \u003cp\u003eOur study showed that the number and quality of mouse’s spleen lymphocytes did not change significantly after 1 hour of exposure to the separation medium, and the subsequent ELISPOT test results were completely consistent with those of the control group.\u003c\/p\u003e\n\n    \u003ch2\u003eSpecification\u003c\/h2\u003e\n    \u003cdiv class=\"bf-table-wrapper\"\u003e\n        \u003ctable class=\"bf-table\"\u003e\n            \u003ctbody\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eProduct name\u003c\/td\u003e\n                    \u003ctd\u003eMouse Lymphocyte Separation Medium\u003c\/td\u003e\n                    \u003ctd\u003eSize\u003c\/td\u003e\n                    \u003ctd\u003e100mL\/bottle\u003cbr\u003e250mL\/bottle\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eApplication\u003c\/td\u003e\n                    \u003ctd\u003eSeparation of mouse\/rat spleen lymphocytes\u003cbr\u003eSeparation of mouse\/rat\/rabbit PBMCs\u003c\/td\u003e\n                    \u003ctd\u003eEndotoxin\u003c\/td\u003e\n                    \u003ctd\u003e\u0026lt; 0.5EU\/mL\u003c\/td\u003e\n                \u003c\/tr\u003e\n                \u003ctr\u003e\n                    \u003ctd\u003eDensity\u003c\/td\u003e\n                    \u003ctd\u003e(1.081±0.001) g\/mL (20 °C)\u003c\/td\u003e\n                    \u003ctd\u003eOsmolality\u003c\/td\u003e\n                    \u003ctd\u003e(280±15)mOsmol\/kg\u003c\/td\u003e\n                \u003c\/tr\u003e\n            \u003c\/tbody\u003e\n        \u003c\/table\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003eStorage\u003c\/h2\u003e\n    \u003cp\u003e\u003cstrong\u003eStorage:\u003c\/strong\u003eThe product shall be stored at 2 °C -30 °C and protected from light. Its shelf life is 2 years.\u003c\/p\u003e\n\n    \u003ch2\u003eMaterials Required But Not Provided\u003c\/h2\u003e\n    \u003cp\u003e35mm Petri dishes, the plunger of a 10mL glass syringe, 70µm cell strainer or nylon mesh with 200 meshes - cut into 90mm×90mm square (materials above are all sterile), centrifuge tubes, pipettes, tips, centrifuge with a swing-out rotor, etc.\u003c\/p\u003e\n\n    \u003ch2\u003eProtocol\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eSacrifice the mouse by cervical dislocation; dip it in 75% ethanol.\u003c\/li\u003e\n        \u003cli\u003eTake out the spleen of the mouse at a clean bench. Operation shall be performed in an aseptic condition.\u003c\/li\u003e\n        \u003cli\u003eAdd 4-5 mL Mouse Lymphocyte Separation Medium (pre-warming at room temperature and shaking before use) into a 35 mm petri dish. And grind (please refer to Figure 2 for grinding operation).\u003c\/li\u003e\n        \u003cli\u003eTransfer the cell suspension to a 15mL centrifuge tube immediately (refer to Figure 1(A)), and cover with 500-1000μl RPMI 1640 medium (keeping liquid level boundary clear). Please refer to Figure 1(B).\u003c\/li\u003e\n        \u003cli\u003eCentrifuge at 800g for 30min in a swing-out rotor at room temperature. The acceleration and deceleration are set to a slower speed (set to third gear if ten gears are available). The lymphocytes after centrifugation are shown in Figure 1(C).\u003c\/li\u003e\n        \u003cli\u003eTransfer the lymphocyte layer at the interface to a new centrifuge tube. Wash the cells with 10mL RPMI 1640 medium, and centrifuge at 250g for 10min.\u003c\/li\u003e\n        \u003cli\u003eDiscard the supernatant, resuspend the cells in culture medium and count.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cdiv class=\"bf-image-container\"\u003e\n        \u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115154610_480x480.png?v=1700034383\" target=\"_blank\"\u003e\n            \u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115154610_480x480.png?v=1700034383\" style=\"cursor: zoom-in;\"\u003e\n        \u003c\/a\u003e\n    \u003c\/div\u003e\n\n    \u003ch2\u003eCautions\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eIf the mouse is fed for a long time or the spleen is enlarged abnormally, which causes that the cell suspension after grinding shows dull red, please dilute the cells suspension with an equal volume of Mouse Lymphocyte Separation Medium and mix it well, and then proceed to step 4.\u003c\/li\u003e\n        \u003cli\u003eThe separation medium is volatile so the grinding time shall be controlled within 5 min.\u003c\/li\u003e\n        \u003cli\u003eOnce opened, please store the separation medium at 2-8℃ to avoid the change of density of the separation solution caused by liquid volatilization, which will affect the separation effect.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSeparation of the spleen lymphocyte of the rat:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cp\u003eBecause the rat’s spleen is large, only a small portion of it needs to be cut for the experiment. The method of grinding and separation is exactly the same as that for the separation of mouse’s spleen lymphocytes.\u003c\/p\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSeparation of mouse\/rat\/rabbit blood lymphocyte:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cul\u003e\n        \u003cli\u003eCollect 0.5-3mL anticoagulant blood of mouse\/rat\/rabbit, dilute it with an equal volume of RPMI 1640 medium or PBS. (Separation is more effective after hemodilution. Operation shall be performed in an aseptic condition)\u003c\/li\u003e\n        \u003cli\u003eAdd 3mL of Mouse Lymphocyte Separation Medium to a 15 mL centrifuge tube. Carefully layer the diluted blood over 3mL Mouse Lymphocyte Separation Medium in a 15mL centrifuge tube, avoid mixing the interface.\u003c\/li\u003e\n        \u003cli\u003eCentrifuge at 800g for 15-20min in a swing-out rotor at room temperature. The acceleration and deceleration are set to a slower speed (set to third gear if ten gears are available).\u003c\/li\u003e\n        \u003cli\u003eThe subsequent steps are the same as the mouse’s spleen lymphocyte separation operation.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003cp\u003e\u003cstrong\u003eSpecial instruction - Mouse’s spleen grinding:\u003c\/strong\u003e\u003c\/p\u003e\n    \u003cul\u003e\n        \u003cli\u003eIt is recommended to use nylon mesh, because it is more flexible.\u003c\/li\u003e\n        \u003cli\u003eIt is recommended to use the plunger of syringe to grind the spleen.\u003c\/li\u003e\n        \u003cli\u003eThe key is taking advantage of the rebound to control grinding, which can minimize the mechanical damage of the cells.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eNotes\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eControl the grinding strength to keep the nylon mesh suspended, to avoid a large number of cell death caused by direct grinding on the bottom of the petri dish.\u003c\/li\u003e\n        \u003cli\u003eThe petri dish cannot be too large, otherwise the nylon mesh cannot have effective elasticity.\u003c\/li\u003e\n        \u003cli\u003eClamp the nylon mesh with tweezers on one side to prevent the nylon net from sliding during grinding.\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eReferences\u003c\/h2\u003e\n    \u003cul\u003e\n        \u003cli\u003eLili Liu, et al.American Journal of Nephrology. 2012; 36(2): 386-396\u003c\/li\u003e\n        \u003cli\u003eLi Zhang, et al. Iranian Journal of Allergy, Asthma and Immunology. 2012; 11(2): 133-145\u003c\/li\u003e\n        \u003cli\u003eJianHui Xiao,et al.Evidence-Based Complementary and Alternative Medicine.2012;ArticleID 273435, 15 pages\u003c\/li\u003e\n    \u003c\/ul\u003e\n\n    \u003ch2\u003eDocument\u003c\/h2\u003e\n    \u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/7211011_7211012_BioSci_Mouse_Lymphocyte_Separation_Medium.pdf?v=1700036452\" class=\"bf-download-card\" target=\"_blank\"\u003e\n        \u003cdiv class=\"bf-download-icon\"\u003e📄\u003c\/div\u003e\n        \u003cdiv class=\"bf-download-text\"\u003e\n            \u003ch4\u003eMouse Lymphocyte Separation Medium\u003c\/h4\u003e\n            \u003cp\u003eClick to download the PDF technical document (Instruction for Use)\u003c\/p\u003e\n        \u003c\/div\u003e\n    \u003c\/a\u003e\n\n\u003ch2\u003eRelated Products\u003c\/h2\u003e\n\u003cdiv class=\"bf-note-box\"\u003e\n\u003cstrong\u003eRelated Product Options:\u003c\/strong\u003e\n\u003cp\u003eSelect the appropriate format for your PBMC isolation workflow. Choose from separation medium, empty tubes, or ready-to-use prefilled tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(260px, 1fr)); gap: 20px; margin-top: 20px;\"\u003e\n\u003c!-- Empty Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eEMPTY TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003ePBMC Separation Tube (Empty)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/15-ml-50-ml-lymphocyte-separation-tube-with-porous-barrier-for-pbmc-isolation?variant=52452569186485\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Human Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eHuman Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/human-lymphocyte-separation-medium?variant=43275893735605\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Mouse Medium --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003eSEPARATION MEDIUM\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eMouse Lymphocyte Separation Medium\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/mouse-lymphocyte-separation-medium?variant=43275988598965\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c!-- Prefilled Tube --\u003e\n\u003cdiv style=\"border: 1px solid #e5e7eb; border-radius: 10px; padding: 18px; background: #fff;\"\u003e\n\u003cdiv style=\"font-size: 12px; color: #6b7280; margin-bottom: 6px;\"\u003ePREFILLED TUBE\u003c\/div\u003e\n\u003cdiv style=\"font-weight: bold; color: #0056b3; margin-bottom: 10px;\"\u003eLymphocyte Separation Tube (Prefilled)\u003c\/div\u003e\n\u003ca style=\"display: inline-block; width: 100%; text-align: center; padding: 10px 0; background: #0056b3; color: #fff; border-radius: 6px; text-decoration: none; font-size: 14px;\" href=\"https:\/\/biofargo.com\/products\/lymphocyte-separation-tube-for-human-peripheral-blood?variant=43275829674165\" target=\"_blank\"\u003e View Product \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"100mL\/bottle","offer_id":43275988598965,"sku":"7211011","price":140.0,"currency_code":"USD","in_stock":true},{"title":"250mL\/bottle","offer_id":43275988697269,"sku":"7211012","price":210.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_7ebc277e-4ab0-4d39-8237-78ead6d1b7d2.jpg?v=1776838756"},{"product_id":"animal-tissue-cell-total-rna-extraction-kit","title":"Animal Tissue Cell Total RNA Extraction Kit-Biosci™","description":"\u003ch2\u003eProduct Description:\u003c\/h2\u003e\n\u003cp\u003eAnimal Tissue Cell Total RNA Extraction Kit employs a rapid, phenol-free and chloroform-free RNA extraction technique for swift extraction of total RNA from easily lysable animal tissue and cell. The lysis buffer in this kit rapidly lyses cell and inactivates RNase, without the need for β-mercaptoethanol from conventional kits. The unique genomic DNA removal column guarantees effective removal of gDNA residue, eliminating the need for DNase digestion and allowing direct use of RNA for tests such as RT-PCR and qPCR. If downstream experiments are highly sensitive to trace DNA contamination, additional DNase treatment can be employed.\u003c\/p\u003e\n\u003cp\u003eThis kit extracts high-purity RNA fast and easily. Generally, the operation of a single sample can be completed within 30 minutes.\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\" style=\"margin-bottom: 16px; float: none;\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eStorage and Transportation:\u003c\/h2\u003e\n\u003cp\u003eStore at 2°C - 30°C, with a shelf life of 12 months.\u003cbr\u003eTransport at room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eScope ofApplication:\u003c\/h2\u003e\n\u003cp\u003eThe product can only be used for in vitro research\u003cbr\u003eand not for diagnostic purposes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Cultured Animal Cell Total RNA: (please read the precautions before the test).\u003c\/h2\u003e\n\u003cp\u003e1. Collect ≤ 1 × 10 7 cells by centrifuging at 300 × g for 5 min or 13,000 × g for 10 s, and remove supernatant.\u003c\/p\u003e\n\u003cp\u003e2. Add 350 μL (\u0026lt; 5 × 10 6 cells) or 600 μL (5 × 10 6 - 1 × 10 7 cells) of RLT lysis buffer, mix well by pipetting up and down, shake vigorously by hand for 20 s, and add all the lysis mixture to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Centrifuge at 13,000 × g for 1 min and retain the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Ensure that all liquid is filtered after centrifugation. Increase the centrifugal force and time if necessary.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Accurately measure the volume of the filtrate with a pipette, add an equal volume of 70% ethanol and mix well by pipetting up and down.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5. Immediately transfer the mixture to the adsorption column, which is put into the collection tube, centrifuge at 13,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Do not transfer more than 700 μL per time. If more than 700 μL of mixture needs to be transferred, do it in two separate transfers and centrifuge each time.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e6. Add 700 μL of rinsing solution 1, place at room temperature for 1 min, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e7. Add 500 μL of rinsing solution 2, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e8. Repeat Step 7.\u003c\/p\u003e\n\u003cp\u003e9. Put the adsorption column back into the collection tube and centrifuge at 13,000 × g for 2 minutes to completely remove residual ethanol via spin-drying.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The presence of residual ethanol can inhibit downstream reactions.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10. Put the adsorption column into an RNase-free 1.5 mL centrifuge tube, add 30 μL - 50 μL of eluent to the center of the adsorption column membrane, allow it to stand for 1 min, and centrifuge at 12,000 × g for 1min.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The yield can be improved by preheating the eluent in a water bath at 50°C - 70°C. If the expected RNA yield exceeds 30 μg, suck the eluent back into the adsorption column and repeat Step 10; or add another 30 μL - 50 μL of eluent and repeat Step 10, and mix the two eluents.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Animal Tissue Total RNA: (please read the precautions before the test)\u003c\/h2\u003e\n\u003cp\u003e1. Samples are first electrically homogenized or ground with liquid nitrogen.\u003c\/p\u003e\n\u003cp\u003e     a. \u003cstrong\u003eElectric homogenization:\u003c\/strong\u003e Quickly cut fresh tissues into small pieces with a scalpel, add 350 μL (\u0026lt; 20 mg of tissue) or 600 μL (20 mg - 30 mg of tissue) of RLT lysis buffer, and then electrically homogenize the mixture thoroughly for 20 - 40 s.\u003c\/p\u003e\n\u003cp\u003e     b. \u003cstrong\u003eGrinding with liquid nitrogen:\u003c\/strong\u003e Grind the tissue into fine powder with      liquid nitrogen, transfer an appropriate amount of fine tissue powder (20 mg\/30 mg) into an RNase-free 1.5 mL centrifuge tube containing 350 μL\/600 μL of RLT lysis buffer, and shake the tube vigorously by hand for 20 s to fully lyse.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOptional steps:\u003c\/strong\u003e Use a disposable 1 mL (with a 0.9 mm needle) syringe with a blunt needle to vigorously push and pull the plunger at least 10 times until a satisfactory homogenization effect is obtained, so as to shear DNAand reduce viscosity to prevent column plugging and improve yield.\u003c\/p\u003e\n\u003cp\u003e2. Centrifuge the homogenized mixture at 13,000 × g for 3 min, and transfer all the supernatant to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Follow Step 3 of extraction of cultured animal cell total RNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperation Steps:\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" data-mce-style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003ePrecautions:\u003c\/h2\u003e\n\u003cp\u003e1. Please read the operating instructions carefully before use.\u003c\/p\u003e\n\u003cp\u003e2. Before the first use, add absolute ethanol to the rinsing solution 2 reagent bottle (formal pack: 42 mL; trial pack: 4.2 mL); add absolute ethanol to the 70% ethanol bottle (formal pack: 21 mL; trial pack: 2.1 mL).\u003c\/p\u003e\n\u003cp\u003e3. Ensure that all solutions are clear before use. Low-temperature storage can cause precipitation and affect the use effect. The solution can be restored to clarity in a water bath at 37°C for several minutes.\u003c\/p\u003e\n\u003cp\u003e4. Perform all centrifugation at room temperature.\u003c\/p\u003e\n\u003cp\u003e5. The sample processing volume should not exceed the processing capacity of the genomic DNA removal column and RNA adsorption column to avoid DNA residue or yield reduction. The RNA\/DNA of various samples differs greatly, leading to a variation in the sample processing volume. When the experiment conditions are preliminarily explored, limit cell samples to 3 - 4 × 10 6 and tissue samples to 10 mg. Determine the processing volume for subsequent experiments based on the results.\u003c\/p\u003e\n\u003cp\u003e6. If residues are caused by excessive DNA content or rigorous fluorescent quantitative PCR is required, it is recommended to select intron-spanning primers or treat the RNAextract with RNase-free DNase.\u003c\/p\u003e\n\u003cp\u003e7. For the samples with excessively high DNAcontent, Step 6 can be performed as follows:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ea) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 30 s, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003eb) Add an appropriate amount of DNase working solution to the center of the adsorption column and place it at room temperature for 15 min.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ec) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 1 min, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e8. Ensure that equipment used in the experiment is RNase-free, including gloves, pipette tips, EP tubes and mortars.\u003c\/p\u003e\n\u003cp\u003e9. Bake the glassware at 150°C for 4 hours. Soak the plasticware in 0.5 M NaOH for 10 min, wash it thoroughly with water, and sterilize it to remove RNase.\u003c\/p\u003e\n\u003cp\u003e10. The product is only intended for scientific research conducted by professionals. For your safety and health, please wear lab coats and disposable gloves during the operation.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8034111_803411T_BioSci_Animal_Tissue_Cell_Total_RNA_Extraction_Kit.pdf?v=1700038025\" type=\"application\/pdf\" target=\"_blank\"\u003eAnimal Tissue Cell Total RNA Extraction Kit\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"50preps\/Kit","offer_id":43541462057141,"sku":"8034111","price":258.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8034111BioSci_AnimalTissueCellTotalRNAExtractionKit_1.png?v=1732865877"},{"product_id":"instant-tbs-t-buffer-biosci","title":"Instant TBS-T Buffer-SuperCulture™","description":"\u003ch2\u003eProduct Composition\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 71.0995%; height: 46px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 18px;\"\u003e8015131\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 18px;\"\u003e8015132\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 28px;\"\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003eInstant TBS-T Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; height: 28px;\"\u003e20 packs\/box\u003c\/td\u003e\n\u003ctd style=\"width: 33.0915%; text-align: center; height: 28px;\"\u003e100 packs\/box\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The pH value of 1 × solution is 7.4±0.2@25°C.\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage and Transportation Conditions\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature of 10-30°C, protected from light, with a shelf life of 36 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eInstant TBS-T Buffer is white to off-white instant granules, and each pack of this product can produce 1 L of 1 × TBS-T Buffer, which is easy to operate and use. The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salt and Tween-20. The concentration of sodium chloride, potassium chloride and Tris buffer salt in 1 × TBS-T Buffer is 137 mM, 2.7 mM and 25 mM respectively, and the content of Tween-20 is 0.05%.\u003cbr\u003eInstant TBS-T Buffer is widely used in biology, which is mainly used in immunohistochemistry, in situ hybridization, ELISA and western blot, and is also commonly used as wash buffer, as well as for antibody dilution and preparation of blocking buffer.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProcedure\u003c\/h2\u003e\n\u003cp\u003e1. Place a clean beaker, and add the entire contents of a newly opened pack of Instant TBS-T Buffer.\u003cbr\u003e2. Measure about 500 ml of pure water, slowly add it into the beaker, and gently stir with a stir bar until it is completely dissolved. (This step can also be performed with magnetic stirrer)\u003cbr\u003e3. Transfer the TBS-T solution in Step 2 into a constant volume container, and add water to dilute to 1 L, which is 1 × working solution.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eSchematic Diagram of Instant Buffer Preparation\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cimg style=\"float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206101453_1024x1024.png?v=1701828943\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\"\u003e\u003cstrong\u003eNote: Wash the container sufficiently with pure water before preparation, and clean it in time after preparation.