{"title":"Host Cell Residue Testing","description":"","products":[{"product_id":"residual-e-coli-dna-quantitation-kit-shentek®","title":"Residual E. coli DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 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5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E. coli DNA Quantitation Kit is designed for quantitative measurement of residual E. coli host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (qPCR) to enable rapid and specific quantitation at the femtogram level. It is suitable for a broad range of sample types from in-process samples to final products. The kit includes an E. coli Control DNA traceable to a primary DNA reference standard and a ready-to-use qPCR mix. For nucleic acid extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as supplied with the kit).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (absolute quantitation using standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (standard curve)\u003c\/td\u003e\r\n \u003ctd\u003eStandard curve concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03 pg\/µL (recommended at least five concentration points).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eReported sensitivity: femtogram level (kit statement). Practical lowest standard used: 0.03 pg\/µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eqPCR reaction volume set to 30 µL (instrument setting described uses 30 µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/extension: 60 °C 01:00 (read fluorescence during this step).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended (ABI7500 SDS v1.4 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative and control acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NCS Ct should be larger than mean Ct of the lowest standard; NTC should be undetermined or Ct ≥ 35.00.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component labels).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus, Mx3000P, qTOWER3G, etc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Enables quantitation at the femtogram level; lowest standard shown at 0.03 pg\/µL.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTraceable control:\u003c\/strong\u003e Includes E. coli Control DNA traceable to a primary DNA reference standard for reliable standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Supplied E. coli qPCR MIX is provided in ready-to-use format to simplify reaction setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Validated conceptually for a variety of biopharmaceutical sample types from in-process to final products (recommended to perform method validation for specific matrices).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Protocol and plate layouts provided for a range of common real-time PCR instruments.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of residual host DNA during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing (research use applications)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli qPCR MIX (NNC002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1 mL × 2 tubes (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using a standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use RNase\/DNase-free consumables, low-retention filter tips, UV-decontaminate surfaces and pipettes, and use separate areas for pre- and post-amplification steps.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents as indicated.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations for a reliable standard curve and perform method validation for your sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery range: 50%–150%; verify NTC is undetermined or Ct ≥ 35 and NCS Ct is larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific software and parameter settings for data analysis (example settings provided for ABI 7500 SDS v1.4).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research quantitation of residual E. coli host cell DNA in biopharmaceutical samples using qPCR (Research Use Only).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What are the standard curve concentrations supplied in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes preparing standards at 3000, 300, 30, 3, 0.3 and 0.03 pg\/µL (use at least five concentrations for the standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%; NTC should be undetermined or Ct ≥ 35; NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I set up the qPCR cycling program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended example: 95 °C for 10:00 (activation, 1 cycle); 95 °C for 0:15 (denaturation) and 60 °C for 1:00 (annealing\/extension with fluorescence read) for 40 cycles. Reaction volume example: 30 µL. Adjust program per instrument requirements.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186991784117,"sku":"SK030202E100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit.jpg?v=1742269328"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-e-coli-dna-quantitation-kit-2g-shentek™","title":"Residual E. coli DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli DNA Quantitation Kit (2G) is a duplex real‑time PCR (qPCR) assay designed to quantitate residual Escherichia coli host cell DNA in biopharmaceutical samples from in‑process material to final products. The kit provides high sensitivity (fg level), rapid and specific absolute quantitation using an internal positive control (IPC) included in the Primer \u0026amp; Probe mix to monitor PCR performance. The kit includes DNA control, qPCR master mix, DNA dilution buffer and Primer \u0026amp; Probe mix (with IPC). For extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Supplied reagents are sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (in‑process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real‑time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReported sensitivity at the fg level (document states fg level performance)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared serial dilutions: ST0 3000 pg\/µL (stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume (program)\u003c\/td\u003e\r\n \u003ctd\u003eThermal cycling reaction volume set to 30 µL (instrument setting referenced)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15, Annealing\/Extension 60 °C 1:00, 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt thresholds \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct used. Thresholds: E.coli‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC sample recovery: 50%–150%. NTC Ct ≥ 35.00. NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. IPC monitored in VIC channel.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under specified conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, LightCycler 480 (and other compatible real‑time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real‑time PCR:\u003c\/strong\u003e Simultaneous detection of E. coli target and IPC in the same reaction for reliable quantitation and internal reaction control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual E. coli DNA down to the fg level using an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use components:\u003c\/strong\u003e Includes DNA control, qPCR Master Mix, Primer \u0026amp; Probe mix (with IPC) and DNA Dilution Buffer for streamlined workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Built‑in criteria for NTC, NCS and ERC recovery (50%–150%) to support routine QC and method validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument support:\u003c\/strong\u003e Validated for common real‑time PCR instruments (ABI 7500, CFX96, LightCycler 480, SHENTEK‑96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical raw materials, intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and in‑process monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of biologics for host cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and CRO testing workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli Primer \u0026amp; Probe MIX (incl. IPC) (NNC115) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDSs) before use; wear appropriate protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNA‑free consumables; perform UV decontamination of tabletop, pipettes and tubes for 30 minutes and disinfect surfaces with 75% ethanol to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and spin briefly. Protect light‑sensitive reagents (Master MIX and Primer \u0026amp; Probe MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C. Unused DNA Dilution Buffer (DDB) may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (standard curve, NTC, NCS, ERC) and run samples in replicates (recommended triplicates).\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument‑specific settings when adapting the qPCR program; provided cycling parameters and analysis thresholds are validated on ABI 7500 SDS v1.4 and may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity \/ limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports assay performance at the fg level (ultra‑sensitive); exact numeric LOD is not specified in the document and should be determined during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the supplied E. coli DNA Control to generate at least five standard points. Example dilutions in the guide: ST1 300 pg\/µL, ST2 30 pg\/µL, ST3 3 pg\/µL, ST4 0.3 pg\/µL, ST5 0.03 pg\/µL. Include standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC Ct should be ≥ 35.00. NCS Ct should be greater than the mean Ct of the lowest standard and show normal amplification in the VIC (IPC) channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186998108341,"sku":"1101107-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit_2G.jpg?v=1742277494"},{"product_id":"residual-cho-dna-quantitation-kit-2g-shentek™","title":"Residual CHO DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit (2G) is intended to quantitate host-cell line residual DNA from CHO cell lines used in biopharmaceutical production. The kit uses duplex real-time PCR (qPCR) technology to perform rapid, specific and reliable quantitation down to the fg level. An Internal Positive Control (IPC) is included in the CHO Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit provides ready-to-use qPCR Master Mix, CHO Primer \u0026amp; Probe MIX with IPC, a quantified CHO DNA Control for standard curve generation, and DNA Dilution Buffer. For DNA extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Product No.: 1101100-1. Reagents for 100 reactions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (label on kit: Reagents for 100 Reactions).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (serial dilutions)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL (stock), ST1 300 pg\/μL, ST2 30 pg\/μL, ST3 3 pg\/μL, ST4 0.3 pg\/μL, ST5 0.03 pg\/μL, ST6 0.003 pg\/μL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e3000 pg\/μL to 0.003 pg\/μL (nominal dynamic range as provided by standard series).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD (document statement)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims quantitation at the fg level; lowest provided standard is 0.003 pg\/μL (3 fg\/μL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX (15 μL qPCR Master MIX + 5 μL CHO Primer\u0026amp;Probe MIX) + 10 μL sample\/standard\/NTC\/NCS = 30 μL final reaction volume.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extension, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels and dyes\u003c\/td\u003e\r\n \u003ctd\u003eCHO-DNA detector: FAM reporter; IPC detector: VIC reporter; Passive reference: ROX.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected. Threshold: CHO-DNA = 0.05, IPC = 0.1. Automatic baseline recommended. Instrument-specific settings may vary.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptability criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35.00 (or laboratory-specific threshold). NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. ERC recovery should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit components storage conditions\u003c\/td\u003e\r\n \u003ctd\u003eAll listed components stored at -20 °C; protect light where indicated. Kit stable up to 24 months (check label expiration).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ version \/ manufacturer\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1101100-1. User Guide Version: A\/1. Effective date: 01 Jul. 2024. Manufacturer: Biofargo, Inc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR (qPCR):\u003c\/strong\u003e Simultaneous detection of CHO target sequence and an internal positive control (IPC) in the same reaction to monitor assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at the femtogram level; includes a dilution series down to 0.003 pg\/μL (3 fg\/μL) standard.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and CHO Primer \u0026amp; Probe MIX (with IPC) minimize assay setup time and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for common real-time PCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Roche LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes quantified CHO DNA Control for standard curve generation, DNA Dilution Buffer (DDB), and IPC in the primer\/probe mix.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceuticals\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and process development for biologics produced in CHO cells (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control assays in research settings where CHO DNA detection is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer \u0026amp; Probe MIX (including IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five standard curve points; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct acceptance: ≥ 35.00 (or lab-specific threshold). NCS Ct should be greater than mean Ct of lowest standard and display normal VIC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction reference control) recovery must be between 50% and 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation at the femtogram level. The lowest supplied standard is 0.003 pg\/μL (3 fg\/μL); actual limit of detection should be confirmed by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction (15 μL qPCR Master MIX + 5 μL CHO Primer \u0026amp; Probe MIX) and add 10 μL sample or standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which fluorescence channels are used for target and control?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCHO target is detected in the FAM channel; the internal positive control (IPC) is detected in the VIC channel; ROX is used as passive reference when applicable.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35.00 (or a lab-defined threshold). NCS Ct should be higher than the mean Ct of the lowest standard and show VIC amplification. ERC recovery should fall between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187351445685,"sku":"1101100-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit_2G.jpg?v=1742278220"},{"product_id":"residual-hek293-dna-quantitation-kit-3g-shentek™","title":"Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px 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#f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual HEK293 DNA Quantitation Kit (3G) is designed to quantify residual HEK293 host cell DNA in biopharmaceutical samples at various stages (in‑process to final product). The kit uses duplex real‑time PCR (qPCR) for rapid, specific and reliable quantitation at the fg level. An Internal Positive Control (IPC) is included in the HEK293 Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit supports extraction workflows (refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit, Product No. 1104191) and is supplied as ready‑to‑use master mix and primer\/probe reagents for up to 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDuplex real‑time PCR (qPCR) with target (FAM) and IPC (VIC) channels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX + 10 μL sample\/control = 30 μL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD guidance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit claims quantitation at the fg level. Lowest standard: 0.03 pg\/μL (30 fg\/μL). NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific limit based on validation).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery \/ Accuracy guidance\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (example for ABI7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct. Thresholds: HEK293‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, BioRad CFX96, Roche LightCycler 480 and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims quantitation at the femtogram (fg) level with standard curve down to 0.03 pg\/μL (30 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (IPC) in the Primer \u0026amp; Probe MIX to monitor inhibition and reaction performance in each well.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use reagents:\u003c\/strong\u003e Supplied as qPCR Master MIX and premixed Primer \u0026amp; Probe, minimizing setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated on multiple real‑time PCR platforms (e.g., SHENTEK‑96S, ABI7500, CFX96, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharmaceutical testing:\u003c\/strong\u003e Optimized for residual host cell DNA measurement across in‑process and final product samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in upstream and downstream bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn‑process and final product residual DNA testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer \u0026amp; Probe MIX (Includes IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, lab coat, protective eyewear, mask) and work in nuclease‑free conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and consumables by UV irradiation (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge. Protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Perform laboratory‑specific validation to establish LOD, LOQ and acceptance criteria.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure plate layout includes appropriate standards (at least five concentrations), NTC, NCS and ERC samples; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the claimed sensitivity of the kit and what are the lowest standard concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit claims quantitation at the femtogram (fg) level. The provided standard curve points include ST1 = 300 pg\/μL down to ST5 = 0.03 pg\/μL (30 fg\/μL). Laboratories should perform validation to determine actual LOD\/LOQ in their workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I set up reaction volumes and plate layout?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction and add 10 μL of sample\/control to each well for a total reaction volume of 30 μL. Include at least five standard points, one NTC, one NCS and ERCs as described; triplicate wells are recommended for each sample.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended cycling conditions and analysis settings?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample conditions (ABI7500 SDS v1.4): Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 1:00 with fluorescence read during the 60 °C step. Suggested thresholds: HEK293‑DNA = 0.05; IPC = 0.1; use manual Ct and automatic baseline or adapt per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should I use for ERC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific standard based on validation). The Ct of NCS should be larger than the mean Ct of the lowest standard and show IPC amplification in the VIC channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189158633653,"sku":"1101104-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Quantitation_Kit_3G.jpg?v=1742279848"},{"product_id":"residual-plasmid-dna-quantitation-kit-3g-shentek™","title":"Residual Plasmid DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Plasmid DNA Quantitation Kit (3G) is a duplex real-time PCR (qPCR) kit designed to quantify residual plasmid DNA in gene therapy products (for example, plasmid DNA in lentiviral vector preparations used in CAR‑T manufacturing). The assay targets consensual plasmid sequences (replicon sequences of ColE1\/pMB1\/pBR322\/pUC) and uses a FAM-labeled target probe for quantitative detection and a VIC-labeled Internal Positive Control (IPC) included in the Primer\u0026amp;Probe MIX to monitor reaction performance. The kit provides reagents and a lyophilized plasmid linear DNA control for generation of a standard curve, enabling reliable quantitation down to the 10^2 copies\/µL level. The kit is supplied for 100 reactions and is intended for research use only. Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 1×10^2 copies\/µL (sensitivity reported by manufacturer)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitative dynamic range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd\u003eSerial dilution standard curve covers 4.97×10^8 to 4.97×10^1 copies\/µL (prepared from stock A down through ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total (20 µL qPCR MIX + 10 µL sample\/standard\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Master MIX 15 µL + Plasmid Primer\u0026amp;Probe MIX (incl. IPC) 5 µL = 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extend; fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget probe: FAM (Plasmid-DNA); Internal Positive Control (IPC): VIC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold: Plasmid-DNA (FAM) = 0.05; IPC (VIC) = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NTC Ct should be ≥ (ST5 Ct + 2) or lab-specific; NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable for up to 24 months under recommended storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex qPCR with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (VIC) within the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition or reaction failure.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Reported detection sensitivity at the 10^2 copies\/µL level for trace plasmid DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConsensus plasmid sequence targeting:\u003c\/strong\u003e Assay targets consensual replicon sequences (ColE1\/pMB1\/pBR322\/pUC) to detect a broad range of common plasmid backbones.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents and controls:\u003c\/strong\u003e Includes lyophilized plasmid linear DNA control and pre-mixed Primer\u0026amp;Probe and Master MIX reagents to streamline setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480 and similar platforms).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow for regulated testing environments:\u003c\/strong\u003e Provides recommended standard curve design, plate layouts, and acceptance criteria to support method validation and sample testing.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual plasmid DNA in gene therapy products\u003c\/li\u003e\r\n \u003cli\u003eMonitoring plasmid DNA residue in lentiviral vector preparations (CAR‑T production workflows)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for biologics where plasmid backbone contamination must be measured\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for plasmid removal\/clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid linear DNA Control (NNA016), lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid Primer\u0026amp;Probe MIX (Incl. IPC) (NNC120), 500 µL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023), 850 µL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and observe all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves) when handling reagents and samples.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention tubes and tips to avoid contamination and sample loss.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments to minimize nucleic acid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store all kit components at -20 °C. Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; clear cloudy DDB by warming to 37 °C if necessary.\u003c\/p\u003e\r\n \u003cp\u003e• Include recommended controls (NTC, NCS, ERC, and standard curve points) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the provided plate layouts, thermal profile and analysis settings; adjust thresholds\/criteria only after laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect residual plasmid DNA down to approximately 1×10^2 copies\/µL. Actual limit of detection should be confirmed by the user's in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the lyophilized Plasmid linear DNA Control in 55 µL ddH2O, dilute to the stock concentration indicated on the tube, then perform serial 1:10 dilutions to generate standards A → ST5. Recommended standard points in the manual range from 4.97×10^8 to 4.97×10^1 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret ERC recovery and control criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC (extraction reference control) recovery rates should fall between 50% and 150%. NCS Ct should be larger than the mean Ct of the lowest standard and show VIC amplification; NTC Ct should be ≥ ST5 Ct + 2 (or per your lab validation).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore all kit components at -20 °C and protect light-sensitive reagents from light. Components are stable for up to 24 months when stored under recommended conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189159813301,"sku":"1101111-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Plasmid_DNA_Quantitation_Kit_3G.jpg?v=1742288363"},{"product_id":"residual-vero-dna-size-analysis-kit-2g-shentek™","title":"Residual Vero DNA Size Analysis Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) based assay for rapid and specific quantitation of residual Vero (host cell) DNA fragments across multiple size ranges in biopharmaceutical samples (in-process to final product). The kit is designed to amplify four discrete fragment lengths (85 bp, 134 bp, 229 bp and 552 bp) and determine size distribution and quantity down to the femtogram (fg) level. Includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, DNA dilution buffer (DDB) and a Vero DNA control for generating standard curves. Reagents are provided for 4 × 100 reactions. For sample extraction and preparation refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 134 bp, 229 bp, 552 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReal-time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects and quantifies Vero DNA down to the femtogram (fg) level (standard curve includes concentrations down to 0.03 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit dilution scheme)\u003c\/td\u003e\r\n \u003ctd\u003eST0 (prepared) 3000 pg\/µL; ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL template\/standard\/NTC (total 30 µL reaction volume). qPCR MIX composition per reaction: qPCR Reaction Buffer 15.9 µL; Vero Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL; total qPCR MIX 20 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control (IPC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eSample Ct(IPC) should be within ±1.0 Ct of NCS Ct(IPC). Significant increase indicates potential inhibition; evaluate recovery with ERC samples.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 2 Ct higher than mean Ct of the lowest standard concentration, or laboratory-specific acceptance criteria established during validation.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months; check label expiration date.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation and size-distribution analysis of residual Vero DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-fragment size analysis:\u003c\/strong\u003e Four specific primer\/probe assays amplify 85 bp, 134 bp, 229 bp and 552 bp fragments to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual DNA at femtogram-level sensitivity using absolute quantitation and serial standard curves.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBuilt-in internal control:\u003c\/strong\u003e IPC mix included to monitor inhibition and reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR reaction buffer and primer\/probe mixes minimize setup variability and speed workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus) and adjustable to other qPCR instruments following instrument software guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero (host cell) DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA fragment size distribution during downstream processing and in final product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and release testing in research and development (RUO) workflows\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery studies for host cell DNA assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-85 (NNC020) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-134 (NNC021) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-229 (NNC022) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-552 (NNC023) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProvided documentation\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions total) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read MSDS and user guide before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-decontaminate workspace, use DNase-free consumables, aliquot reagents and change tips between samples.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents as labeled; check expiration date on labels.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific settings and analysis parameters; adjust thresholds\/baselines per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: standards (ST1–ST5), NTC, NCS and IPC for each run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed for femtogram-level sensitivity. The supplied standard curve includes concentrations down to 0.03 pg\/µL (ST5). Exact LOD\/LOQ should be established during laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500 (with ROX), Bio-Rad CFX96 and LineGene 9600plus. Other real-time PCR instruments are supported; follow instrument-specific setup and dye assignments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted and what are acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSet up an absolute quantitation standard curve (ST1–ST5). IPC Ct for samples should be within ±1.0 of the NCS IPC Ct; significant deviation indicates inhibition. NTC Ct should be at least 2 Ct higher than the mean Ct of the lowest standard (or per laboratory acceptance criteria).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw Vero DNA Control and DDB on ice. Prepare ST0 at 3000 pg\/µL using DDB, then perform serial 1:10 dilutions to obtain ST1=300 pg\/µL, ST2=30 pg\/µL, ST3=3 pg\/µL, ST4=0.3 pg\/µL and ST5=0.03 pg\/µL. Include at least five standard points (suggested ST1–ST5) in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189155881141,"sku":"1103174","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Size_Analysis_Kit_2G.jpg?v=1742290678"},{"product_id":"residual-hek293-dna-size-analysis-kit-shentek™","title":"Residual HEK293 DNA Size Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HEK293 DNA Size Analysis Kit is designed to quantify residual HEK293 host cell DNA fragments of different sizes across biopharmaceutical sample types (in-process samples to final products) using real-time PCR (qPCR). The kit amplifies four fragment sizes (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer\/probe mixes for each fragment, internal positive control (IPC), DNA dilution buffer and a HEK293 DNA control for standard curve preparation. For extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eReal-time PCR (qPCR) with TaqMan-style primer \u0026amp; probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned for quantitation at the femtogram (fg) level (lowest standard in kit workflow corresponds to 0.03 pg\/µL = 30 fg)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (prepared from supplied HEK293 DNA Control)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock prior to dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear \/ working range (as provided by recommended standards)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard points from 0.03 pg\/µL to 300 pg\/µL (prepare standard curve using ST1–ST5); ST0 (3000 pg\/µL) used as stock\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR master mix + 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; HEK293 Primer\u0026amp;Probe MIX (appropriate fragment) 2.8 μL; IPC MIX 1.3 μL; total 20 μL (then add 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/read: 72 °C 01:30; 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, FQD-96A and similar real-time PCR instruments (user must configure detectors\/channels accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC \/ quality control criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. Ct of NTC and NCS should be larger than the mean Ct of the lowest concentration standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability \/ shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Simultaneous assays for four discrete fragment lengths (75, 122, 244, 562 bp) allow determination of residual HEK293 DNA size distribution.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify DNA down to the femtogram range (lowest recommended standard 0.03 pg\/µL = 30 fg).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated HEK293 Primer\u0026amp;Probe MIXes for each fragment size reduce setup time and minimize variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control (IPC):\u003c\/strong\u003e IPC MIX included to monitor inhibition and assay performance; acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible instrument compatibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (instructions include detector\/channel setup and reference dye settings).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA dilution buffer and HEK293 DNA control for standard curve preparation; refers to complementary sample prep kit for extraction.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical process and final product testing\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and process development for biologics produced in HEK293 cells\u003c\/li\u003e\r\n \u003cli\u003eRelease testing and quality control (research-use contexts)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-75 (NNC012), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-122 (NNC013), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-244 (NNC014), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-562 (NNC015), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after thaw; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control (NNA005), 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment-specific primer\/probe mix (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points (recommended ST1–ST5) and run samples in triplicate where possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR run setup: reaction volume 30 μL (20 μL master mix + 10 μL template); thermal cycling and detector settings must be configured per instrument.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: sample Ct-IPC should be within ±1.0 of NCS Ct-IPC — higher Ct-IPC indicates potential inhibition; consider recovery controls.\u003c\/p\u003e\r\n \u003cp\u003e• Negative controls (NTC) and NCS should show Ct values greater (later) than the mean Ct of the lowest standard point.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity \/ limit of detection (LOD) of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to quantify HEK293 DNA at the femtogram level. The supplied standard curve includes points down to 0.03 pg\/µL (30 fg); actual LOD\/LOQ should be established during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which fragment sizes are measured and how are they assayed?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFour fragment sizes are assayed separately using fragment-specific primer \u0026amp; probe mixes: 75 bp, 122 bp, 244 bp and 562 bp. Prepare four separate standard curves (one per fragment) and run each assay on the qPCR instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems such as SHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, and FQD-96A. Users must configure detectors\/channels, passive reference dye (e.g., ROX if required), and cycling conditions according to their instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibited?