{"title":"Gel Preparation Kits","description":"","products":[{"product_id":"one-step-color-page-gel-rapid-preparation-kit","title":"One step Color PAGE Gel Rapid Preparation Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThis product is designed for the rapid preparation of polyacrylamide gels. It retains Laemmli-like separation characteristics while using a standard Tris-glycine running buffer system.\u003c\/p\u003e\n\u003cp\u003eThis kit adopts the pre-mixed formula of the upper gel and the lower gel. After pouring the Lower gel, the upper gel can be directly poured between the glass plates without waiting for the upper gel to polymerize. The upper gel casted by this kit is colored(red, blue or green) for easy sample loading. The odorless catalyst provided by this kit has excellent stability and polymerization effect, and there is no need to add TEMED in the process of casting gels. The gel casted by this kit can be used for denaturing or native PAGE gel electrophoresis.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eAbility to cast upper and lower gels at the same time\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eColored upper gels(red, blue, green) for easy sample loading\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eOdorless catalyst instead of TEMED and APS\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eImproved resolution for small-size (e.g. 10 kDa) proteins\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; border: 1px solid #999; border-radius: 4px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; min-width: 900px; font-size: 14px; font-family: Helvetica Neue, Helvetica, Arial, sans-serif;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"background: #0056a6; color: #fff; font-weight: bold; text-align: center;\"\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; width: 50%;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; width: 50%;\"\u003eAmount\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003eUpper A\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003eUpper B\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003eLower A\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003eLower B\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"background: #f7f9fb; font-weight: bold; border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003eCatalyst\u003c\/td\u003e\n\u003ctd style=\"border: 1px solid #999999; padding: 10px; text-align: center; width: 50%;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eInput Sample Requirements\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eSimilar to the precast gels, the upper gels cast by this kit do not have the ability of conventional stacking.. However, the bands separated by this gel are clearer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eCatalyst for gel polymerization is affected by the temperature. A higher temperature usually accelerates the polymerization, while lower temperature slows the reaction. The volume of Catalyst could be varied according to your purpose.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eIf the kit is stored at 4°C, please wait until it reaches the room temperature. This could prevent the air bubble production during gels casting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003eThis product is for research use only.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage and Handling\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eCatalyst: Store at -30 ~ -15°C and transport at ≤0°C. It can be stored at 2 ~ 8°C at least 3 months.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOther components: Store at 2 ~ 8°C. Adjust the shipping method according to the destination.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit is stable for at least one year from date of receipt.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Leaflet-One-step_Color_PAGE_Gel_Rapid_Preparation_Kit.pdf?v=1694196030\" target=\"_blank\"\u003eClick here for more guides and user manual.\u003c\/a\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"6% lower gel","offer_id":43102818861237,"sku":"PG210","price":206.0,"currency_code":"USD","in_stock":true},{"title":"7.5% lower gel","offer_id":43102818894005,"sku":"PG211","price":206.0,"currency_code":"USD","in_stock":true},{"title":"10% lower gel","offer_id":43102818926773,"sku":"PG212","price":206.0,"currency_code":"USD","in_stock":true},{"title":"12.5% lower gel","offer_id":43107400319157,"sku":"PG213","price":206.0,"currency_code":"USD","in_stock":true},{"title":"15% lower gel","offer_id":43107400351925,"sku":"PG214","price":206.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/4019040149d787b48eb755b605d5c751.jpg?v=1732878870"},{"product_id":"new-flash-protein-anykd-page-kit","title":"New Flash Protein AnyKD PAGE Kit-Biosci™","description":"\u003ch2\u003eProduct Description\u003c\/h2\u003e\n\u003cp\u003eBioSci™ NewFlash Protein AnyKD PAGE kit is a product with a low acrylamide concentration, and adopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation rate. The kit is easy to use, and prepares gel quickly and safely.\u003c\/p\u003e\n\u003cp\u003eProtein gel preparation is simple, and can be completed in 25 min by mixing resolving gels and stacking gels at the same time.\u003c\/p\u003e\n\u003cp\u003eProtein gel electrophoresis can be completed either at 300V constant high voltage in about 25 min or at normal voltage.\u003c\/p\u003e\n\u003cp\u003eThe kit is suitable for separation and identification of 10-250 kDa protein, and there is no need to adjust the concentration of resolving gel according to protein molecular weight.