\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ch2\u003e\u003cstrong\u003eDocument\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8015131_8015132_TBS-T_SuperDissol_Instant_TBS-T_Buffer.pdf?v=1701828598\" type=\"application\/pdf\" target=\"_blank\"\u003eInstant TBS-T Buffer-Biosci\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Dakewe","offers":[{"title":"20 packs\/box","offer_id":43380281475253,"sku":"8015131","price":65.0,"currency_code":"USD","in_stock":true},{"title":"100 packs\/box","offer_id":43380281508021,"sku":"8015132","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/80151318015132TBS-TSuperDissol_InstantTBS-TBuffer_1.png?v=1732875273"},{"product_id":"superculture-tm-l500-serum-free-medium-for-lymphocyte-gmp","title":"L500 Serum-Free Medium for Lymphocyte (GMP)-SuperCulture™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture™ L500 serum-free medium for lymphocyte is an upgraded medium developed by Dakewe based on the SuperCulture TM L100 serum-free medium for lymphocyte and applicable to in vitro culture of human lymphocytes. This product is a chemically defined medium, all the compositions of which are produced from cell culture grade materials. This product contains no heterogeneous animal component or serum or any other protein component except albumin, transferrin and insulin and can effectively avoid the influence of serum qualitative variation, serum components and exogenous components on experimental research and achieve the high-density culture of immunocytes such as human lymphocytes, cytokine induced killer cells, natural killer cells and dendritic cells.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHow to Use\u003c\/h2\u003e\n\u003cp\u003eThe SuperCulture TM L500 serum-free medium for lymphocyte supports the high-density suspension culture of lymphocytes. It supports the suspension culture of lymphocytes with the density of 5×10 5 ~5×10 6 cells\/mL in a static culture container. To ensure sufficient gas exchange of the culture system, the recommended depth of the medium is 1~1.5 cm. The medium also supports the high-density culture of lymphocytes in a bioreactor, and the optimization of the culture procedure should be determined by researchers based on experience.\u003c\/p\u003e\n\u003cp\u003eThe SuperCulture TM L500 serum-free medium for lymphocyte supports the serum-free culture of immunocytes, and the addition of autologous plasma, human AB serum, FBS or serum substitutes can significantly improve the cell viability and increase the cell proliferation rate. The SuperGrow TM cell culture supplemental mix is recommended to obtain the optimal culture result.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExample: Multiplication Culture of Cytokine Induced Killer Cells\u003c\/h2\u003e\n\u003cp\u003e1. Place the SuperCulture TM L500 serum-free medium for lymphocyte at room temperature for equilibrium.The following operations shall be in an aseptic condition:\u003cbr\u003e2. Separate lymphocytes of human peripheral blood in an aseptic condition.\u003cbr\u003e3. Inoculate PBMCs obtained by separation to a T75 or T175 cell culture bottle with the SuperCulture TM L500 serum-free medium for lymphocyte at a concentration of 2~2.5×10 6 cells\/mL, add recombinant human IFN-γ, etc. , and culture in a 5% CO 2 incubator at 37℃.\u003cbr\u003e4. After 24 hr, i.e., on the first day, add CD3 monoclonal antibody, recombinant human IL-2, etc. to stimulate growth and proliferation of cytokine induced killer cells.\u003cbr\u003e5. From the fourth day, sample every two days to calculate the cell concentration, supplement fresh SuperCulture TM L500 serum-free medium for lymphocyte based on the calculation result, and adjust the cell concentration to 1.5×10 6 cells\/mL.\u003cbr\u003e6. On the seventh day, change a bottle with a larger volume or transfer into a cell culture bag according to the volume of the cell culture suspension. The maximum culture volume of a T75 culture bottle is 40 mL, and the maximum culture volume of a T175 culture bottle is 200 mL. When the volume of the medium is more than 200 mL, transfer the cell culture suspension into a cell culture bag.\u003cbr\u003e7. Cell proliferation and cell surface marker detection.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreservation Method\u003c\/h2\u003e\n\u003cp\u003eKeep away from light at 2-8℃ , valid for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\nPay attention to operate in an aseptic condition.\u003cbr\u003eThis product is only for research.","brand":"Dakewe","offers":[{"title":"1000mL\/Bottle","offer_id":43540989477045,"sku":"6811021","price":212.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6111021L500.png?v=1732875849"},{"product_id":"supercut™-xhoⅰ-biosci","title":"XhoI-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…C↓T C G A G…3'\u003cbr\u003e3'…G A G C T↑C…5'\u003cbr\u003eIsoschizomers*: PaeR7I, TliI, BssHI, Sfr274I, SlaI, StrI\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20231206151502_160x160.png?v=1701846927\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e3×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ XhoⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ XhoⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™XhoⅠ.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ AvrII was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ XhoⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria- Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be\u003cbr\u003eexpressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ XhoⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH 2 O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eTo 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003eTo 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eTo 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently suck or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃ or add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eimpaired\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"5000 units","offer_id":43541335736501,"sku":"8052441","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052441SuperCut_XhoIFastRestrictionEndonucleasespng.png?v=1732886496"},{"product_id":"supercut™-ndeⅰ-biosci","title":"NdeⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...C A↓T A T G...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...G T A T ↑A C...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: FauNDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206161534_160x160.png?v=1701850606\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NdeⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ NdeⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ NdeⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NdeⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NdeⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NdeⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NdeⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™  NdeⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e2\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"2000 units","offer_id":43541352087733,"sku":"8052251","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_91317f02-dc20-4d8e-823b-55878e93d954.jpg?v=1753176201"},{"product_id":"supercut™-nsiⅰ","title":"NsiⅠ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'...A T G C A↓T...3'\u003cbr data-mce-fragment=\"1\"\u003e3'...T↑A C G T A...5'\u003cbr data-mce-fragment=\"1\"\u003eIsoschizomers*: EcoT22I, Mph1103I, Zsp2IDI\u003cbr data-mce-fragment=\"1\"\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e*Isoschizomers may have different methylation sensitivities.\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206165333_160x160.png?v=1701852836\" style=\"margin-bottom: 1px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 72px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 72px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e500 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\" data-mce-style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation- redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 10 units of SuperCut™ NsiⅠ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of λDNA (HindIII digest) and 1 μL of SuperCut™ NsiⅠ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ NsiⅠ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ NsiⅠ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ NsiⅠ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ NsiⅠ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1% white colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 90px;\" data-mce-style=\"border-collapse: collapse; width: 100%; height: 90px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ NsiⅠ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\" data-mce-style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\" data-mce-style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\" data-mce-style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\" data-mce-style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\" data-mce-style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\" data-mce-style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e14\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e3\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e0\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e9\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eNo effect\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"500 units","offer_id":43541364605109,"sku":"8052291","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052291SuperCut_NsiIpng.png?v=1732878695"},{"product_id":"supercut™-hindⅲ-biosci","title":"HindⅢ-Biosci-SuperCut™","description":"\u003ch2\u003eRestriction Enzyme Site\u003c\/h2\u003e\n\u003cp\u003e5'…A↓A G C T T…3'\u003cbr\u003e3'…T T C G A↑A…5'\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231206170842_160x160.png?v=1701853741\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage Condition\u003c\/h2\u003e\n\u003cp\u003eStorage at -25℃~-15℃ and validity for 24 months.\u003cbr\u003eShipped on Blue Ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 72px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eComponents\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ (10 units\/μL)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e2×5000 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6×1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e6× Gel Loading Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 18px; text-align: center;\"\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eActivity Definition\u003c\/h2\u003e\n\u003cp\u003eOne unit is defined as the amount of enzyme required to digest 1 μg of λDNA in 50 μL the reaction at the optimal reaction temperature in 60 minutes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eSuperCut™ Restriciton Enzymes are a series of genetically engineered restriction enzymes that can accurately complete DNA digestion in 5~15 minutes, suitable for rapid digestion of plasmid DNA, PCR products or genomic DNA.\u003cbr\u003eSuperCut™ Restriciton Enzymes has the following characteris-tics: digestion can be completed within 5~15 minutes; share a diges-tion buffer, which greatly simplifies the digestion reaction system; good enzyme activity redundancy for easy substrate overload or com-plex template digestion. In addition, dephosphorylation and ligation reagent of Biosci TM is 100% active in SuperCut™ Buffer, supporting one-tube reaction and improving the experience of \"digestion-ligation-redigestion\".\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eRecommended Reaction Conditions\u003c\/h2\u003e\n\u003cp\u003e1× SuperCut™ Buffer；\u003cbr\u003eIncubate at 37℃；\u003cbr\u003eRefer to \"Protocol for Fast DNA Digestion\" for reaction setup.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eHeat Inactivation\u003c\/h2\u003e\n\u003cp\u003e1.Incubation at 80℃ for 20 minutes.\u003cbr\u003e2.Add an appropriate amount of 6 × Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eQuality Control\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFunctional Test\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 10 units of SuperCut™ HindⅢ incubated for 15 minutes at 37℃ results in complete digestion as determined by agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eProlonged Incubation \/ Star Activity Assay\u003c\/strong\u003e\u003cbr\u003eA 20 μL reaction in SuperCut™ Buffer containing 1 μg of pUC19 DNA and 1 μL of SuperCut™ HindⅢ incubated for 3 hours at 37℃ results in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. Longer incubation may result in star activity.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Digestion-Ligation-Redigestion Test\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, the DNA was digested using 10 units of SuperCut™ HindⅢ, and then the digestion product was recovered. The DNA fragments can be religated using an appropriate amount of T4 DNA Ligase at 22℃. After the ligation product is recovered again, the ligation product can be recut using SuperCut™ HindⅢ.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNon-specific Endonuclease Activity\u003c\/strong\u003e\u003cbr\u003eAt the optimal reaction temperature, 10 units of SuperCut™ HindⅢ was incubated with a 20 μL reaction in SuperCut™ Buffer containing 1 μg of supercoiled plasmid DNA for 4 h, and the plasmid DNA was still supercoiled detected using agarose gel electrophoresis.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eBlue\/White Screening Assay\u003c\/strong\u003e\u003cbr\u003eAn appropriate vector containing lacZα gene is digested by 10 units of SuperCut™ HindⅢ. The digested product is ligated and transformed into E.coli competent cell. On Luria-Bertani culture plate with X-Gal, IPTG and appropriate antibiotic, the successfully ligated β-galactosidase gene can be expressed and gives rise to a blue colony, while an interrupted gene (i.e. degraded DNA end) gives rise to a white colony. SuperCut™ restriction enzymes must produce fewer than 1%\u003cbr data-mce-fragment=\"1\"\u003ewhite colonies.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eMethod of application\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Protocol for Fast DNA Digestion\u003c\/strong\u003e\u003cbr\u003e① Combine the following reaction components on ice in the order indicated：\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 90px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003ePlasmid DNA\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003ePCR product\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003eGenomic DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eDNA a\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e≤ 1 μg\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e≤0.2 μg\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e≤ 5 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ HindⅢ\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e10 units\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e30-50 units\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eSuperCut™ 10×Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003e2 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e3 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 34.8394%; height: 18px; text-align: center;\"\u003eddH₂O\u003c\/td\u003e\n\u003ctd style=\"width: 21.4263%; height: 18px; text-align: center;\"\u003eUp to 20 μL\u003c\/td\u003e\n\u003ctd style=\"width: 20.7962%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 30 μL\u003c\/td\u003e\n\u003ctd style=\"width: 22.9381%; height: 18px; text-align: center;\"\u003e\n\u003cspan data-mce-fragment=\"1\"\u003eUp to\u003c\/span\u003e 50 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003ea. DNA should be free of phenol, chloroform, ethanol, EDTA, detergents or high concentrations of salts, otherwise enzyme activity will be affected; Methylated DNA inhibits certain restriction enzyme digestion reactions.\u003c\/p\u003e\n\u003cp\u003e② Gently mix or flick the tube wall to mix well (never vortex), then centrifuge instantaneously to collect reaction solution；\u003cbr\u003e③ Incubate at 37℃ for 15 minutes (plasmid DNA) or for 15~30 minutes (PCR product) or for 30~60 minutes (genomic DNA)；\u003cbr\u003e④ Optional: Inactivate the enzyme by heating for 20 minutes at 80℃, and add an appropriate amount of 6× Gel Loading Buffer according to the reaction system to terminate the reaction.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Double and Multiple Digestion of DNA\u003c\/strong\u003e\u003cbr\u003e① Use 10 units of each enzyme and scale up the reaction conditions appropriately；\u003cbr\u003e② The combined volume of the enzymes in the reaction mixture should not exceed 1\/10 of the total reaction volume；\u003cbr\u003e③ If the enzymes require different reaction temperatures, start with the enzyme that requires a lower temperature, then add the second enzyme and incubate at the higher temperature.\u003cbr\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: If the total volume of reaction solution is larger than 20 μL, the incubation time should be increased appropriately, and water bath, metal bath, or sand bath should be used as much as possible.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003e\u003cstrong\u003e\u003cspan style=\"color: #000000;\"\u003eNumber of Recognition Sites in DNA\u003c\/span\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eλDNA\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003eΦX174\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epBR322\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC57\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003epUC18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003eSV40\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003eM13mp18\/19\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003eAdeno2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e0\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 12.5%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.6163%; text-align: center;\"\u003e6\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 13.9728%; text-align: center;\"\u003e1\u003cbr\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 11.9109%; text-align: center;\"\u003e12\u003cbr\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMethylation Effects on Digestion\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%; height: 36px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDam\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eDcm\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eCpG\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoKI\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eEcoBI\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e\u003cspan\u003eSome impaired\u003c\/span\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNo effect\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eSome impaired\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity in Different Buffers\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 36px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003eSuperCut™\u003cbr\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eThermo Scientific\u003cbr\u003eFastDigest Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eNEB\u003cbr\u003eCutSmart ® Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003eTakara\u003cbr\u003eQuickCut™ Buffer\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 16.7599%; height: 18px; text-align: center;\"\u003eActivity\u003c\/td\u003e\n\u003ctd style=\"width: 23.2401%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003ctd style=\"width: 20%; height: 18px; text-align: center;\"\u003e100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cspan style=\"color: #00aaff;\"\u003eNote: The activity data is from the standard reation test of Biosci\u003cspan data-mce-fragment=\"1\"\u003e™\u003c\/span\u003e Restriction Enzyme described above.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"10000 units","offer_id":43541383184565,"sku":"8052181","price":65.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8052181SuperCut_Hind_FastRestrictionEndonucleasespng.png?v=1732874002"},{"product_id":"residual-e-coli-dna-quantitation-kit-shentek®","title":"Residual E. coli DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E. coli DNA Quantitation Kit is designed for quantitative measurement of residual E. coli host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (qPCR) to enable rapid and specific quantitation at the femtogram level. It is suitable for a broad range of sample types from in-process samples to final products. The kit includes an E. coli Control DNA traceable to a primary DNA reference standard and a ready-to-use qPCR mix. For nucleic acid extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTotal reactions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as supplied with the kit).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n                        \u003ctd\u003eQuantitative PCR (absolute quantitation using standard curve).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDynamic range (standard curve)\u003c\/td\u003e\r\n                        \u003ctd\u003eStandard curve concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03 pg\/µL (recommended at least five concentration points).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n                        \u003ctd\u003eReported sensitivity: femtogram level (kit statement). Practical lowest standard used: 0.03 pg\/µL.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR reaction volume set to 30 µL (instrument setting described uses 30 µL).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/extension: 60 °C 01:00 (read fluorescence during this step).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended (ABI7500 SDS v1.4 example).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNegative and control acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NCS Ct should be larger than mean Ct of the lowest standard; NTC should be undetermined or Ct ≥ 35.00.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component labels).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus, Mx3000P, qTOWER3G, etc.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Enables quantitation at the femtogram level; lowest standard shown at 0.03 pg\/µL.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eTraceable control:\u003c\/strong\u003e Includes E. coli Control DNA traceable to a primary DNA reference standard for reliable standard curve construction.