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (internal positive control). The sample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. A significantly higher Ct-IPC in the sample suggests inhibition; dilute the sample or perform recovery controls and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189170954421,"sku":"1103176","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Size_Analysis_Kit.jpg?v=1742291951"},{"product_id":"residual-host-cell-rna-sample-preparation-kit-shentek™","title":"Residual Host Cell RNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature; 2-8 °C; -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell RNA Sample Preparation Kit is a magnetic particle-based RNA extraction kit for isolation of residual host cell RNA from biological products (e.g., E. coli, 293T). The kit is compatible with manual workflows and automated extraction on the rHCDpurify system. Extracted RNA is suitable for downstream RNA quantitation (real-time qPCR) and other nucleic acid-based assays. The kit includes reagents for protein digestion (Proteinase K), binding, washing, magnetic particles, RNase inhibitor, glycogen carrier, and elution reagents. The kit is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eExtraction of residual host cell RNA from biological samples for downstream RNA quantitation (qPCR)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMagnetic particle-based RNA binding, wash and elution\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003eTypical: add 100 µL sample + 110 µL Proteinase K digestion solution; incubate 55 °C for 60 min; machine max digestion volume 300 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K dosing guidance\u003c\/td\u003e\r\n \u003ctd\u003eFor sample protein concentration 0–100 mg\/mL: 10 µL Proteinase K per sample; for 100–200 mg\/mL: 20 µL Proteinase K per sample (volumes mixed with Proteinase K Buffer as tabled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding\u003c\/td\u003e\r\n \u003ctd\u003ePer sample: add 10 µL 5M NaCl + 220 µL working binding solution; add 200–400 µL isopropanol (≈1\/3–1\/2 total volume); add 10–15 µL magnetic particles; mix 3–5 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWashing\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL; air-dry magnetic particles 30 s–3 min to remove residual ethanol\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAdd 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v); incubate 50 °C for 5 min with intermittent vortexing; separate beads and recover eluate\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003eUse 10–15 µL per sample; vortex to re-suspend before use; recommended storage 2–8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking binding prep\u003c\/td\u003e\r\n \u003ctd\u003ePer sample working binding: 200 µL Binding solution + 10 µL diluted Precipitation solution I (1:99 in Dilution solution) + 10 µL Glycogen\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH requirement\u003c\/td\u003e\r\n \u003ctd\u003eIdeal sample pH 6.0–8.0; adjust with 1 M HCl or 1 M NaOH if pH \u0026lt;5.0 or \u0026gt;9.0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures are stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility \/ equipment\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with rHCDpurify automated system and manual extraction; requires real-time PCR system for downstream quantitation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle-based separation:\u003c\/strong\u003e Efficient magnetic bead capture of RNA for high recovery and clean eluates suitable for qPCR\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAutomated and manual compatibility:\u003c\/strong\u003e Validated for use with rHCDpurify instrument and a manual workflow\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for RNA extraction:\u003c\/strong\u003e Includes Proteinase K digestion reagents, binding\/wash\/elution buffers, magnetic particles, RNase inhibitor and glycogen carrier\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for residual host cell RNA:\u003c\/strong\u003e Optimized procedure for extraction of residual RNA from biological products and plasmid preparations\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStable shelf life:\u003c\/strong\u003e Components stored at proper temperatures are stable up to 24 months\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell RNA from biopharmaceutical samples (e.g., E. coli, 293T)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for RNA quantitation by real-time qPCR\u003c\/li\u003e\r\n \u003cli\u003ePreparation of plasmid DNA samples with residual host RNA assessment\u003c\/li\u003e\r\n \u003cli\u003eRoutine QC workflows in research and development laboratories\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016) 20 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014) 30 mL × 1 (requires addition of 40 mL ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution solution (NND061) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution solution (NND062) 12 mL × 2\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase inhibitor (NND060) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePrecipitation solution I (NND003) 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based RNA extraction (manual or rHCDpurify automated)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: gloves, protective eyewear, mask and lab clothing.\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL ethanol to Wash buffer A before first use; prepare 70% ethanol Wash buffer B from Dilution solution.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare working elution solution by mixing RNase inhibitor : Elution solution = 1 : 200 (v:v) in RNase-free area.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare diluted Precipitation solution I at 1:99 (Precipitation solution I : Dilution solution) before preparing working binding solution.\u003c\/p\u003e\r\n \u003cp\u003e• Ideal sample pH is 6.0–8.0. Adjust samples to neutral pH if outside range.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry magnetic particles; over-drying may reduce elution efficiency.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day as extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• When removing supernatant, do not disturb or aspirate magnetic bead pellet; use low-retention tips and centrifuge as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• UV sterilize rHCDpurify instrument interior (suggested 15 minutes) before runs; allow \u0026gt;30 min between extractions if required.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201205.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201205.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What downstream assays are compatible with the extracted RNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted RNA is suitable for RNA quantitation by real-time qPCR and other nucleic-acid based assays. Use appropriate DNase treatment if required by your assay.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare Wash buffer A and B before first use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 40 mL of ethanol to Wash buffer A (NND014) before first use. Prepare Wash buffer B by mixing Dilution solution (NND062) with ethanol to make 70% ethanol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended elution volumes and conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v). Incubate at 50 °C for 5 minutes with intermittent vortexing, then separate beads and recover eluate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this kit be used on an automated platform?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify automated extraction system (program rHCR-1201205) as well as manual extraction workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45189191270581,"sku":"1201205","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_RNA_Sample_Preparation_Kit.jpg?v=1742293165"},{"product_id":"residual-e-coli-rna-quantitation-kit-2g-shentek™","title":"Residual E.coli RNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli RNA Quantitation Kit (2G) is designed for quantitation of host cell RNA from Escherichia coli in biopharmaceutical products. The kit employs a duplex reverse transcription quantitative PCR (qRT-PCR) format including a target detector (FAM) and an Internal Positive Control (IPC, VIC) in the E. coli RNA Primer \u0026amp; Probe MIX. The assay provides rapid, specific and reliable quantitation with femtogram-level sensitivity for the target gene. The kit includes One Step qPCR Buffer, One Step Enzyme MIX, Primer \u0026amp; Probe MIX (incl. IPC), E. coli RNA Control and RNase-free water, and is compatible with common real-time PCR instruments.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry\u003c\/td\u003e\r\n \u003ctd\u003eDuplex one-step reverse transcription quantitative PCR (one-step RT-qPCR) with FAM-labeled target and VIC-labeled IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ Limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget detectable at femtogram (fg) level (document states target gene can be determined at the femtogram level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared from 2000 pg\/µL (stock) producing working standards: 20, 2, 0.2, 0.02, 0.002 pg\/µL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 20 µL (15 µL qRT-PCR MIX + 5 µL sample\/standard\/controls)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR mix composition per well\u003c\/td\u003e\r\n \u003ctd\u003eOne Step qPCR Buffer 10 µL, One Step Enzyme MIX 1 µL, E. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) 4 µL — total 15 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 0:30 (1 cycle); 45 cycles of 95 °C 0:10 (denature) and 60 °C 0:40 (anneal\/extend, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold 0.05; Automatic baseline; ROX as passive reference (for ABI 7500), set Standard Curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be at least 2 cycles higher than the lowest standard; ERC recovery acceptance 50%–150%; compare sample IPC Ct to NTC\/NCS IPC Ct to evaluate inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase digestion conditions (for plasmid samples)\u003c\/td\u003e\r\n \u003ctd\u003eIncubate samples with DNase I at 37 °C for 30–60 minutes; recommended DNase I final concentration 0.2–2 U\/µL depending on sample; ensure plasmid sample ≤100 ng\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase inactivation\u003c\/td\u003e\r\n \u003ctd\u003eOptions: (1) SHENTEK® Residual Host Cell RNA Sample Preparation Kit purification; (2) heat inactivation at 75 °C for 10 minutes; (3) other validated methods\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability (kit)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at –20 °C; kit components stable up to 24 months (check label expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex one‑step RT-qPCR:\u003c\/strong\u003e Combined reverse transcription and qPCR in a single reaction for speed and reduced handling\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Target detection at femtogram (fg) level enabling quantitation of trace residual E. coli RNA\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included in Primer \u0026amp; Probe MIX (VIC) to monitor PCR performance and potential inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated buffer, enzyme mix and primer\/probe mix to simplify setup and improve reproducibility\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflow compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, Bio-Rad CFX96)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow for biopharmaceutical samples:\u003c\/strong\u003e Protocols and optional sample prep kit provided for plasmid DNA and protein expression products\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual host cell RNA (HCR) from Escherichia coli in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in plasmid DNA preparations\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in protein expression products derived from E. coli\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and in-process or release testing for downstream product quality control\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Control (NNA011) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) (NNC119) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 100 Reactions | Method: qRT-PCR (duplex one-step reverse transcription quantitative PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive components (buffer and enzyme mix) from light; store all kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice and mix gently; avoid repeated freeze-thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Work in RNase-free conditions: use RNase\/DNase-free consumables, decontaminate surfaces and irradiate equipment with UV for 30 minutes, disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Perform DNase I treatment to remove genomic DNA for plasmid-derived samples; incubate at 37 °C for 30–60 minutes and inactivate DNase I (e.g., 75 °C for 10 minutes or purification).\u003c\/p\u003e\r\n \u003cp\u003e• Ensure final plasmid concentration ≤100 ng\/µL when performing DNase digestion; use DNase I final concentration 0.2–2 U\/µL as appropriate.\u003c\/p\u003e\r\n \u003cp\u003e• Include controls: standards (ST1–ST5), NTC, NCS and ERC; ERC recovery should be within 50%–150% to accept sample results.\u003c\/p\u003e\r\n \u003cp\u003e• If sample IPC Ct is significantly higher than NTC\/NCS IPC Ct, sample inhibition is possible; interpret IPC as reference and consider sample recovery.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect the target gene at the femtogram (fg) level. Use the provided standard curve (typical working standards: 20 to 0.002 pg\/µL) to determine assay sensitivity in your system and matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, Applied Biosystems 7500, Roche LightCycler 480 II and Bio-Rad CFX96. Program settings may require adjustment per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples derived from plasmid DNA be prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePlasmid-derived samples require DNase I digestion to remove genomic DNA (37 °C for 30–60 min). After digestion, inactivate or remove DNase I (e.g., heat 75 °C for 10 min or use the SHENTEK Residual Host Cell RNA Sample Preparation Kit) before qRT-PCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a standard curve (ST1–ST5), no template control (NTC), negative control sample (NCS), and extraction recovery control (ERC). NTC Ct should be ≥2 cycles above the lowest standard; ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189194055861,"sku":"1201201-1","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E.coli_RNA_Quantitation_Kit_2G.jpg?v=1742293356"},{"product_id":"mycobacteria-dna-extraction-kit-mycoshentek™","title":"Mycobacteria DNA Extraction Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 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8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eApproximately 50 minutes\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature or 2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycobacteria DNA Extraction Kit is designed for extraction of trace Mycobacteria DNA from a variety of biologic sample types. The kit supports direct extraction from sample volumes ≤400 μL or concentration of larger volumes by centrifugation prior to extraction. It is compatible with manual magnetic-particle based workflows and automated extraction using the rHCDpurify\/rDNApurify instrument. The kit workflow includes pretreatment, Proteinase K digestion, magnetic particle binding (with isopropanol), two wash steps (ethanol-based), and heat elution to produce PCR-ready DNA (minimum recommended eluate ≥40 μL). The kit is supplied for research use only and is intended for downstream molecular detection (e.g., real-time PCR) with MycoSHENTEK detection reagents or other PCR systems.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eProduct No.: 1503601; reagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eExtraction of Mycobacteria DNA from cell-free and cell culture samples for downstream molecular detection (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003eRecommended ≤400 μL per sample; if \u0026gt;400 μL concentrate to ≈400 μL by centrifugation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K\u003c\/td\u003e\r\n \u003ctd\u003eAdd 20 μL Proteinase K per sample during digestion\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLysis\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100 μL Lysis buffer; incubate at 55 °C for 60 min (digestion)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 μL Binding solution + 200 μL isopropanol + 50 μL Magnetic particles per sample; shake 5 min for binding\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWashes\u003c\/td\u003e\r\n \u003ctd\u003eWash 1: 700 μL Wash buffer A (ethanol added before use); Wash 2: 700 μL Wash buffer B (70% ethanol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 50 μL Elution buffer; incubate at 70 °C for 7 min; recover at least 40 μL eluate\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomated run time\u003c\/td\u003e\r\n \u003ctd\u003erHCDpurify \/ rDNApurify program: ~50 minutes (plate-based automated run)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCentrifugation speeds \/ times\u003c\/td\u003e\r\n \u003ctd\u003eCell-free sample concentration: 16000×g for 15 min; Cell pelleting: 72×g for 5 min; Debris precipitation: 450×g for 10–15 min; brief spins (3 s) used frequently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particle separation time\u003c\/td\u003e\r\n \u003ctd\u003eSeparation typically 3–5 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAir-dry time (post-wash)\u003c\/td\u003e\r\n \u003ctd\u003e30 s to 3 min depending on environment; avoid over-drying\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components storable under indicated conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTrace DNA extraction:\u003c\/strong\u003e Optimized to extract low-abundance (trace) Mycobacteria DNA from various biologic matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic bead-based purification:\u003c\/strong\u003e Magnetic particle chemistry provides efficient capture and wash-based purification compatible with manual and automated workflows.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Validated for manual tube-based extraction and automated plate-based extraction using rHCDpurify \/ rDNApurify instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePCR-ready eluate:\u003c\/strong\u003e Produces a concentrated eluate (recommended ≥40 μL) suitable for downstream real-time PCR detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRoom-stable major reagents:\u003c\/strong\u003e Most reagents are stored at room temperature; selected reagents (e.g., cell lysis\/pretreatment\/magnetic particles) refrigerated as indicated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow reagents included:\u003c\/strong\u003e Includes lysis, binding, wash, elution buffers, proteinase K, magnetic particles and pretreatment reagents for sample types.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell-free biologics (e.g., serum, plasma, cell-free supernatants)\u003c\/li\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell culture samples and cell pellets\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for downstream molecular assays including real-time PCR\/qPCR\u003c\/li\u003e\r\n \u003cli\u003eResearch applications and in-process quality control testing of biologics (Research Use Only)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) — add 20 mL anhydrous ethanol before use\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 15 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Cell lysis buffer (NND039)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eFollow label; 500 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePretreatment buffer (NND041)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND034)\u003c\/span\u003e\u003cstrong\u003eFollow label (equilibrate to room temp before use); 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND024)\u003c\/span\u003e\u003cstrong\u003eFollow label; 1 mL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle-based DNA extraction (manual or automated)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and use appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Use separate, dedicated laboratory areas and equipment for reagent preparation, sample preparation, amplification, and post-amplification to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure ambient temperature ≥22 °C before starting experiments when recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Balance and vortex magnetic particles before use; centrifuge tubes briefly (3 s) when instructed to collect liquid from caps\/walls.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Wash buffer A by adding 20 mL anhydrous ethanol before first use; prepare Wash buffer B as 70% ethanol separately.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry the magnetic particle pellet after ethanol wash; over-drying may make resuspension difficult.\u003c\/p\u003e\r\n \u003cp\u003e• Perform mycobacterial DNA detection assays the same day as extraction to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• For automated runs: perform UV sterilization of the instrument for at least 15 min and clean internal walls with 75% alcohol wipes before and after runs; minimum interval between extractions is 30 min.\u003c\/p\u003e\r\n \u003cp\u003e• After automated extraction, immediately transfer eluate to a new tube and ensure ≥40 μL is recovered for downstream assays.\u003c\/p\u003e\r\n \u003cp\u003e• Dispose of used tips and liquid waste according to laboratory biohazard and local regulations.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for cell-free samples (e.g., serum, plasma, supernatants) and cell culture samples (cell pellets\/resuspensions). Samples ≤400 μL can be processed directly; larger volumes should be concentrated to ≈400 μL before extraction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended elution volume and expected yield for downstream PCR?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandard elution uses 50 μL Elution buffer with incubation at 70 °C for 7 minutes. Recover at least 40 μL eluate for downstream PCR\/qPCR applications.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I run the kit on an automated instrument?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with rHCDpurify \/ rDNApurify automated plate-based instruments. The automated program run time is approximately 50 minutes for a typical plate protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should magnetic particles be handled before use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eEquilibrate magnetic particles to room temperature for 10 minutes and vortex thoroughly to fully resuspend particles before aliquoting to samples to ensure consistent binding performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — For research purposes; not for diagnostic use\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45189176230069,"sku":"1503601","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Extraction_Kit.jpg?v=1742292295"},{"product_id":"mycobacteria-dna-detection-kit-mycoshentek™","title":"Mycobacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK® Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, and similar materials using real-time qPCR technology. The integrated system has been validated with a limit of detection of 10–100 CFU\/mL and can detect more than 100 different Mycobacteria species. The assay chemistry includes dUTP and is compatible with UNG (uracil-N-glycosylase) treatment to prevent carry-over contamination. An Internal Control (IC) is provided to monitor PCR inhibition and\/or extraction efficiency. The kit is supplied with positive and negative controls and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd\u003e1503602\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit Format \/ Reactions\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 Reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQualitative detection of Mycobacteria contamination in cells and cell culture-derived biologicals\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated at 10–100 CFU\/mL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n \u003ctd\u003eValidated to detect \u0026gt;100 Mycobacteria species\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay type\u003c\/td\u003e\r\n \u003ctd\u003eReal-time qPCR (probe-based)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL (10 µL qPCR MIX + 20 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eMB qPCR Reaction Buffer 8 µL; MB Primer \u0026amp; Probe MIX 1.5 µL; Internal Control (IC) 0.5 µL; total 10 µL. UNG optional (0.1 U per reaction).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG 25 °C 10:00 (1 cycle); Activation 95 °C 10:00 (1 cycle); 45 cycles of Denaturation 95 °C 00:15, Annealing 60 °C 00:30, Extension 72 °C 01:30 (read fluorescence during extension).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls\u003c\/td\u003e\r\n \u003ctd\u003ePositive Control (PC), No Template Control (NTC), Negative Control Sample (NCS), Internal Control (IC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\r\n \u003ctd\u003eTypical interpretation: FAM positive cutoff Ct \u0026lt;40.00 (positive if at least one well Ct\u0026lt;40 and normal amplification); VIC (IC) expected Ct \u0026lt;35.00 for normal amplification; PC Ct \u0026lt;35.00. NTC FAM Ct ≥40.00 or no amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, and other real-time PCR systems supporting probe detection\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Validated limit of detection down to 10–100 CFU\/mL for broad Mycobacteria detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed and validated to detect over 100 different Mycobacteria species.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarry-over contamination control:\u003c\/strong\u003e Chemistry includes dUTP and is compatible with UNG treatment to prevent and eliminate carry-over contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IC):\u003c\/strong\u003e IC monitors PCR inhibition and\/or extraction efficiency; can be added pre-extraction or to the PCR mix.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-validated workflow:\u003c\/strong\u003e Includes positive and negative controls and recommended plate layouts and acceptance criteria for straightforward result interpretation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQualitative detection of Mycobacteria contamination in cell lines\u003c\/li\u003e\r\n \u003cli\u003eScreening of cell culture-derived biologicals for Mycobacteria contamination\u003c\/li\u003e\r\n \u003cli\u003eRoutine mycoplasma\/contaminant monitoring workflows in research laboratories (Mycobacteria-specific)\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of Mycobacteria DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB qPCR Reaction Buffer (NNB011)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light; 400 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Primer \u0026amp; Probe MIX (NNC035)\u003c\/span\u003e\u003cstrong\u003e2–8 °C until use or -20 °C; 75 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Internal Control (IC) (NNA037)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or -20 °C; 600 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Positive Control (PC) (NNA041)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or room temperature per label; 1.5 mL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use UV decontamination (30 minutes) and 75% ethanol to clean surfaces and equipment before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use UNG enzyme optionally to reduce carry-over contamination (check effective amount before use).\u003c\/p\u003e\r\n \u003cp\u003e• If VIC (IC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout and include PC, NTC and NCS as described; use duplicates for samples and controls.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe integrated system has been validated with a limit of detection of approximately 10–100 CFU\/mL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the kit detect multiple Mycobacteria species?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The assay has been validated to detect more than 100 different Mycobacteria species.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is an Internal Control provided and how should it be used?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAn Internal Control (IC) is provided. It can be added during sample extraction to monitor extraction efficiency or added to the PCR mix to monitor PCR inhibition. If IC was added during extraction, replace IC volume with DNA dilution buffer when preparing the qPCR mix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended instruments and reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500). Prepare 30 µL reaction volumes (10 µL qPCR MIX + 20 µL sample\/control) and run the recommended cycling program with UNG treatment if used.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"Default Title","offer_id":43795617874101,"sku":"1503602","price":1525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Detection_Kit.jpg?v=1742292467"},{"product_id":"mycoplasma-dna-extraction-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature \/ 2–8 °C \/ -20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) is a magnetic particle–based DNA extraction kit designed for isolation of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks and complex sample matrices (e.g., up to 10^7 cells culture, 5% human albumin solutions, high-concentration plasmids). The kit is supplied for 50 extractions and is intended to be used in an integrated workflow together with the MycoSHENTEK® Mycoplasma Detection Kit (2G). The method is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection. The kit supports manual extraction and automated extraction using the rHCDpurify system. The validated detection limit is 10 CFU\/mL. The protocol includes sample pretreatment, enzymatic digestion (Proteinase K), magnetic-particle binding, wash steps, and elution to produce purified DNA suitable for downstream real-time PCR assays.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended use \/ scope\u003c\/td\u003e\n \u003ctd\u003eExtraction of trace mycoplasma DNA from cell banks, virus seed banks and complex matrices (cell cultures, 5% human albumin, high-concentration plasmids).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidated standards\u003c\/td\u003e\n \u003ctd\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated when used with MycoSHENTEK® Mycoplasma Detection Kit (2G)).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample input volume\u003c\/td\u003e\n \u003ctd\u003eUp to ~400 µL directly; larger volumes should be concentrated by centrifugation and reduced to ~400 µL prior to extraction.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 50 extractions.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTypical digestion\/incubation conditions\u003c\/td\u003e\n \u003ctd\u003eAdd 20 µL Proteinase K and 100 µL Lysis buffer; incubate 55°C for 60 min for most matrices; for 5% human albumin incubate at 25°C for 60 min.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eBinding step\u003c\/td\u003e\n \u003ctd\u003ePer sample add 200 µL Binding Buffer, 200 µL isopropanol and 30 µL Magnetic particles; vertical mixing ~5 min to bind nucleic acids.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eWash steps\u003c\/td\u003e\n \u003ctd\u003eTwo washes: 700 µL Wash buffer A then 700 µL Wash buffer B; remove residual ethanol and air-dry magnetic pellet briefly (30 s–3 min).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eElution\u003c\/td\u003e\n \u003ctd\u003eElute with 50 µL Elution buffer; incubate at 70°C for 7 min with intermittent vortexing; recover eluate (~40 µL+ recommended for downstream assay).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAutomated run time (rHCDpurify)\u003c\/td\u003e\n \u003ctd\u003eApprox. 43 minutes per plate run (program: Myco-2G).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage stability\u003c\/td\u003e\n \u003ctd\u003eKit components stable up to 24 months when stored under recommended conditions (see component list).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCritical centrifugation speeds\u003c\/td\u003e\n \u003ctd\u003eDebris removal: 7,250×g (10 s); concentration and plasmid\/cell concentration steps: 18,000×g (30 min); microcentrifuge brief spins: 3–10 s for liquid collection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls\u003c\/td\u003e\n \u003ctd\u003eNegative control sample (NCS) procedure provided; Internal Control (IC) (2G) is used during extraction (IC is supplied in the companion detection kit).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated detection limit of 10 CFU\/mL when used with MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad matrix compatibility:\u003c\/strong\u003e Works with cell cultures (up to 10^7 cells), cell culture supernatants, 5% human albumin solutions, high-concentration plasmids and other complex matrices.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports both manual magnetic-particle extraction and automated extraction on the rHCDpurify system.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeial validation:\u003c\/strong\u003e Validated in accordance with USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection workflows.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eComplete extraction chemistry:\u003c\/strong\u003e Includes lysis, binding, wash and elution reagents plus magnetic particles and proteinase K for robust nucleic acid recovery from low-copy targets.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eMycoplasma testing of master cell banks (MCBs) and working cell banks (WCBs)\u003c\/li\u003e\n \u003cli\u003eMycoplasma testing of virus seed banks\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture samples (up to 10^7 cells)\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture supernatants\u003c\/li\u003e\n \u003cli\u003eTesting of samples containing 5% human albumin\u003c\/li\u003e\n \u003cli\u003eExtraction from high-concentration plasmid preparations prior to mycoplasma detection\u003c\/li\u003e\n \u003cli\u003eSample preparation prior to real-time PCR-based mycoplasma detection\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (after adding 20 mL anhydrous ethanol prior to first use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (heat at 37°C if cloudy)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePretreatment buffer (NND002) — 1.25 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMagnetic particles (NND033) — 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003e5 M NaCl (NND040) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution I (NND003) — 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature (dilute 1:99 in DNA Dilution Buffer prior to use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution II (NND004) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eProteinase K (NND023) — 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C \/ according to label (keep frozen until use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based DNA extraction (magnetic bead chemistry) for downstream qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• Read MSDS and use appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\n \u003cp\u003e• Divide lab into separate areas (negative\/preparation, positive\/sample prep, amplification) and use dedicated equipment to prevent cross-contamination.\u003c\/p\u003e\n \u003cp\u003e• Ensure ambient temperature is not lower than 22°C before starting experiments (per manual recommendation).\u003c\/p\u003e\n \u003cp\u003e• Centrifuge tubes before opening to avoid splashing; handle tubes carefully to prevent contamination.\u003c\/p\u003e\n \u003cp\u003e• Change gloves frequently and use different gloves\/lab coats in separate areas.\u003c\/p\u003e\n \u003cp\u003e• Dispose of used tips and liquid waste according to local regulations; do not open post-PCR products in amplification area.\u003c\/p\u003e\n \u003cp\u003e• Avoid over-drying magnetic particle pellet during ethanol removal to prevent incomplete elution.\u003c\/p\u003e\n \u003cp\u003e• Perform DNA detection the same day as extraction to ensure accuracy.\u003c\/p\u003e\n \u003cp\u003e• For automated runs on rHCDpurify: UV sterilize the instrument for ≥15 minutes and clean inner walls with 75% ethanol wipes before and after runs; minimum interval between extractions should be 30 minutes.\u003c\/p\u003e\n \u003cp\u003e• When removing supernatants, avoid aspirating magnetic particles; use magnetic stand and allow full particle separation (3–5 min) before removing supernatant.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection when this extraction kit is used with the MycoSHENTEK® Mycoplasma Detection Kit (2G)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe workflow is validated with a detection limit of 10 CFU\/mL when used with the MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I process sample volumes larger than 400 µL?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. For sample volumes \u0026gt;400 µL the protocol requires concentration by centrifugation to a final volume of approximately 400 µL before extraction.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which samples require a different digestion temperature?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMost sample types are incubated with Proteinase K at 55°C for 60 minutes. Samples containing 5% human albumin and their controls should be incubated at 25°C for 60 minutes.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the kit compatible with automation?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with automated extraction using the rHCDpurify system (example program Myco-2G, ~43 minutes runtime).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45191334133941,"sku":"1509840","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Extraction_Kit_2G.jpg?v=1742362510"},{"product_id":"mycoplasma-dna-detection-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n 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center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20°C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR-based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culture–derived products. The kit is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII, with a stated limit of detection of 10 CFU\/mL. The assay detects \u0026gt;200 species with high specificity and includes Internal Control (IC, 2G) to monitor PCR inhibition and optionally to evaluate extraction efficiency when added prior to extraction. The kit is supplied for 50 reactions and is intended for research use only (RUO).