\u003cbr\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 72.6632%; height: 108px;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 72.6632%; height: 108px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eStacking Gel SolutionA\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eStacking Gel Solution B\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e50 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eResolving Gel SolutionA\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e125 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eResolving Gel Solution B\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e125 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\" data-mce-style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 37.6049%; height: 18px; text-align: center;\" data-mce-style=\"width: 37.6049%; height: 18px; text-align: center;\"\u003eAmmonium Persulfate(APS)\u003c\/td\u003e\n\u003ctd style=\"width: 33.5344%; height: 18px; text-align: center;\" data-mce-style=\"width: 33.5344%; height: 18px; text-align: center;\"\u003e0.5 g\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eThe number of gels prepared per kit\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 100%;\" data-mce-style=\"border-collapse: collapse; width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e0.75mm Mini-gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e1.0mm Mini-gel \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e\u003cstrong\u003e1.5mm Mini-gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e62 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e50 pieces\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\" data-mce-style=\"width: 33.3333%; text-align: center;\"\u003e33 pieces\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eIt will keep for 12 months if stored away from light at 4℃.\u003c\/p\u003e\n\u003cp\u003eAdd 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS solution, and repack the solution into small portions for storage.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplicability\u003c\/h2\u003e\n\u003cp\u003eFor in vitro study only. Not for use as a diagnostic reagent.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cp\u003e1. This product contains a small amount of acrylamide which is corrosive. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/p\u003e\n\u003cp\u003e2. This product is only used for scientific research by professionals and shall neither be used for clinical diagnosis, treatment, food or drugs nor stored in ordinary housing.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperating Instructions\u003c\/h2\u003e\n\u003cp\u003e1. With BioSci™ NewFlash Protein AnyKD PAGE kit, uniformly mix resolving gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 2.0\/2.5\/3.8 mL of resolving gel solutionsAand B). Gel preparation can be completed in a 15 mL or 50 mL centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e2. With BioSci™ NewFlash Protein AnyKD PAGE kit, uniformly mix stacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 0.8\/1.0\/1.5 mL of stacking gel solutionsAand B).\u003cbr\u003e\u003cstrong\u003eNote: With this product, gelation is rapid. Do not wait for pouring the resolving gel into the glass pates to start the preparation of stacking gel. Excessive waiting time will lead to partial polymerization of the resolving gel, resulting in uneven division surface of resolving gel and stacking gel, which will influence the electrophoresis effect.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e3. Add 10% APS solution to the resolving gel solution in step 1 (for 5 mL of resolving gel solution, add 50 µL of 10% APS solution), and pour the uniformly mixed resolving gel solution into the glass pates to a position 1.5 cm from the top of the front glass plate or 0.5 cm from teeth of a comb.\u003cbr\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Add 10% APS solution to the stacking gel solution in step 2 (for 2 mL of stacking gel solution, add 20 µL of 10% APS solution), and directly pour the uniformly mixed stacking gel solution on the resolving gel solution without waiting for solidification of the resolving gel solution.\u003cbr\u003e\u003cstrong\u003eNote: The kit can complete protein gel casting at one time without mixing stacking gel with resolving gel.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization.\u003cbr\u003e\u003cstrong\u003eNote: Gels can be stored for several weeks at 4℃ in sealing bags with a small amount of electrophoresis buffer.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette for sample loading. It is recommended that BioSci™ ColorBand Prestained Protein Marker (Cat.No.8011021\u0026amp;8011031) shall be used to indicate the molecular weight of the protein samples on PAGE gel.\u003c\/p\u003e\n7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis buffer.\u003cbr\u003eThe voltage can be set to a maximum of 300 V, at which electrophoresis can be completed in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if the current is too high (to avoid much heat).