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Supplied E. coli qPCR MIX is provided in ready-to-use format to simplify reaction setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Validated conceptually for a variety of biopharmaceutical sample types from in-process to final products (recommended to perform method validation for specific matrices).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Protocol and plate layouts provided for a range of common real-time PCR instruments.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring of residual host DNA during purification workflows\u003c\/li\u003e\r\n                    \u003cli\u003eFinal product release testing (research use applications)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli DNA Control (NNA002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli qPCR MIX (NNC002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1 mL × 2 tubes (protect from light)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using a standard curve)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic procedures.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent contamination: use RNase\/DNase-free consumables, low-retention filter tips, UV-decontaminate surfaces and pipettes, and use separate areas for pre- and post-amplification steps.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents as indicated.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five standard concentrations for a reliable standard curve and perform method validation for your sample matrix.\u003c\/p\u003e\r\n                    \u003cp\u003e• Acceptable ERC recovery range: 50%–150%; verify NTC is undetermined or Ct ≥ 35 and NCS Ct is larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow instrument-specific software and parameter settings for data analysis (example settings provided for ABI 7500 SDS v1.4).\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research quantitation of residual E. coli host cell DNA in biopharmaceutical samples using qPCR (Research Use Only).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What are the standard curve concentrations supplied in the protocol?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes preparing standards at 3000, 300, 30, 3, 0.3 and 0.03 pg\/µL (use at least five concentrations for the standard curve).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%; NTC should be undetermined or Ct ≥ 35; NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I set up the qPCR cycling program?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended example: 95 °C for 10:00 (activation, 1 cycle); 95 °C for 0:15 (denaturation) and 60 °C for 1:00 (annealing\/extension with fluorescence read) for 40 cycles. Reaction volume example: 30 µL. Adjust program per instrument requirements.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186991784117,"sku":"SK030202E100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit.jpg?v=1742269328"},{"product_id":"serum-free-cell-freezing-medium-2-instructions-biosci™","title":"Serum-Free Cell Freezing Medium (2x) Instructions-BioSci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eSerum-Free Cell Freezing Medium (2×) is a chemically defined freezing medium developed by Dakewe on the basis of lymphocyte freezing kit, which has clear components and contains neither xenogeneic animal-derived components nor serum. On the basis of obtaining high cell viability and cell yield, this product can effectively avoid the change of serum quality and the impact of serum components and exogenous components on experimental research, and achieve excellent cell freezing and thawing effects.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eProduct Information\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\" data-mce-style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.9699%; text-align: center;\" data-mce-style=\"width: 30.9699%; text-align: center;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 38.6509%; text-align: center;\" data-mce-style=\"width: 38.6509%; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 30.3791%; text-align: center;\" data-mce-style=\"width: 30.3791%; text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 30.9699%; text-align: center;\" data-mce-style=\"width: 30.9699%; text-align: center;\"\u003e6032011\u003c\/td\u003e\n\u003ctd style=\"width: 38.6509%; text-align: center;\" data-mce-style=\"width: 38.6509%; text-align: center;\"\u003eSerum-Free Cell Freezing Medium (2×)\u003c\/td\u003e\n\u003ctd style=\"width: 30.3791%; text-align: center;\" data-mce-style=\"width: 30.3791%; text-align: center;\"\u003e100mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDirection for Use\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eReagents and instruments requiring self-preparation by the experimenter:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eCell medium (commonly used for culturing cells requiring freezing);\u003c\/li\u003e\n\u003cli\u003eClean bench;\u003c\/li\u003e\n\u003cli\u003eTemperature-controlled centrifuge, equipped with horizontal rotor with 15 mL and 50 mL centrifuge tubes, and recommended use of professional cell centrifuge; the sterile conical tube should be used as the centrifuge tube;\u003c\/li\u003e\n\u003cli\u003eP20, P200 and P1000 micropipettes and pipette tips, pipette aids and sterile serological pipettes;\u003c\/li\u003e\n\u003cli\u003eSterile 0.5 mL and 1.5 mL EP tubes;\u003c\/li\u003e\n\u003cli\u003eCell counter;\u003c\/li\u003e\n\u003cli\u003eFreezing container and sterile cryotube;\u003c\/li\u003e\n\u003cli\u003e-80℃ freezer;\u003c\/li\u003e\n\u003cli\u003eLiquid nitrogen tank;\u003c\/li\u003e\n\u003cli\u003eWater bath at 37°C.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell Freezing\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Mark the cryotubes to be used in detail. Place the cryotubes at -20℃ for thermal equilibration, and place the freezing container, cell medium and serum-free cell freezing medium at 4℃ for thermal equilibration.\u003c\/p\u003e\n\u003cp\u003e2. Count the cells to be frozen, and centrifuge 250 g at 4°C for 10 min.\u003c\/p\u003e\n\u003cp\u003e3. (Operation on ice) Discard the supernatant and resuspend with the cell medium equilibrated at 4℃ according to the results of cell count, so that the cell concentration is adjusted to twice the freezing concentration (e.g., if the cell concentration to be frozen is 1 × 10 7 cells\/mL, adjust the cell concentration to 2 × 10 7 cells\/mL with the medium), and then blow the cells off with the pipette evenly.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: The density of freezing cells recommended for this product is 5 × 10 5 - 2× 10 7 cells\/mL, and the appropriate density of freezing cells should be selected according to the nature of freezing cells and subsequent experimental arrangements during freezing.\u003c\/p\u003e\n\u003cp\u003e4. (Operation on ice) Slowly add an equal volume of serum-free cell freezing medium after placement in an ice bath dropwise, and mix gently.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: Be sure to add dropwise to avoid rapid changes in DMSO concentration in the medium.\u003c\/p\u003e\n\u003cp\u003e5. (Operation on ice) Dispense 1 mL of cell suspension per tube into freezing tubes equilibrated at -20°C, and then screw the cap tightly on the freezing tubes.\u003c\/p\u003e\n\u003cp\u003e6. Immediately place the freezing tube in the freezing container that has been equilibrated at 4℃, and then place the freezing container in a -80℃ freezer. Freezing tubes can also be placed in a programmable cooler to cool to -80℃ at a rate of 1℃\/min.\u003c\/p\u003e\n\u003cp\u003e7. Transfer freezing tubes stored at -80°C to liquid nitrogen 24 h later.\u003c\/p\u003e\n\u003cp\u003e8. Record the cell freezing location in the liquid nitrogen tank on the liquid nitrogen equipment management sheet in the laboratory.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCell Thawing\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e1. Perform thermal equilibration on cell medium at 4°C. Perform thermal equilibration on 50 mL and 15 mL centrifuge tubes at 4℃. Preadjust the water bath to 37℃.\u003c\/p\u003e\n\u003cp\u003e2. Remove the cryotubes from liquid nitrogen with forceps. If liquid nitrogen leaks into the freezing tube, unscrew the cap slightly to allow the vaporized liquid nitrogen to escape.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWarning\u003c\/strong\u003e: Some freezing tubes have been reported to explode upon thawing, and to eliminate this hazard, it is recommended that only firm polyethylene freezing tubes be used for liquid nitrogen preservation in experiments.\u003c\/p\u003e\n\u003cp\u003e3. Clamp the freezing tube with forceps and immerse it in the water bath at 37℃ (note that the pipette tip should not be immersed below the liquid level). Gently shake the freezing tube and pay attention to the thawing of freezing cells in the tube. When the ice nucleus becomes soybean-sized in the tube, take it out, wipe the tube body with alcohol cotton, and transfer it to a clean bench.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: This step is critical for cell viability. It is necessary to thaw as soon as possible to reduce the damage to the cells during thawing; in addition, the time of cells staying at high temperature should be reduced as much as possible to reduce the toxicity of DMSO to cells. Do not leave the freezing tube in the water bath.\u003c\/p\u003e\n\u003cp\u003e4. (Operation on ice) Open the cap of the freezing tube. Gently pipette the cell suspension (approximately 1 mL) from the freezing tube into a precooled 15 mL centrifuge tube. Slowly add 5 mL of cell medium (4℃), gently shake while adding, cover the tube cap upon completion of dropping, and mix well by gentle inversion.\u003c\/p\u003e\n\u003cp\u003e5. Continue to add fill up with the medium slowly (4℃) to 14 mL, screw the cap, and mix by gentle inversion.\u003c\/p\u003e\n\u003cp\u003e6. Centrifuge 250 g for 10 min at 4°C. Carefully aspirate the supernatant, and the supernatant is required to be aspirated as far as possible without touching the cells pelleted in the tube bottom. Disperse the cell mass by flicking the finger in the bottom of the centrifuge tube. Add 1 mL of medium and resuspend cells.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNotes\u003c\/strong\u003e: Aspirating the supernatant is more effective than decanting the supernatant, contributing to reduce the residual DMSO; finger flicking of the centrifuge tube can gently disperse the cell mass. Do not blow and aspire with a gun, otherwise it is harmful to the cells.\u003c\/p\u003e\n\u003cp\u003e7. (Optimization step, can be omitted) Cell clumping is caused by entanglement of DNA released from dead cells. 0.1 mL of DNase (1 mg\/mL) can be added and incubated at 37°C for 10 min.\u003c\/p\u003e\n\u003cp\u003e8. Take 50 μL of cell suspension and count trypan blue viable cells to calculate viability. Description: Viability = number of viable cells\/total number of cells × 100%. In the presence of trypan blue, viable cells are colorless and translucent, while dead cells are blue and dull.\u003c\/p\u003e\n\u003cp\u003e9. According to the counting results, fill up with cell medium to the desired cell concentration for subsequent assay.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eKeep away from light at 2~8℃, valid for 12 months.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid repeated freezing and thawing of this product, and pay attention to operate aseptically during use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eAssociated Product:\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 100%; height: 234px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003eCat. No.\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6911011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L100 Lymphocyte Serum-Free Medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6111021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L500 Lymphocyte Serum-Free Medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6811021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERCULTURE™ L500 Lymphocyte Serum-Free Medium\u003cbr\u003e(GMP)\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1000 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7922112\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman peripheral blood lymphocyte separation tube\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e15 mL × 20 vials, sieve\u003cbr\u003eplate tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7922021\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman peripheral blood lymphocyte separation tube\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e50 mL × 25 vials, sieve\u003cbr\u003eplate tube\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7912011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eDensity reagent\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e250 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e7111011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eHuman lymphocyte separation medium\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e100 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6122012\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERGROW™ Cell Culture Supplemental Mix\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e25 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6122011\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eSUPERGROW™ Cell Culture Supplemental Mix\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e250 mL\/bottle\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813521\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e3 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813522\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e2 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 24.1753%; height: 18px; text-align: center;\"\u003e6813523\u003c\/td\u003e\n\u003ctd style=\"width: 49.1382%; height: 18px; text-align: center;\"\u003eNatural Killer Cells Induction Culture Kit\u003c\/td\u003e\n\u003ctd style=\"width: 26.6864%; height: 18px; text-align: center;\"\u003e1 L\/Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6032011_BioSci_Serum-Free_Cell_Freezing_Medium_2x.pdf?v=1706174101\" type=\"application\/pdf\" target=\"_blank\"\u003eSerum-Free Cell Freezing Medium (2×) Instructions-BioSci™\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"100mL\/Bottle","offer_id":43547255013557,"sku":"6032011","price":232.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_499aa19b-0292-4cde-852d-f2195dcd3d11.jpg?v=1753176103"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle 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10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n                        \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n                        \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n                        \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n                        \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n                        \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWash steps\u003c\/td\u003e\r\n                        \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n                        \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n                        \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n                    \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n                    \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n                    \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-e-coli-dna-quantitation-kit-2g-shentek™","title":"Residual E. coli DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    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{\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli DNA Quantitation Kit (2G) is a duplex real‑time PCR (qPCR) assay designed to quantitate residual Escherichia coli host cell DNA in biopharmaceutical samples from in‑process material to final products. The kit provides high sensitivity (fg level), rapid and specific absolute quantitation using an internal positive control (IPC) included in the Primer \u0026amp; Probe mix to monitor PCR performance. The kit includes DNA control, qPCR master mix, DNA dilution buffer and Primer \u0026amp; Probe mix (with IPC). For extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Supplied reagents are sufficient for 100 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n                        \u003ctd\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (in‑process and final products)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd\u003eDuplex real‑time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay sensitivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReported sensitivity at the fg level (document states fg level performance)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003ePrepared serial dilutions: ST0 3000 pg\/µL (stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume (program)\u003c\/td\u003e\r\n                        \u003ctd\u003eThermal cycling reaction volume set to 30 µL (instrument setting referenced)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15, Annealing\/Extension 60 °C 1:00, 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCt thresholds \/ analysis settings\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct used. Thresholds: E.coli‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptance criteria\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eERC sample recovery: 50%–150%. NTC Ct ≥ 35.00. NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. IPC monitored in VIC channel.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd\u003eKit components can be stored under specified conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, LightCycler 480 (and other compatible real‑time PCR systems)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDuplex real‑time PCR:\u003c\/strong\u003e Simultaneous detection of E. coli target and IPC in the same reaction for reliable quantitation and internal reaction control.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual E. coli DNA down to the fg level using an absolute standard curve.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use components:\u003c\/strong\u003e Includes DNA control, qPCR Master Mix, Primer \u0026amp; Probe mix (with IPC) and DNA Dilution Buffer for streamlined workflow.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Built‑in criteria for NTC, NCS and ERC recovery (50%–150%) to support routine QC and method validation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad instrument support:\u003c\/strong\u003e Validated for common real‑time PCR instruments (ABI 7500, CFX96, LightCycler 480, SHENTEK‑96S).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical raw materials, intermediates and final products\u003c\/li\u003e\r\n                    \u003cli\u003eProcess development and in‑process monitoring\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing of biologics for host cell DNA\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and CRO testing workflows\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli DNA Control (NNA002) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Master MIX (NNB023) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli Primer \u0026amp; Probe MIX (incl. IPC) (NNC115) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDSs) before use; wear appropriate protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use DNA‑free consumables; perform UV decontamination of tabletop, pipettes and tubes for 30 minutes and disinfect surfaces with 75% ethanol to minimize contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and spin briefly. Protect light‑sensitive reagents (Master MIX and Primer \u0026amp; Probe MIX) from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at -20 °C. Unused DNA Dilution Buffer (DDB) may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include appropriate controls (standard curve, NTC, NCS, ERC) and run samples in replicates (recommended triplicates).\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow instrument‑specific settings when adapting the qPCR program; provided cycling parameters and analysis thresholds are validated on ABI 7500 SDS v1.4 and may need adjustment for other platforms.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity \/ limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports assay performance at the fg level (ultra‑sensitive); exact numeric LOD is not specified in the document and should be determined during method validation in your laboratory.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the supplied E. coli DNA Control to generate at least five standard points. Example dilutions in the guide: ST1 300 pg\/µL, ST2 30 pg\/µL, ST3 3 pg\/µL, ST4 0.3 pg\/µL, ST5 0.03 pg\/µL. Include standards in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC Ct should be ≥ 35.00. NCS Ct should be greater than the mean Ct of the lowest standard and show normal amplification in the VIC (IPC) channel.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186998108341,"sku":"1101107-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit_2G.jpg?v=1742277494"},{"product_id":"residual-cho-dna-quantitation-kit-2g-shentek™","title":"Residual CHO DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: 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color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit (2G) is intended to quantitate host-cell line residual DNA from CHO cell lines used in biopharmaceutical production. The kit uses duplex real-time PCR (qPCR) technology to perform rapid, specific and reliable quantitation down to the fg level. An Internal Positive Control (IPC) is included in the CHO Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit provides ready-to-use qPCR Master Mix, CHO Primer \u0026amp; Probe MIX with IPC, a quantified CHO DNA Control for standard curve generation, and DNA Dilution Buffer. For DNA extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Product No.: 1101100-1. Reagents for 100 reactions. For Research Use Only (RUO).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (label on kit: Reagents for 100 Reactions).