\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eAssay type\u003c\/td\u003e\n \u003ctd\u003eQualitative real-time PCR (qPCR) assay\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\n \u003ctd\u003eDetects \u0026gt;200 species of Mycoplasma, Spiroplasma and Acholeplasma\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls included\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eInternal Control (IC) (2G), Positive Control (PC) (2G)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction format \u0026amp; volume\u003c\/td\u003e\n \u003ctd\u003e30 μL total reaction volume: 10 μL qPCR MIX + 20 μL sample (per well)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR Mix composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eMy qPCR Reaction Buffer (2G) 8 μL; My Primer \u0026amp; Probe MIX (2G) 1.5 μL; Internal Control (IC) (2G) 0.5 μL (total 10 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n \u003ctd\u003eUNG 25°C 10:00 (1); Activation 95°C 10:00 (1); 45 cycles of: Denaturation 95°C 00:15, Annealing 62°C 00:30, Extension 72°C 01:30 (fluorescence read during extension)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection channels \/ dyes\u003c\/td\u003e\n \u003ctd\u003eTarget: FAM; Internal Control: VIC; ROX optional as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, Roche LightCycler 480 and other real-time PCR systems\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended sample types \/ matrices\u003c\/td\u003e\n \u003ctd\u003eMaster cell bank, working cell bank, virus seed stock, cell culture-derived products and similar matrices (validated across a variety of matrices)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n \u003ctd\u003e-20°C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReconstitution instructions (controls)\u003c\/td\u003e\n \u003ctd\u003eReconstitute lyophilized IC (2G) in 600 μL DNA Dilution Buffer; reconstitute lyophilized PC (2G) in 500 μL DNA Dilution Buffer\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\n \u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35; PC: FAM Ct \u0026lt; 35; Unknown positive: FAM Ct \u0026lt; 40 (≥1 well) with normal amplification; If VIC inhibited, repeat extraction or remove inhibitors\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated LOD of 10 CFU\/mL allowing detection of low-level mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed to detect more than 200 Mycoplasma, Spiroplasma and Acholeplasma species with high specificity\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition and extraction monitoring:\u003c\/strong\u003e Internal Control (IC, 2G) can be added to PCR or to the sample prior to extraction to monitor PCR inhibition and evaluate extraction efficiency\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeia standards:\u003c\/strong\u003e Validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Provided My Primer \u0026amp; Probe MIX (2G) simplifies assay setup; lyophilized positive and internal controls included\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (SHENTEK-96S, CFX96, 7500, LightCycler 480, etc.)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQualitative mycoplasma testing of master cell banks and working cell banks\u003c\/li\u003e\n \u003cli\u003eScreening of virus seed stocks for mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture–derived products and biologics for mycoplasma\u003c\/li\u003e\n \u003cli\u003eRoutine cell culture contamination monitoring and QC in R\u0026amp;D and production (research use only)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eInternal Control (IC) (2G) (Part No. NNA035) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 600 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePositive Control (PC) (2G) (Part No. NNA039) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 500 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy qPCR Reaction Buffer (2G) (Part No. NNB004) 400 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy Primer \u0026amp; Probe MIX (2G) (Part No. NNC065) 75 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eUser Guide \/ Instructions for use\u003c\/span\u003e\u003cstrong\u003eRoom temperature (follow handling instructions)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For research use only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean area: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\n \u003cp\u003e• Thaw kit components at 2–8°C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n \u003cp\u003e• Protect My qPCR Reaction Buffer from light during storage and use.\u003c\/p\u003e\n \u003cp\u003e• If Internal Control (IC) was added during extraction, substitute equal volume of DNA Dilution Buffer in qPCR mix instead of IC.\u003c\/p\u003e\n \u003cp\u003e• Follow recommended plate layout and include controls (PC, NTC, NCS) in duplicate.\u003c\/p\u003e\n \u003cp\u003e• If VIC (IC) signal is inhibited (Ct ≥ threshold), repeat extraction or remove inhibitors and repeat assay.\u003c\/p\u003e\n \u003cp\u003e• Dispose of biological and chemical waste according to local regulations.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated with a limit of detection of 10 CFU\/mL.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the Internal Control (IC) be added before extraction?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. IC (2G) may be added to the sample prior to extraction to evaluate extraction efficiency; if IC is added during extraction, omit IC when preparing the qPCR mix and add equal volume of DNA Dilution Buffer instead.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which qPCR instruments are compatible with this kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500 and Roche LightCycler 480. Use instrument software to set FAM for target and VIC for IC.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted (Ct thresholds)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eAcceptance criteria: Positive control (PC) FAM Ct \u0026lt; 35; NTC FAM Ct ≥ 40 or no amplification; Unknown sample: FAM Ct \u0026lt; 40 (at least one well) with normal amplification = Positive. If IC (VIC) is inhibited (VIC Ct ≥ threshold or absent), repeat extraction or remove inhibitors and retest.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45191337345205,"sku":"1509841","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Detection_Kit_2G.jpg?v=1742362689"},{"product_id":"residual-vero-dna-quantitation-kit-shentek™","title":"Residual Vero DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Vero DNA Quantitation Kit is designed for quantitative measurement of residual Vero host cell DNA in biopharmaceutical samples across processing stages, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) with a Vero-specific primer \u0026amp; probe mix to deliver rapid, specific and reliable quantitation down to the femtogram (fg) level. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit provides reagents sufficient for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (Standard curve \/ Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at the femtogram (fg) level (document states femtogram-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST0 (3000 pg\/µL prepared stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL; ST6 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL; Sample\/Standard\/NTC\/NCS addition: 10 µL; Total reaction volume: 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + Vero Primer\u0026amp;Probe MIX 3 µL = 20 µL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector \/ Dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: (none specified)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected; Threshold for Vero-DNA set to 0.02; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ controls\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard; ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycles\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments (examples)\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 (other real-time PCR systems likely compatible after instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed for quantitation down to the femtogram (fg) level for detection of trace residual Vero DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Includes qPCR Reaction Buffer and Vero-specific Primer \u0026amp; Probe MIX to simplify setup and standardization.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePredefined standard curve:\u003c\/strong\u003e Provided Vero DNA Control and recommended serial dilutions (ST1–ST6) for absolute quantitation and standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example instrument settings provided for common real-time PCR systems; parameters can be adjusted per instrument\/software.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControl strategy and QC guidance:\u003c\/strong\u003e Includes instructions for NTC, NCS, and Extraction Reference Control (ERC) with recovery acceptance criteria (50%–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing during biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in Vero cell lines\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 µL × 2 tubes); protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX (NNC016)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and maintain a DNA-free workspace (UV decontamination and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light during storage and handling.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run at least five concentrations on the standard curve; include NTC, NCS and ERC controls as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; ERC recovery should be between 50% and 150%; NCS Ct must be greater than mean Ct of lowest standard or less than validated LOQ if LOQ \u0026lt; lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Configure instrument-specific analysis parameters according to your instrument\/software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the kit sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended to quantify residual Vero DNA down to the femtogram (fg) level. The validated limit of quantitation (LOQ) should be determined by laboratory validation; if LOQ is below the lowest standard, ensure NCS Ct is less than the LOQ concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents sufficient for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended storage conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C. The components are stable for up to 24 months; check individual tube labels for expiration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which qPCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems. The user guide provides example settings for SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and LineGene 9600. For other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187363700917,"sku":"SK030204V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Quantitation_Kit.png?v=1742278593"},{"product_id":"residual-sv40-lta-e1a-dna-quantitation-kit-2g","title":"Residual SV40 LTA\/E1A DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual SV40LTA \u0026amp; E1A DNA Quantitation Kit (2G) is a duplex real-time PCR (qPCR) kit designed to quantify residual SV40 large T antigen (LTA) and E1A host cell DNA (e.g., from HEK293T). The assay is duplex (simultaneous detection of SV40LTA and E1A) with an internal positive control (IPC) and achieves sensitivity to the level of 10^1 copies\/μL. The kit includes linear and non-linear DNA controls for standard curve generation and is compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96). Reagents are provided for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual SV40LTA and E1A host cell DNA in biological samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real-time PCR (qPCR) with target-specific primer\/probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10^1 copies\/μL (stated detection level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 4.67 × 10^6 to 4.67 × 10^1 copies\/μL (ST1–ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (non-linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 2.80 × 10^6 to 2.80 × 10^2 copies\/μL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl concentrations (as supplied)\u003c\/td\u003e\r\n \u003ctd\u003eLinear DNA Control: SV40LTA 4.67 × 10^9 copies\/μL; E1A 4.97 × 10^9 copies\/μL. Non-linear DNA Control: SV40LTA 2.80 × 10^9 copies\/μL; E1A 2.98 × 10^9 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation using standard curve (user-prepared serial dilutions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup \/ volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL sample or standard). qPCR MIX per reaction: qPCR Reaction Buffer 15.9 μL, Primer \u0026amp; Probe MIX 2.8 μL, IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15; Annealing\/extension 60 °C 1:00; 40 cycles. Fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other real-time PCR instruments supported with appropriate settings)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set Standard Curve (Absolute Quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within ±1.0 of the NCS Ct(IPC); significant increase indicates potential inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance thresholds\u003c\/td\u003e\r\n \u003ctd\u003eSV40LTA detection value of NTC should be ≤ 14.32 copies\/μL; E1A detection value of NTC should be ≤ 17.79 copies\/μL (or use user-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR:\u003c\/strong\u003e Simultaneous quantitation of SV40LTA and E1A targets in the same reaction using target-specific probes and an IPC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects down to 10^1 copies\/μL enabling detection of low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use controls and mixes:\u003c\/strong\u003e Includes linear and non-linear DNA controls, qPCR reaction buffer, primer\/probe mix, and IPC mix to support standard curve generation and assay QC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Validated on common qPCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96) and adaptable to other real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-defined analysis settings:\u003c\/strong\u003e Recommended thermal cycling and analysis parameters (e.g., threshold = 0.02, manual Ct) provided to standardize quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual SV40LTA and E1A host cell DNA in cell substrates (e.g., HEK293T)\u003c\/li\u003e\r\n \u003cli\u003eResidual host cell DNA monitoring for upstream\/downstream bioprocessing and product release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and standard curve generation for absolute quantitation of SV40-derived sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A linear DNA Control (NNA019) — lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A non-linear DNA Control (NNA020) — 50 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A Primer \u0026amp; Probe MIX (NNC030) — 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) — 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after opening per protocol)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect surfaces with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Reconstitute lyophilized control carefully: add 55 μL ddH2O, mix and let stand 10 minutes; perform serial dilutions as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer \u0026amp; Probe MIX, IPC MIX) from light and store at –20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five concentration points for the standard curve; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• DDB: store remaining unused DDB at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant shifts indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: SV40LTA ≤ 14.32 copies\/μL and E1A ≤ 17.79 copies\/μL (or use user-defined validation criteria).\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters per instrument and software version; recommended settings include manual Ct and threshold = 0.02.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit reports sensitivity to the level of 10^1 copies\/μL. Actual LOD\/LOQ should be confirmed during your laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500 (SDS v1.4 example) and Bio-Rad CFX96. Other instruments may be used with appropriate program and analysis adjustments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the qPCR thermal profile and analysis?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended program: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 (read fluorescence during annealing\/extension). Analysis: Manual Ct with threshold = 0.02 and Automatic Baseline; use Absolute Quantitation (standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I include and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude NTC, NCS, and at least five standard curve points (ST1–ST5; ST6 optional for linear control). IPC mean Ct should be within ±1.0 of NCS. NTC detection values should be ≤ 14.32 copies\/μL for SV40LTA and ≤ 17.79 copies\/μL for E1A, or use your validated criteria.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45191440335029,"sku":"1403443","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_SV40_LTA_E1A_DNA_Quantitation_Kit_2G.webp?v=1742280022"},{"product_id":"advshentek-detectinnova-system","title":"AdvSHENTEK DetectInnova System SHENTEK™","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eRapid, Simple, Accurate Mycoplasma Detection to Anyone \u0026amp; Anywhere\u003c\/p\u003e\n\u003cp\u003eThe AdvSHENTEK DetectInnova System transformed RMM  (rapid microbiological method) into a streamlined, fast, fully automated, and easily portable laboratory system. It integrates nucleic acid extraction and real-time PCR assay, allowing a turn-around time of 2.5 hours from sample to result. Importantly, it only requires less than 5 minutes of hands-on time without specific DNA extraction and PCR expertise to operate.\u003c\/p\u003e\n\u003cp\u003eFor Mycoplasma cassette information, please refer to the SHENTEK® AdvSHENTEK Mycoplasma DetectInnova Cassette User Guide (Product No. 1509100).\u003c\/p\u003e\n\u003cp\u003eSize: 4 system; 16 system\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/advshentek-mycoplasma-detectInnova-system_600x600.png?v=1741751390\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eEasy on-time testing:\u003cbr\u003e\u003c\/strong\u003eComplete automation from sample to result;\u003cbr\u003eHands-on time \u0026lt; 5 mins;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Sensitivity \u0026amp; Efficiency:\u003cbr\u003e\u003c\/strong\u003eLOD \u0026lt; 10 CFU\/mL;\u003cbr\u003e4 standardized assays in a single run;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAccessible Anywhere \u0026amp;Anyone:\u003cbr\u003e\u003c\/strong\u003eNo PCR lab or skills required;\u003cbr\u003eSeamless integration from extraction to amplification without manual intervention;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eData Reliability:\u003cbr\u003e\u003c\/strong\u003eFully validated workflow;\u003cbr\u003eEffective internal control across every stage;\u003cbr\u003e21 CFR Part 11 compliance;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHigh Specificity:\u003cbr\u003e\u003c\/strong\u003eNo cross-reactivity with different bacteria or cell lines;\u003cbr\u003eNo sample matrix interference;\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eComprehensive coverage:\u003cbr\u003e\u003c\/strong\u003eAble to detect over 180 species of Mycoplasma, Spiroplasma and Acholeplasma;\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/advshentek-detectinnova-system_08b52161-2a60-4af1-852e-96b01f4ce431_600x600.jpg?v=1741751586\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 style=\"text-align: start;\"\u003eSpecificity- AdvSHENTEK DetectInnova System\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e2.1 Strain Coverage:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThrough intensive bioinformatics analysis of the amplification targets, the detection system allows genomic DNA of nearly 200 species, including 135 species of Mycoplasma, 38 species of Spiroplasma, and 8 species of Acholeplasma.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.2 Strain Specificity:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eNo cross-reactivity with Clostridium, Lactobacillus, Streptococcus, and different cell lines.\u003cbr\u003eTable 3 Results of Cross-Reactivity\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCross\u003cbr\u003ereactant NO.\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eResource\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCross\u003cbr\u003eReactivity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCHO\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eVero\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e293T\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHEK293\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e5\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eMDCK\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e6\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNS0\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e7\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSf9\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e8\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHi5\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e9\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eE.coli\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003ePichia pastoris\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e11\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSaccharomyces cerevisiae\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e12\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eHans enula polymorpha\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e13\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eClostridium acetobutylicum\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e14\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eClostridium perfringens\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e15\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eClostridium sporogenes\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e16\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStreptococcus pneumoniae\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e17\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStreptococcus agalactiae\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e18\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStreptococcus mutans\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2.3 Matrix Specificity: No Matrix Effect in Complex\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTable 4 Results of Sample Matrix Interference\u003c\/p\u003e\n\u003cp\u003eList of culture media, serum, and cryoprotectants tested do not interfere with mycoplasma detection using the SHENTEK DetectInnova system.\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 64px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 3px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNO\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSample Matrix\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSignal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 3px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eExpiCHOTM\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 3px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eExpiCHOTM(10%DMSO)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 3px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e3\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDMEM(10%FBS)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 3px;\"\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003e4\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDMEM(20%FBS+10%DMSO)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; height: 3px; text-align: center; vertical-align: middle;\"\u003eN\/A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Robustness: Detection Limit Less Than 10 CFU\/mLin Complex Matrices\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eTable 5 Matrix robustness results\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eStrain\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eMatrix\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eTset Conc.\u003cbr\u003e(CFU\/mL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eDetection rate\u003cbr\u003e(%)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eAcceptance\u003cbr\u003eCriteria\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eConclusion\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eMycoplasma\u003cbr\u003eorale\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eCHO-S\u003cbr\u003e(10⁷cells\/mL)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e95.8\u003c\/td\u003e\n\u003ctd rowspan=\"4\" style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eDetection rate\u003cbr\u003eat least over\u003cbr\u003e95%.\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eMycoplasma\u003cbr\u003ehyorhinis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eMycoplasma\u003cbr\u003ehyorhinis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003eMycoplasma\u003cbr\u003efermentans\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e10\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003e100\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle; width: 16.6667%; height: 3px;\"\u003ePass\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 style=\"text-align: left;\"\u003eDocuments\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SHENTEK_flyer-AdvSHENTEKMycoplasma_Detectinnova_System20240327.pdf?v=1714461782\" type=\"application\/pdf\" target=\"_blank\"\u003eAdvSHENTEK Mycoplasma DetectInnova System_Flyer\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\u003cdiv style=\"background-color: #fffbeb; border: 1px solid #fde68a; border-radius: 8px; padding: 20px; margin-top: 30px; margin-bottom: 30px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: center; gap: 8px; margin-bottom: 12px;\"\u003e\r\n \u003csvg style=\"width: 20px; height: 20px; color: #d97706; flex-shrink: 0;\" fill=\"currentColor\" viewbox=\"0 0 20 20\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 style=\"font-weight: bold; color: #78350f; margin: 0; font-size: 16px;\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"margin-left: 28px;\"\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px; margin-bottom: 10px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n \u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv style=\"display: flex; align-items: flex-start; gap: 8px;\"\u003e\r\n \u003cspan style=\"color: #d97706; font-weight: bold; flex-shrink: 0;\"\u003e✔\u003c\/span\u003e\r\n \u003cp style=\"margin: 0; font-size: 14px; color: #374151; line-height: 1.5;\"\u003e\r\n Not intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\r\n \u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"AdvSHENTEK-4 system","offer_id":45170958729397,"sku":"1603106","price":60770.0,"currency_code":"USD","in_stock":true},{"title":"1 Sample test","offer_id":51916015796405,"sku":"1509100","price":124.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/AdvSHENTEKMycoplasmaDetectInnovaSystemSHENTEK.jpg?v=1757316072"},{"product_id":"animal-origin-residual-dna-sample-preparation-kit","title":"Animal-origin Residual DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit uses magnetic particle separation technology to extract residual DNA from a broad range of animal-derived biomaterials. The kit is designed for efficient and reproducible recovery of residual DNA from complex sample matrices including extracellular matrix, collagen and bio-absorbable membranes. Extracted DNA is compatible with downstream residual DNA quantitation by qPCR or PicoGreen assays. The kit supports manual sample preparation and automated extraction using the rHCDpurify system. Reagents are supplied for 50 extractions. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ Version\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1104193; User Guide Version: A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — residual DNA extraction from animal-derived biomaterials\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions \/ Extractions per kit\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eOverall kit shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check label for expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles storage\u003c\/td\u003e\r\n \u003ctd\u003e2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCold-stored reagents\u003c\/td\u003e\r\n \u003ctd\u003eGlycogen: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC-treated H2O\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion conditions\u003c\/td\u003e\r\n \u003ctd\u003eIncubate at 55 °C for 60 minutes (dry bath); adjust amount\/time for difficult samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (Do not add Yeast tRNA when using PicoGreen assay)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding step volumes\u003c\/td\u003e\r\n \u003ctd\u003eAfter adding binding buffer, add 200 µL isopropanol and 30 µL magnetic particles per sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL (per wash step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100–150 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 minutes, vortex intermittently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic separation time\u003c\/td\u003e\r\n \u003ctd\u003eParticle separation typically ~3–5 minutes on a magnetic stand\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomation compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with rHCDpurify automated extraction (rHCD-193 program described)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n \u003ctd\u003eNegative control sample (NCS, 1×PBS) and sample extraction recovery control (ERC) recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eEnvironmental conditions for instrument\u003c\/td\u003e\r\n \u003ctd\u003eAmbient temperature should not be lower than 22 °C for automated runs; UV sterilization recommended before\/after runs\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation:\u003c\/strong\u003e Uses magnetic particle technology for efficient nucleic acid binding and purification from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized for animal-derived biomaterials:\u003c\/strong\u003e Validated for complex matrices such as extracellular matrix, collagen and bio-absorbable membranes.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility with downstream assays:\u003c\/strong\u003e Extracted DNA is suitable for qPCR quantitation and PicoGreen analysis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports manual extraction and high-throughput automated extraction on the rHCDpurify system.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eIncluded controls and reagents:\u003c\/strong\u003e Includes reagents and recommended controls (NCS, ERC) to assess recovery and contamination.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eResidual DNA quantitation in animal-derived biomaterials\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of collagen, extracellular matrix products and bio-absorbable membranes\u003c\/li\u003e\r\n \u003cli\u003ePreclinical \/ manufacturing residual DNA assessment\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for qPCR or fluorescence-based DNA assays (PicoGreen)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) 15 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND020) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e1× PBS (NND042) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDEPC-treated H2O (NND045) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND025) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle separation-based DNA extraction (manual or automated with rHCDpurify)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read MSDS and wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 20 mL anhydrous ethanol to Wash buffer A before first use; prepare Wash buffer B as 70% ethanol with ddH2O.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution freshly; incubate samples at 55 °C for 60 min (adjust as needed).\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when using PicoGreen DNA quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly before use; avoid over-drying pellets—dry 30 s to 3 min depending on conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge after vortexing to collect liquid from tube cap and walls before magnetic separation.\u003c\/p\u003e\r\n \u003cp\u003e• Transfer eluate immediately after elution and perform downstream assays the same day to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; cold-stored reagents (e.g., glycogen) should be kept at -20 °C.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Which downstream assays are compatible with the extracted DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted DNA is compatible with qPCR-based residual DNA quantitation and fluorescence-based assays such as PicoGreen. Do not add Yeast tRNA if using PicoGreen.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended digestion protocol for samples?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution (20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC H2O per sample) and incubate at 55 °C for 60 minutes. Adjust enzyme amount and digestion time for difficult-to-digest or cross-linked samples.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should Wash buffer A be prepared before first use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 20 mL of anhydrous ethanol to Wash buffer A (NND015) before first use as instructed in the manual.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I run the kit on an automated platform?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify system (program rHCD-193). Follow the plate layout and column reagent volumes specified in the manual and perform UV sterilization as recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171044843701,"sku":"1104193","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Animal-origin_Residual_DNA_Sample_Preparation_Kit.jpg?v=1742277138"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-vaccines-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e2-8 °C \u0026amp; -20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003e\n Product Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle–based nucleic acid extraction kit specifically optimized for vaccine products. It is designed to stably and efficiently recover trace amounts of residual host-cell DNA from finished vaccines (e.g., Vero cell–derived rabies vaccines). The kit provides an integrated workflow when used with SHENTEK® host cell DNA qPCR detection kits and supports manual extraction or automated extraction using the rHCDpurify® system. Special sample processing methods can be provided for samples containing aluminum adjuvants or dextran.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct number\u003c\/td\u003e\n\u003ctd\u003eSK030206DM50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended reactions\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample input volume\u003c\/td\u003e\n\u003ctd\u003e100 µL test sample (per extraction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion reagents per sample\u003c\/td\u003e\n\u003ctd\u003e10 µL 5M NaCl, 30 µL Sample buffer (NND029), 15 µL Proteinase K (NND023)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion conditions\u003c\/td\u003e\n\u003ctd\u003eIncubate at 55 °C for 60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\n\u003ctd\u003e200 µL Binding solution (NND017) + 200 µL isopropanol + 10 µL Magnetic particles (NND030)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding procedure\u003c\/td\u003e\n\u003ctd\u003eVortex 5 minutes (medium speed); magnetic separation 3–5 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash volumes\u003c\/td\u003e\n\u003ctd\u003e700 µL Wash buffer A (with ethanol added) then 700 µL Wash buffer B (70% ethanol)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume and conditions\u003c\/td\u003e\n\u003ctd\u003e50–100 µL Elution buffer (NND019); incubate at 70 °C for 7 minutes (vortex 2–3 times during incubation)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\n\u003ctd\u003eResuspend magnetic particles before use; allow particles to separate 3–5 minutes on magnet; air-dry 1–3 minutes before elution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible automation\u003c\/td\u003e\n\u003ctd\u003erHCDpurify® automated extraction system (program rHCD-06DM50 \/ HCD-06DM50)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage stability\u003c\/td\u003e\n\u003ctd\u003eKit components stored under indicated conditions are stable up to 24 months (check label expiration)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCritical sample pH range\u003c\/td\u003e\n\u003ctd\u003epH 6.0–8.0 required; adjust with 1M HCl or 1M NaOH if outside range\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended controls\u003c\/td\u003e\n\u003ctd\u003eNegative Control Sample (NCS) and Extraction Recovery Control (ERC) (ERC spike recommended 2–10× amount found in unspiked sample)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use \/ Compliance\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — sample preparation for downstream host cell DNA qPCR assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation technology:\u003c\/strong\u003e Efficient capture and concentration of trace DNA using magnetic particles for high recovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVaccine-optimized workflow:\u003c\/strong\u003e Formulation and protocol optimized for finished vaccine matrices (e.g., Vero cell–derived rabies vaccine). Specific processing available for aluminum adjuvants or dextran-containing samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated with qPCR detection:\u003c\/strong\u003e Designed to work with SHENTEK® host cell DNA qPCR detection kits to provide a complete sample-to-result workflow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eManual and automated compatibility:\u003c\/strong\u003e Protocols for manual extraction and for automated processing on the rHCDpurify® system (96 deep-well format) to support throughput needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow-volume, trace DNA recovery:\u003c\/strong\u003e Optimized to obtain trace amounts of residual host-cell DNA stably and reproducibly from complex vaccine matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eSample pretreatment for residual host cell DNA testing in finished vaccines (e.g., Vero cell–derived rabies vaccine)\u003c\/li\u003e\n\u003cli\u003eQuality control testing during vaccine manufacture\u003c\/li\u003e\n\u003cli\u003eSample preparation prior to SHENTEK® host cell DNA qPCR assays\u003c\/li\u003e\n\u003cli\u003eAutomated high-throughput residual DNA extraction using rHCDpurify® system\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eSample buffer (NND029)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based nucleic acid extraction for downstream qPCR detection (manual or rHCDpurify automated)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Follow Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\n\u003cp\u003e• Use separate laboratory areas for negative reagent preparation, positive\/sample processing, and PCR amplification to prevent cross-contamination.