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (serial dilutions)\u003c\/td\u003e\r\n                        \u003ctd\u003eST0 3000 pg\/μL (stock), ST1 300 pg\/μL, ST2 30 pg\/μL, ST3 3 pg\/μL, ST4 0.3 pg\/μL, ST5 0.03 pg\/μL, ST6 0.003 pg\/μL.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalytical range (based on standards)\u003c\/td\u003e\r\n                        \u003ctd\u003e3000 pg\/μL to 0.003 pg\/μL (nominal dynamic range as provided by standard series).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReported sensitivity \/ LOD (document statement)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eClaims quantitation at the fg level; lowest provided standard is 0.003 pg\/μL (3 fg\/μL).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\r\n                        \u003ctd\u003e20 μL qPCR MIX (15 μL qPCR Master MIX + 5 μL CHO Primer\u0026amp;Probe MIX) + 10 μL sample\/standard\/NTC\/NCS = 30 μL final reaction volume.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extension, fluorescence read).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection channels and dyes\u003c\/td\u003e\r\n                        \u003ctd\u003eCHO-DNA detector: FAM reporter; IPC detector: VIC reporter; Passive reference: ROX.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct selected. Threshold: CHO-DNA = 0.05, IPC = 0.1. Automatic baseline recommended. Instrument-specific settings may vary.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptability criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be ≥ 35.00 (or laboratory-specific threshold). NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. ERC recovery should be between 50% and 150%.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit components storage conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eAll listed components stored at -20 °C; protect light where indicated. Kit stable up to 24 months (check label expiration).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number \/ version \/ manufacturer\u003c\/td\u003e\r\n                        \u003ctd\u003eProduct No.: 1101100-1. User Guide Version: A\/1. Effective date: 01 Jul. 2024. Manufacturer: Biofargo, Inc.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR (qPCR):\u003c\/strong\u003e Simultaneous detection of CHO target sequence and an internal positive control (IPC) in the same reaction to monitor assay performance.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at the femtogram level; includes a dilution series down to 0.003 pg\/μL (3 fg\/μL) standard.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and CHO Primer \u0026amp; Probe MIX (with IPC) minimize assay setup time and reduce variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for common real-time PCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Roche LightCycler 480).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes quantified CHO DNA Control for standard curve generation, DNA Dilution Buffer (DDB), and IPC in the primer\/probe mix.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceuticals\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process testing and process development for biologics produced in CHO cells (research use)\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and assay development for host cell DNA monitoring\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control assays in research settings where CHO DNA detection is required\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eCHO Primer \u0026amp; Probe MIX (including IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent contamination: irradiate surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare at least five standard curve points; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n                    \u003cp\u003e• NTC Ct acceptance: ≥ 35.00 (or lab-specific threshold). NCS Ct should be greater than mean Ct of lowest standard and display normal VIC amplification.\u003c\/p\u003e\r\n                    \u003cp\u003e• ERC (extraction reference control) recovery must be between 50% and 150%.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation at the femtogram level. The lowest supplied standard is 0.003 pg\/μL (3 fg\/μL); actual limit of detection should be confirmed by laboratory validation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction (15 μL qPCR Master MIX + 5 μL CHO Primer \u0026amp; Probe MIX) and add 10 μL sample or standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which fluorescence channels are used for target and control?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCHO target is detected in the FAM channel; the internal positive control (IPC) is detected in the VIC channel; ROX is used as passive reference when applicable.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35.00 (or a lab-defined threshold). NCS Ct should be higher than the mean Ct of the lowest standard and show VIC amplification. ERC recovery should fall between 50% and 150%.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187351445685,"sku":"1101100-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit_2G.jpg?v=1742278220"},{"product_id":"residual-hek293-dna-quantitation-kit-3g-shentek™","title":"Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: 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color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual HEK293 DNA Quantitation Kit (3G) is designed to quantify residual HEK293 host cell DNA in biopharmaceutical samples at various stages (in‑process to final product). The kit uses duplex real‑time PCR (qPCR) for rapid, specific and reliable quantitation at the fg level. An Internal Positive Control (IPC) is included in the HEK293 Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit supports extraction workflows (refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit, Product No. 1104191) and is supplied as ready‑to‑use master mix and primer\/probe reagents for up to 100 reactions.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical samples\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay format\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDuplex real‑time PCR (qPCR) with target (FAM) and IPC (VIC) channels\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e20 μL qPCR MIX + 10 μL sample\/control = 30 μL total reaction volume\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReported sensitivity \/ LOD guidance\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit claims quantitation at the fg level. Lowest standard: 0.03 pg\/μL (30 fg\/μL). NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific limit based on validation).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eRecovery \/ Accuracy guidance\u003c\/td\u003e\r\n                        \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range: 50%–150%\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalysis settings (example for ABI7500 SDS v1.4)\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct. Thresholds: HEK293‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStability\u003c\/td\u003e\r\n                        \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, ABI 7500, BioRad CFX96, Roche LightCycler 480 and other real‑time PCR systems\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims quantitation at the femtogram (fg) level with standard curve down to 0.03 pg\/μL (30 fg\/μL).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (IPC) in the Primer \u0026amp; Probe MIX to monitor inhibition and reaction performance in each well.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use reagents:\u003c\/strong\u003e Supplied as qPCR Master MIX and premixed Primer \u0026amp; Probe, minimizing setup variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated on multiple real‑time PCR platforms (e.g., SHENTEK‑96S, ABI7500, CFX96, LightCycler 480).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharmaceutical testing:\u003c\/strong\u003e Optimized for residual host cell DNA measurement across in‑process and final product samples.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual HEK293 host cell DNA in upstream and downstream bioprocess samples\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control testing for biologics manufacturing\u003c\/li\u003e\r\n                    \u003cli\u003eIn‑process and final product residual DNA testing\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and routine monitoring of host cell DNA clearance\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 Primer \u0026amp; Probe MIX (Includes IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (gloves, lab coat, protective eyewear, mask) and work in nuclease‑free conditions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surfaces, pipettes and consumables by UV irradiation (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge. Protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform laboratory‑specific validation to establish LOD, LOQ and acceptance criteria.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure plate layout includes appropriate standards (at least five concentrations), NTC, NCS and ERC samples; run samples in triplicate when possible.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the claimed sensitivity of the kit and what are the lowest standard concentrations?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit claims quantitation at the femtogram (fg) level. The provided standard curve points include ST1 = 300 pg\/μL down to ST5 = 0.03 pg\/μL (30 fg\/μL). Laboratories should perform validation to determine actual LOD\/LOQ in their workflows.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I set up reaction volumes and plate layout?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction and add 10 μL of sample\/control to each well for a total reaction volume of 30 μL. Include at least five standard points, one NTC, one NCS and ERCs as described; triplicate wells are recommended for each sample.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended cycling conditions and analysis settings?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eExample conditions (ABI7500 SDS v1.4): Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 1:00 with fluorescence read during the 60 °C step. Suggested thresholds: HEK293‑DNA = 0.05; IPC = 0.1; use manual Ct and automatic baseline or adapt per instrument\/software.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should I use for ERC and controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific standard based on validation). The Ct of NCS should be larger than the mean Ct of the lowest standard and show IPC amplification in the VIC channel.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189158633653,"sku":"1101104-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Quantitation_Kit_3G.jpg?v=1742279848"},{"product_id":"residual-plasmid-dna-quantitation-kit-3g-shentek™","title":"Residual Plasmid DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n  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8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Plasmid DNA Quantitation Kit (3G) is a duplex real-time PCR (qPCR) kit designed to quantify residual plasmid DNA in gene therapy products (for example, plasmid DNA in lentiviral vector preparations used in CAR‑T manufacturing). The assay targets consensual plasmid sequences (replicon sequences of ColE1\/pMB1\/pBR322\/pUC) and uses a FAM-labeled target probe for quantitative detection and a VIC-labeled Internal Positive Control (IPC) included in the Primer\u0026amp;Probe MIX to monitor reaction performance. The kit provides reagents and a lyophilized plasmid linear DNA control for generation of a standard curve, enabling reliable quantitation down to the 10^2 copies\/µL level. The kit is supplied for 100 reactions and is intended for research use only. Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eApproximately 1×10^2 copies\/µL (sensitivity reported by manufacturer)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eQuantitative dynamic range \/ Standard curve\u003c\/td\u003e\r\n                        \u003ctd\u003eSerial dilution standard curve covers 4.97×10^8 to 4.97×10^1 copies\/µL (prepared from stock A down through ST5)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL total (20 µL qPCR MIX + 10 µL sample\/standard\/control)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Master MIX 15 µL + Plasmid Primer\u0026amp;Probe MIX (incl. IPC) 5 µL = 20 µL qPCR MIX\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extend; fluorescence read during this step)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eTarget probe: FAM (Plasmid-DNA); Internal Positive Control (IPC): VIC\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThreshold \/ analysis settings (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct threshold: Plasmid-DNA (FAM) = 0.05; IPC (VIC) = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NTC Ct should be ≥ (ST5 Ct + 2) or lab-specific; NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage \u0026amp; shelf life\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable for up to 24 months under recommended storage)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDuplex qPCR with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (VIC) within the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition or reaction failure.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Reported detection sensitivity at the 10^2 copies\/µL level for trace plasmid DNA quantitation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eConsensus plasmid sequence targeting:\u003c\/strong\u003e Assay targets consensual replicon sequences (ColE1\/pMB1\/pBR322\/pUC) to detect a broad range of common plasmid backbones.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents and controls:\u003c\/strong\u003e Includes lyophilized plasmid linear DNA control and pre-mixed Primer\u0026amp;Probe and Master MIX reagents to streamline setup.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480 and similar platforms).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eWorkflow for regulated testing environments:\u003c\/strong\u003e Provides recommended standard curve design, plate layouts, and acceptance criteria to support method validation and sample testing.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual plasmid DNA in gene therapy products\u003c\/li\u003e\r\n                    \u003cli\u003eMonitoring plasmid DNA residue in lentiviral vector preparations (CAR‑T production workflows)\u003c\/li\u003e\r\n                    \u003cli\u003eQuality control and release testing for biologics where plasmid backbone contamination must be measured\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and process development for plasmid removal\/clearance\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePlasmid linear DNA Control (NNA016), lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePlasmid Primer\u0026amp;Probe MIX (Incl. IPC) (NNC120), 500 µL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Master MIX (NNB023), 850 µL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and observe all handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves) when handling reagents and samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use DNase-free, low-retention tubes and tips to avoid contamination and sample loss.\u003c\/p\u003e\r\n                    \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments to minimize nucleic acid contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store all kit components at -20 °C. Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; clear cloudy DDB by warming to 37 °C if necessary.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include recommended controls (NTC, NCS, ERC, and standard curve points) and run samples in triplicate when possible.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow the provided plate layouts, thermal profile and analysis settings; adjust thresholds\/criteria only after laboratory validation.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect residual plasmid DNA down to approximately 1×10^2 copies\/µL. Actual limit of detection should be confirmed by the user's in-house validation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the lyophilized Plasmid linear DNA Control in 55 µL ddH2O, dilute to the stock concentration indicated on the tube, then perform serial 1:10 dilutions to generate standards A → ST5. Recommended standard points in the manual range from 4.97×10^8 to 4.97×10^1 copies\/µL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret ERC recovery and control criteria?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eERC (extraction reference control) recovery rates should fall between 50% and 150%. NCS Ct should be larger than the mean Ct of the lowest standard and show VIC amplification; NTC Ct should be ≥ ST5 Ct + 2 (or per your lab validation).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStore all kit components at -20 °C and protect light-sensitive reagents from light. Components are stable for up to 24 months when stored under recommended conditions.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189159813301,"sku":"1101111-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Plasmid_DNA_Quantitation_Kit_3G.jpg?v=1742288363"},{"product_id":"residual-vero-dna-size-analysis-kit-2g-shentek™","title":"Residual Vero DNA Size Analysis Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) based assay for rapid and specific quantitation of residual Vero (host cell) DNA fragments across multiple size ranges in biopharmaceutical samples (in-process to final product). The kit is designed to amplify four discrete fragment lengths (85 bp, 134 bp, 229 bp and 552 bp) and determine size distribution and quantity down to the femtogram (fg) level. Includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, DNA dilution buffer (DDB) and a Vero DNA control for generating standard curves. Reagents are provided for 4 × 100 reactions. For sample extraction and preparation refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 134 bp, 229 bp, 552 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReal-time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReported sensitivity \/ limit of detection\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDetects and quantifies Vero DNA down to the femtogram (fg) level (standard curve includes concentrations down to 0.03 pg\/µL)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (per kit dilution scheme)\u003c\/td\u003e\r\n                        \u003ctd\u003eST0 (prepared) 3000 pg\/µL; ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n                        \u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL template\/standard\/NTC (total 30 µL reaction volume). qPCR MIX composition per reaction: qPCR Reaction Buffer 15.9 µL; Vero Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL; total qPCR MIX 20 µL.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInternal control (IPC) criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eSample Ct(IPC) should be within ±1.0 Ct of NCS Ct(IPC). Significant increase indicates potential inhibition; evaluate recovery with ERC samples.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eNegative control (NTC) criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be ≥ 2 Ct higher than mean Ct of the lowest standard concentration, or laboratory-specific acceptance criteria established during validation.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months; check label expiration date.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQuantitation and size-distribution analysis of residual Vero DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMulti-fragment size analysis:\u003c\/strong\u003e Four specific primer\/probe assays amplify 85 bp, 134 bp, 229 bp and 552 bp fragments to determine size distribution of residual DNA.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual DNA at femtogram-level sensitivity using absolute quantitation and serial standard curves.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBuilt-in internal control:\u003c\/strong\u003e IPC mix included to monitor inhibition and reaction performance for each sample.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR reaction buffer and primer\/probe mixes minimize setup variability and speed workflow.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus) and adjustable to other qPCR instruments following instrument software guidance.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual Vero (host cell) DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n                    \u003cli\u003eAssessment of DNA fragment size distribution during downstream processing and in final product\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process testing and release testing in research and development (RUO) workflows\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and recovery studies for host cell DNA assays\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero Primer \u0026amp; Probe MIX-85 (NNC020) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero Primer \u0026amp; Probe MIX-134 (NNC021) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero Primer \u0026amp; Probe MIX-229 (NNC022) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero Primer \u0026amp; Probe MIX-552 (NNC023) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eVero DNA Control (NNA010) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProvided documentation\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions total) | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read MSDS and user guide before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent nucleic acid contamination: UV-decontaminate workspace, use DNase-free consumables, aliquot reagents and change tips between samples.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Store reagents as labeled; check expiration date on labels.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow instrument-specific settings and analysis parameters; adjust thresholds\/baselines per instrument\/software.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include appropriate controls: standards (ST1–ST5), NTC, NCS and IPC for each run.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103174.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103174.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed for femtogram-level sensitivity. The supplied standard curve includes concentrations down to 0.03 pg\/µL (ST5). Exact LOD\/LOQ should be established during laboratory validation.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500 (with ROX), Bio-Rad CFX96 and LineGene 9600plus. Other real-time PCR instruments are supported; follow instrument-specific setup and dye assignments.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted and what are acceptance criteria for controls?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eSet up an absolute quantitation standard curve (ST1–ST5). IPC Ct for samples should be within ±1.0 of the NCS IPC Ct; significant deviation indicates inhibition. NTC Ct should be at least 2 Ct higher than the mean Ct of the lowest standard (or per laboratory acceptance criteria).\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThaw Vero DNA Control and DDB on ice. Prepare ST0 at 3000 pg\/µL using DDB, then perform serial 1:10 dilutions to obtain ST1=300 pg\/µL, ST2=30 pg\/µL, ST3=3 pg\/µL, ST4=0.3 pg\/µL and ST5=0.03 pg\/µL. Include at least five standard points (suggested ST1–ST5) in triplicate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189155881141,"sku":"1103174","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Size_Analysis_Kit_2G.jpg?v=1742290678"},{"product_id":"residual-hek293-dna-size-analysis-kit-shentek™","title":"Residual HEK293 DNA Size Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HEK293 DNA Size Analysis Kit is designed to quantify residual HEK293 host cell DNA fragments of different sizes across biopharmaceutical sample types (in-process samples to final products) using real-time PCR (qPCR). The kit amplifies four fragment sizes (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer\/probe mixes for each fragment, internal positive control (IPC), DNA dilution buffer and a HEK293 DNA control for standard curve preparation. For extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n                        \u003ctd\u003eReal-time PCR (qPCR) with TaqMan-style primer \u0026amp; probe mixes and IPC\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eDesigned for quantitation at the femtogram (fg) level (lowest standard in kit workflow corresponds to 0.03 pg\/µL = 30 fg)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentrations (prepared from supplied HEK293 DNA Control)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock prior to dilution)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLinear \/ working range (as provided by recommended standards)\u003c\/td\u003e\r\n                        \u003ctd\u003eDemonstrated standard points from 0.03 pg\/µL to 300 pg\/µL (prepare standard curve using ST1–ST5); ST0 (3000 pg\/µL) used as stock\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 μL total reaction volume (20 μL qPCR master mix + 10 μL template)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR master mix composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; HEK293 Primer\u0026amp;Probe MIX (appropriate fragment) 2.8 μL; IPC MIX 1.3 μL; total 20 μL (then add 10 μL template)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/read: 72 °C 01:30; 40 cycles\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, FQD-96A and similar real-time PCR instruments (user must configure detectors\/channels accordingly)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIPC \/ quality control criteria\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eSample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. Ct of NTC and NCS should be larger than the mean Ct of the lowest concentration standard.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit stability \/ shelf life\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Simultaneous assays for four discrete fragment lengths (75, 122, 244, 562 bp) allow determination of residual HEK293 DNA size distribution.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify DNA down to the femtogram range (lowest recommended standard 0.03 pg\/µL = 30 fg).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated HEK293 Primer\u0026amp;Probe MIXes for each fragment size reduce setup time and minimize variability.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal process control (IPC):\u003c\/strong\u003e IPC MIX included to monitor inhibition and assay performance; acceptance criteria provided.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eFlexible instrument compatibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (instructions include detector\/channel setup and reference dye settings).\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA dilution buffer and HEK293 DNA control for standard curve preparation; refers to complementary sample prep kit for extraction.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical process and final product testing\u003c\/li\u003e\r\n                    \u003cli\u003eDetermination of host cell DNA fragment size distribution\u003c\/li\u003e\r\n                    \u003cli\u003eIn-process monitoring and process development for biologics produced in HEK293 cells\u003c\/li\u003e\r\n                    \u003cli\u003eRelease testing and quality control (research-use contexts)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-75 (NNC012), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-122 (NNC013), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-244 (NNC014), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-562 (NNC015), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eIPC MIX (NNC069), 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after thaw; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eHEK293 DNA Control (NNA005), 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare separate standard curves for each fragment-specific primer\/probe mix (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n                    \u003cp\u003e• Include at least five standard points (recommended ST1–ST5) and run samples in triplicate where possible.\u003c\/p\u003e\r\n                    \u003cp\u003e• qPCR run setup: reaction volume 30 μL (20 μL master mix + 10 μL template); thermal cycling and detector settings must be configured per instrument.\u003c\/p\u003e\r\n                    \u003cp\u003e• IPC acceptance: sample Ct-IPC should be within ±1.0 of NCS Ct-IPC — higher Ct-IPC indicates potential inhibition; consider recovery controls.\u003c\/p\u003e\r\n                    \u003cp\u003e• Negative controls (NTC) and NCS should show Ct values greater (later) than the mean Ct of the lowest standard point.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103176.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103176.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity \/ limit of detection (LOD) of this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to quantify HEK293 DNA at the femtogram level. The supplied standard curve includes points down to 0.03 pg\/µL (30 fg); actual LOD\/LOQ should be established during method validation in your laboratory.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which fragment sizes are measured and how are they assayed?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eFour fragment sizes are assayed separately using fragment-specific primer \u0026amp; probe mixes: 75 bp, 122 bp, 244 bp and 562 bp. Prepare four separate standard curves (one per fragment) and run each assay on the qPCR instrument.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which instruments are compatible?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems such as SHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, and FQD-96A. Users must configure detectors\/channels, passive reference dye (e.g., ROX if required), and cycling conditions according to their instrument.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibited?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (internal positive control). The sample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. A significantly higher Ct-IPC in the sample suggests inhibition; dilute the sample or perform recovery controls and re-test.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189170954421,"sku":"1103176","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Size_Analysis_Kit.jpg?v=1742291951"},{"product_id":"residual-host-cell-rna-sample-preparation-kit-shentek™","title":"Residual Host Cell RNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table 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10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature; 2-8 °C; -20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell RNA Sample Preparation Kit is a magnetic particle-based RNA extraction kit for isolation of residual host cell RNA from biological products (e.g., E. coli, 293T). The kit is compatible with manual workflows and automated extraction on the rHCDpurify system. Extracted RNA is suitable for downstream RNA quantitation (real-time qPCR) and other nucleic acid-based assays. The kit includes reagents for protein digestion (Proteinase K), binding, washing, magnetic particles, RNase inhibitor, glycogen carrier, and elution reagents. The kit is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eExtraction of residual host cell RNA from biological samples for downstream RNA quantitation (qPCR)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eExtraction method\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eMagnetic particle-based RNA binding, wash and elution\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n                        \u003ctd\u003eTypical: add 100 µL sample + 110 µL Proteinase K digestion solution; incubate 55 °C for 60 min; machine max digestion volume 300 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K dosing guidance\u003c\/td\u003e\r\n                        \u003ctd\u003eFor sample protein concentration 0–100 mg\/mL: 10 µL Proteinase K per sample; for 100–200 mg\/mL: 20 µL Proteinase K per sample (volumes mixed with Proteinase K Buffer as tabled)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding\u003c\/td\u003e\r\n                        \u003ctd\u003ePer sample: add 10 µL 5M NaCl + 220 µL working binding solution; add 200–400 µL isopropanol (≈1\/3–1\/2 total volume); add 10–15 µL magnetic particles; mix 3–5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWashing\u003c\/td\u003e\r\n                        \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL; air-dry magnetic particles 30 s–3 min to remove residual ethanol\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eAdd 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v); incubate 50 °C for 5 min with intermittent vortexing; separate beads and recover eluate\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n                        \u003ctd\u003eUse 10–15 µL per sample; vortex to re-suspend before use; recommended storage 2–8 °C\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWorking binding prep\u003c\/td\u003e\r\n                        \u003ctd\u003ePer sample working binding: 200 µL Binding solution + 10 µL diluted Precipitation solution I (1:99 in Dilution solution) + 10 µL Glycogen\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003epH requirement\u003c\/td\u003e\r\n                        \u003ctd\u003eIdeal sample pH 6.0–8.0; adjust with 1 M HCl or 1 M NaOH if pH \u0026lt;5.0 or \u0026gt;9.0\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures are stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatibility \/ equipment\u003c\/td\u003e\r\n                        \u003ctd\u003eCompatible with rHCDpurify automated system and manual extraction; requires real-time PCR system for downstream quantitation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic particle-based separation:\u003c\/strong\u003e Efficient magnetic bead capture of RNA for high recovery and clean eluates suitable for qPCR\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eAutomated and manual compatibility:\u003c\/strong\u003e Validated for use with rHCDpurify instrument and a manual workflow\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete kit for RNA extraction:\u003c\/strong\u003e Includes Proteinase K digestion reagents, binding\/wash\/elution buffers, magnetic particles, RNase inhibitor and glycogen carrier\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDesigned for residual host cell RNA:\u003c\/strong\u003e Optimized procedure for extraction of residual RNA from biological products and plasmid preparations\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eStable shelf life:\u003c\/strong\u003e Components stored at proper temperatures are stable up to 24 months\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of residual host cell RNA from biopharmaceutical samples (e.g., E. coli, 293T)\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for RNA quantitation by real-time qPCR\u003c\/li\u003e\r\n                    \u003cli\u003ePreparation of plasmid DNA samples with residual host RNA assessment\u003c\/li\u003e\r\n                    \u003cli\u003eRoutine QC workflows in research and development laboratories\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND016) 20 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND014) 30 mL × 1 (requires addition of 40 mL ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution solution (NND061) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution solution (NND062) 12 mL × 2\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5M NaCl (NND040) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase inhibitor (NND060) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePrecipitation solution I (NND003) 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based RNA extraction (manual or rHCDpurify automated)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only. Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n                    \u003cp\u003e• Wear appropriate PPE: gloves, protective eyewear, mask and lab clothing.\u003c\/p\u003e\r\n                    \u003cp\u003e• Add 40 mL ethanol to Wash buffer A before first use; prepare 70% ethanol Wash buffer B from Dilution solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare working elution solution by mixing RNase inhibitor : Elution solution = 1 : 200 (v:v) in RNase-free area.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare diluted Precipitation solution I at 1:99 (Precipitation solution I : Dilution solution) before preparing working binding solution.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ideal sample pH is 6.0–8.0. Adjust samples to neutral pH if outside range.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not over-dry magnetic particles; over-drying may reduce elution efficiency.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform downstream assays the same day as extraction to ensure accurate results.\u003c\/p\u003e\r\n                    \u003cp\u003e• When removing supernatant, do not disturb or aspirate magnetic bead pellet; use low-retention tips and centrifuge as instructed.\u003c\/p\u003e\r\n                    \u003cp\u003e• UV sterilize rHCDpurify instrument interior (suggested 15 minutes) before runs; allow \u0026gt;30 min between extractions if required.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201205.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201205.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What downstream assays are compatible with the extracted RNA?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted RNA is suitable for RNA quantitation by real-time qPCR and other nucleic-acid based assays. Use appropriate DNase treatment if required by your assay.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare Wash buffer A and B before first use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 40 mL of ethanol to Wash buffer A (NND014) before first use. Prepare Wash buffer B by mixing Dilution solution (NND062) with ethanol to make 70% ethanol.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended elution volumes and conditions?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v). Incubate at 50 °C for 5 minutes with intermittent vortexing, then separate beads and recover eluate.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this kit be used on an automated platform?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify automated extraction system (program rHCR-1201205) as well as manual extraction workflows.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45189191270581,"sku":"1201205","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_RNA_Sample_Preparation_Kit.jpg?v=1742293165"},{"product_id":"residual-e-coli-rna-quantitation-kit-2g-shentek™","title":"Residual E.coli RNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 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class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli RNA Quantitation Kit (2G) is designed for quantitation of host cell RNA from Escherichia coli in biopharmaceutical products. The kit employs a duplex reverse transcription quantitative PCR (qRT-PCR) format including a target detector (FAM) and an Internal Positive Control (IPC, VIC) in the E. coli RNA Primer \u0026amp; Probe MIX. The assay provides rapid, specific and reliable quantitation with femtogram-level sensitivity for the target gene. The kit includes One Step qPCR Buffer, One Step Enzyme MIX, Primer \u0026amp; Probe MIX (incl. IPC), E. coli RNA Control and RNase-free water, and is compatible with common real-time PCR instruments.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDetection chemistry\u003c\/td\u003e\r\n                        \u003ctd\u003eDuplex one-step reverse transcription quantitative PCR (one-step RT-qPCR) with FAM-labeled target and VIC-labeled IPC\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSensitivity \/ Limit of detection\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eTarget detectable at femtogram (fg) level (document states target gene can be determined at the femtogram level)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStandard curve concentration range\u003c\/td\u003e\r\n                        \u003ctd\u003eSerial standards prepared from 2000 pg\/µL (stock) producing working standards: 20, 2, 0.2, 0.02, 0.002 pg\/µL (ST1–ST5)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003eTotal reaction volume: 20 µL (15 µL qRT-PCR MIX + 5 µL sample\/standard\/controls)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqRT-PCR mix composition per well\u003c\/td\u003e\r\n                        \u003ctd\u003eOne Step qPCR Buffer 10 µL, One Step Enzyme MIX 1 µL, E. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) 4 µL — total 15 µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n                        \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 0:30 (1 cycle); 45 cycles of 95 °C 0:10 (denature) and 60 °C 0:40 (anneal\/extend, fluorescence read)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n                        \u003ctd\u003eManual Ct; Threshold 0.05; Automatic baseline; ROX as passive reference (for ABI 7500), set Standard Curve (Absolute Quantitation)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n                        \u003ctd\u003eNTC Ct should be at least 2 cycles higher than the lowest standard; ERC recovery acceptance 50%–150%; compare sample IPC Ct to NTC\/NCS IPC Ct to evaluate inhibition\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDNase digestion conditions (for plasmid samples)\u003c\/td\u003e\r\n                        \u003ctd\u003eIncubate samples with DNase I at 37 °C for 30–60 minutes; recommended DNase I final concentration 0.2–2 U\/µL depending on sample; ensure plasmid sample ≤100 ng\/µL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eDNase inactivation\u003c\/td\u003e\r\n                        \u003ctd\u003eOptions: (1) SHENTEK® Residual Host Cell RNA Sample Preparation Kit purification; (2) heat inactivation at 75 °C for 10 minutes; (3) other validated methods\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage \u0026amp; stability (kit)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at –20 °C; kit components stable up to 24 months (check label expiration)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eDuplex one‑step RT-qPCR:\u003c\/strong\u003e Combined reverse transcription and qPCR in a single reaction for speed and reduced handling\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Target detection at femtogram (fg) level enabling quantitation of trace residual E. coli RNA\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included in Primer \u0026amp; Probe MIX (VIC) to monitor PCR performance and potential inhibition\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated buffer, enzyme mix and primer\/probe mix to simplify setup and improve reproducibility\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflow compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, Bio-Rad CFX96)\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eValidated workflow for biopharmaceutical samples:\u003c\/strong\u003e Protocols and optional sample prep kit provided for plasmid DNA and protein expression products\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQuantitation of residual host cell RNA (HCR) from Escherichia coli in biopharmaceutical products\u003c\/li\u003e\r\n                    \u003cli\u003eResidual RNA testing in plasmid DNA preparations\u003c\/li\u003e\r\n                    \u003cli\u003eResidual RNA testing in protein expression products derived from E. coli\u003c\/li\u003e\r\n                    \u003cli\u003eMethod validation and in-process or release testing for downstream product quality control\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli RNA Control (NNA011) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eE. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) (NNC119) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 100 Reactions | Method: qRT-PCR (duplex one-step reverse transcription quantitative PCR)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n                    \u003cp\u003e• Protect light-sensitive components (buffer and enzyme mix) from light; store all kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice and mix gently; avoid repeated freeze-thaw cycles.