\u003c\/p\u003e\n\u003cp\u003e• Ensure ambient temperature is not lower than 22 °C before starting experiments.\u003c\/p\u003e\n\u003cp\u003e• Centrifuge tubes before opening to avoid contamination; handle carefully to avoid splashing.\u003c\/p\u003e\n\u003cp\u003e• Change gloves regularly and use dedicated lab coats\/masks\/hair covers in different areas.\u003c\/p\u003e\n\u003cp\u003e• Dispose of used tips and liquid waste per regulations; do not open PCR amplification products in non-designated areas.\u003c\/p\u003e\n\u003cp\u003e• During magnetic separation, rotate tubes slowly to accelerate particle aggregation; avoid disturbing particles when removing supernatant.\u003c\/p\u003e\n\u003cp\u003e• Avoid over-drying magnetic particles after wash (over-drying can cause incomplete elution).\u003c\/p\u003e\n\u003cp\u003e• Ensure no magnetic particles or liquid remain on tube caps or walls; if present, briefly centrifuge.\u003c\/p\u003e\n\u003cp\u003e• Perform DNA detection on the same day as extraction to ensure accuracy.\u003c\/p\u003e\n\u003cp\u003e• Before automated runs, confirm 96-well plate and sleeve are secured; UV-sterilize the instrument interior and wipe with 75% ethanol before and after runs.\u003c\/p\u003e\n\u003cp\u003e• Minimum interval between two extractions on rHCDpurify® should be 30 minutes; UV sterilize instrument for at least 15 minutes between runs.\u003c\/p\u003e\n\u003cp\u003e• Collect more than 40 µL of eluate for downstream assay; transfer eluate immediately after automated run to new microcentrifuge tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is required per extraction?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eUse 100 µL test sample per extraction. The protocol specifies addition of 10 µL 5M NaCl and 30 µL Sample buffer prior to Proteinase K digestion.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the magnetic particles and Proteinase K be stored?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Store Proteinase K at -20 °C. Other buffers are stored at room temperature; kit components are stable under indicated conditions for up to 24 months (check label expiration).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit be used with automated extraction instruments?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify® automated system (program: rHCD-06DM50 \/ HCD-06DM50) using a 96 deep-well plate layout; the manual also provides program settings and plate layout guidance.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe low recovery of nucleic acids?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include missing ethanol in Wash buffer A, magnetic particles sticking to tube walls, low ionic strength, out-of-range sample pH, or incorrect storage of magnetic particles. Solutions: add ethanol to Wash buffer A per instructions, vortex\/heat to release particles from tube walls (70 °C for 2 min) and re-vortex, adjust ionic strength with 5M NaCl, adjust sample pH to neutral, and store magnetic particles at 2–8 °C (do not store at -20 °C).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171074105525,"sku":"SK030206DM50","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_Vaccines.jpg?v=1742277270"},{"product_id":"residual-pichia-pastoris-dna-quantitation-kit-shentek™","title":"Residual Pichia pastoris DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is a fluorescence-based quantitative PCR (FAM) assay designed to quantify residual Pichia pastoris host-cell DNA in biopharmaceutical materials across process stages (from in-process samples to final products). The kit includes a Pichia Pastoris DNA Control as a reference standard and provides rapid, specific quantitation with reliable sensitivity at the fg level. The kit workflow supports DNA sample dilution\/standard curve preparation, qPCR reaction mix setup and absolute quantitation using a standard curve. Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, StepOne Plus). For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Pichia pastoris DNA in biopharmaceutical samples (in-process and final products).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported quantitative range (from supplied standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 – 300 pg\/µL (equivalent to ~30 fg – 300 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReliable quantitation reported at the fg level; lowest supplied standard 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (qPCR mix 20 µL + sample 10 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003ePichia Pastoris qPCR Reaction Buffer 17 µL + Pichia Pastoris Primer\u0026amp;Probe MIX 3 µL -\u0026gt; 20 µL qPCR mix\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eProbe-based qPCR with FAM reporter dye (no quencher specified in kit instructions); use ROX as passive reference dye where applicable\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, CFX96, StepOne Plus, Linegene 9600plus, SHENTEK-96S and similar real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended. NTC Ct should be ≥ 35 cycles (or per internal validation). NCS Ct should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpike recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSpiked ERC recoveries required to be within 50% - 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ storage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stability up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003eExample workflow uses 100 µL test sample for preparation and 10 µL purified sample per qPCR reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Provides reliable quantitation at the fg level (lowest supplied standard 0.03 pg\/µL = 30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProbe-based specific detection:\u003c\/strong\u003e FAM-labeled probe assay offers high specificity for Pichia pastoris DNA fragments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard control:\u003c\/strong\u003e Includes Pichia Pastoris DNA Control for absolute quantitation and serial dilution standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated mix:\u003c\/strong\u003e Supplied reaction buffer and primer\/probe mix simplify qPCR setup and reduce preparation time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated with common real-time PCR platforms (ABI 7500, CFX96, StepOne Plus, Linegene, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow-ready for QC:\u003c\/strong\u003e Designed for in-process and final product host-cell DNA monitoring with recommended plate layouts and analysis settings.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Pichia pastoris host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and final product quality control for recombinant proteins produced in Pichia pastoris\u003c\/li\u003e\r\n \u003cli\u003eRoutine host-cell DNA monitoring during downstream purification and formulation\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike-recovery experiments for DNA residual assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris DNA Control (Part No. NNA007) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris qPCR Reaction Buffer (Part No. NNB005) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris Primer \u0026amp; Probe MIX (Part No. NNC041) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: thaw\/mix at 2–8 °C for use; unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-irradiate tabletop, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; handle primer\/probe and buffers on ice where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice and vortex to mix before use; spin briefly in microcentrifuge.\u003c\/p\u003e\r\n \u003cp\u003e• Check kit expiration date and storage conditions on labels prior to use.\u003c\/p\u003e\r\n \u003cp\u003e• Maintain recommended plate layout, replicate strategy and include NTC, NCS and ERC controls.\u003c\/p\u003e\r\n \u003cp\u003e• Spike recovery acceptance criteria: 50%–150%.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 cycles (or defined by internal validation).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit supports reliable quantitation at the fg level. The supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). Actual LOD in a given matrix should be confirmed by in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the Pichia Pastoris DNA Control and DNA Dilution Buffer (DDB) on ice. Prepare ST0 at 3000 pg\/µL then perform serial 1:10 dilutions to produce ST1–ST5: 300, 30, 3, 0.3 and 0.03 pg\/µL. Include at least five concentrations for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 95 °C for 10:00 (activation, 1 cycle), then 40 cycles of 95 °C for 00:15 (denaturation) and 60 °C for 01:00 (annealing\/extension, fluorescence read). Adjust settings if required by your instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should reagents be stored after thawing?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C per label. DNA Dilution Buffer (DDB) may be kept at 2–8 °C for short-term use; follow the instructions regarding storage of unused DDB and inspect for cloudiness\/precipitate before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171125813429,"sku":"SK030205P100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Pichia_Pastoris_DNA_Quantitation_Kit.jpg?v=1742277610"},{"product_id":"residual-hansenula-polymorpha-dna-quantitation-kit-shentek®","title":"Residual Hansenula polymorpha DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hansenula polymorpha DNA Quantitation Kit is designed to quantitatively detect and measure residual Hansenula polymorpha host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses fluorescent quantitative PCR (real-time qPCR) with a specific primer\/probe mix to provide rapid, specific and reliable quantitation at the femtogram (fg) level. The kit is supplied with DNA control material and reagents for sample dilution and qPCR reaction setup. For DNA extraction\/sample preparation procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Labeling\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ Yield\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (implied)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL to 300 pg\/µL (30 fg\/µL to 300 pg\/µL) — demonstrates femtogram-level detection sensitivity\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denature 95 °C 0:15 (40 cycles); Anneal\/Extend 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL (17 µL qPCR Reaction Buffer + 3 µL Primer\u0026amp;Probe MIX); Final reaction: 30 µL (add 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02; Automatic baseline; NTC Ct ≥ 35 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ recovery criteria\u003c\/td\u003e\r\n \u003ctd\u003eERC recovery acceptable range: 50%–150%; NCS Ct should be larger than mean Ct of lowest standard curve concentration (or less than LOQ if LOQ \u0026lt; lowest standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C; kit components stable for up to 24 months (check expiration on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Mx3000P, StepOne Plus, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual Hansenula polymorpha DNA at femtogram-level sensitivity (standard curve includes down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFluorescent real-time qPCR:\u003c\/strong\u003e Uses a validated primer \u0026amp; probe mix with FAM reporter for specific and rapid quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for quantitation:\u003c\/strong\u003e Includes DNA control, qPCR reaction buffer, primer \u0026amp; probe mix and DNA dilution buffer — sufficient reagents for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Protocols and settings provided for commonly used real-time PCR platforms; assay template provided for absolute quantitation (standard curve).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC and controls included:\u003c\/strong\u003e Guidance and materials for NTC, NCS and ERC controls; recommended acceptance criteria (NTC Ct ≥35; ERC recovery 50–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha DNA in final drug products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC testing for host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eResearch applications involving detection of Hansenula polymorpha DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha DNA Control (NNA008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha qPCR Reaction Buffer (NNB007)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 μL × 2 tubes) — protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha Primer \u0026amp; Probe MIX (NNC040)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate working surfaces, pipettes and tubes (UV irradiation 30 minutes recommended) and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C; check expiration dates.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer is cloudy, heat to 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (NTC, NCS, ERC) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Recommended qPCR analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35 cycles or undetermined; ERC recovery 50%–150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level detection; the provided standard curve covers 0.03 pg\/µL (30 fg\/µL) to 300 pg\/µL. Users should validate LOD\/LOQ in their own laboratory and instrument setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR program and settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 0:15 (denature) and 60 °C 1:00 (anneal\/extend, read fluorescence). Analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline. Adjust for your instrument if required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions of the provided DNA Control in DNA Dilution Buffer to generate at least five concentrations (example: 300, 30, 3, 0.3, 0.03 pg\/µL). Run each standard in triplicate for absolute quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are acceptable QC criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or Ct ≥ 35 cycles. NCS Ct should be larger than the mean Ct of the lowest standard or below the LOQ if LOQ is lower than the lowest standard. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171150553269,"sku":"SK030222HA100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hansenula_Polymorpha_DNA_Quantitation_Kit.jpg?v=1742277725"},{"product_id":"residual-saccharomyces-cerevisiae-dna-quantitation-kit-shentek®","title":"Residual Saccharomyces¬†cerevisiae DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Saccharomyces cerevisiae DNA Quantitation Kit is designed to quantify residual Saccharomyces cerevisiae DNA in biopharmaceutical materials across process stages from in-process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with analytical sensitivity at the femtogram (fg) level. For DNA extraction guidance, refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit supplies reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking standard range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standards prepared from DNA control: 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest standard (example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL (30 fg\/µL) included as lowest standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (document states fg-level sensitivity); practical detection guided by lowest standard and lab validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified; users should determine LOQ by laboratory validation. The lowest provided standard is 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR master mix per reaction 20 µL + 10 µL sample = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMaster mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eSaccharomyces cerevisiae qPCR Reaction Buffer 17 µL + Primer \u0026amp; Probe MIX 3 µL (total 20 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C 10:00 (1 cycle); Denature: 95 °C 00:15 (40 cycles); Anneal\/Extend: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dye \/ probe\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; no quencher specified in instructions (primer \u0026amp; probe mix provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold \/ analysis settings\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended Manual Ct threshold = 0.02; baseline = Automatic. NTC Ct should be ≥ 35.00 cycles or undetermined (or laboratory-specific criteria).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIncludes DNA control for standard curve, Negative Control Sample (NCS), No Template Control (NTC) and Extraction Reference Control (ERC). ERC recovery acceptance: 50%–150%. Ct(NCS) should be larger than mean Ct of lowest standard (or below LOQ if LOQ proven lower).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, samples and controls\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months when stored appropriately (see component-level storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended consumables\u003c\/td\u003e\r\n \u003ctd\u003eDNase-free low-retention tubes and tips; 96-well qPCR plates with sealing film or 8-strip PCR tubes with caps\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to detect Saccharomyces cerevisiae DNA down to the femtogram (fg) level; includes low-concentration standards (down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and reliable:\u003c\/strong\u003e Contains optimized primer \u0026amp; probe mix and qPCR reaction buffer for specific amplification and reproducible quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Supplies DNA control, qPCR reaction buffer and primer \u0026amp; probe mix to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated for use on multiple real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit components are stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eHost cell DNA residue testing during upstream\/downstream process development and release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike\/recovery experiments using ERC samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae DNA Control (Part No. NNA009) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae qPCR Reaction Buffer (Part No. NNB006) — 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae Primer \u0026amp; Probe MIX (Part No. NNC039) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and change tips frequently.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex to mix and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial dilution as instructed; include at least five concentrations for the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Triplicate measurements are recommended for standards, samples and controls.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35 or be undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery should be between 50% and 150%; verify extraction efficiency and assay performance.\u003c\/p\u003e\r\n \u003cp\u003e• Determine LOQ and assay validation parameters within your laboratory according to regulatory guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Agilent Mx3000PTM and StepOne Plus. If using other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the lowest standard concentration provided and does it represent the kit LOQ?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest provided standard is 0.03 pg\/µL (30 fg\/µL). The kit states fg-level sensitivity, but you should determine the limit of quantification (LOQ) for your matrix by laboratory validation; the provided lowest standard can be used as a reference point.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I handle and store unused DNA Dilution Buffer (DDB)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore remaining unused DDB at 2–8 °C. If the solution becomes cloudy or precipitated, warm to 37 °C until clear before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be ≥ 35 cycles or undetermined. Ct of NCS should be larger than the mean Ct of the lowest standard (or below LOQ if the LOQ is proven lower than the lowest standard). ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171176636597,"sku":"1101103","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Saccharomyces_Cerevisiae_DNA_Quantitation_Kit.jpg?v=1742277789"},{"product_id":"residual-cho-dna-quantitation-kit-shentek®","title":"Residual CHO DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit is designed for quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (absolute quantitation with a standard curve) to perform rapid and specific quantitation of residual DNA at the femtogram level. For nucleic acid extraction and sample preparation guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100). The kit is supplied as reagents for 100 reactions and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical products (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by quantitative PCR (standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard concentrations prepared from CHO DNA Control: ST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL; ST6 = 0.003 pg\/μL. (Implied dynamic range: 300 to 0.003 pg\/μL using provided standards.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eStandards include down to 0.003 pg\/μL (ST6). No explicit LOQ\/LOD numeric claim provided; users should validate limit of quantitation (QL) in their own laboratory.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or standard).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample (or 10 μL standard\/controls as described).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix provided\u003c\/td\u003e\r\n \u003ctd\u003eReady-to-use CHO qPCR MIX supplied (mix thoroughly before use); protect from light.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C for 10:00; 40 cycles of Denaturation 95 °C for 0:15 and Annealing\/Extension 60 °C for 1:00 (fluorescence read at 60 °C).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling count\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — ERC recovery\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecovery of Extraction Reference Control (ERC) samples should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — NTC \/ NCS\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct should be Undetermined or not less than 35.00 (or laboratory-specific criteria). NCS Ct should be larger than the mean Ct of the lowest standard; if proven QL is lower than minimum standard concentration, NCS detected value should be less than QL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 Plus, Mx3000P, qTOWER 3 G, StepOnePlus (consult instrument documentation for setup).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components may be stored under recommended conditions for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended analysis settings (example ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set task types for standard, unknown, NTC as described.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of detecting residual CHO DNA down to femtogram-level concentrations (standards provided to 0.003 pg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Provided CHO qPCR MIX formulated for absolute quantitation—minimizes preparation steps and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard curve control:\u003c\/strong\u003e Includes CHO DNA Control and DNA Dilution Buffer to prepare a 6-point standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR platforms; instrument-specific setup instructions included.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow guidance:\u003c\/strong\u003e User guide includes serial dilution protocol, plate layout examples, thermal cycling program, and acceptance criteria (ERC recovery, NTC\/NCS rules).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics production (host cell DNA monitoring)\u003c\/li\u003e\r\n \u003cli\u003eProcess development and purification monitoring for cell-derived products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing in research settings\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (Part No. NNA001)\u003c\/span\u003e\u003cstrong\u003e50 μL × 1 tube; store at –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO qPCR MIX (Part No. NNC001)\u003c\/span\u003e\u003cstrong\u003e1 mL × 2 tubes; store at –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL × 3 tubes; store at –20 °C (remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (absolute quantitation, standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eye protection, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and centrifuge briefly; mix CHO qPCR MIX thoroughly and protect from light.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific setup and analysis parameters; example settings are provided for ABI 7500 SDS v1.4 but may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at recommended temperatures; per USP \u0026lt;659\u0026gt; items with –20 °C recommendation should be stored within ±10 °C (–30 °C to –10 °C).\u003c\/p\u003e\r\n \u003cp\u003e• Validate limit of quantitation (QL) and other performance metrics in your laboratory prior to routine testing.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure NTC (no template control) is undetermined or Ct ≥ 35 and NCS meets acceptance criteria before reporting sample results.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity of the kit and how low can it quantify CHO DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve includes concentrations down to 0.003 pg\/μL (ST6). The kit enables femtogram-level detection, but users should perform laboratory validation to determine the assay’s limit of quantitation (QL) under their specific conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL CHO qPCR MIX + 10 μL sample or standard). Use 10 μL of purified test sample per reaction as described in the user guide.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should reagents be stored and what is kit stability?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore CHO DNA Control, CHO qPCR MIX and DNA Dilution Buffer at –20 °C (protect qPCR mix from light). Kit components are stable for up to 24 months; check expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC shows amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC (no template control) is amplified (Ct \u0026lt; 35 or determined), suspect contamination. Decontaminate workspace and consumables (UV, 75% ethanol), re-prepare master mixes and controls, and repeat the assay. Verify pipetting technique and use fresh aliquots of reagents.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171252527285,"sku":"SK030201C100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit.jpg?v=1742278019"},{"product_id":"residual-ns0-sp2-0-dna-quantitation-kit-shentek®","title":"Residual NS0 \u0026 SP2\/0 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual NS0\u0026amp;SP2\/0 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical matrices (in-process samples to final products). The kit provides NS0\u0026amp;SP2\/0 DNA Control as a reference standard and contains qPCR Reaction Buffer, NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX, and DNA Dilution Buffer. The assay is designed for sensitive, specific and rapid quantitation at the femtogram (fg) level. Reagents in this kit are sufficient for 100 reactions. For DNA extraction procedures refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR using a supplied standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; lowest kit standard = 0.003 pg\/µL (3 fg\/µL). Laboratory validation required to establish LOD\/LOQ.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL (ST0 stock: 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX 3 µL = 20 µL per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (data acquisition during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, Bio-Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥ 35 cycles or undetermined (or per lab validation); Ct of NCS should be \u0026gt; mean Ct of lowest standard or below validated LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable for up to 24 months when stored as instructed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReference \/ related product\u003c\/td\u003e\r\n \u003ctd\u003eFor extraction information see SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at femtogram (fg) level; lowest supplied standard 0.003 pg\/µL\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Supplies DNA control and primer\/probe mix for construction of standard curve and absolute quantitation\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX minimize assay setup variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use on multiple common real-time PCR platforms\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuality controls included:\u003c\/strong\u003e Includes NS0\u0026amp;SP2\/0 DNA Control for standard curve and guidance for NTC, NCS and ERC controls\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual NS0 host cell DNA in biopharmaceutical process intermediates and final drug substance\/formulation\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual SP2\/0 host cell DNA in biopharmaceutical samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA testing in QC and R\u0026amp;D (Research Use Only)\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 DNA Control (Part No. NNA021)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 µL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX (Part No. NNC029)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes (remaining unused DDB store 2–8 °C; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory clothing and gloves\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and dedicated workspace\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate workspace, pipettes and tubes with UV for 30 minutes and 75% ethanol before setup\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly prior to use\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates\/strips and spin briefly before running qPCR\u003c\/p\u003e\r\n \u003cp\u003e• Establish laboratory-specific LOQ\/LOD by validation; NTC Ct acceptance: ≥ 35 cycles or undetermined\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance: 50%–150%\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration standard provided in the kit and what sensitivity can I expect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest supplied standard (ST6) is 0.003 pg\/µL (3 fg\/µL). The kit is designed to enable quantitation at the femtogram level; however, actual limit of detection (LOD) and limit of quantitation (LOQ) should be established by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the NS0\u0026amp;SP2\/0 DNA Control and DNA Dilution Buffer at 2–8 °C. Dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to prepare ST1–ST6 (ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL). Include at least five concentrations on the standard curve for quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What qPCR cycling conditions and reaction setup should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction volume (20 µL qPCR mix + 10 µL sample). Cycling: Activation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 0:15 (40 cycles); Annealing\/Extension 60 °C for 1:00 with fluorescence read during this step. Prepare qPCR mix per reaction: 17 µL qPCR Reaction Buffer + 3 µL Primer \u0026amp; Probe MIX.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I interpret controls and QC criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35 (or per lab validation). NCS Ct should be larger than mean Ct of lowest standard in the curve or below validated LOQ. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171267436725,"sku":"SK030208N100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_NS0_SP2_0_DNA_Quantitation_Kit.jpg?v=1742278378"},{"product_id":"residual-vero-dna-154-quantitation-kit-shentek®","title":"Residual Vero DNA-154 Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA-154 Quantitation Kit is a research-use qPCR kit for quantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process to final products). The kit uses a fluorescent quantitative PCR (FAM reporter) assay with a pre-formulated primer \u0026amp; probe mix and Vero DNA Control standard to enable rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit supplies Vero DNA Control as a reference standard and DNA Dilution Buffer for standard curve preparation. For nucleic acid extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (TaqMan-style probe); absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (documented serial dilutions down to 0.0003 pg\/μL = 0.3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003ePrepared from Vero DNA Control: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction (20 μL qPCR MIX + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + Vero Primer \u0026amp; Probe MIX-154 3 μL = 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReporter \/ Reference dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR instrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus (and similar real-time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTriplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality criteria \/ acceptance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate should be 50%–150%; Ct of NTC should be ≥35 or undetermined; Ct of NCS should be larger than mean Ct of lowest standard (or below validated LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months when stored as instructed (-20 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating Vero host DNA down to the femtogram level using an optimized primer\/probe assay and standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes pre-formulated Vero Primer \u0026amp; Probe MIX-154 for consistent, specific detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSupplied reference standard:\u003c\/strong\u003e Vero DNA Control provided for preparation of an 8-point serial dilution standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with multiple qPCR platforms:\u003c\/strong\u003e Validated workflows and example settings included for several common real-time PCR instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eUser guidance for QC:\u003c\/strong\u003e Includes instructions for NTC, NCS, ERC controls and acceptance criteria (ERC recovery 50%–150%, NTC Ct ≥35).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of drug substance and drug product for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of downstream purification steps\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-154 (NNC018)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 μL × 1 tube; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance range: 50%–150%; ensure NTC Ct ≥35 or undetermined.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample). qPCR MIX per reaction is composed of 17 μL qPCR Reaction Buffer + 3 μL Vero Primer \u0026amp; Probe MIX-154.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve concentrations should I prepare?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the provided Vero DNA Control as follows: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL. Include at least five concentration points in the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be within 50%–150%. The Ct for NTC should be ≥35 or undetermined. The Ct for NCS should be greater than the mean Ct of the lowest standard (or below validated LOQ if LOQ is lower than the lowest standard).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus, and similar instruments. Adjust analysis settings to your instrument\/software as needed.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171280609461,"sku":"SK030227V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA-154_Quantitation_Kit.jpg?v=1742278786"},{"product_id":"residual-mdck-dna-quantitation-kit-shentek®","title":"Residual MDCK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Quantitation Kit is designed to quantitate residual MDCK host cell DNA in biopharmaceutical workflows, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) for rapid, specific and reliable absolute quantitation at the femtogram (fg) level. The kit includes a MDCK DNA control, primer \u0026amp; probe mix, reaction buffer and DNA dilution buffer. For nucleic acid extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical samples (in‑process and final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative PCR (qPCR), absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims detection at femtogram (fg) level; lowest provided standard concentration = 0.003 pg\/μL (3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Linear range (provided)\u003c\/td\u003e\r\n \u003ctd\u003eStandard concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03, 0.003 pg\/μL (ST0–ST6); at least five concentrations recommended for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Quantitation (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eLOQ not explicitly specified; protocol notes use of validated LOQ; if LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal qPCR reaction volume = 30 μL (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + MDCK Primer \u0026amp; Probe MIX 3 μL = 20 μL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real‑time PCR instruments (SHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; Negative Control Sample (NCS) Ct should be larger than mean Ct of lowest standard (or less than LOQ if validated LOQ is lower); ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as labeled Product No.: SK030209M100)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components storable under appropriate conditions up to 24 months; individual components stored at -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay readout \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eRecommended analysis: Manual Ct, threshold for MDCK‑DNA = 0.02, automatic baseline; set tasks (Standard, Unknown, NTC) and verify slope, intercept, R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual MDCK DNA down to the femtogram level (lowest provided standard 0.003 pg\/μL = 3 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Standard‑curve based absolute quantitation using supplied MDCK DNA control and serial dilutions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes validated MDCK Primer \u0026amp; Probe MIX to simplify assay setup and improve reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on multiple common real‑time PCR platforms (ABI 7500, CFX96, StepOne Plus, LineGene, Mx3000P, SHENTEK‑96S, etc.).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eKit format:\u003c\/strong\u003e Provides reagents for 100 reactions with pre‑measured components (control, buffer, primer\/probe mix and dilution buffer).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process development\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support for final biopharmaceutical products (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for MDCK host cell DNA detection\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) — 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX (NNC024) — 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or therapeutic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low‑retention consumables; decontaminate workspace and pipettes with UV (30 min) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly; store unused DNA Dilution Buffer at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light‑sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, ERC and standard curve in each run.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument\/software‑specific parameter settings; example settings provided for ABI 7500 with SDS v1.4.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest standard concentration provided and what is the claimed sensitivity?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided serial dilution scheme includes standards down to 0.003 pg\/μL (3 fg\/μL). The kit is intended to enable quantitation at the femtogram level.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 20 μL qPCR mix per reaction (17 μL qPCR Reaction Buffer + 3 μL Primer \u0026amp; Probe MIX) and add 10 μL sample\/standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample program: Activation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15 (40 cycles); Annealing\/extension 60 °C 1:00 with fluorescence read during this step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should be used for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than the mean Ct of the lowest standard (or below validated LOQ if applicable); ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171293880501,"sku":"SK030209M100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Quantitation_Kit.jpg?v=1742279376"},{"product_id":"residual-sf9-acnpv-dna-quantitation-kit-shentek®","title":"Residual Sf9 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9\u0026amp;AcNPV DNA Quantitation Kit is designed for quantitative measurement of residual Sf9 and AcNPV host cell DNA in biopharmaceutical materials at different processing stages (in-process to final products). The kit uses a multiplex quantitative PCR (qPCR) assay with internal positive control (IPC) to provide rapid, specific and reliable quantitation down to femtogram-level sensitivity. Supplied reagents include DNA control standards for Sf9 and AcNPV, primer\/probe mixes, IPC mix, qPCR reaction buffer and DNA dilution buffer. The kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A). Refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction guidance.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (Sf9)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e300 pg\/µL to 0.003 pg\/µL (standard curve points: 300, 30, 3, 0.3, 0.03, 0.003 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (AcNPV)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5.14×10^6 to 5.14×10^1 copies\/µL (equivalent mass: 20 to 0.0002 pg\/µL; standard curve points provided for both copies\/µL and pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity down to femtogram (fg) level implied by standard curve lowest points (Sf9: 0.003 pg\/µL = 3 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReaction total volume 30 µL per well (20 µL qPCR MIX + 10 µL sample).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL (total 20 µL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C for 10:00 (1 cycle); Denaturation: 95 °C for 0:15; Annealing\/Extension: 60 °C for 1:00 (read fluorescence). 40 cycles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument channels \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eSf9-DNA: FAM; AcNPV-DNA: CY5; IPC: VIC; Passive reference dye: ROX (ABI 7500 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ quantitation settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold 0.02 for Sf9-DNA, AcNPV-DNA and IPC; use Absolute Quantitation (Standard Curve) analysis.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC: Ct(sample) should be within ±1.0 Ct of Ct(NCS) — large deviation indicates inhibition. NTC: Ct ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as shipped).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see component-specific storage: most components -20 °C; protect qPCR Reaction Buffer from light).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ baseline guidance\u003c\/td\u003e\r\n \u003ctd\u003eThreshold set to 0.02; Automatic baseline recommended when using provided settings.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR:\u003c\/strong\u003e Simultaneous detection and quantitation of Sf9-DNA, AcNPV-DNA and IPC in one reaction using distinct fluorescent channels (FAM, CY5, VIC).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay standard curve includes femtogram-level concentrations (Sf9 down to 0.003 pg\/µL) enabling detection at fg scale.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC provided to monitor PCR inhibition and overall reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix, IPC mix and reaction buffer simplify setup and reduce pipetting errors.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated examples and guidance provided for multiple real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, CFX96, FQD-96A).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStandardized quantitation:\u003c\/strong\u003e Supplied Sf9 \u0026amp; AcNPV DNA controls with serial dilution scheme for generating absolute standard curves (copies\/µL and pg\/µL).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host cell DNA in biopharmaceutical process and final drug substance\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Autographa californica multiple nucleopolyhedrovirus (AcNPV) DNA in bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control and release testing for products produced in Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV DNA Control (NNA051), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX (NNC059), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC067), 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused remaining DDB may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free area; use DNase-free, low-retention consumables and tips.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate work surfaces and equipment for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial 10-fold dilutions in DDB as instructed; at least five standard concentrations recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates well, mix and spin down prior to starting qPCR to avoid evaporation and inconsistent Ct values.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC: sample Ct-IPC should be within ±1.0 Ct of NCS; significant increase indicates sample inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35.00 or be undetermined; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents for 100 reactions (stated on the product cover).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and thermal program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 30 µL reactions (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 with fluorescence read during the 60 °C step. Use manual Ct threshold 0.02 as guidance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than Ct-IPC of NCS (beyond ±1.0 Ct), consider sample dilution, re-extraction, or purification to remove inhibitors. Use recovery of spiked reference standards to assess sample processing.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the assay dynamic ranges and units?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSf9 standard curve: 300 to 0.003 pg\/µL. AcNPV standard curve: 5.14×10^6 to 5.14×10^1 copies\/µL (equivalent to 20 to 0.0002 pg\/µL). At least five standard concentrations should be used for curve generation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171362431157,"sku":"1101101","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_AcNPV_DNA_Quantitation_Kit.jpg?v=1742279477"},{"product_id":"residual-cv-1-dna-quantitation-kit-shentek-n","title":"Residual CV-1 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CV-1 DNA Quantitation Kit is designed to quantitate residual CV-1 host cell DNA across stages of biopharmaceutical production, from in-process samples to final products. The kit uses quantitative PCR (qPCR) to deliver rapid, specific and reliable quantitation at the femtogram (fg) level and includes a CV-1 DNA Control reference standard. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST0: 3000 pg\/µL; ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest included standard (indicative sensitivity)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.003 pg\/µL (3 fg\/µL) — kit supports femtogram-level quantitation; validated LOQ must be determined by laboratory validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (20 µL qPCR mix + 10 µL sample\/diluent)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer: 17 µL; CV-1 Primer \u0026amp; Probe MIX: 3 µL; Total qPCR MIX per reaction: 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C, 10:00 (1 cycle); Denaturation: 95 °C, 00:15 (40 cycles); Annealing\/Extension: 60 °C, 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection settings\u003c\/td\u003e\r\n \u003ctd\u003eRecommended reporter dye: FAM; passive reference: ROX (ABI7500 example). Manual Ct threshold example: 0.02 (Automatic baseline recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 cycles or undetermined (or per laboratory validation); NCS Ct should be larger than mean Ct of lowest standard (or below LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months at recommended storage conditions (component-specific temperatures listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims femtogram-level detection; lowest provided standard 0.003 pg\/µL (3 fg\/µL) allowing very low-level residual DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Absolute quantitation using a supplied CV-1 DNA Control standard curve (six dilution points recommended).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX to streamline assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComprehensive workflow support:\u003c\/strong\u003e Includes guidance for standard curve preparation, plate layout recommendations, qPCR program settings and result analysis instructions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in final drug substance\/drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in CV-1 cells\u003c\/li\u003e\r\n \u003cli\u003eProcess development and optimization where host-cell DNA monitoring is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 DNA Control (NNA026)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 Primer \u0026amp; Probe MIX (NNC097)\u003c\/span\u003e\u003cstrong\u003eStore as labeled (provided 300 μL × 1 tube); follow kit storage instructions\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C during use); 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation\u003c\/span\u003e\u003cstrong\u003eUser Guide and instructions included\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for human diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and pipettes with UV (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice (2–8 °C), vortex and spin briefly before use; protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh using serial dilutions; include at least five concentrations for curve fitting.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates with appropriate film, mix and spin briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC Ct ≥35 cycles or undetermined (or per lab validation); NCS Ct must be \u0026gt; mean Ct of lowest standard or below validated LOQ.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific program and detector settings must be configured per instrument\/software documentation (ABI7500 example provided).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity or lowest detectable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides a lowest standard of 0.003 pg\/µL (3 fg\/µL) and is designed to support femtogram-level quantitation. The validated limit of quantitation (LOQ) should be determined by laboratory validation and may be lower or higher depending on sample matrix and extraction recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should reagents be stored and handled?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures (major components at -20 °C). Thaw reagents on ice (2–8 °C), vortex and spin briefly before use. Protect light-sensitive components (e.g., qPCR Reaction Buffer) from light. Follow MSDS guidance and use DNase-free consumables.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should have Ct ≥35 cycles or be undetermined (or per laboratory validation). The NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve or below the validated LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are supported and what detector settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). In the ABI7500 example, create a FAM detector (reporter FAM), set ROX as passive reference, use Manual Ct threshold 0.02 and Automatic baseline; thermal cycling: 95 °C activation 10:00; 40 cycles of 95 °C 00:15 and 60 °C 1:00 (read fluorescence during anneal\/extension). Adjust settings per instrument and software version.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187376840885,"sku":"1101105","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CV-1_DNA_Quantitation_Kit.webp?v=1742279215"},{"product_id":"residual-hi5-acnpv-dna-quantitation-kit-shentek®","title":"Residual Hi5 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n 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8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hi5\u0026amp;AcNPV DNA Quantitation Kit is designed to quantify residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses a multiplex quantitative PCR (qPCR) assay with target-specific primer\/probe mixes for Hi5 DNA and AcNPV DNA plus an Internal Positive Control (IPC) to monitor reaction performance and potential inhibition. The assay supports absolute quantitation using a supplied lyophilized DNA control and DDB-based serial dilutions. The kit provides reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples across process stages\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMultiplex quantitative PCR (qPCR) with three detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 μL total per well (20 μL qPCR mix + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; Hi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eHi5 DNA standard curve (mass)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points: 30 pg\/μL (ST1), 3 pg\/μL (ST2), 0.3 pg\/μL (ST3), 0.03 pg\/μL (ST4), 0.003 pg\/μL (ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcNPV DNA standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points (copies\/μL and pg\/μL): ST1 5.14×10^5 copies\/μL (2 pg\/μL), ST2 5.14×10^4 (0.2 pg\/μL), ST3 5.14×10^3 (0.02 pg\/μL), ST4 5.14×10^2 (0.002 pg\/μL), ST5 5.14×10^1 (0.0002 pg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity claim\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level is supported by the provided standard curve down to 0.003 pg\/μL for Hi5 and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC Ct of sample vs NCS should be within ±1.0 Ct; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger (i.e., later) than mean Ct of lowest standard; NCS should show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample (per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable up to 24 months when stored as directed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided quantity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions (kit contains reagents sized as listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated LOQ is referenced in method validation; the user should determine LOQ during method validation. If LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR format:\u003c\/strong\u003e Simultaneous detection of Hi5 DNA, AcNPV DNA and IPC in a single reaction (FAM, CY5, VIC channels)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Supports quantitation down to femtogram-level concentrations using supplied standards (mass and copy-number options for AcNPV)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify inhibited samples\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer\u0026amp;Probe MIX and IPC MIX reduce assay setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hi5 host-cell DNA in biopharmaceutical process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual AcNPV DNA (baculovirus) in upstream and downstream process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support and method development for biologics produced in Hi5\/AcNPV systems\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV DNA Control (lyophilized powder) ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C (after opening\/for use, store remaining DDB at 2–8 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, mask, protective eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout recommendations and include at least five standard curve points, NTC and NCS in each run.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: Ct(sample IPC) must be within ±1.0 Ct of Ct(NCS) — higher IPC Ct indicates potential inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: Ct ≥ 35 or undetermined; NTC should not show target amplification.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Validated LOQ must be established during method validation; if LOQ is below the lowest standard, ensure NCS value is below LOQ.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration for Hi5 and AcNPV using this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve supports quantitation down to 0.003 pg\/μL for Hi5 DNA and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV DNA. Users should perform method validation to determine assay LOD\/LOQ for their matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample should be added per reaction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 10 μL of purified test sample per 30 μL reaction (20 μL qPCR mix + 10 μL sample).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the instrument settings for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal profile: Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 01:00 with fluorescence read at 60 °C. Set reaction volume to 30 μL. Configure detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC Ct is significantly higher than NCS IPC Ct (outside ±1.0 Ct), suspect inhibition. Consider diluting the sample, re-extracting with improved purification, or including spike-recovery controls to assess sample recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173391065269,"sku":"1101102","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hi5_AcNPV_DNA_Quantitation_Kit.png?v=1742279579"},{"product_id":"residual-human-dna-quantitation-kit-shentek®","title":"Residual Human DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Human DNA Quantitation Kit (2G) is a duplex quantitative PCR (qPCR) kit for quantitation of residual human host-cell DNA in biopharmaceutical samples from in-process to final products. The assay achieves femtogram-level sensitivity and includes an Internal Positive Control (IPC) in the Human Primer \u0026amp; Probe MIX to monitor PCR performance. The kit contains a Human DNA Control for standard curve generation and ready-to-use qPCR Master MIX and Primer\u0026amp;Probe MIX. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual human host-cell DNA in biopharmaceutical samples (in-process to final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex quantitative PCR (qPCR) with Human-DNA detector (FAM) and IPC (VIC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level; NTC detection value acceptance ≤ 6.00 fg\/μL (user may set criteria by method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical linear range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e300 pg\/μL to 0.03 pg\/μL (example standard series provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis thresholds (example)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-DNA = 0.05; IPC = 0.1; Automatic baseline\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate 50%–150%; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification; NTC detection ≤ 6.00 fg\/μL and IPC amplification normal\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePack size \/ yield\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions (product no. 1101108)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored at appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation at femtogram (fg) level\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes Internal Positive Control in the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and Primer\u0026amp;Probe MIX reduce setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR systems (SHENTEK-96S, ABI 7500, CFX96, LightCycler 480)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow convenience:\u003c\/strong\u003e Includes Human DNA Control and DNA Dilution Buffer with recommended serial dilution scheme for standard curve generation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human host-cell DNA in biopharmaceutical products (in-process and final product)\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring and release testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eComparative testing of extraction methods (use with Extraction Reference Control, ERC)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer \u0026amp; Probe MIX (includes IPC) (NNC130) 500 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReagents for 100 reactions (kit pack information)\u003c\/span\u003e\u003cstrong\u003e-20 °C (components stable up to 24 months)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination. UV decontaminate work surfaces and pipettes prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Master MIX from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, and ERC. Run standards (at least five concentrations) to build a standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC ≤ 6.00 fg\/μL (or criteria defined during method validation); NCS Ct larger than mean Ct of lowest standard and shows IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR analysis parameters (e.g., thresholds, baseline) should be configured for the specific instrument and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level quantitation. Example standard points include down to 0.03 pg\/μL (30 fg\/μL). The kit documentation states NTC detection acceptance ≤ 6.00 fg\/μL; true LOD\/LOQ should be determined by the user's method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Roche LightCycler 480. It is compatible with other real-time PCR instruments that support dual-channel detection (FAM and VIC), but instrument-specific setup is required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How many reactions are included and how should reagents be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Store components at -20 °C and protect the qPCR Master MIX from light. Components are stable up to 24 months—confirm expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I run and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRun a standard curve (minimum five points), Non-Template Control (NTC), Negative Control Sample (NCS), and Extraction Reference Control (ERC). ERC recovery should be between 50% and 150%. NTC detection should be ≤ 6.00 fg\/μL (or per user validation). NCS Ct should be larger than the mean Ct of the lowest standard and show IPC amplification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173398765749,"sku":"1101108","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Quantitation_Kit.png?v=1742279705"},{"product_id":"residual-bhk-dna-quantitation-kit-shentek®","title":"Residual BHK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Quantitation Kit is designed to quantitate residual BHK host cell DNA in different stages of biopharmaceutical products, from in‑process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit includes a BHK DNA Control and reagents for preparing standard curves and performing absolute quantitation. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical samples (in‑process to final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by fluorescent qPCR (standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects at the femtogram (fg) level (laboratory validation recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e3000 pg\/µL, 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (ST0–ST5 as prepared per protocol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + BHK Primer\u0026amp;Probe MIX 3 µL → 20 µL qPCR mix; add 10 µL sample\/standard to reach 30 µL total\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: none (per setup instructions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard (or larger than LOQ Ct if LOQ is below lowest standard); ERC recovery rate should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; stable up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reaction count\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents supplied for 100 reactions (kit format)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating residual BHK DNA at the femtogram (fg) level when properly validated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes BHK DNA Control and standard curve workflow for absolute quantitation of target DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Contains qPCR Reaction Buffer and Primer \u0026amp; Probe MIX optimized for the assay to reduce setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated for use on SHENTEK‑96S, ABI 7500, Roche LightCycler 480 II and other real‑time PCR systems (instrument settings may vary).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Intended for testing residual host cell DNA across in‑process samples to final product stages and for assay validation workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing for residual DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003cli\u003eQuality control and stability studies involving host cell DNA measurements\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (Part No. NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX (Part No. NNC076) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low retention consumables and clean work area (UV irradiation and 75% ethanol decontamination recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly spin before use; protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration date on the label.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points for the standard curve and perform triplicates for samples and controls where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery rates should be between 50% and 150%; investigate deviations.\u003c\/p\u003e\r\n \u003cp\u003e• Set instrument analysis parameters (thresholds, baseline, detector settings) according to instrument\/software and laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to enable quantitation at the femtogram (fg) level. The practical limit of detection (LOD) and limit of quantification (LOQ) should be established by laboratory validation. The provided standard curve includes concentrations down to 0.03 pg\/µL (30 fg\/µL as prepared).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real‑time PCR instruments. The user guide provides example settings for SHENTEK‑96S, ABI 7500 (SDS v1.4), and Roche LightCycler 480 II. Adjust instrument and software parameters as needed for other platforms and validate performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the BHK DNA Control and DNA Dilution Buffer at 2–8 °C, dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentration points and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC or NCS show amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC shows amplification (Ct \u0026lt; 35), verify reagent and plate handling to identify contamination sources, replace consumables, re‑prepare reagents, decontaminate work surfaces and equipment (UV\/ethanol), and repeat the assay. If NCS Ct is not larger than the lowest standard Ct or LOQ Ct, review extraction and dilution procedures and revalidate the LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173411610805,"sku":"1101116","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Quantitation_Kit.png?v=1742279915"},{"product_id":"residual-e1a-dna-quantitation-kit-shentek®","title":"Residual E1A DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1A DNA Quantitation Kit is designed for the quantitation of residual E1A DNA derived from host cells (e.g., HEK293 and 293T) across different stages of biopharmaceutical manufacture, from in-process samples to final products. The kit uses fluorescent quantitative PCR (real-time qPCR) for rapid, specific and reliable absolute quantitation. An Internal Positive Control (IPC) is included to monitor PCR performance and potential inhibition. The kit provides both E1A linear DNA Control and E1A non-linear DNA Control to accommodate different user needs. Recommended sample extraction procedures are provided in the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e4.97 × 10^8 copies\/μL (stock) with serial 10-fold dilutions down to 4.97 × 10^1 copies\/μL (ST1–ST6 covering 4.97 × 10^6 to 4.97 × 10^1 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (non-linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e2.98 × 10^7 copies\/μL (stock) with serial 10-fold dilutions down to 2.98 × 10^2 copies\/μL (ST0–ST5 covering 2.98 × 10^6 to 2.98 × 10^2 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; E1A Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample or purified NCS per reaction; recommended starting material for extraction: 100 μL sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection dyes\u003c\/td\u003e\r\n \u003ctd\u003eE1A-DNA detector: CY5; IPC detector: VIC; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for both E1A-DNA and IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt value of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; significant shift indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ \/ LOD\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ) not specified in kit documentation; users should determine proven LOQ during method validation. If proven LOQ is lower than lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended replicates and standard points\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended; at least five concentration points recommended for standard curve (six when using linear DNA control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eValidated instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other compatible real-time PCR systems may be used)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and sensitive absolute quantitation:\u003c\/strong\u003e Fluorescent qPCR assay for quantitative detection of residual E1A DNA with serial standards to create an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify potential sample inhibition; IPC results used to assess recovery when spiked samples are tested.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTwo control formats provided:\u003c\/strong\u003e Both E1A linear DNA Control (lyophilized) and E1A non-linear DNA Control are supplied so users can choose according to validation needs.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX supplied in ready-to-use formats to simplify setup; protect light-sensitive reagents from light.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, ABI 7500, CFX96); thermal and detector settings provided for instrument configuration.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1A DNA from host cells (e.g., HEK293, 293T) in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing and final product testing for residual host-cell E1A DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and QC workflows requiring absolute quantitation of E1A sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A linear DNA Control (lyophilized powder × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A non-linear DNA Control (50 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A Primer \u0026amp; Probe MIX (300 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow laboratory safety procedures; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration dates. Do not use if reagents show precipitate or cloudiness (heat DDB at 37 °C to clarify if necessary).\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, standards and IPC. Use triplicates for samples and standards for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific settings should be adapted to the user's instrument and software version; follow provided detector\/dye assignments and thermal profile as guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitation of residual E1A DNA from host cell-derived samples (for example, HEK293 and 293T) and is applicable to samples collected during biopharmaceutical manufacturing (in-process and final product). Samples must be purified prior to qPCR according to the recommended extraction workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is the standard curve prepared and what dynamic range is covered?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are prepared by serial 10-fold dilutions of the provided DNA controls. The linear DNA control stock is 4.97 × 10^8 copies\/μL (diluted down to 4.97 × 10^1 copies\/μL across ST1–ST6); the non-linear control stock is 2.98 × 10^7 copies\/μL (diluted down to 2.98 × 10^2 copies\/μL across ST0–ST5). At least five standard points (six for linear control) are recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What quality criteria should I use to accept a run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35. IPC Ct for sample should be within ±1.0 Ct of IPC Ct for NCS; significant shifts indicate inhibition. Verify standard curve slope, intercept and R^2 before reporting sample quantities.