\u003c\/p\u003e\r\n                    \u003cp\u003e• Work in RNase-free conditions: use RNase\/DNase-free consumables, decontaminate surfaces and irradiate equipment with UV for 30 minutes, disinfect with 75% ethanol.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform DNase I treatment to remove genomic DNA for plasmid-derived samples; incubate at 37 °C for 30–60 minutes and inactivate DNase I (e.g., 75 °C for 10 minutes or purification).\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure final plasmid concentration ≤100 ng\/µL when performing DNase digestion; use DNase I final concentration 0.2–2 U\/µL as appropriate.\u003c\/p\u003e\r\n                    \u003cp\u003e• Include controls: standards (ST1–ST5), NTC, NCS and ERC; ERC recovery should be within 50%–150% to accept sample results.\u003c\/p\u003e\r\n                    \u003cp\u003e• If sample IPC Ct is significantly higher than NTC\/NCS IPC Ct, sample inhibition is possible; interpret IPC as reference and consider sample recovery.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect the target gene at the femtogram (fg) level. Use the provided standard curve (typical working standards: 20 to 0.002 pg\/µL) to determine assay sensitivity in your system and matrix.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, Applied Biosystems 7500, Roche LightCycler 480 II and Bio-Rad CFX96. Program settings may require adjustment per instrument\/software.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples derived from plasmid DNA be prepared?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003ePlasmid-derived samples require DNase I digestion to remove genomic DNA (37 °C for 30–60 min). After digestion, inactivate or remove DNase I (e.g., heat 75 °C for 10 min or use the SHENTEK Residual Host Cell RNA Sample Preparation Kit) before qRT-PCR.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included and what are acceptance criteria?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a standard curve (ST1–ST5), no template control (NTC), negative control sample (NCS), and extraction recovery control (ERC). NTC Ct should be ≥2 cycles above the lowest standard; ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189194055861,"sku":"1201201-1","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E.coli_RNA_Quantitation_Kit_2G.jpg?v=1742293356"},{"product_id":"mycobacteria-dna-extraction-kit-mycoshentek™","title":"Mycobacteria DNA Extraction Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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{\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: 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color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eApproximately 50 minutes\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature or 2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycobacteria DNA Extraction Kit is designed for extraction of trace Mycobacteria DNA from a variety of biologic sample types. The kit supports direct extraction from sample volumes ≤400 μL or concentration of larger volumes by centrifugation prior to extraction. It is compatible with manual magnetic-particle based workflows and automated extraction using the rHCDpurify\/rDNApurify instrument. The kit workflow includes pretreatment, Proteinase K digestion, magnetic particle binding (with isopropanol), two wash steps (ethanol-based), and heat elution to produce PCR-ready DNA (minimum recommended eluate ≥40 μL). The kit is supplied for research use only and is intended for downstream molecular detection (e.g., real-time PCR) with MycoSHENTEK detection reagents or other PCR systems.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct number \/ kit size\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eProduct No.: 1503601; reagents for 50 extractions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eExtraction of Mycobacteria DNA from cell-free and cell culture samples for downstream molecular detection (research use only)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n                        \u003ctd\u003eRecommended ≤400 μL per sample; if \u0026gt;400 μL concentrate to ≈400 μL by centrifugation\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProteinase K\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 20 μL Proteinase K per sample during digestion\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLysis\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 100 μL Lysis buffer; incubate at 55 °C for 60 min (digestion)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eBinding\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 200 μL Binding solution + 200 μL isopropanol + 50 μL Magnetic particles per sample; shake 5 min for binding\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eWashes\u003c\/td\u003e\r\n                        \u003ctd\u003eWash 1: 700 μL Wash buffer A (ethanol added before use); Wash 2: 700 μL Wash buffer B (70% ethanol)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eElution\u003c\/td\u003e\r\n                        \u003ctd\u003eAdd 50 μL Elution buffer; incubate at 70 °C for 7 min; recover at least 40 μL eluate\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAutomated run time\u003c\/td\u003e\r\n                        \u003ctd\u003erHCDpurify \/ rDNApurify program: ~50 minutes (plate-based automated run)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCentrifugation speeds \/ times\u003c\/td\u003e\r\n                        \u003ctd\u003eCell-free sample concentration: 16000×g for 15 min; Cell pelleting: 72×g for 5 min; Debris precipitation: 450×g for 10–15 min; brief spins (3 s) used frequently\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eMagnetic particle separation time\u003c\/td\u003e\r\n                        \u003ctd\u003eSeparation typically 3–5 min\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAir-dry time (post-wash)\u003c\/td\u003e\r\n                        \u003ctd\u003e30 s to 3 min depending on environment; avoid over-drying\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eKit components storable under indicated conditions for up to 24 months\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eTrace DNA extraction:\u003c\/strong\u003e Optimized to extract low-abundance (trace) Mycobacteria DNA from various biologic matrices.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eMagnetic bead-based purification:\u003c\/strong\u003e Magnetic particle chemistry provides efficient capture and wash-based purification compatible with manual and automated workflows.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Validated for manual tube-based extraction and automated plate-based extraction using rHCDpurify \/ rDNApurify instruments.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePCR-ready eluate:\u003c\/strong\u003e Produces a concentrated eluate (recommended ≥40 μL) suitable for downstream real-time PCR detection.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eRoom-stable major reagents:\u003c\/strong\u003e Most reagents are stored at room temperature; selected reagents (e.g., cell lysis\/pretreatment\/magnetic particles) refrigerated as indicated.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eComplete workflow reagents included:\u003c\/strong\u003e Includes lysis, binding, wash, elution buffers, proteinase K, magnetic particles and pretreatment reagents for sample types.\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eExtraction of Mycobacteria DNA from cell-free biologics (e.g., serum, plasma, cell-free supernatants)\u003c\/li\u003e\r\n                    \u003cli\u003eExtraction of Mycobacteria DNA from cell culture samples and cell pellets\u003c\/li\u003e\r\n                    \u003cli\u003eSample preparation for downstream molecular assays including real-time PCR\/qPCR\u003c\/li\u003e\r\n                    \u003cli\u003eResearch applications and in-process quality control testing of biologics (Research Use Only)\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 1 bottle\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eWash buffer A (NND015) — add 20 mL anhydrous ethanol before use\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 15 mL × 1 bottle\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMB Cell lysis buffer (NND039)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eFollow label; 500 μL × 1 tube\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003ePretreatment buffer (NND041)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMagnetic particles (NND034)\u003c\/span\u003e\u003cstrong\u003eFollow label (equilibrate to room temp before use); 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eProteinase K (NND024)\u003c\/span\u003e\u003cstrong\u003eFollow label; 1 mL × 1 tube\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle-based DNA extraction (manual or automated)\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and use appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n                    \u003cp\u003e• Use separate, dedicated laboratory areas and equipment for reagent preparation, sample preparation, amplification, and post-amplification to minimize contamination.\u003c\/p\u003e\r\n                    \u003cp\u003e• Ensure ambient temperature ≥22 °C before starting experiments when recommended.\u003c\/p\u003e\r\n                    \u003cp\u003e• Balance and vortex magnetic particles before use; centrifuge tubes briefly (3 s) when instructed to collect liquid from caps\/walls.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prepare Wash buffer A by adding 20 mL anhydrous ethanol before first use; prepare Wash buffer B as 70% ethanol separately.\u003c\/p\u003e\r\n                    \u003cp\u003e• Do not over-dry the magnetic particle pellet after ethanol wash; over-drying may make resuspension difficult.\u003c\/p\u003e\r\n                    \u003cp\u003e• Perform mycobacterial DNA detection assays the same day as extraction to ensure accuracy.\u003c\/p\u003e\r\n                    \u003cp\u003e• For automated runs: perform UV sterilization of the instrument for at least 15 min and clean internal walls with 75% alcohol wipes before and after runs; minimum interval between extractions is 30 min.\u003c\/p\u003e\r\n                    \u003cp\u003e• After automated extraction, immediately transfer eluate to a new tube and ensure ≥40 μL is recovered for downstream assays.\u003c\/p\u003e\r\n                    \u003cp\u003e• Dispose of used tips and liquid waste according to laboratory biohazard and local regulations.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503601.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503601.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for cell-free samples (e.g., serum, plasma, supernatants) and cell culture samples (cell pellets\/resuspensions). Samples ≤400 μL can be processed directly; larger volumes should be concentrated to ≈400 μL before extraction.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended elution volume and expected yield for downstream PCR?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eStandard elution uses 50 μL Elution buffer with incubation at 70 °C for 7 minutes. Recover at least 40 μL eluate for downstream PCR\/qPCR applications.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I run the kit on an automated instrument?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with rHCDpurify \/ rDNApurify automated plate-based instruments. The automated program run time is approximately 50 minutes for a typical plate protocol.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should magnetic particles be handled before use?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eEquilibrate magnetic particles to room temperature for 10 minutes and vortex thoroughly to fully resuspend particles before aliquoting to samples to ensure consistent binding performance.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — For research purposes; not for diagnostic use\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45189176230069,"sku":"1503601","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Extraction_Kit.jpg?v=1742292295"},{"product_id":"mycobacteria-dna-detection-kit-mycoshentek™","title":"Mycobacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n    \n    border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n    font-size: 14px;\n    opacity: 0.9;\n    margin-bottom: 5px;\n}\n\n.shentek-header-value {\n    font-size: 18px;\n    font-weight: bold;\n}\n\n.shentek-section {\n    margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n    border-bottom: 2px solid #f0f4f8;\n    padding-bottom: 25px;\n    margin-bottom: 40px;\n}\n\n.shentek-section--card {\n    background: #fcfdfe;\n    border: 1px solid #d1d5db;\n    border-radius: 12px;\n    padding: 30px;\n}\n\n.shentek-title {\n    color: #0056b3;\n    font-size: 24px;\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-title-bar {\n    background: #0056b3;\n    width: 4px;\n    height: 24px;\n    margin-right: 12px;\n    display: inline-block;\n}\n\n.shentek-subtitle {\n    color: #2d3748;\n    font-size: 20px;\n    margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n    \n    padding-left: 15px;\n    margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n    margin-top: 0;\n    display: flex;\n    align-items: center;\n}\n\n.shentek-subtitle-icon {\n    margin-right: 12px;\n}\n\n.shentek-heading {\n    color: #2d3748;\n    font-size: 18px;\n    \n    padding-left: 12px;\n    margin-bottom: 15px;\n}\n\n.shentek-text {\n    font-size: 16px;\n    color: #4a5568;\n    margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n    overflow-x: auto;\n}\n\n.shentek-table {\n    width: 100%;\n    border-collapse: collapse;\n    border: 1px solid #e2e8f0;\n    font-size: 15px;\n}\n\n.shentek-table thead tr {\n    background-color: #0056b3;\n    color: #fff;\n    text-align: left;\n}\n\n.shentek-table th {\n    padding: 15px;\n}\n\n.shentek-table td {\n    padding: 12px 15px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n    font-weight: 600;\n    background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n    color: #c53030;\n    font-weight: 600;\n}\n\n.shentek-grid {\n    display: grid;\n    gap: 30px;\n}\n\n.shentek-grid--2col {\n    grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n    grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n    gap: 20px;\n}\n\n.shentek-grid--downloads {\n    grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n    gap: 15px;\n}\n\n.shentek-list {\n    padding-left: 20px;\n    font-size: 14.5px;\n    color: #4a5568;\n}\n\n.shentek-list li {\n    margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-box {\n    background: #f8fafc;\n    padding: 15px;\n    border-radius: 6px;\n    font-size: 13.5px;\n    color: #4a5568;\n    border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n    display: flex;\n    justify-content: space-between;\n    padding: 6px 0;\n    border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n    margin-top: 10px;\n    font-size: 12px;\n    color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n    \u003cdiv class=\"shentek-header-bar\"\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-header-item\"\u003e\r\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\r\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n        \u003ch2 class=\"shentek-title\"\u003e\r\n            \r\n            Product Description\r\n        \u003c\/h2\u003e\r\n        \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK® Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, and similar materials using real-time qPCR technology. The integrated system has been validated with a limit of detection of 10–100 CFU\/mL and can detect more than 100 different Mycobacteria species. The assay chemistry includes dUTP and is compatible with UNG (uracil-N-glycosylase) treatment to prevent carry-over contamination. An Internal Control (IC) is provided to monitor PCR inhibition and\/or extraction efficiency. The kit is supplied with positive and negative controls and is intended for research use only.\u003c\/p\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n            \u003ctable class=\"shentek-table\"\u003e\r\n                \u003cthead\u003e\r\n                    \u003ctr\u003e\r\n                        \u003cth\u003eParameter\u003c\/th\u003e\r\n                        \u003cth\u003eDetails\u003c\/th\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/thead\u003e\r\n                \u003ctbody\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n                        \u003ctd\u003e1503602\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eKit Format \/ Reactions\u003c\/td\u003e\r\n                        \u003ctd\u003eReagents for 50 Reactions\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eIntended use\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eQualitative detection of Mycobacteria contamination in cells and cell culture-derived biologicals\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n                        \u003ctd class=\"shentek-text-danger\"\u003eValidated at 10–100 CFU\/mL\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n                        \u003ctd\u003eValidated to detect \u0026gt;100 Mycobacteria species\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAssay type\u003c\/td\u003e\r\n                        \u003ctd\u003eReal-time qPCR (probe-based)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eTotal reaction volume\u003c\/td\u003e\r\n                        \u003ctd\u003e30 µL (10 µL qPCR MIX + 20 µL sample\/control)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n                        \u003ctd\u003eMB qPCR Reaction Buffer 8 µL; MB Primer \u0026amp; Probe MIX 1.5 µL; Internal Control (IC) 0.5 µL; total 10 µL. UNG optional (0.1 U per reaction).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n                        \u003ctd\u003eUNG 25 °C 10:00 (1 cycle); Activation 95 °C 10:00 (1 cycle); 45 cycles of Denaturation 95 °C 00:15, Annealing 60 °C 00:30, Extension 72 °C 01:30 (read fluorescence during extension).\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eControls\u003c\/td\u003e\r\n                        \u003ctd\u003ePositive Control (PC), No Template Control (NTC), Negative Control Sample (NCS), Internal Control (IC)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\r\n                        \u003ctd\u003eTypical interpretation: FAM positive cutoff Ct \u0026lt;40.00 (positive if at least one well Ct\u0026lt;40 and normal amplification); VIC (IC) expected Ct \u0026lt;35.00 for normal amplification; PC Ct \u0026lt;35.00. NTC FAM Ct ≥40.00 or no amplification.\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n                        \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, and other real-time PCR systems supporting probe detection\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                    \u003ctr\u003e\r\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n                        \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component storage)\u003c\/td\u003e\r\n                    \u003c\/tr\u003e\r\n                \u003c\/tbody\u003e\r\n            \u003c\/table\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Validated limit of detection down to 10–100 CFU\/mL for broad Mycobacteria detection.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed and validated to detect over 100 different Mycobacteria species.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCarry-over contamination control:\u003c\/strong\u003e Chemistry includes dUTP and is compatible with UNG treatment to prevent and eliminate carry-over contamination.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eInternal control (IC):\u003c\/strong\u003e IC monitors PCR inhibition and\/or extraction efficiency; can be added pre-extraction or to the PCR mix.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003ePre-validated workflow:\u003c\/strong\u003e Includes positive and negative controls and recommended plate layouts and acceptance criteria for straightforward result interpretation.\u003c\/li\u003e\r\n                    \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500).\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n                \u003cul class=\"shentek-list\"\u003e\r\n                    \u003cli\u003eQualitative detection of Mycobacteria contamination in cell lines\u003c\/li\u003e\r\n                    \u003cli\u003eScreening of cell culture-derived biologicals for Mycobacteria contamination\u003c\/li\u003e\r\n                    \u003cli\u003eRoutine mycoplasma\/contaminant monitoring workflows in research laboratories (Mycobacteria-specific)\u003c\/li\u003e\r\n                    \u003cli\u003eResearch studies requiring sensitive detection of Mycobacteria DNA\u003c\/li\u003e\r\n                \u003c\/ul\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box\"\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMB qPCR Reaction Buffer (NNB011)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light; 400 µL × 1\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMB Primer \u0026amp; Probe MIX (NNC035)\u003c\/span\u003e\u003cstrong\u003e2–8 °C until use or -20 °C; 75 µL × 1\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMB Internal Control (IC) (NNA037)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or -20 °C; 600 µL × 1\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eMB Positive Control (PC) (NNA041)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 µL × 1\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-row\"\u003e\r\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or room temperature per label; 1.5 mL × 1\u003c\/strong\u003e\r\n                    \u003c\/div\u003e\r\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv\u003e\r\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n                    \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n                    \u003cp\u003e• Prevent contamination: use UV decontamination (30 minutes) and 75% ethanol to clean surfaces and equipment before setup.