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the limit of quantification (LOQ) provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eA numeric LOQ is not specified in the kit documentation. Users should determine the proven LOQ during method validation for their specific matrix and instrument. If proven LOQ is below the lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173426094261,"sku":"1101109","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1A_DNA_Quantitation_Kit.png?v=1742280136"},{"product_id":"residual-e1b-dna-quantitation-kit-shentek®","title":"Residual E1B DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1B DNA Quantitation Kit is designed for absolute quantitation of residual E1B DNA derived from host cells (e.g., HEK293 and derivative cell lines such as 293T and 293F) in various stages of biopharmaceutical manufacturing (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (qPCR) assay with an internal positive control (IPC) to monitor PCR performance and includes an E1B linear DNA Control for preparation of standard curves and for detection of linearized samples. The kit is supplied for 100 reactions and is intended for research use only. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by fluorescent qPCR (FAM-labeled target, VIC-labeled IPC, ROX passive reference)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eST1 to ST6: 4.34 × 10^6 to 4.34 × 10^1 copies\/μL (prepared from E1B linear DNA Control; original ST = 4.34 × 10^8 copies\/μL before serial dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL, E1B Primer\u0026amp;Probe MIX 2.8 μL, IPC MIX 1.3 μL (total qPCR MIX = 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00; Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument settings \/ analysis\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 for both E1B-DNA and IPC; Automatic baseline; Standard curve (absolute quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control acceptance\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; a significantly higher Ct(IPC) indicates potential PCR inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input for preparation\u003c\/td\u003e\r\n \u003ctd\u003e100 μL test sample (or 100 μL DDB for negative control sample preparation prior to extraction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under stated conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification \/ limit of detection\u003c\/td\u003e\r\n \u003ctd\u003eNot numerically specified in the manual. Users should establish LOD\/LOQ by validation; standard curve lowest point in user protocol is 4.34 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh specificity and sensitivity:\u003c\/strong\u003e Fluorescent qPCR assay with sequence-specific primers and probe for E1B enabling specific detection of residual E1B DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Included IPC MIX monitors PCR performance and helps identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX minimize setup errors and speed assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eE1B linear DNA Control:\u003c\/strong\u003e Supplied lyophilized linear DNA control for preparation of serial dilutions and construction of an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on multiple real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, qTOWER3G, LightCycler480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFor residual host cell DNA testing:\u003c\/strong\u003e Designed specifically to quantitate residual E1B DNA from HEK293 and derivative cell lines in biopharmaceutical samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1B DNA in biopharmaceutical products (in-process and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in HEK293 and derivative cell lines (e.g., 293T, 293F)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host cell DNA analysis\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA clearance and downstream process effectiveness\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B linear DNA Control (lyophilized powder × 1 tube) — Part No. NNA014\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes) — Part No. NNB002\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B Primer \u0026amp; Probe MIX (300 μL × 1 tube) — Part No. NNC034\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube) — Part No. NNC066\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes) — Part No. NND001\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free consumables and low-retention tips to avoid contamination and loss.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer\u0026amp;Probe MIX, IPC MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup to reduce contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure lyophilized E1B linear DNA Control is fully dissolved (add 55 μL ddH2O and mix as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 or undetermined; check IPC Ct to assess inhibition (sample IPC Ct must be within ±1.0 Ct of NCS IPC Ct).\u003c\/p\u003e\r\n \u003cp\u003e• Establish assay-specific LOD\/LOQ during method validation; lowest manual standard = 4.34 × 10^1 copies\/μL.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses fluorescent quantitative PCR (qPCR) for absolute quantitation of E1B DNA (FAM-labeled target, VIC-labeled IPC, ROX passive reference).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit and what is the recommended reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. The recommended total reaction volume is 30 μL (20 μL qPCR MIX + 10 μL sample\/control).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the standard curve range included in the user protocol and is LOQ specified?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol prepares a standard curve with concentrations from 4.34 × 10^6 down to 4.34 × 10^1 copies\/μL (ST1–ST6). A numeric LOQ\/LOD is not specified in the manual; users should determine assay-specific LOD\/LOQ during validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIPC (internal positive control) monitors PCR performance and sample inhibition. The Ct(IPC) of a sample should be within ±1.0 Ct of the Ct(IPC) of the NCS. The NTC should have Ct ≥ 35 or be undetermined. A significantly higher Ct(IPC) in a sample indicates possible inhibition; consider dilution or purification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173461713077,"sku":"1101110","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1B_DNA_Quantitation_Kit.png?v=1742280232"},{"product_id":"residual-pg13-dna-quantitation-kit-shentek®","title":"Residual PG13 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual PG13 DNA Quantitation Kit is designed to quantify residual PG13 host cell DNA in biopharmaceutical samples at different stages (in-process through final product). The kit uses quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with sensitivity at the femtogram (fg) level. The kit includes a PG13 DNA Control as a reference standard and reagents to prepare standard curves and perform absolute quantitation. For extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide. The kit is supplied for research use only and provides reagents sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (qPCR) using FAM-labeled probe\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to the femtogram (fg) level (analytical sensitivity stated as fg-level in kit documentation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (pg\/μL)\u003c\/td\u003e\r\n \u003ctd\u003e3000 (ST0 stock) and working standards ST1=300, ST2=30, ST3=3, ST4=0.3, ST5=0.03, ST6=0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (demonstrated by standards)\u003c\/td\u003e\r\n \u003ctd\u003eApproximately 300 to 0.003 pg\/μL (prepared standard series provides \u0026gt;5 orders of magnitude coverage from working standards)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePer-reaction reagent volumes (qPCR MIX)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + PG13 Primer\u0026amp;Probe MIX 3 μL (total 20 μL qPCR MIX per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Roche LightCycler 480 II (and other compatible real-time PCR systems supporting FAM detection and absolute quantitation mode)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02 (PG13-DNA detector); Auto baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger than mean Ct of lowest standard (or \u0026lt; QL when QL \u0026lt; lowest standard); verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C storage; kit components stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram (fg) level for detection of trace residual PG13 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA Control and a serial dilution standard curve to perform absolute quantitation (standard curve method).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e qPCR Reaction Buffer and PG13 Primer \u0026amp; Probe mix provided for streamlined plate setup and consistent reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Detailed instructions for serial dilutions, controls (ERC, PCS, NCS, NTC), qPCR mix preparation and recommended analysis parameters.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eResearch use supply:\u003c\/strong\u003e Supplied for Research Use Only (RUO) and includes reagents sufficient for 100 reactions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical process intermediates\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual PG13 DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA assays targeting PG13 cell line\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control monitoring of host cell DNA removal\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer \u0026amp; Probe MIX (NNC101) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C for working use as directed)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards and controls carefully; include at least five concentrations in the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before running the qPCR.\u003c\/p\u003e\r\n \u003cp\u003e• Follow recommended analysis settings (Manual Ct, Threshold 0.02, Auto Baseline) or validate settings per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• QC acceptance: ERC recovery 50%–150%; NTC should be ≥35 cycles or undetermined; NCS Ct must be higher than the lowest standard Ct (or meet QL criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses quantitative PCR (qPCR) with a FAM-labeled probe for absolute quantitation using a standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sensitivity can I expect from the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit documentation reports sensitivity at the femtogram (fg) level. Working standard series are provided down to 0.003 pg\/μL (3 fg\/μL), but laboratories should validate the limit of quantitation (QL) in their own workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reagents be prepared and loaded?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare qPCR MIX (17 μL qPCR Reaction Buffer + 3 μL Primer\u0026amp;Probe MIX per reaction), aliquot 20 μL per well and add 10 μL of sample or standard (total 30 μL reaction). Include NTC, NCS, PCS and ERC controls and run standards in triplicate. Seal plate, mix and centrifuge briefly before starting the run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should be ≥35 cycles or undetermined. NCS Ct should be larger than the mean Ct of the lowest standard or meet QL criteria determined in validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173516992693,"sku":"1101123","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Quantitation_Kit.png?v=1742289744"},{"product_id":"residual-mrc-5-dna-quantitation-kit-shentek®","title":"Residual MRC-5 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MRC-5 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual MRC-5 host cell DNA in biopharmaceutical samples at in-process and final product stages. The kit enables rapid, specific, and reliable absolute quantitation down to the femtogram (fg) level using an included MRC-5 DNA Control as reference standard. The kit is supplied as reagents for 100 reactions (Product No.: 1101124) and is intended for research use only. For sample extraction guidance, refer to the corresponding SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MRC-5 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (FAM reporter)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ analytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level; lowest standard used 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eStandard points: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection value of NTC should be ≤ 6.00 fg\/µL (or laboratory-specific criteria after method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction mix per test\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: qPCR Reaction Buffer 17 µL + MRC-5 Primer \u0026amp; Probe MIX 3 µL (prepare 20 µL qPCR MIX then add 10 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95°C 10:00 (1 cycle); Denaturation: 95°C 0:15 (40 cycles); Annealing\/extension and read: 60°C 1:00 (40 cycles)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 example provided); compatible with standard real-time PCR instruments\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format and size\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions; Product No. 1101124; Version A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (individual components stored at -20 °C as labeled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eResult reporting units\u003c\/td\u003e\r\n \u003ctd\u003eMean Quantity reported in pg\/µL (results exportable from instrument software)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ acceptance\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard; NTC detection value ≤ 6.00 fg\/µL; ERC recovery 50–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to the femtogram level (lowest standard 0.03 pg\/µL = 30 fg\/µL) enabling detection of trace residual host-cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes MRC-5 DNA Control for construction of a standard curve and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe mix simplify setup and reduce preparation variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example settings provided for common real-time PCR platforms (ABI 7500, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Appropriate for in-process and final product testing, and for method validation and routine QC of residual host cell DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MRC-5 host-cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product QC testing for host-cell DNA content\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery assessment (using ERC samples)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 DNA Control (NNA062) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 Primer \u0026amp; Probe MIX (NNC118) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (remaining unused DDB store at 2–8 °C after thaw; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Read Material Safety Data Sheets (MSDS) before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use RNase\/DNase-free consumables and low-retention tips; follow sterile technique.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points; perform method validation to select sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix on microplate shaker and briefly centrifuge before running.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50–150%; NCS Ct should be larger than the mean Ct of the lowest standard; NTC signal ≤ 6.00 fg\/µL (or lab-defined criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to detect residual MRC-5 DNA at the femtogram (fg) level. The lowest standard provided is 0.03 pg\/µL (30 fg\/µL). The NTC acceptance criterion is ≤ 6.00 fg\/µL; final LOD should be established during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are supplied in a single kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supplies reagents for 100 reactions (Product No. 1101124).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What instrument settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings for ABI 7500 (SDS v1.4) are provided: 30 µL reaction volume; Activation 95°C 10:00; 40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C. If using other instruments, adapt thermal profile and settings according to the instrument documentation and validate accordingly.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store kit components and how long are they stable?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C as labeled. The kit components can be stored under appropriate conditions for up to 24 months. After thawing, store unused DNA Dilution Buffer (DDB) at 2–8 °C; if it becomes cloudy, incubate at 37 °C until clear.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175198253237,"sku":"1101124","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MRC-5_DNA_Quantitation_Kit.png?v=1742289936"},{"product_id":"residual-bovine-dna-quantitation-kit-shentek®","title":"Residual Bovine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 b0C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK4 Residual Bovine DNA Quantitation Kit is designed to quantitate residual bovine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The assay provides rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit includes bovine DNA control, primer \u0026amp; probe mix, internal positive control (IPC) mix, qPCR reaction buffer and DNA dilution buffer. Compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500). For sample extraction recommendations refer to the SHENTEK Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). For Research Use Only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1101112\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical principle\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (kit description states fg-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Calibration range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared standards from 3000 pg\/bcl down to 0.03 pg\/bcl (ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 bL total reaction volume (20 bL qPCR MIX + 10 bL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 bL, Bovine primer \u0026amp; probe MIX 2.8 bL, IPC MIX 1.3 bL (total 20 bL); add 10 bL sample or standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG (optional) 25 b0C 10:00; Activation 95 b0C 10:00; 40 cycles of Denaturation 95 b0C 00:15 and Annealing\/Extension 63 b0C 00:40 (fluorescence read during 63 b0C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria (IPC)\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within b10.0 Ct of NCS (if significantly higher, sample inhibition suspected)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction Recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery rate acceptance range: 50% e2 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NCS\/NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NCS should be larger than mean Ct of the lowest standard; NTC Ct should be at least 2 cycles larger than mean Ct of lowest standard (or follow lab-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 settings example provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 b0C, kit components can be stored at appropriate conditions for up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 bL purified test sample or 10 bL standards\/DDB per reaction (per recommended workflow)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram level (fg), enabling detection of trace residual bovine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a standard-curve based absolute quantitation method with serially diluted bovine DNA controls.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control:\u003c\/strong\u003e Includes an IPC mix to monitor PCR inhibition and validate sample results.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR MIX:\u003c\/strong\u003e Pre-formulated reaction buffer and primer\/probe mix minimize setup steps and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (example settings provided for ABI 7500 and SHENTEK-96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual bovine DNA in biomaterials and biological raw materials\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics, bioprocess intermediates and medical device materials\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and lot release testing for products where bovine-derived contaminants must be monitored\u003c\/li\u003e\r\n \u003cli\u003eResearch applications assessing bovine DNA contamination and extraction recovery\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine DNA Control (NNA032) 100 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine Primer \u0026amp; Probe MIX (NNC042) 300 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 bL d7 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL d7 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination (UV-decontaminate surfaces and equipment, 75% ethanol disinfection).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2-8 b0C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light; include appropriate controls (NTC, NCS, ERC) and run at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer becomes cloudy, warm at 37 b0C until clear; store unused DDB at 2-8 b0C.\u003c\/p\u003e\r\n \u003cp\u003e• UNG treatment step is optional and should be included only if UNG enzyme is added to the reaction mix.\u003c\/p\u003e\r\n \u003cp\u003e• Follow IPC and ERC acceptance criteria: IPC Ct within b11.0 of NCS; ERC recovery 50% to 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) or limit of quantitation (LOQ) for the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for fg-level sensitivity. The provided standard curve ranges from 3000 pg\/bcl to 0.03 pg\/bcl; validated LOD\/LOQ should be established by the user in their laboratory during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR instrument settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings are provided for ABI 7500 (SDS v1.4): optional UNG 25 b0C 10:00; activation 95 b0C 10:00; 40 cycles of 95 b0C 00:15 and 63 b0C 00:40 (fluorescence read at 63 b0C). Adjust settings as needed for other platforms and software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the supplied Bovine DNA Control serially in DNA Dilution Buffer to generate standards: ST0 = 30000 pg\/bcl (stock) then ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl. Include at least five concentrations in the curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included in each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), Extraction Reference Control (ERC), and serial standards (ST1-ST6). Run samples and controls in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175224959157,"sku":"1101112","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Bovine_DNA_Quantitation_Kit.png?v=1742290060"},{"product_id":"residual-procine-dna-quantitation-kit-shentek®","title":"Residual Procine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Porcine DNA Quantitation Kit is designed to quantify residual porcine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The kit provides a rapid, specific and reliable absolute quantitation assay with sensitivity down to the femtogram (fg) level. The product is supplied for 100 reactions (Product No. 1101113) and is intended for research use only. For sample extraction instructions, refer to the SHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). The kit includes internal positive control (IPC) reagents and a porcine DNA standard control to build a standard curve for absolute quantitation.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual porcine DNA in biomaterials (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (kit optimized for absolute quantitation down to 0.03 pg\/µL standard point)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared: ST0 30000 pg\/µL (stock), ST1 3000 pg\/µL, ST2 300 pg\/µL, ST3 30 pg\/µL, ST4 3 pg\/µL, ST5 0.3 pg\/µL, ST6 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 µL; Porcine Primer \u0026amp; Probe MIX 2.8 µL; IPC MIX 1.3 µL; (optional UNG enzyme 0.1 U)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eOptional UNG 25 °C 10:00; Activation 95 °C 10:00; 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 00:40 (annealing\/extension, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorophores \/ channels\u003c\/td\u003e\r\n \u003ctd\u003ePorcine-DNA: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for Porcine-DNA and 0.02 for IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of the NCS (negative control) IPC Ct; large shift indicates inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery criteria (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard concentration; NTC should be 2 cycles larger than mean Ct of lowest concentration (or laboratory-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months; key reagents stored at –20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System; other real-time PCR systems supported with appropriate setup\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Optimized qPCR assay capable of femtogram-level detection for low-abundance residual porcine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes porcine DNA control and recommended serial dilutions to generate an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IPC):\u003c\/strong\u003e IPC MIX included to monitor extraction and PCR inhibition for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated AT qPCR Reaction Buffer and Porcine Primer \u0026amp; Probe MIX reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarryover prevention option:\u003c\/strong\u003e Supports optional UNG treatment step when UNG enzyme is included to prevent carryover contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Detailed guidance for serial dilution, plate layout, thermal cycling and result analysis for reproducible assays.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual porcine DNA in biomaterials (e.g., tissue-derived products, implants, scaffolds)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for animal-derived material processing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and extraction recovery assessment using ERC samples\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of porcine DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine DNA Control (NNA031) 100 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 µL × 2\u003c\/span\u003e\u003cstrong\u003e–20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine Primer \u0026amp; Probe MIX (NNC043) 300 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation: User Guide (this manual)\u003c\/span\u003e\u003cstrong\u003eambient\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes by UV irradiation (30 minutes) and 75% ethanol prior to setup to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents at –20 °C; check expiration dates on labels. Protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations when preparing the standard curve; recommended serial dilution series provided (ST1–ST6).\u003c\/p\u003e\r\n \u003cp\u003e• Prepare NTC, NCS and ERC controls as instructed. Test samples and controls in triplicate for reliable results.\u003c\/p\u003e\r\n \u003cp\u003e• If UNG is used, include 25 °C 10:00 UNG step in the thermal program; UNG is optional but recommended to prevent carryover.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC Ct values to assess inhibition; acceptable IPC Ct shift is ±1.0 relative to NCS.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery rate is 50%–150%; if outside this range investigate extraction and inhibition.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively measure residual porcine DNA in biomaterials using qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 µL (20 µL qPCR MIX + 10 µL sample or standard). qPCR MIX per reaction: AT qPCR Reaction Buffer 15.9 µL, Porcine Primer \u0026amp; Probe MIX 2.8 µL, IPC MIX 1.3 µL. Optional UNG 0.1 U per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the provided Porcine DNA Control with DNA Dilution Buffer to produce a serial dilution series: ST0 30000 pg\/µL then ST1 3000, ST2 300, ST3 30, ST4 3, ST5 0.3, ST6 0.03 pg\/µL. Include at least five concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What criteria indicate assay inhibition or failure?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC mean Ct is \u0026gt; ±1.0 Ct from the NCS IPC Ct, the sample may be inhibitory. ERC recovery outside 50%–150% or abnormal NTC\/NCS Ct values also indicate possible issues; troubleshoot extraction, sample dilution, or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175394730165,"sku":"1101113","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dna-Residual_Procine_DNA_Quantitation_Kit.png?v=1742290235"},{"product_id":"residual-cho-dna-size-analysis-kit-2g-shentek®","title":"Residual CHO DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Size Analysis Kit (2G) is a fluorescent quantitative PCR (FAM) based kit for rapid and specific quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA fragments across multiple size ranges in biopharmaceutical samples (from in-process to final products). The assay amplifies four discrete fragment sizes (95 bp, 110 bp, 215 bp and 523 bp) to produce an accurate size distribution profile. The kit includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, CHO DNA control for standard curves and DNA dilution buffer (DDB). The assay sensitivity reaches femtogram (fg) level. Refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction procedures. Product No.: 1103170-2. Reagents sufficient for 4 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e95 bp, 110 bp, 215 bp, 523 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eTarget probes: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection limit reaches femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (examples)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSuggested linear range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 0.03 – 300 pg\/µL (based on provided standard curve points)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL + CHO Primer\u0026amp;Probe MIX (per fragment) 2.8 µL + IPC MIX 1.3 µL = 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 µL (standard\/ST1–ST5, DDB for NCS, or purified sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/Read: 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selection; Threshold = 0.02; Automatic baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (kit components support ~400 reactions distributed across four fragment assays)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-size profiling:\u003c\/strong\u003e Simultaneous measurement strategy (four separate assays) for size distribution of CHO residual DNA (95, 110, 215, 523 bp) to characterize fragmentation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit at femtogram (fg) level enables quantitation of very low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated CHO Primer \u0026amp; Probe MIXes and qPCR Reaction Buffer simplify setup and reduce pipetting variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC mix included (VIC-labeled) to monitor inhibition and reaction performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600 plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA control and DNA dilution buffer for standard curves plus protocol for plate layouts and analysis.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation and size-distribution analysis of residual CHO DNA in final drug substance\/product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eRoutine QC testing of downstream purification and final product release (research and development settings)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB002)\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light (850 μL × 8 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-95 (NNC087)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-110 (NNC088)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-215 (NNC089)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-523 (NNC090)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069)\u003c\/span\u003e\u003cstrong\u003e–20 °C (550 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (NNA001)\u003c\/span\u003e\u003cstrong\u003e–20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C recommended after thaw; (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 Reactions | Method: qPCR (Fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free environment; use DNase-free consumables and low-retention tips to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% alcohol before use.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at –20 °C and check expiration dates. Kit stable up to 24 months under recommended storage.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (95, 110, 215, 523 bp). Include at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitates, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use NTC (no template control), NCS (negative control sample) and ERC (extraction reference control) as directed to monitor contamination and extraction recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Mix qPCR reactions thoroughly, seal plate, spin briefly and avoid bubble formation prior to run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit sensitivity reaches the femtogram (fg) level. Standard curve points provided include concentrations down to 0.03 pg\/µL (30 fg\/µL) and a stock at 3000 pg\/µL; actual LOD may depend on instrument and sample matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which real-time PCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Linegene 9600 plus. Other instruments supporting FAM\/VIC detection and ROX (if needed) may be used — consult instrument documentation for detector\/dye settings.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at –20 °C, protect light-sensitive reagents from light. Under recommended storage the kit is stable for up to 24 months; always verify the expiration date on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How many reactions does the kit support and how are reactions prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 4 × 100 reactions. Typical reaction setup is 20 µL qPCR MIX + 10 µL sample = 30 µL total. Prepare separate qPCR mixes for each fragment assay (95\/110\/215\/523) as described in the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175424188597,"sku":"1103170-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Size_Analysis_Kit_2G.png?v=1742290344"},{"product_id":"residual-e-coli-dna-size-analysis-kit-2g-shentek®","title":"Residual E. coli DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E.coli DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) kit for rapid and specific quantitation of residual Escherichia coli (E.coli) host-cell DNA across different fragment sizes during biopharmaceutical development and manufacturing. The kit amplifies four target fragment lengths (85 bp, 103 bp, 220 bp and 550 bp) using FAM-labeled probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. It supports absolute quantitation via user-prepared standard curves prepared from the supplied E.coli DNA Control and DNA Dilution Buffer (DDB). The kit is supplied as ready-to-use primer\/probe mixes and reaction buffer for high-throughput testing and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). For extraction\/preparation of host-cell DNA prior to qPCR, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 103 bp, 220 bp, 550 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards provided)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standard curve points prepared from E.coli DNA Control: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eImplied sensitivity \/ lowest standard\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eLowest standard = 0.03 pg\/µL (30 fg\/µL); kit described as detecting at the femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR MIX per well: 20 µL; add 10 µL sample\/standard\/NTC\/NCS for a total reaction volume of 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Instrument reads fluorescence during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescent channels \/ detectors\u003c\/td\u003e\r\n \u003ctd\u003eTargets (E.coli fragments) - FAM; IPC - VIC; Passive reference - ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eUse Absolute Quantitation (Standard Curve). Analysis settings example: Manual Ct; Threshold = 0.02; Automatic baseline. Verify slope, intercept and R2 values.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of NCS Ct-IPC. Significant increase indicates possible inhibition.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC and NCS should be larger than mean Ct of lowest standard or show no significant amplification in FAM channel while showing normal VIC signal.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components: up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 and other real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment-size analysis (four assays):\u003c\/strong\u003e Designed to quantify four discrete E.coli DNA fragment lengths (85, 103, 220 and 550 bp) to determine size distribution of residual host DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection capability down to femtogram (fg) level; lowest provided standard 0.03 pg\/µL (30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Primer \u0026amp; Probe MIX for each fragment (85, 103, 220, 550) simplifies setup and reduces handling error.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC (VIC) included to monitor inhibition and sample integrity; IPC acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility and throughput:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms and formatted for 96-well plates; reagents sufficient for 4 × 100 reactions (high-throughput).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Supplied E.coli DNA Control and dilution scheme enables generation of standard curves for absolute quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E.coli host-cell DNA in biopharmaceutical process and final products\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during process development\u003c\/li\u003e\r\n \u003cli\u003eQuality control and characterization of DNA fragment size distribution in purified biologics\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA assessment (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli DNA Control (NNA002) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-85 (NNC110) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-103 (NNC111) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-220 (NNC112) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE.coli Primer \u0026amp; Probe MIX-550 (NNC113) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (after thaw, store at 2–8 °C; if cloudy heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 400 reactions (4 × 100) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate personal protective equipment: eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use DNase-free, low-retention consumables; irradiate work surfaces and pipettes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (four assays).\u003c\/p\u003e\r\n \u003cp\u003e• qPCR reaction setup: prepare qPCR MIX (20 µL) and add 10 µL sample\/standard for 30 µL total reaction volume.\u003c\/p\u003e\r\n \u003cp\u003e• Analysis guidance: use Manual Ct, Threshold = 0.02 (example); verify slope, intercept and R2 of standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: mean Ct-IPC for sample should be within ±1.0 Ct of NCS Ct-IPC; elevated IPC Ct suggests inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC\/NCS should show no significant amplification in FAM channel or Ct greater than lowest standard; VIC signal should be present for IPC.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103171-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use to quantify residual E.coli host-cell DNA and determine fragment size distribution in biopharmaceutical samples (in-process and final products). It is RUO and not for clinical diagnostic use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instrument channels should I use for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse FAM channel(s) for E.coli target fragment detection, VIC for the internal positive control (IPC), and ROX as the passive reference according to your instrument configuration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the lowest concentration the kit can detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). The kit description indicates detection at the femtogram level; actual limit of detection should be confirmed during in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standards and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw E.coli DNA Control and DDB at 2–8 °C or on ice. Dilute DNA Control to ST0 = 3000 pg\/µL then prepare serial dilutions to produce ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentrations, 1 NTC and 1 NCS per run; run samples in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175438213301,"sku":"1103171-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Size_Analysis_Kit_2G.png?v=1742290413"},{"product_id":"residual-mdck-dna-size-analysis-kit-shentek®","title":"Residual MDCK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Size Analysis Kit is designed to quantify fragment sizes of residual MDCK host cell DNA in biopharmaceutical samples across process stages (in‑process to final products). The kit utilizes a real‑time PCR (qPCR) approach to rapidly and specifically quantify MDCK DNA fragments using FAM‑labeled probes. It amplifies four discrete fragment lengths (84 bp, 142 bp, 204 bp and 504 bp) to determine size distribution with a limit of detection in the femtogram (fg) range. An internal positive control (IPC, VIC) is included to monitor inhibition and assay performance. For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). Reagents supplied support 4 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e84 bp, 142 bp, 204 bp, 504 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003efemtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume and template input\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; MDCK Primer \u0026amp; Probe MIX 2.8 μL (per target); IPC MIX 1.3 μL; total 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions (example \/ ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30 (read fluorescence); 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC. If significantly higher, sample may be inhibitory. NTC Ct should be ≥ 35 or undetermined.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions total)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFour discrete fragment assays:\u003c\/strong\u003e Simultaneous\/parallel assays for 84 bp, 142 bp, 204 bp and 504 bp fragments for size distribution profiling.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Limit of detection at the femtogram level for detection of low‑abundance residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR based, rapid and specific:\u003c\/strong\u003e Real‑time PCR with FAM probe detection provides rapid and specific quantitation of MDCK residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal inhibition control (IPC):\u003c\/strong\u003e VIC‑labeled IPC included to monitor sample inhibition and assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use primer\/probe mixes:\u003c\/strong\u003e Preformulated MDCK Primer \u0026amp; Probe MIXes for each fragment length reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for SHENTEK‑96S, ABI 7500, LightCycler 480 II and other real‑time PCR systems.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution for process development and validation\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for residual host cell DNA in vaccine and biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and sample inhibition assessment using IPC\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑84 (NNC025) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑142 (NNC026) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑204 (NNC027) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX‑504 (NNC028) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C (refer to label)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (not for diagnostic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (84, 142, 204, 504 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five concentrations for the standard curve and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct‑IPC of sample should be within ±1.0 of NCS Ct‑IPC; if higher, sample may be inhibitory — consider sample recovery testing.\u003c\/p\u003e\r\n \u003cp\u003e• NTC criterion: Ct for NTC should be ≥ 35 cycles or undetermined.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and spin samples before running.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components as labeled and check expiration dates before use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103175.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103175.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit contains primer\/probe mixes for four fragment sizes: 84 bp, 142 bp, 204 bp and 504 bp.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL qPCR MIX + 10 μL template\/sample). Prepare qPCR MIX according to the protocol tables for each fragment assay.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How sensitive is the assay and what standards should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay has a limit of detection at the femtogram level. Prepare the supplied MDCK DNA Control serial dilutions (examples: ST0 3000 pg\/μL down to ST6 0.003 pg\/μL) and include at least five standard concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibitory?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eMonitor the IPC (VIC) signal. The mean Ct‑IPC for a sample should be within ±1.0 of the NCS Ct‑IPC. A significantly higher Ct‑IPC indicates potential inhibition and you should evaluate sample recovery or dilution.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175564304565,"sku":"1103175","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Size_Analysis_Kit.png?v=1742291658"},{"product_id":"residual-sf9-residual-dna-size-analysis-kit-shentek®","title":"Residual Sf9 Residual DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9 DNA Size Analysis Kit is a qPCR-based kit for rapid, specific quantitation and size distribution analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (from in‑process to final products). The assay amplifies four target fragment lengths (87 bp, 118 bp, 225 bp and 500 bp) using FAM-labeled detection and includes an internal positive control (IPC, VIC). The kit achieves sensitivity at the level of femtograms (fg). This package contains reagents to perform 4 × 100 reactions (primer\/probe mixes for four fragment sizes, qPCR reaction buffer, Sf9 DNA control, IPC mix and DNA dilution buffer). For DNA extraction\/sample preparation refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e87 bp, 118 bp, 225 bp, 500 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity at femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (recommended)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, ST6 = 0.003 pg\/µL (Note: MIX-87 and MIX-118: ST1–ST6; MIX-225 and MIX-500: ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per well = 20 µL; template\/sample = 10 µL; total reaction volume = 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9 Primer\u0026amp;Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling profile\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles (instrument reads fluorescence during extension step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt and analysis thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold = 0.02 (FAM); VIC (IPC) threshold = 0.1; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification; sample Ct-IPC should be within ±1.0 Ct of NCS Ct-IPC (IPC used to detect inhibition)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eRecommend triplicates for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility \/ instruments\u003c\/td\u003e\r\n \u003ctd\u003eValidated on SHENTEK-96S, ABI 7500, and LightCycler 480 II; compatible with other real-time PCR systems (follow instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable for up to 24 months when stored as indicated on labels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative size analysis of residual Sf9 host-cell DNA in biopharmaceutical samples (research applications)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFour-size multiplexed analysis (separate assays):\u003c\/strong\u003e Designed to determine size distribution by amplifying four discrete fragment lengths (87, 118, 225, 500 bp) for accurate fragment-size profiling.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e LOD at the femtogram level enables detection and quantitation of trace residual Sf9 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Standard-curve (absolute quantitation) workflow with serial dilutions and recommended concentration points for accurate quantification.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC (VIC channel) included to monitor reaction integrity and identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mixes:\u003c\/strong\u003e Pre-formulated Sf9 Primer \u0026amp; Probe MIXes for each fragment length reduce setup variability and save time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, LightCycler 480 II); protocol includes instrument-specific guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eResidual DNA analysis in final biologic drug products derived from Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eSize distribution profiling of host-cell DNA fragments (87–500 bp range)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC for host DNA clearance processes (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 DNA Control (NNA033), 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB002), 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-87 (NNC060), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-118 (NNC061), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-225 (NNC062), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9 Primer\u0026amp;Probe MIX-500 (NNC063), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label (-20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB store at 2–8 °C after thawing)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surface, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at temperatures indicated on labels; kit stable up to 24 months.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear; store unused DDB at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length; include at least five standard points.\u003c\/p\u003e\r\n \u003cp\u003e• Run technical replicates (triplicates recommended) and include NTC and NCS controls.\u003c\/p\u003e\r\n \u003cp\u003e• Interpret IPC Ct: sample mean Ct-IPC should be within ±1.0 of NCS Ct-IPC; higher IPC Ct indicates inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103177.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103177.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume and reaction setup should I use for each well?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 20 µL qPCR MIX plus 10 µL of template (purified sample, NCS or standard) for a total reaction volume of 30 µL per well.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which standard concentrations are recommended for the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse the recommended standard series: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL, and ST6 = 0.003 pg\/µL. Note: use ST1–ST6 for MIX-87 and MIX-118; use ST1–ST5 for MIX-225 and MIX-500.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I know if my sample contains PCR inhibitors?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (VIC) Ct value: the sample mean Ct-IPC should be within ±1.0 Ct of the NCS Ct-IPC. A significantly higher IPC Ct in the sample indicates possible inhibition; consider dilution or additional purification and retest.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15; Annealing 60 °C for 00:30; Extension 72 °C for 01:30; 40 cycles. Instrument reads fluorescence during the extension step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175577018549,"sku":"1103177","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_Residual_DNA_Size_Analysis_Kit.png?v=1742292573"},{"product_id":"residual-human-dna-size-analysis-kit-2g-shentek®","title":"Residual Human DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Human DNA Size Analysis Kit (2G) is designed for the rapid and specific quantitation of residual human DNA fragments of different sizes in biopharmaceutical samples (from in‑process to final products) using real‑time PCR (qPCR). The assay amplifies four distinct fragment lengths (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer \u0026amp; probe mixes for each fragment, an internal positive control (IPC), qPCR Reaction Buffer, DNA dilution buffer and a Human DNA Control for standard curve generation. For DNA extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). This product is intended for Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFragment lengths amplified\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; standard curve down to 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (mix + template)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR mix per reaction = 20 µL (15.9 µL qPCR Reaction Buffer + 2.8 µL Primer\u0026amp;Probe Mix + 1.3 µL IPC MIX) + 10 µL template = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold guidance\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-75 = 0.04; Human-122\/244\/562 = 0.02. IPC Ct of sample should be within ±1.0 of NCS.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (400 reactions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable up to 24 months (check label expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A and other real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex fragment analysis:\u003c\/strong\u003e Assays four discrete human DNA fragment sizes (75, 122, 244 and 562 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram range with standard curve points to 0.03 pg\/µL.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment and IPC MIX to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC-focused workflow:\u003c\/strong\u003e Includes Human DNA Control for standard curves, NTC and NCS recommendations, and IPC monitoring for inhibition\/recovery.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms and configurable for different software\/instruments.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human DNA in biopharmaceutical products (in-process to final product)\u003c\/li\u003e\r\n \u003cli\u003eHost cell \/ residual DNA monitoring for process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing workflows for biologics\u003c\/li\u003e\r\n \u003cli\u003eComparative fragment size distribution analysis of residual DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-75 (NNC008) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-122 (NNC009) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-244 (NNC010) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer\u0026amp;Probe MIX-562 (NNC011) 300 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 µL ×1\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL ×3\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions (400 reactions) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points, NTC and NCS; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC Ct of sample should be within ±1.0 of NCS; significantly higher IPC Ct suggests assay inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC and NCS Ct values should be larger than mean Ct of the lowest standard concentration in the FAM channel.\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters and thresholds according to instrument model and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103173.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103173.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How should I store the kit and reagents?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore primer\/probe mixes, IPC MIX, Human DNA Control and qPCR Reaction Buffer at -20 °C. Store DNA Dilution Buffer (DDB) at 2–8 °C. The kit components are stable up to 24 months; verify expiration dates on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve should I use for quantitation?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 5‑point standard curve for each fragment using the provided Human DNA Control serial dilutions: 300, 30, 3, 0.3 and 0.03 pg\/µL (ST1–ST5). Include NTC and NCS controls and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions and reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR mix + 10 µL template). Thermal cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30 with fluorescence read during extension.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the IPC Ct indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf IPC Ct for a sample is significantly higher than the NCS (outside ±1.0), suspect inhibition. Consider re‑extracting or diluting the sample, check sample recovery (if ERC included), and reassess prior to reporting.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175607951541,"sku":"1103173","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Size_Analysis_Kit_2G.jpg?v=1742292680"},{"product_id":"residual-bhk-dna-size-analysis-kit-shentek®","title":"Residual BHK DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Size Analysis Kit is designed for quantitative measurement of residual BHK host-cell DNA fragments across different fragment sizes in biopharmaceutical samples (from in-process samples to final products). The assay uses quantitative PCR with FAM-labeled probes to amplify four discrete fragment sizes (81 bp, 134 bp, 216 bp and 589 bp) to determine size distribution and quantity. The kit provides high sensitivity with a reported limit of detection (LOD) at the femtogram (fg) level. An internal positive control (IPC, VIC channel) is included to monitor inhibition and extraction\/reaction performance. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e81 bp, 134 bp, 216 bp, 589 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFemtogram (fg) level sensitivity (LOD stated as fg level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit instructions)\u003c\/td\u003e\r\n \u003ctd\u003eStandards used (ST1-ST5): 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (serial dilutions prepared from ST0 source)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR reaction volume: 30 µL total (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; BHK Primer \u0026amp; Probe MIX (per fragment) 2.8 µL; IPC MIX 1.3 µL; total 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30; 40 cycles. Fluorescence is read during the extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd\u003eTarget probes: FAM reporter for each BHK fragment assay; IPC: VIC reporter. ROX recommended as passive reference (ABI instruments).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles; NCS Ct should be greater than mean Ct of the lowest standard and show normal VIC amplification. IPC Ct of sample should be within ±1.0 of NCS IPC Ct; deviations indicate inhibition.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSample recovery rate (ERC-based) should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (stated in product label \/ packaging)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantitation (LOQ) not numerically specified in the user guide; users should refer to assay validation for LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti‑size quantitation:\u003c\/strong\u003e Simultaneous assessment of residual BHK DNA across four discrete fragment sizes (81, 134, 216, 589 bp) to provide size distribution information.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay sensitivity reaches femtogram (fg) level LOD for detection of low-abundance residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based specificity:\u003c\/strong\u003e TaqMan-style primer\/probe assays (FAM) deliver rapid and specific quantitation; IPC (VIC) monitors inhibition and reaction integrity.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe MIXes simplify assay setup and reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (examples: SHENTEK-96S, ABI 7500, Roche LightCycler 480) and configurable for other real-time PCR systems.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eSize-distribution analysis of residual DNA in in-process and final drug product samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing during upstream\/downstream processing and release testing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-81 (NNC072) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-134 (NNC073) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-216 (NNC074) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX-589 (NNC075) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 total reactions, as stated on product label) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (see compliance). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Avoid nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C (on ice), vortex and briefly centrifuge before use; protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curves (minimum five concentrations recommended) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR reaction setup: 20 µL qPCR MIX + 10 µL sample\/standard = 30 µL total per well; seal plates and centrifuge briefly prior to run.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35; IPC Ct of sample should be within ±1.0 of NCS IPC Ct; recovery rates should be between 50% and 150%.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear and store remaining DDB at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Limit of quantitation (LOQ) must be established\/validated by user; if LOQ is lower than lowest standard, NCS Ct should be below LOQ concentration.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103179.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103179.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What fragment sizes does this kit detect and quantify?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit amplifies and quantifies four discrete BHK host‑cell DNA fragment sizes: 81 bp, 134 bp, 216 bp and 589 bp.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay sensitivity (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports a limit of detection (LOD) at the femtogram (fg) level. Exact LOD\/LOQ values should be confirmed during in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What reaction volume and thermal cycling program should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 00:15, 60 °C 00:30, 72 °C 01:30. Fluorescence is read during the extension step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC, NTC and NCS results?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles. IPC Ct of a sample should be within ±1.0 of the NCS IPC Ct; a higher IPC Ct indicates potential inhibition. NCS Ct should be greater than the mean Ct of the lowest standard and exhibit normal VIC amplification. Recovery rate (ERC-based) should be 50–150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175635017909,"sku":"1103179","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Size_Analysis_Kit.jpg?v=1742292816"},{"product_id":"residual-pg13-dna-size-analysis-kit-shentek®","title":"Residual PG13 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual PG13 DNA Size Analysis Kit is designed to quantify residual PG13 host-cell DNA across different fragment sizes during biopharmaceutical production (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (FAM) assay to rapidly and specifically measure PG13 DNA fragments. The assay amplifies three discrete target fragment sizes (118 bp, 212 bp and 502 bp) to determine size distribution. The kit includes an internal positive control (IPC) and a PG13 DNA control for generation of absolute-quantitation standard curves. Limit of detection (LOD) reaches the femtogram (fg) level. For DNA extraction recommendations, refer to the companion SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e118 bp, 212 bp, 502 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eLOD reaches femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per 1 reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; PG13 Primer\u0026amp;Probe MIX (per target) 2.8 µL; IPC MIX 1.3 µL; total 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension: 72 °C 01:30 (read fluorescence) — 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument\/readout channels\u003c\/td\u003e\r\n \u003ctd\u003eFAM for PG13 targets; VIC for IPC; ROX used as passive reference (ABI7500 example)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) for sample should be within ±1.0 of NCS Ct(IPC); a significantly higher Ct indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery rate (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExpected ERC recovery between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC and NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥35 cycles or undetermined; NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n \u003ctd\u003eComponents stored at –20 °C; stable for up to 24 months (check label expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reagent format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 3 × 100 reactions (stated on product cover)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Three separate primer\/probe assays (118 bp, 212 bp, 502 bp) enable determination of residual DNA size distribution, not just total DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay LOD reaches femtogram (fg) levels for detection of trace residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA control and recommended serial dilution scheme to build standard curves for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition monitoring:\u003c\/strong\u003e IPC (VIC) included to monitor PCR inhibition and assay performance on a per-sample basis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer\u0026amp;Probe mixes reduce setup time and variability.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA fragmentation during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product quality control and release testing for residual host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for gene therapy\/viral vector production using PG13-derived material\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 µL × 6 tubes (protect from light)\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-118 (NNC083) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-212 (NNC084) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer\u0026amp;Probe MIX-502 (NNC085) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC070) — 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 3 × 100 reactions (300 reactions) | Method: qPCR (fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Work in a clean, DNase-free environment; decontaminate surfaces and equipment (UV 30 minutes and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and brief-spin before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store components at –20 °C; check expiration dates on labels.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitate, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: at least 5 standard points, NTC, and NCS; recommended technical triplicates for samples.\u003c\/p\u003e\r\n \u003cp\u003e• IPC performance: sample mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant deviation may indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction recovery control) recovery should be 50%–150%; investigate out-of-range recoveries.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥35 cycles or undetermined; NCS should be greater than lowest standard Ct and show VIC amplification.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103178.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103178.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms, including SHENTEK-96S, ABI 7500 series, and LightCycler 480. If using a different instrument, follow the instrument\/software documentation for detector and plate setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw PG13 DNA Control and DDB at 2–8 °C. Dilute the DNA Control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five standards in the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are acceptable IPC and recovery criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct(IPC) for a sample should be within ±1.0 of the NCS Ct(IPC). ERC recovery rates should fall between 50% and 150%; values outside this range warrant investigation for extraction efficiency or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if my DNA Dilution Buffer is cloudy?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf DDB is cloudy or contains precipitate, heat the buffer at 37 °C until it becomes clear, then cool and use. Store DDB at 2–8 °C after opening or per kit instructions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"3×100 Reactions","offer_id":45175694983349,"sku":"1103178","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Size_Analysis_Kit.jpg?v=1742292887"},{"product_id":"residual-hpv18-e6-e7-dna-size-analysis-kit-shentek®","title":"Residual HPV18 E6\/E7 DNA Size Analysis Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eApproximately 1 hour\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HPV18 E6\/E7 DNA Size Analysis Kit is a real-time PCR (qPCR) kit designed for rapid and specific quantitation of residual HPV18 E6 and E7 DNA fragments of different sizes (from HeLa cell origin) across biopharmaceutical sample types (in-process to final products). The kit amplifies four fragment lengths (E6: 100 bp and 288 bp; E7: 110 bp and 240 bp) using FAM-labeled target probes and includes an internal positive control (IPC, VIC) for inhibition monitoring. The kit includes HPV E6\/E7 DNA Control for standard curve generation and is intended for research use only. For sample extraction instructions refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget genes \/ fragments\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eHPV18 E6 (100 bp, 288 bp) and E7 (110 bp, 240 bp)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ channels\u003c\/td\u003e\r\n \u003ctd\u003eFAM-labeled probes for target fragments; VIC-labeled probe for IPC; ROX as passive reference (recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample or standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + primer\u0026amp;probe mix 3 μL (per target assay); IPC qPCR mix: qPCR Reaction Buffer 15.9 μL, IPC MIX 1.3 μL, ddH2O 2.8 μL (per 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during annealing\/extension)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ dynamic range (provided example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard curve points shown: 1×10^8 to 1×10^2 copies\/μL (ST0 = 1×10^8, ST1 = 1×10^7 ... ST6 = 1×10^2); at least five concentrations recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicates recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls included \/ required\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eHPV E6\/E7 DNA Control (quantified) for standard curve; NTC (no template control); NCS (negative control sample); IPC for inhibition monitoring\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInput\/sample volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample or 10 μL standard per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 4 × 100 reactions (as labeled on product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eUp to 24 months when stored at –20 °C (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNot specified in the user guide; recommended to determine during in-house validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInhibition acceptance criterion\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMean Ct of IPC for sample should be within ±1.0 of the NCS Ct-IPC; significant increase suggests inhibition and sample recovery testing is recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus and other real-time PCR systems (user must configure detectors\/filters accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFragment-size specific quantitation:\u003c\/strong\u003e Designed to amplify and quantify four distinct HPV18 E6\/E7 fragment sizes (E6:100 bp \u0026amp; 288 bp; E7:110 bp \u0026amp; 240 bp) to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReal-time qPCR with internal control:\u003c\/strong\u003e Uses FAM-labeled probes for targets and VIC-labeled IPC to monitor inhibition and assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated primer \u0026amp; probe mixes:\u003c\/strong\u003e Supplied primer \u0026amp; probe mixes for each fragment simplify setup and reduce pipetting errors; probes are light-sensitive (protect from light).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use standards:\u003c\/strong\u003e Includes quantified HPV E6\/E7 DNA Control and detailed serial dilution protocol for building standard curves and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Provides recommended plate layouts, qPCR conditions, and data analysis settings for ABI7500 (can be adapted to other instruments).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HPV18 E6\/E7 DNA (HeLa origin) in biopharmaceutical production\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and final product quality control for residual host cell DNA (HPV18-specific)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual DNA size-distribution analysis\u003c\/li\u003e\r\n \u003cli\u003eSample inhibition assessment and recovery evaluation using IPC\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX\u003c\/span\u003e\u003cstrong\u003e150 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE6-100 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE6-288 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE7-110 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE7-240 primer \u0026amp; probe MIX\u003c\/span\u003e\u003cstrong\u003e300 μL ×1 tube; –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e850 μL × multiple tubes (as supplied); –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB, NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL ×2 tubes; store at 2–8 °C (if cloudy, heat to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHPV E6\/E7 DNA Control (NNA030)\u003c\/span\u003e\u003cstrong\u003e50 μL ×1 tube; –20 °C (check label for concentration before dilution)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR (real-time PCR, absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mixes and IPC MIX from light; store kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes (UV 30 minutes recommended) and use dedicated pre- and post-PCR areas to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run NTC, NCS and IPC controls as instructed. NCS must be processed the same as test samples.\u003c\/p\u003e\r\n \u003cp\u003e• If IPC Ct for a sample is \u0026gt; ±1.0 compared to NCS IPC Ct, the sample may contain inhibitors; perform recovery testing or further sample cleanup.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific software settings and thresholds; example settings provided for ABI 7500 (Manual Ct, threshold 0.02, automatic baseline).