\u003c\/p\u003e\r\n                    \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n                    \u003cp\u003e• Use UNG enzyme optionally to reduce carry-over contamination (check effective amount before use).\u003c\/p\u003e\r\n                    \u003cp\u003e• If VIC (IC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\r\n                    \u003cp\u003e• Follow plate layout and include PC, NTC and NCS as described; use duplicates for samples and controls.\u003c\/p\u003e\r\n                \u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \r\n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-card\"\u003e\r\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n        \u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503602.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503602.pdf\" target=\"_blank\"\u003e\r\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n            \u003c\/a\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003csection class=\"shentek-section\"\u003e\r\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n        \u003cdiv class=\"shentek-faq\"\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe integrated system has been validated with a limit of detection of approximately 10–100 CFU\/mL.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the kit detect multiple Mycobacteria species?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The assay has been validated to detect more than 100 different Mycobacteria species.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is an Internal Control provided and how should it be used?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAn Internal Control (IC) is provided. It can be added during sample extraction to monitor extraction efficiency or added to the PCR mix to monitor PCR inhibition. If IC was added during extraction, replace IC volume with DNA dilution buffer when preparing the qPCR mix.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-faq-item\"\u003e\r\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended instruments and reaction setup?\u003c\/div\u003e\r\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500). Prepare 30 µL reaction volumes (10 µL qPCR MIX + 20 µL sample\/control) and run the recommended cycling program with UNG treatment if used.\u003c\/div\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/section\u003e\r\n\r\n    \u003cdiv class=\"shentek-notice\"\u003e\r\n        \u003cdiv class=\"shentek-notice-header\"\u003e\r\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n            \u003c\/svg\u003e\r\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n        \u003c\/div\u003e\r\n        \u003cdiv class=\"shentek-notice-body\"\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n            \u003cdiv class=\"shentek-notice-item\"\u003e\r\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n                    official compliance testing.\u003c\/p\u003e\r\n            \u003c\/div\u003e\r\n        \u003c\/div\u003e\r\n    \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"Default Title","offer_id":43795617874101,"sku":"1503602","price":1525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Detection_Kit.jpg?v=1742292467"},{"product_id":"mycoplasma-dna-extraction-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    min-width: 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#64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n    \u003cdiv class=\"shentek-header-bar\"\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature \/ 2–8 °C \/ -20 °C (stable for 24 months)\u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n        \u003ch2 class=\"shentek-title\"\u003e\n            \n            Product Description\n        \u003c\/h2\u003e\n        \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) is a magnetic particle–based DNA extraction kit designed for isolation of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks and complex sample matrices (e.g., up to 10^7 cells culture, 5% human albumin solutions, high-concentration plasmids). The kit is supplied for 50 extractions and is intended to be used in an integrated workflow together with the MycoSHENTEK® Mycoplasma Detection Kit (2G). The method is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection. The kit supports manual extraction and automated extraction using the rHCDpurify system. The validated detection limit is 10 CFU\/mL. The protocol includes sample pretreatment, enzymatic digestion (Proteinase K), magnetic-particle binding, wash steps, and elution to produce purified DNA suitable for downstream real-time PCR assays.\u003c\/p\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\n            \u003ctable class=\"shentek-table\"\u003e\n                \u003cthead\u003e\n                    \u003ctr\u003e\n                        \u003cth\u003eParameter\u003c\/th\u003e\n                        \u003cth\u003eDetails\u003c\/th\u003e\n                    \u003c\/tr\u003e\n                \u003c\/thead\u003e\n                \u003ctbody\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eIntended use \/ scope\u003c\/td\u003e\n                        \u003ctd\u003eExtraction of trace mycoplasma DNA from cell banks, virus seed banks and complex matrices (cell cultures, 5% human albumin, high-concentration plasmids).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eValidated standards\u003c\/td\u003e\n                        \u003ctd\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated when used with MycoSHENTEK® Mycoplasma Detection Kit (2G)).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eSample input volume\u003c\/td\u003e\n                        \u003ctd\u003eUp to ~400 µL directly; larger volumes should be concentrated by centrifugation and reduced to ~400 µL prior to extraction.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eReactions per kit\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 50 extractions.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eTypical digestion\/incubation conditions\u003c\/td\u003e\n                        \u003ctd\u003eAdd 20 µL Proteinase K and 100 µL Lysis buffer; incubate 55°C for 60 min for most matrices; for 5% human albumin incubate at 25°C for 60 min.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eBinding step\u003c\/td\u003e\n                        \u003ctd\u003ePer sample add 200 µL Binding Buffer, 200 µL isopropanol and 30 µL Magnetic particles; vertical mixing ~5 min to bind nucleic acids.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eWash steps\u003c\/td\u003e\n                        \u003ctd\u003eTwo washes: 700 µL Wash buffer A then 700 µL Wash buffer B; remove residual ethanol and air-dry magnetic pellet briefly (30 s–3 min).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eElution\u003c\/td\u003e\n                        \u003ctd\u003eElute with 50 µL Elution buffer; incubate at 70°C for 7 min with intermittent vortexing; recover eluate (~40 µL+ recommended for downstream assay).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eAutomated run time (rHCDpurify)\u003c\/td\u003e\n                        \u003ctd\u003eApprox. 43 minutes per plate run (program: Myco-2G).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eStorage stability\u003c\/td\u003e\n                        \u003ctd\u003eKit components stable up to 24 months when stored under recommended conditions (see component list).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eCritical centrifugation speeds\u003c\/td\u003e\n                        \u003ctd\u003eDebris removal: 7,250×g (10 s); concentration and plasmid\/cell concentration steps: 18,000×g (30 min); microcentrifuge brief spins: 3–10 s for liquid collection.\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eControls\u003c\/td\u003e\n                        \u003ctd\u003eNegative control sample (NCS) procedure provided; Internal Control (IC) (2G) is used during extraction (IC is supplied in the companion detection kit).\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                \u003c\/tbody\u003e\n            \u003c\/table\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated detection limit of 10 CFU\/mL when used with MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eBroad matrix compatibility:\u003c\/strong\u003e Works with cell cultures (up to 10^7 cells), cell culture supernatants, 5% human albumin solutions, high-concentration plasmids and other complex matrices.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports both manual magnetic-particle extraction and automated extraction on the rHCDpurify system.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003ePharmacopeial validation:\u003c\/strong\u003e Validated in accordance with USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection workflows.\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eComplete extraction chemistry:\u003c\/strong\u003e Includes lysis, binding, wash and elution reagents plus magnetic particles and proteinase K for robust nucleic acid recovery from low-copy targets.\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003eMycoplasma testing of master cell banks (MCBs) and working cell banks (WCBs)\u003c\/li\u003e\n                    \u003cli\u003eMycoplasma testing of virus seed banks\u003c\/li\u003e\n                    \u003cli\u003eTesting of cell culture samples (up to 10^7 cells)\u003c\/li\u003e\n                    \u003cli\u003eTesting of cell culture supernatants\u003c\/li\u003e\n                    \u003cli\u003eTesting of samples containing 5% human albumin\u003c\/li\u003e\n                    \u003cli\u003eExtraction from high-concentration plasmid preparations prior to mycoplasma detection\u003c\/li\u003e\n                    \u003cli\u003eSample preparation prior to real-time PCR-based mycoplasma detection\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box\"\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (after adding 20 mL anhydrous ethanol prior to first use)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (heat at 37°C if cloudy)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003ePretreatment buffer (NND002) — 1.25 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eMagnetic particles (NND033) — 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003e5 M NaCl (NND040) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003ePrecipitation solution I (NND003) — 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature (dilute 1:99 in DNA Dilution Buffer prior to use)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003ePrecipitation solution II (NND004) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eProteinase K (NND023) — 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C \/ according to label (keep frozen until use)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based DNA extraction (magnetic bead chemistry) for downstream qPCR\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n                    \u003cp\u003e• Read MSDS and use appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\n                    \u003cp\u003e• Divide lab into separate areas (negative\/preparation, positive\/sample prep, amplification) and use dedicated equipment to prevent cross-contamination.\u003c\/p\u003e\n                    \u003cp\u003e• Ensure ambient temperature is not lower than 22°C before starting experiments (per manual recommendation).\u003c\/p\u003e\n                    \u003cp\u003e• Centrifuge tubes before opening to avoid splashing; handle tubes carefully to prevent contamination.\u003c\/p\u003e\n                    \u003cp\u003e• Change gloves frequently and use different gloves\/lab coats in separate areas.\u003c\/p\u003e\n                    \u003cp\u003e• Dispose of used tips and liquid waste according to local regulations; do not open post-PCR products in amplification area.\u003c\/p\u003e\n                    \u003cp\u003e• Avoid over-drying magnetic particle pellet during ethanol removal to prevent incomplete elution.\u003c\/p\u003e\n                    \u003cp\u003e• Perform DNA detection the same day as extraction to ensure accuracy.\u003c\/p\u003e\n                    \u003cp\u003e• For automated runs on rHCDpurify: UV sterilize the instrument for ≥15 minutes and clean inner walls with 75% ethanol wipes before and after runs; minimum interval between extractions should be 30 minutes.\u003c\/p\u003e\n                    \u003cp\u003e• When removing supernatants, avoid aspirating magnetic particles; use magnetic stand and allow full particle separation (3–5 min) before removing supernatant.\u003c\/p\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n        \u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509840.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509840.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-faq\"\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection when this extraction kit is used with the MycoSHENTEK® Mycoplasma Detection Kit (2G)?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe workflow is validated with a detection limit of 10 CFU\/mL when used with the MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I process sample volumes larger than 400 µL?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. For sample volumes \u0026gt;400 µL the protocol requires concentration by centrifugation to a final volume of approximately 400 µL before extraction.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which samples require a different digestion temperature?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eMost sample types are incubated with Proteinase K at 55°C for 60 minutes. Samples containing 5% human albumin and their controls should be incubated at 25°C for 60 minutes.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the kit compatible with automation?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with automated extraction using the rHCDpurify system (example program Myco-2G, ~43 minutes runtime).\u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003cdiv class=\"shentek-notice\"\u003e\n        \u003cdiv class=\"shentek-notice-header\"\u003e\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-notice-body\"\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection\u003c\/strong\u003e – This product is intended\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n                    official compliance testing.\u003c\/p\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45191334133941,"sku":"1509840","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Extraction_Kit_2G.jpg?v=1742362510"},{"product_id":"mycoplasma-dna-detection-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n    font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color: #2c3e50;\n    line-height: 1.7;\n    margin: 0 auto;\n    background: #fff;\n    padding: 10px;\n}\n\n.shentek-header-bar {\n    background-color: #0056b3;\n    border-radius: 8px;\n    padding: 20px;\n    display: flex;\n    flex-wrap: wrap;\n    justify-content: space-around;\n    align-items: center;\n    color: #ffffff;\n    margin-bottom: 30px;\n    box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n    text-align: center;\n    padding: 10px;\n    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color: #64748b;\n    font-style: italic;\n}\n\n.shentek-box--warning {\n    background: #fff5f5;\n    border: 1px solid #feb2b2;\n    color: #c53030;\n}\n\n.shentek-box--warning p {\n    margin: 0;\n}\n\n.shentek-box--warning p + p {\n    margin-top: 8px;\n}\n\n.shentek-card {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    padding: 20px;\n    text-align: center;\n    background: #fff;\n}\n\n.shentek-card-title {\n    font-size: 14px;\n    margin: 0 0 15px 0;\n    height: 40px;\n    color: #2d3748;\n}\n\n.shentek-btn {\n    display: block;\n    padding: 8px;\n    background: #0056b3;\n    color: #fff;\n    text-decoration: none;\n    border-radius: 4px;\n    font-size: 13px;\n    font-weight: 600;\n}\n\n.shentek-download-link {\n    display: flex;\n    align-items: center;\n    background: #fff;\n    padding: 12px 18px;\n    border-radius: 6px;\n    border: 1px solid #cbd5e1;\n    text-decoration: none;\n    color: #334155;\n    font-size: 14px;\n    font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n    color: #ef4444;\n    font-weight: bold;\n    margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n    margin-left: auto;\n    color: #94a3b8;\n}\n\n.shentek-faq {\n    border: 1px solid #e2e8f0;\n    border-radius: 8px;\n    overflow: hidden;\n}\n\n.shentek-faq-item {\n    padding: 15px 20px;\n    border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n    border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n    background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n    font-weight: bold;\n    color: #0056b3;\n    margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n    font-size: 14px;\n    color: #4a5568;\n}\n\n.shentek-notice {\n    background-color: #fffbeb;\n    border: 1px solid #fde68a;\n    border-radius: 8px;\n    padding: 20px;\n    margin-top: 30px;\n    margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n    display: flex;\n    align-items: center;\n    gap: 8px;\n    margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n    width: 20px;\n    height: 20px;\n    color: #d97706;\n    flex-shrink: 0;\n}\n\n.shentek-notice-title {\n    font-weight: bold;\n    color: #78350f;\n    margin: 0;\n    font-size: 16px;\n}\n\n.shentek-notice-body {\n    margin-left: 28px;\n}\n\n.shentek-notice-item {\n    display: flex;\n    align-items: flex-start;\n    gap: 8px;\n    margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n    margin-bottom: 0;\n}\n\n.shentek-notice-check {\n    color: #d97706;\n    font-weight: bold;\n    flex-shrink: 0;\n}\n\n.shentek-notice-text {\n    margin: 0;\n    font-size: 14px;\n    color: #374151;\n    line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n    \u003cdiv class=\"shentek-header-bar\"\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-header-item\"\u003e\n            \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n            \u003cdiv class=\"shentek-header-value\"\u003e-20°C (stable for 24 months)\u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\n    \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n        \u003ch2 class=\"shentek-title\"\u003e\n            \n            Product Description\n        \u003c\/h2\u003e\n        \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR-based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culture–derived products. The kit is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII, with a stated limit of detection of 10 CFU\/mL. The assay detects \u0026gt;200 species with high specificity and includes Internal Control (IC, 2G) to monitor PCR inhibition and optionally to evaluate extraction efficiency when added prior to extraction. The kit is supplied for 50 reactions and is intended for research use only (RUO).\u003c\/p\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-table-wrapper\"\u003e\n            \u003ctable class=\"shentek-table\"\u003e\n                \u003cthead\u003e\n                    \u003ctr\u003e\n                        \u003cth\u003eParameter\u003c\/th\u003e\n                        \u003cth\u003eDetails\u003c\/th\u003e\n                    \u003c\/tr\u003e\n                \u003c\/thead\u003e\n                \u003ctbody\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eAssay type\u003c\/td\u003e\n                        \u003ctd\u003eQualitative real-time PCR (qPCR) assay\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eSpecies coverage\u003c\/td\u003e\n                        \u003ctd\u003eDetects \u0026gt;200 species of Mycoplasma, Spiroplasma and Acholeplasma\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eControls included\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003eInternal Control (IC) (2G), Positive Control (PC) (2G)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eReaction format \u0026amp; volume\u003c\/td\u003e\n                        \u003ctd\u003e30 μL total reaction volume: 10 μL qPCR MIX + 20 μL sample (per well)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eqPCR Mix composition (per reaction)\u003c\/td\u003e\n                        \u003ctd\u003eMy qPCR Reaction Buffer (2G) 8 μL; My Primer \u0026amp; Probe MIX (2G) 1.5 μL; Internal Control (IC) (2G) 0.5 μL (total 10 μL)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n                        \u003ctd\u003eUNG 25°C 10:00 (1); Activation 95°C 10:00 (1); 45 cycles of: Denaturation 95°C 00:15, Annealing 62°C 00:30, Extension 72°C 01:30 (fluorescence read during extension)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eDetection channels \/ dyes\u003c\/td\u003e\n                        \u003ctd\u003eTarget: FAM; Internal Control: VIC; ROX optional as passive reference\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n                        \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, Roche LightCycler 480 and other real-time PCR systems\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eIntended sample types \/ matrices\u003c\/td\u003e\n                        \u003ctd\u003eMaster cell bank, working cell bank, virus seed stock, cell culture-derived products and similar matrices (validated across a variety of matrices)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n                        \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n                        \u003ctd\u003e-20°C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eReconstitution instructions (controls)\u003c\/td\u003e\n                        \u003ctd\u003eReconstitute lyophilized IC (2G) in 600 μL DNA Dilution Buffer; reconstitute lyophilized PC (2G) in 500 μL DNA Dilution Buffer\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                    \u003ctr\u003e\n                        \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\n                        \u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35; PC: FAM Ct \u0026lt; 35; Unknown positive: FAM Ct \u0026lt; 40 (≥1 well) with normal amplification; If VIC inhibited, repeat extraction or remove inhibitors\u003c\/td\u003e\n                    \u003c\/tr\u003e\n                \u003c\/tbody\u003e\n            \u003c\/table\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated LOD of 10 CFU\/mL allowing detection of low-level mycoplasma contamination\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed to detect more than 200 Mycoplasma, Spiroplasma and Acholeplasma species with high specificity\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition and extraction monitoring:\u003c\/strong\u003e Internal Control (IC, 2G) can be added to PCR or to the sample prior to extraction to monitor PCR inhibition and evaluate extraction efficiency\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeia standards:\u003c\/strong\u003e Validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Provided My Primer \u0026amp; Probe MIX (2G) simplifies assay setup; lyophilized positive and internal controls included\u003c\/li\u003e\n                    \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (SHENTEK-96S, CFX96, 7500, LightCycler 480, etc.)