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030307S-P.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the expected assay runtime?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe qPCR program provided runs activation 95 °C for 10 min plus 40 cycles of 95 °C for 15 s and 60 °C for 60 s, resulting in an approximate total runtime of ~1 hour per run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the supplied HPV E6\/E7 DNA Control, dilute to prepare ST0 at 1×10^8 copies\/μL, then perform serial 1:10 dilutions to generate ST1–ST6 (1×10^7 to 1×10^2 copies\/μL). Include at least five concentrations across the dynamic range and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if the IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample mean Ct-IPC is significantly higher than NCS Ct-IPC (\u0026gt;±1.0), the sample may be inhibitory. Perform recovery testing (ERC) in the same assay, consider additional sample cleanup or dilution, and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What is the assay sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eLOD\/LOQ are not specified in the user guide. Determine LOD\/LOQ, precision and accuracy during in-house validation using the provided standard curve and appropriate validation procedures.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). Products are intended for research use only.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175697965237,"sku":"SK030307S-P","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HPV18_E6_E7_DNA_Size_Analysis_Kit.jpg?v=1742292960"},{"product_id":"residual-293t-rna-quantitation-kit-shentek®","title":"Residual 293T RNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual 293T RNA Quantitation Kit is a one-step reverse transcription and fluorescence quantitative PCR (one-step RT-qPCR) assay designed to quantitate residual total RNA from 293T host cells in biopharmaceutical products at various manufacturing stages (in-process samples to final products). The kit uses specific primers and probes for 293T RNA and contains an Internal Positive Control (IPC) to monitor reaction performance and inhibition. The assay achieves femtogram-level sensitivity and is intended for research use. For nucleic acid extraction guidance, refer to SHENTEK® Residual Host Cell RNA Sample Preparation Kit (Product No. 1201205).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (claimed)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects residual 293T total RNA at femtogram (fg) level; lowest standard in recommended curve = 0.002 pg\/µL (2 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended standard curve concentrations: 200 pg\/µL (ST0) \/ Example curve used in protocol: ST1=20 pg\/µL, ST2=2 pg\/µL, ST3=0.2 pg\/µL, ST4=0.02 pg\/µL, ST5=0.002 pg\/µL — effective linear range demonstrated from 2000 pg\/µL (starting control dilution) down to 0.002 pg\/µL depending on preparation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 µL total reaction volume (15 µL qRT-PCR mix + 5 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e5 µL purified sample (or 5 µL standard\/NTC\/NCS) per 20 µL reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription: 50 °C for 15:00 (1 cycle); Activation: 95 °C for 0:30 (1 cycle); 45 cycles of Denaturation 95 °C 0:10 and Annealing\/Extension 60 °C 0:40 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dyes \/ channels\u003c\/td\u003e\r\n \u003ctd\u003e293T-RNA: FAM reporter; RNA-IPC: VIC reporter; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt-IPC of sample and NCS should be within ±1.0 Ct; significantly higher Ct-IPC in sample indicates possible inhibition. If IPC of NCS or sample fails, evaluate inhibition and recovery.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be 2 cycles greater than mean Ct of the lowest standard; Ct of NCS should be larger than mean Ct of lowest standard and show VIC signal amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePlate layout \/ replicates\u003c\/td\u003e\r\n \u003ctd\u003eRecommended: at least five standard concentrations, NTC, NCS, IPC-NCS and IPC for each test sample; triplicate wells per sample\/standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stable up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus and other real-time PCR systems that support FAM\/VIC\/ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eUnits of report\u003c\/td\u003e\r\n \u003ctd\u003eResults reported as mean quantity in pg\/µL (per instrument result export)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOne-step RT-qPCR:\u003c\/strong\u003e Reverse transcription and qPCR in a single-step workflow to reduce handling and contamination risk.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram level (standards include down to 0.002 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC primer\/probe mix included to monitor reaction performance and detect sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-mixed primers \u0026amp; probes:\u003c\/strong\u003e 293T-specific primer \u0026amp; probe mix and RNA IPC primer \u0026amp; probe mix provided for convenience and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated with multiple real-time PCR instruments (SHENTEK-96S, ABI 7500, CFX96, Linegene 9600plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for standard curve quantitation:\u003c\/strong\u003e Includes 293T RNA Control and recommended serial dilution scheme for absolute quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual 293T host cell total RNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eTesting of in-process and final product samples for host cell RNA residues\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and sample recovery studies for residual host cell RNA\u003c\/li\u003e\r\n \u003cli\u003eQuality control during downstream processing and release testing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e293T RNA Control (NNA052) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e293T RNA Primer \u0026amp; Probe MIX (NNC096) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNA IPC Primer \u0026amp; Probe MIX (NNC053) 200 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: One-step RT-qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent RNase contamination: use DNase\/RNase-free consumables, UV decontaminate work surfaces and tubes as described (30 min), disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (One Step qPCR Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Treat samples with DNase prior to detection to remove genomic DNA influence; optimize DNase digestion conditions for sample type.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five concentrations in the standard curve; run triplicates for standards and samples where possible.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: Ct-IPC for sample and NCS should be within ±1.0 Ct; significantly higher Ct-IPC indicates inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC and NCS acceptance criteria: NTC Ct should be ≥2 cycles above mean Ct of lowest standard; NCS Ct should be larger than mean Ct of lowest standard and show VIC signal.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201202.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201202.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents sufficient for 100 reactions (as stated on the product packaging).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 20 µL total reaction volume (15 µL qRT-PCR mix + 5 µL sample or control). Prepare qRT-PCR mix according to the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the thermal cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eReverse transcription 50 °C for 15:00, Activation 95 °C for 0:30, then 45 cycles of 95 °C 0:10 (denaturation) and 60 °C 0:40 (anneal\/extend; read fluorescence at 60 °C).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than IPC-NCS (greater than ±1.0 Ct difference), investigate inhibitors in the sample, consider sample dilution, re-extraction, or additional purification; prioritize recovery results if spike-in controls were run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181252337845,"sku":"1201202","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_293T_RNA_Quantitation_Kit.jpg?v=1742293531"},{"product_id":"car-tcr-transgene-copy-number-quantitation-kit","title":"CAR\/TCR Transgene Copy Number Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px 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#f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eSHENTEK® CAR\/TCR Transgene Copy Number Quantitation Kit is designed to quantify the copy number of target genes in human cell products derived from common HIV-1 lentiviral vectors (e.g., CAR or TCR genes integrated in CAR-T and TCR-T cell genomes). The kit uses a multiplex qPCR assay to detect DNA sequences related to the integration\/expression elements on the transfer plasmid and a Single Copy Gene (SCG) in human cells, enabling calculation of average copies of target gene per cell. The kit includes a company-produced reference standard and an internal positive control (IPC). The assay is intended for rapid, specific, reliable and universal lentiviral vector applications. For Research Use Only.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eProduct number\u003c\/td\u003e\n \u003ctd\u003eSK030221CA100\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReference standard initial concentration\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR\/TCR Control initial copy number: 2.33 × 10^8 copies\/μL\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStandard curve serial dilutions (copies\/μL)\u003c\/td\u003e\n \u003ctd\u003eST1: 2.33 × 10^6; ST2: 2.33 × 10^5; ST3: 2.33 × 10^4; ST4: 2.33 × 10^3; ST5: 2.33 × 10^2\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction volume\u003c\/td\u003e\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL template)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; CAR\/TCR Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReporter dyes \/ targets\u003c\/td\u003e\n \u003ctd\u003eCAR\/TCR target: FAM; SCG: CY5; IPC: VIC; ROX as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\n \u003ctd\u003eManual Ct threshold 0.02; Automatic baseline; verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria for IPC\u003c\/td\u003e\n \u003ctd\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; significant increase indicates inhibition\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eRecommended DNA input per well\u003c\/td\u003e\n \u003ctd\u003eRecommended 5 ng to 30 ng total DNA per well (adjust based on preliminary experiments)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCalculation formula\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eCAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample types\u003c\/td\u003e\n \u003ctd\u003ePurified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eMultiplex absolute quantitation:\u003c\/strong\u003e Simultaneous detection of CAR\/TCR target, single copy gene (SCG) and IPC in the same reaction for absolute copy number determination using a standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReference standard included:\u003c\/strong\u003e Proprietary reference standard supplied and pre-dilution scheme provided for a 5-point standard curve.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated CAR\/TCR Primer \u0026amp; Probe MIX and IPC MIX simplify assay setup and reduce pipetting error.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR platforms:\u003c\/strong\u003e Validated for use on SHENTEK-96S, ABI 7500, LineGene 9600plus, CFX96 and similar real-time PCR instruments.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eDesigned for lentiviral vector applications:\u003c\/strong\u003e Optimized for quantifying transgene copy numbers from HIV-1 derived lentiviral vectors (CAR\/TCR).\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQuantification of CAR or TCR transgene copy number in CAR-T and TCR-T cell products\u003c\/li\u003e\n \u003cli\u003eLentiviral vector integration assessment\u003c\/li\u003e\n \u003cli\u003ePreclinical and research cell therapy product characterization\u003c\/li\u003e\n \u003cli\u003eQuality control and batch testing for gene-modified cell products (research use)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Primer \u0026amp; Probe MIX (NNC032) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eIPC MIX (NNC066) 150 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C (store remaining DDB at 2–8 °C); -20 °C for long term per kit table\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eCAR\/TCR Control (NNA022) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex absolute quantitation, standard curve)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean environment: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol to avoid contamination.\u003c\/p\u003e\n \u003cp\u003e• Avoid assay inhibitors; monitor IPC Ct — if sample IPC Ct is significantly higher than NCS IPC Ct, sample may be inhibitory.\u003c\/p\u003e\n \u003cp\u003e• NTC and NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/p\u003e\n \u003cp\u003e• Prepare and run triplicates for standards, NTC, NCS and test samples where possible.\u003c\/p\u003e\n \u003cp\u003e• Recommended sample input per well: 5 ng–30 ng total DNA (optimize as needed).\u003c\/p\u003e\n \u003cp\u003e• Store kit components as labeled (-20 °C) and protect from light; kit stable up to 24 months. If DDB is cloudy, heat to 37 °C until clear.\u003c\/p\u003e\n \u003cp\u003e• Seal plates\/tubes, mix and briefly centrifuge before running; follow instrument-specific setup and analysis instructions.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030221CA100.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample type and amount should I use for the assay?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse purified genomic DNA from CAR-T, TCR-T or other cells with lentiviral integration. The recommended total DNA per well is between 5 ng and 30 ng; adjust based on preliminary experiments.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is transgene copies per cell calculated?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eCalculate CAR or TCR copies per cell using the formula: CAR or TCR copies\/cell = 2 × (CAR or TCR \/ SCG), where CAR\/TCR and SCG quantities are measured from the standard curve (copies\/μL).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR thermal cycling conditions?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eActivation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 00:15 (40 cycles); Annealing\/Extension 60 °C for 01:00 with fluorescence read during this step.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and control results?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMean Ct-IPC of sample should be within ±1.0 of the NCS Ct-IPC; a significantly higher IPC Ct indicates possible inhibition. NTC and NCS Ct values should be larger than the mean Ct of the lowest standard.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181277864117,"sku":"SK030221CA100","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/CARTCRTransgeneCopyNumberQuantitationKitSHENTEK.jpg?v=1757316229"},{"product_id":"replication-competent-lentivirus-rcl-quantitation-kit-shentek®","title":"Replication-Competent Lentivirus (RCL) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Replication-Competent Lentivirus (RCL) Quantitation Kit is used for the quantitative detection of replicable lentiviral RCL in cell therapy products and gene therapy products produced with lentiviral vectors, such as virus-producing cell banks, end of production cells, viral vectors and CAR-T cells. This kit is rapid, specific and reliable, and can work in coordination with the SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit to quantitate the copy number of replicable lentivirus RCL in samples.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent lentivirus (RCL) in cell therapy and gene therapy products (virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eOne-step reverse transcription quantitative PCR (qRT-PCR) with FAM-labeled probe; ROX passive reference recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided standard curve range (recommended)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard dilutions ST2–ST6: 6 × 10^5, 6 × 10^4, 6 × 10^3, 6 × 10^2, 6 × 10^1 copies\/µL (prepared from ST = 6 × 10^8 copies\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqRT-PCR MIX per reaction 10 µL; add 20 µL sample\/standard\/NTC for a total reaction volume of 30 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003e5×RT-qPCR Buffer 6 µL; RT-qPCR Enzyme MIX 1 µL; RCL Primer\u0026amp;Probe MIX 3 µL; total 10 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReverse transcription: 50 °C 15:00 (1 cycle); Activation: 95 °C 0:30 (1 cycle); 40 cycles: 95 °C 0:15 (denature), 60 °C 1:00 (anneal\/extend, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, Applied Biosystems 7500, LightCycler 480 II, CFX96, qTOWER3 G, etc.).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection\/read settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Threshold setting example: 0.02 (manual Ct), automatic baseline.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be \u0026gt;= 35.00 cycles. NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored at -20 °C; stable for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ dynamic range (inferred from standard curve)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard curve points cover at least 6 × 10^5 to 6 × 10^1 copies\/µL (user should perform validation to determine empirical LOD\/LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate wells recommended for each standard, control and test sample.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e20 µL purified sample per 30 µL reaction (per example workflow); samples must be processed\/extracted (e.g., with SHENTEK Virus DNA \u0026amp; RNA Extraction Kit) prior to qRT-PCR.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRapid and specific quantitation:\u003c\/strong\u003e One-step qRT-PCR assay optimized for accurate quantitation of replication-competent lentivirus (RCL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream sample prep.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, buffer and enzyme mixes; standardized control for construction of a quantitative standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh throughput:\u003c\/strong\u003e Reagents provided for up to 100 reactions and plate-based workflows (96-well format) supported.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eEstablished QC criteria:\u003c\/strong\u003e Includes recommended control acceptance criteria (NTC and NCS Ct thresholds) and example standard curve settings for result interpretation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of replication-competent lentivirus (RCL) in virus-producing cell banks\u003c\/li\u003e\r\n \u003cli\u003eTesting end-of-production cells for RCL contamination\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of RCL in viral vector preparations\u003c\/li\u003e\r\n \u003cli\u003eSafety testing of CAR-T cells and other lentiviral-transduced cell therapy products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and release testing workflows for gene and cell therapy manufacturing (research use)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCL Control (Part No. NNA044), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCL Primer\u0026amp;Probe MIX (Part No. NNC077), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5×RT-qPCR Buffer (Part No. NNC078), 600 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRT-qPCR Enzyme MIX (Part No. NNC079), 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (Part No. NND008), 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (Part No. NND010), 1 mL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; store all components at -20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent RNase contamination: use RNase-free consumables and work areas; thaw reagents at 2–8 °C or on ice.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate benches, micropipettes and tubes for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\r\n \u003cp\u003e• Process Negative Control Sample (NCS) through the same extraction and handling steps as test samples.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard curve points (ST2–ST6) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Verify instrument-specific parameter settings; follow instrument documentation when adapting program parameters.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403441.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403441.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit is intended for research use to quantitatively detect replication-competent lentivirus (RCL) in cell therapy and gene therapy related samples (e.g., virus-producing cell banks, end-of-production cells, viral vectors, CAR-T cells).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What detection instrument and dye settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96). Use FAM as the reporter dye and ROX as the passive reference (if applicable).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended reaction and cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 30 µL reaction (10 µL qRT-PCR MIX + 20 µL sample). Thermal program: 50 °C 15:00 (RT), 95 °C 0:30 (activation), then 40 cycles of 95 °C 0:15 and 60 °C 1:00 (fluorescence read).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve and what is the dynamic range?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the supplied RCL control and perform serial 10-fold dilutions to generate ST2–ST6. Recommended standard concentrations span 6 × 10^5 to 6 × 10^1 copies\/µL; include at least five points for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181705552053,"sku":"1403441","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Lentivirus_RCL_Quantitation_Kit.jpg?v=1742361933"},{"product_id":"replication-competent-retrovirus-rcr-quantitation-kit-shentek®","title":"Replication-Competent Retrovirus (RCR) Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Replication-Competent Retrovirus (RCR) Quantitation Kit is designed for quantitative detection of Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products produced with retrovirus vectors. The kit supports absolute quantitation using a standard-curve qRT-PCR workflow and is compatible with SHENTEK® Virus DNA \u0026amp; RNA Extraction Kit for upstream nucleic acid preparation. The kit provides lyophilized RCR control, primer \u0026amp; probe mix, qRT-PCR buffer and enzyme mix, and reagents sufficient for 100 reactions. Intended for rapid, specific and reliable monitoring of RCR in virus-producing cell banks, end-of-production cells, viral vector preparations and CAR-T cells.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by qRT-PCR using a standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended standard curve points\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST2–ST6 (five concentration points) corresponding to 5 × 10^5, 5 × 10^4, 5 × 10^3, 5 × 10^2, 5 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl stock concentration (after reconstitution)\u003c\/td\u003e\r\n \u003ctd\u003eLabelled RCR Control reconstituted to 5 × 10^8 copies\/μL then serially diluted\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSuggested quantitation range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5 × 10^1 – 5 × 10^5 copies\/μL (based on ST6–ST2 standard points)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection \/ quantitation\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified in manual; lowest standard provided is 5 × 10^1 copies\/μL (ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqRT-PCR mix per well 10 μL + sample 20 μL = total 30 μL reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR component volumes (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003e5×RT-qPCR Buffer 6 μL, RT-qPCR Enzyme MIX 1 μL, RCR Primer\u0026amp;Probe MIX 3 μL (total 10 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 00:30 (1 cycle); 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read during 60 °C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, LightCycler 480 II, CFX96, qTOWER3 G); settings provided for Applied Biosystems 7500 SDS v1.4\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (controls)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of the lowest standard concentration.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at -20 °C; components stable up to 24 months (check labels for expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for RCR quantitation:\u003c\/strong\u003e Specifically targets Replication-Competent Retrovirus (RCR) in cell and gene therapy products, enabling product safety testing and release control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a serially diluted lyophilized RCR control and a standard-curve qRT-PCR method to provide copy number (copies\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete qRT-PCR reagents:\u003c\/strong\u003e Includes primer \u0026amp; probe mix, 5× RT-qPCR buffer, RT-qPCR enzyme mix, RNase-free water and a lyophilized control; reagents provided for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common real-time PCR platforms:\u003c\/strong\u003e Validated instructions and program settings provided for multiple instruments (e.g., ABI 7500, LightCycler 480 II, CFX96).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRNase-free and protected formulations:\u003c\/strong\u003e RNase-free water and light-protected mixes supplied; some components labeled 'protect from light'.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitative detection of RCR in virus-producing cell banks\u003c\/li\u003e\r\n \u003cli\u003eTesting end-of-production cells for RCR contamination\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of RCR in viral vector preparations\u003c\/li\u003e\r\n \u003cli\u003eScreening CAR‑T or other cell therapy products for RCR\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing during gene\/cell therapy manufacturing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for RCR monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCR Control (lyophilized powder)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRCR Primer \u0026amp; Probe MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5× RT-qPCR Buffer\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRT-qPCR Enzyme MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (1.2 mL × 3)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (1 mL × 1)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qRT-PCR (absolute quantitation by standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Work in RNase-free conditions; use RNase-free consumables and reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; store at -20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice. Do not refreeze repeatedly.\u003c\/p\u003e\r\n \u003cp\u003e• Dissolve lyophilized control by adding specified volume of ddH2O and mix thoroughly (vortex\/flick and centrifuge as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and equipment (UV irradiation 30 min and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard curve points (recommended ST2–ST6) and appropriate controls (NTC, NCS).\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35.00 or undetermined; NCS Ct must be larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403442.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403442.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively detect Replication-Competent Retrovirus (RCR) in cell therapy and gene therapy products, including virus-producing cell banks, end-of-production cells, viral vectors and CAR‑T cells.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions does the kit support and what is the reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Each reaction is prepared as a 30 μL total volume (10 μL qRT-PCR mix + 20 μL sample or water), with qRT-PCR mix composed of 6 μL 5×RT-qPCR Buffer, 1 μL RT-qPCR Enzyme MIX and 3 μL RCR Primer\u0026amp;Probe MIX per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the quantitation range and lowest standard provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe recommended standard curve uses five points (ST2–ST6) corresponding to 5 × 10^5 to 5 × 10^1 copies\/μL. The lyophilized RCR Control is reconstituted to 5 × 10^8 copies\/μL and serially diluted to prepare standards.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What instrument settings and acceptance criteria should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal program: RT 50 °C 15:00; Activation 95 °C 00:30; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (read fluorescence at 60 °C). Use absolute quantitation (standard curve). NTC Ct should be ≥ 35 or undetermined; NCS Ct should be larger than mean Ct of the lowest standard.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45181719576757,"sku":"1403442","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Replication-Competent_Retrovirus_RCR_Quantitation_Kit.jpg?v=1742362083"},{"product_id":"rcaav-2-n-quantitation-kit-shentek®","title":"rcAAV-2\/N Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® rcAAV Quantitation Kit is a qPCR-based kit for detection and quantitation of replication-competent adeno-associated virus (rcAAV) contamination in recombinant AAV (rAAV) serotypes designated as rAAV-2\/N. The kit is designed to quantify rcAAV-2\/N contamination in rAAV production samples including harvested cell-culture bulk, purified stocks and final products. The kit contains a T\u0026amp;R DNA control for preparation of an absolute quantitation standard curve, separate Target and Reference Primer \u0026amp; Probe mixes, qPCR reaction buffer and supporting reagents. The kit supports standard-curve (absolute) quantitation and is compatible with common qPCR platforms (e.g., ABI 7500, Bio-Rad CFX96, Roche 480, SHENTEK-96S).\u003c\/p\u003e\n\u003cp\u003eImportant: The ITR sequence of the rAAV test sample must match the provided ITR sequence for correct detection. Reagents supplied are sufficient for 2 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative detection of replication-competent AAV (rcAAV-2\/N) contamination in rAAV-2\/N samples by qPCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions (kit size)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 2 × 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range \/ standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e20 to 2 × 10^6 copies\/µL (standard curve concentrations: ST6=2×10^1 to ST1=2×10^6 copies\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e20 copies\/µL (lowest standard used for quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (10–10.04 µL qPCR mix + 20 µL sample\/standard per well)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling (example ABI 7500)\u003c\/td\u003e\r\n \u003ctd\u003ePre-melt 95 °C 10:00 (1 cycle); Denature 95 °C 00:15; Anneal\/extend 60 °C 00:30; Read at 72 °C 00:30; 40 cycles. (Note: ABI 7500 users set final read step to 72 °C 34 s.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector channels \/ probes\u003c\/td\u003e\r\n \u003ctd\u003eTarget-2 detected in FAM channel; Reference-2 detected in CY5 channel; separate primer\/probe mixes provided\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eReference-2 threshold = 0.06; Target-2 threshold = 0.02; use automatic baseline and manual Ct\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct (Reference and Target) should be \u0026gt;35 or undetermined. NCS Ct values should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eContamination calculation\u003c\/td\u003e\r\n \u003ctd\u003ercAAV contamination rate = (Detection value of Target genes) ÷ (Detection value of Reference genes × dilution factor ÷ 2). Note: each rAAV contains two copies of the Reference gene.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, Bio-Rad CFX96, SHENTEK-96S, Roche 480 and other real-time PCR systems (ROX usage depends on instrument)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for rcAAV-2\/N detection:\u003c\/strong\u003e Specifically optimized to quantify replication-competent AAV contamination in rAAV-2\/N serotypes using ITR-targeted assays.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation by standard curve:\u003c\/strong\u003e Includes T\u0026amp;R DNA Control and dilution scheme to generate a 6-point standard curve (2×10^1 – 2×10^6 copies\/µL) for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete assay components:\u003c\/strong\u003e Provides qPCR reaction buffer, Target and Reference primer\/probe mixes, ROX option, DNA dilution buffer and DNA control for streamlined setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform flexibility:\u003c\/strong\u003e Compatible with multiple real-time PCR instruments; ROX addition is optional depending on instrument model.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProcedural controls and guidance:\u003c\/strong\u003e Includes NTC and NCS guidance, recommended plate layouts, Ct thresholds, and sample dilution recommendations to support robust QC workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of rcAAV contamination in recombinant AAV production (rAAV stocks and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing during rAAV manufacturing\u003c\/li\u003e\r\n \u003cli\u003eAnalysis of harvested cell-culture samples for presence of replication-competent AAV\u003c\/li\u003e\r\n \u003cli\u003eLot release and batch testing for rcAAV contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eT\u0026amp;R DNA Control-2 (lyophilized powder, 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ercAAV qPCR Reaction Buffer (400 µL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eTarget Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReference Primer \u0026amp; Probe MIX-2 (200 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e100× ROX (20 µL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C (recommended)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 4 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eddH2O (1 mL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e2–8 °C (or -20 °C long-term)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 2 × 100 reactions | Method: qPCR (Absolute Quantitation by Standard Curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, lab coat, eye protection, mask) when handling reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-treat surfaces, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Benzonase nuclease treatment of rAAV stocks prior to extraction is required to remove non-encapsidated nucleic acids and improve direct-qPCR accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit instructions for sample extraction when applicable.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use; protect light-sensitive reagents (reaction buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge the lyophilized DNA control at 12,000 rpm for 1 min and reconstitute precisely with 55 µL ddH2O before preparing dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh (recommended six points) and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Add 100× ROX only if required by the qPCR instrument (ABI 7500 requires ROX; SHENTEK-96S and Roche 480 generally do not).\u003c\/p\u003e\r\n \u003cp\u003e• Use recommended Ct threshold settings (Reference = 0.06; Target = 0.02) and check slope\/intercept\/R² of standard curve for assay validity.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403444.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403444.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supports purified rAAV bulk, final product stocks and harvested cell-culture samples. For bulk or final product stocks, perform Benzonase treatment and nucleic acid extraction prior to qPCR. Cell culture samples may be extracted using the SHENTEK Virus DNA \u0026amp; RNA Extraction Kit.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the assay dynamic range and lowest quantifiable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe standard curve covers 2 × 10^1 to 2 × 10^6 copies\/µL (20 to 2,000,000 copies\/µL). The lowest standard used for quantitation (LOQ) is 20 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How is rcAAV contamination rate calculated?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ercAAV contamination rate = (Measured copies of Target gene) ÷ (Measured copies of Reference gene × dilution factor ÷ 2). Note that each rAAV contains two copies of the Reference gene.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Do I need to add ROX to every instrument?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNo. Addition of 100× ROX is instrument-dependent. ABI 7500 requires 0.04 µL ROX per reaction for the Target assay; SHENTEK-96S, Roche 480 and some other systems do not require ROX. Follow your instrument documentation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO). For Research Use Only. Not for use in diagnostic procedures.\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions x 2","offer_id":45181732618421,"sku":"1403444","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/rcAAV-2_N_Quantitation_Kit.jpg?v=1742362203"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/00001_2131c0e4-892d-4190-b31d-2c6fee20c1ea.jpg?v=1775720525","url":"https:\/\/biofargo.com\/collections\/host-cell-residue-testing.oembed","provider":"Biofargo","version":"1.0","type":"link"}