\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n                \u003cul class=\"shentek-list\"\u003e\n                    \u003cli\u003eQualitative mycoplasma testing of master cell banks and working cell banks\u003c\/li\u003e\n                    \u003cli\u003eScreening of virus seed stocks for mycoplasma contamination\u003c\/li\u003e\n                    \u003cli\u003eTesting of cell culture–derived products and biologics for mycoplasma\u003c\/li\u003e\n                    \u003cli\u003eRoutine cell culture contamination monitoring and QC in R\u0026amp;D and production (research use only)\u003c\/li\u003e\n                \u003c\/ul\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box\"\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eInternal Control (IC) (2G) (Part No. NNA035) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 600 μL DNA Dilution Buffer)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003ePositive Control (PC) (2G) (Part No. NNA039) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 500 μL DNA Dilution Buffer)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eMy qPCR Reaction Buffer (2G) (Part No. NNB004) 400 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C, protect from light\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eMy Primer \u0026amp; Probe MIX (2G) (Part No. NNC065) 75 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-row\"\u003e\n                        \u003cspan\u003eUser Guide \/ Instructions for use\u003c\/span\u003e\u003cstrong\u003eRoom temperature (follow handling instructions)\u003c\/strong\u003e\n                    \u003c\/div\u003e\n                    \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv\u003e\n                \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n                \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n                    \u003cp\u003e• For research use only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n                    \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n                    \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n                    \u003cp\u003e• Work in a clean area: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\n                    \u003cp\u003e• Thaw kit components at 2–8°C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n                    \u003cp\u003e• Protect My qPCR Reaction Buffer from light during storage and use.\u003c\/p\u003e\n                    \u003cp\u003e• If Internal Control (IC) was added during extraction, substitute equal volume of DNA Dilution Buffer in qPCR mix instead of IC.\u003c\/p\u003e\n                    \u003cp\u003e• Follow recommended plate layout and include controls (PC, NTC, NCS) in duplicate.\u003c\/p\u003e\n                    \u003cp\u003e• If VIC (IC) signal is inhibited (Ct ≥ threshold), repeat extraction or remove inhibitors and repeat assay.\u003c\/p\u003e\n                    \u003cp\u003e• Dispose of biological and chemical waste according to local regulations.\u003c\/p\u003e\n                \u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \n    \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-card\"\u003e\n                \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n                \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n            \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n        \u003c\/h3\u003e\n        \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509841.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n            \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509841.pdf\" target=\"_blank\"\u003e\n                \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n            \u003c\/a\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003csection class=\"shentek-section\"\u003e\n        \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n        \u003cdiv class=\"shentek-faq\"\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated with a limit of detection of 10 CFU\/mL.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the Internal Control (IC) be added before extraction?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eYes. IC (2G) may be added to the sample prior to extraction to evaluate extraction efficiency; if IC is added during extraction, omit IC when preparing the qPCR mix and add equal volume of DNA Dilution Buffer instead.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which qPCR instruments are compatible with this kit?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500 and Roche LightCycler 480. Use instrument software to set FAM for target and VIC for IC.\u003c\/div\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-faq-item\"\u003e\n                \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted (Ct thresholds)?\u003c\/div\u003e\n                \u003cdiv class=\"shentek-faq-answer\"\u003eAcceptance criteria: Positive control (PC) FAM Ct \u0026lt; 35; NTC FAM Ct ≥ 40 or no amplification; Unknown sample: FAM Ct \u0026lt; 40 (at least one well) with normal amplification = Positive. If IC (VIC) is inhibited (VIC Ct ≥ threshold or absent), repeat extraction or remove inhibitors and retest.\u003c\/div\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/section\u003e\n\n    \u003cdiv class=\"shentek-notice\"\u003e\n        \u003cdiv class=\"shentek-notice-header\"\u003e\n            \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n                \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n            \u003c\/svg\u003e\n            \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n        \u003c\/div\u003e\n        \u003cdiv class=\"shentek-notice-body\"\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n                    for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n            \u003c\/div\u003e\n            \u003cdiv class=\"shentek-notice-item\"\u003e\n                \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n                \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n                    official compliance testing.\u003c\/p\u003e\n            \u003c\/div\u003e\n        \u003c\/div\u003e\n    \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45191337345205,"sku":"1509841","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Detection_Kit_2G.jpg?v=1742362689"},{"product_id":"human-igg-elisa-kit-multi-sciences","title":"Human IgG ELISA Kit - Multi Sciences","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\" data-mce-style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr class=\"sample_type\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eSAMPLE TYPE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003eSerum, plasma, cell culture supernatant, and other biological samples\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"sample_volume\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eSAMPLE VOLUME\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e100 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"sensitivity\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eSENSITIVITY\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e0.18 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"range\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eRANGE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e3.13 ng\/mL – 200 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"assay_time\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eASSAY TIME\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e3.5 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"recovery\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eRECOVERY\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e93% – 100%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"average_recovery\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eAVERAGE RECOVERY\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e1.04\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"intra_precision\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eINTRA PRECISION\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e5.5% – 7.6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"inter-precision\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eINTER-PRECISION\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e5.6% – 8.1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"platform\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003ePLATFORM\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003eELISA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"plate\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003ePLATE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003eDetachable 96-well plate\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"size\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eSIZE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e96T\/48T\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"storage\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eSTORAGE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003eIf the reagent kit is unopened, it should be stored at 4℃. However, if it has been opened, the standard solution should be stored at -20℃, while the other components should be stored at 4℃.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"delivery\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eDELIVERY\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e4℃ blue ice transportation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"components\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eCOMPONENTS\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003e96-well polystyrene enzyme-linked immunosorbent assay (ELISA) plate coated with anti-Human IgG monoclonal antibody\u003cbr\u003eHuman IgG freeze-dried standard\u003cbr\u003eIgG detect Antibody\u003cbr\u003eHRP-labeled streptavidin\u003cbr\u003eAssay Buffer(10×)\u003cbr\u003eSubstrate TMB\u003cbr\u003eStop Solution\u003cbr\u003eWashing Buffer(20×)\u003cbr\u003eSealing Film\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"assay_principle\"\u003e\n\u003cth style=\"width: 15.9401%; text-align: center;\" data-mce-style=\"width: 15.9401%; text-align: center;\"\u003eASSAY PRINCIPLE\u003c\/th\u003e\n\u003ctd style=\"width: 83.9237%; text-align: center;\" data-mce-style=\"width: 83.9237%; text-align: center;\"\u003eThis kit utilizes the double antibody sandwich enzyme-linked immunosorbent assay (ELISA) detection technique.Specific anti-human IgG antibodies are precoated on a high-affinity ELISA plate. Standard samples and test samples are added to the wells of the ELISA plate. After incubation, IgG present in the samples binds to the solid-phase antibodies. After washing to remove unbound substances, detection antibodies labeled with horseradish peroxidase (HRP) are added and incubated. After washing, a colorimetric substrate, TMB, is added and the plate is incubated in the dark for color development. The intensity of the color reaction is directly proportional to the concentration of IgG in the samples.A stop solution is added to terminate the reaction, and the absorbance value is measured at a wavelength of 450 nm (with a reference wavelength range of 570-630 nm).\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK171.jpg?v=1716539414\" data-mce-src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EK171.jpg?v=1716539414\"\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eCitations\u003c\/h2\u003e\n\u003cdiv class=\"hd\"\u003e\u003ca href=\"https:\/\/www.multisciences.net\/citation\/a-metagenome-wide-association-study-of-the-gut-microbiota-in-recurrent-aphthous-ulcer-and-regulation-by-thalidomide\" class=\"catation-item\" data-mce-block=\"true\" target=\"_blank\"\u003e\n\u003cdiv class=\"hd\"\u003eA metagenome-wide association study of the gut microbiota in recurrent aphthous ulcer and regulation by thalidomide.\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003eFrontiers in Immunology\u003cspan\u003e   \u003c\/span\u003e\u003ctime\u003e2022-10-19\u003c\/time\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003ca href=\"https:\/\/www.multisciences.net\/citation\/reduced-energy-metabolism-impairs-t-cell-dependent-b-cell-responses-in-patients-with-advanced-hbv-related-cirrhosis\" class=\"catation-item\" data-mce-block=\"true\" target=\"_blank\"\u003e\n\u003cdiv class=\"hd\"\u003eReduced Energy Metabolism Impairs T Cell-Dependent B Cell Responses in Patients With Advanced HBV-Related Cirrhosis\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003eFrontiers in Immunology\u003cspan\u003e   \u003c\/span\u003e\u003ctime\u003e2021-06-23\u003c\/time\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"hd\"\u003e\u003ca href=\"https:\/\/www.multisciences.net\/citation\/development-of-a-mammalian-cell-based-zz-display-system-for-igg-quantification\" class=\"catation-item\" data-mce-block=\"true\" target=\"_blank\"\u003e\n\u003cdiv class=\"hd\"\u003eDevelopment of a mammalian cell-based ZZ display system for IgG quantification\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003eBMC BIOTECHNOLOGY  \u003cspan\u003e   \u003c\/span\u003e\u003ctime\u003e2023-07-28\u003c\/time\u003e\n\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003ca href=\"https:\/\/www.multisciences.net\/citation\/flower-like-wse2-used-as-bio-matrix-in-ultrasensitive-label-free-electrochemical-immunosensor-for-human-immunoglobulin-g-determination\" class=\"catation-item\" data-mce-block=\"true\" target=\"_blank\"\u003e\n\u003cdiv class=\"hd\"\u003eFlower-like WSe2 used as bio-matrix in ultrasensitive label-free electrochemical immunosensor for human immunoglobulin G determination\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003eANALYTICAL SCIENCES\u003cspan\u003e     \u003c\/span\u003e\u003ctime\u003e2023-05-25\u003c\/time\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/a\u003e\u003c\/div\u003e\n\u003cdiv class=\"bd\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca class=\"no-lightbox\" href=\"https:\/\/ecdn.liankebio.cn\/en\/datasheet\/elisa\/EK171.pdf\" target=\"_blank\" type=\"application\/pdf\"\u003e\u003cspan\u003eDatasheet\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca class=\"no-lightbox\" href=\"https:\/\/ecdn.liankebio.cn\/en\/sds\/elisa\/EK171.pdf\" target=\"_blank\" type=\"application\/pdf\"\u003e\u003cspan\u003eSDS File\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!----\u003e\u003ch2\u003eDocuments\u003c\/h2\u003e\r\n\u003cp\u003e\u003ca target=\"_blank\" type=\"application\/pdf\" href=\"https:\/\/files.biofargo.com\/multisciences\/pdf\/EK171-AP4.pdf\"\u003e\u003cstrong\u003eHuman IgG ELISA Kit - Multi Sciences User Manual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"multisciences","offers":[{"title":"48T","offer_id":44184355635381,"sku":"EK171-1","price":320.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":44184341151925,"sku":"EK171","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/ELISA-1.jpg?v=1732874787"},{"product_id":"recombinant-human-tgfb1-protein","title":"Recombinant Human TGFB1 Protein","description":"\u003cp\u003e\u003cspan\u003eDescription\u003cbr\u003e\u003cbr\u003eRecombinant Human TGFB1 Protein (Transforming Growth Factor Beta 1) is a bioactive cytokine known for its crucial role in regulating cell proliferation, differentiation, and immune responses. TGFB1 is initially synthesized as a proprotein that undergoes proteolytic cleavage to yield the mature, active TGF-beta-1 and the latency-associated peptide (LAP). This recombinant form includes the functional core sequence of the mature protein and is ideal for use in in vitro studies requiring biologically active TGF-beta-1.\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eProtein general information\u003c\/span\u003e\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"height: 155px; width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eProtein name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003etransforming growth factor beta 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 53px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAlternative protein names\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTransforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide, LAP, Transforming growth factor beta-1, TGF-beta-1]\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eGene name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTGFB1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAlternative gene name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTGFB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAccession\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eP01137\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eOrganism\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e\n\u003cem\u003eHomo sapiens\u003c\/em\u003e (Human)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 17px;\"\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eFull length\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e390 amino acids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Information\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eProduct name\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRecombinant Human TGFB1 Protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePredicted molecular mass\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003e55 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eExpression host\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHEK293\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePurification tag\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eMBP at N-terminus,6xHis at C-terminus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eForm\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eBuffer\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003e0.15 M Phosphate buffered saline, pH 7.4\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003eShipping, storage and shelf life\u003c\/td\u003e\n\u003ctd style=\"width: 49.9663%; height: 3px; text-align: center; vertical-align: middle;\"\u003e\n\u003cp\u003eShipped on dry ice. Avoid repeated freeze-thaw cycles.\u003c\/p\u003e\n\u003cp\u003eUpon receipt,\u003c\/p\u003e\n\u003cp\u003e* 2 days when stored at 2 to 8 °C after thawing\u003c\/p\u003e\n\u003cp\u003e* Up to 12 months when aliquoted and stored at -20 to -80 °C\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eCore sequence\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp class=\"col-4 pl10 ptb5\"\u003eCore sequence start: Asp281\u003cbr\u003eCore sequence stop: Ser390\u003c\/p\u003e\n\u003cp class=\"col-8 plr10 ptb5 wb f12\"\u003e......DTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS......\u003c\/p\u003e\n\u003cdiv class=\"col-8 plr10 ptb5 f12\" style=\"text-align: left;\"\u003e\n\u003cp\u003eNote:The core sequence of the recombinant protein is shown above. The N and C termini have shortened by 0-10 amino acids. The exact sequence is proprietary information powered by SIEVE®.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct data\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eSDS-PAGE\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/31ebc4370166f179d3bfdc3e4fceb5b0_600x600.jpg?v=1717572446\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003cspan\u003eThe purified product was run on 15% SDS-PAGE at reducing conditions. The gel was stained with Coomassie brilliant blue and destained.\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!----\u003e","brand":"Absea","offers":[{"title":"Frozen \/ 100ug","offer_id":44248006852789,"sku":"P00073QCSN-F100","price":124.0,"currency_code":"USD","in_stock":true},{"title":"Frozen \/ 1mg","offer_id":44248006820021,"sku":"P00073QCSN-F1","price":618.0,"currency_code":"USD","in_stock":true},{"title":"Freeze-dried \/ 100ug","offer_id":44248006918325,"sku":"P00073QCSN-FD100","price":161.0,"currency_code":"USD","in_stock":true},{"title":"Freeze-dried \/ 1mg","offer_id":44248006885557,"sku":"P00073QCSN-FD1","price":804.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/31ebc4370166f179d3bfdc3e4fceb5b0.jpg?v=1732881327"},{"product_id":"streptavidin-conjugated-goat-anti-rabbit-igg-sangon","title":"Streptavidin-conjugated Goat anti-rabbit IgG","description":"\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eImmunoglobulin G (IgG),is one of the most abundant proteins in serum with normal levels between 9.5-12.5 mg\/ml in adult blood. IgG is important for our defense against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e1\/500-1000 for ELISA 1\/500-1000 for Western blotting 1\/50-200 for Immunohistochemistry\/cytochemistry\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/D110076_EN_D.pdf?v=1718933538\" type=\"application\/pdf\" target=\"_blank\"\u003eStreptavidin-conjugated Goat anti-rabbit IgG\u003c\/a\u003e\u003c\/p\u003e\n\u003c!----\u003e","brand":"Sangon","offers":[{"title":"100 ul","offer_id":44323557507253,"sku":"D110076-0100","price":78.0,"currency_code":"USD","in_stock":true},{"title":"1 ML","offer_id":44323557474485,"sku":"D110076-0001","price":604.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/D11XXXX.png?v=1732883966"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00001_16a4ee67-000b-452c-9da8-e7374e4ef580.jpg?v=1776243781","url":"https:\/\/biofargo.com\/collections\/immunology-inflammation.oembed?page=20","provider":"Biofargo","version":"1.0","type":"link"}