\n\u003cp\u003e\u003cstrong\u003eNote: The electrophoresis of this product can be carried out at normal voltage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"Default Title","offer_id":43348931182773,"sku":"8012011","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012011BioSci_NewFlashProteinAnyKDPAGEKit_1.png?v=1732878254"},{"product_id":"newflash-protein-anykd-color-page-kit-biosci™","title":"NewFlash Protein AnyKD Color PAGE Kit-Biosci™","description":"\u003ch2 data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eProduct Description\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eBiosci™ NewFlash Protein AnyKD Color PAGE kit is a product with a low acrylamide concentration, and adopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation rate. The kit is easy to use, prepares gel quickly and safely, no TEMED reagent, no special smell, and provides color stacking gel (color top gel), which is easy to sample.\u003c\/p\u003e\n\u003cp\u003eProtein gel coagulates quickly, and the entire gel making process can be completed in 25 min.\u003c\/p\u003e\n\u003cp\u003eProtein gel electrophoresis can be completed either at 300 V constant high voltage in about 25 min or at normal voltage.\u003c\/p\u003e\n\u003cp\u003eThe kit is suitable for separation and identification of 10-250 kDa protein, and there is no need to adjust the concentration of resolving gel according to protein molecular weight.\u003c\/p\u003e\n\u003cp\u003eColor stacking gel is easy to sample and remove after electrophoresis.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eStorage and Transportation\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eStored at 2~8℃ protected from light, with a shelf life of 24 months.\u003c\/p\u003e\n\u003cp\u003eTransported on blue ice.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eApplicability\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003eFor Research Use Only.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eProduct Composition\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 81.9686%;\" border=\"1\" data-mce-style=\"border-collapse: collapse; width: 81.9686%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eCat.No.\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003e8012021\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e\n\u003cp\u003e8012031\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e\n\u003cp\u003e8012041\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e\n\u003cp\u003e8012051\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eComponent\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003eSize\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003ePGR\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e\n\u003cp\u003ePGY\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e\n\u003cp\u003ePGG\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e\n\u003cp\u003ePGP\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eStacking\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×50\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e\n\u003cp\u003e√\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eStacking\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×50\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\n\u003cp\u003eRed\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\n\u003cp\u003eYellow\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\n\u003cp\u003eGreen\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\n\u003cp\u003ePurple\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eResolving\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution A\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×125\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eResolving\u003c\/p\u003e\n\u003cp\u003eGel\u003c\/p\u003e\n\u003cp\u003eSolution B\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e1×125\u003c\/p\u003e\n\u003cp\u003emL\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 12.4692%; text-align: center;\" data-mce-style=\"width: 12.4692%; text-align: center;\"\u003e\n\u003cp\u003eAmmoniu\u003c\/p\u003e\n\u003cp\u003em\u003c\/p\u003e\n\u003cp\u003ePersulfate\u003c\/p\u003e\n\u003cp\u003e(APS)\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 10.1235%; text-align: center;\" data-mce-style=\"width: 10.1235%; text-align: center;\"\u003e\n\u003cp\u003e0.5g\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 14.5269%; text-align: center;\" data-mce-style=\"width: 14.5269%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 14.7932%; text-align: center;\" data-mce-style=\"width: 14.7932%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.0274%; text-align: center;\" data-mce-style=\"width: 15.0274%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003ctd style=\"width: 15.2777%; text-align: center;\" data-mce-style=\"width: 15.2777%; text-align: center;\"\u003e√\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eThe number of gels prepared per kit\u003c\/b\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90.2273%; height: 124px;\" data-mce-style=\"border-collapse: collapse; width: 90.2273%; height: 124px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 78px;\" data-mce-style=\"height: 78px;\"\u003e\n\u003ctd style=\"width: 18.1034%; height: 78px; text-align: center;\" data-mce-style=\"width: 18.1034%; height: 78px; text-align: center;\"\u003e \u003c\/td\u003e\n\u003ctd style=\"width: 23.9656%; height: 78px; text-align: center;\" data-mce-style=\"width: 23.9656%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e0.75 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9655%; height: 78px; text-align: center;\" data-mce-style=\"width: 23.9655%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e1.0 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.2759%; height: 78px; text-align: center;\" data-mce-style=\"width: 24.2759%; height: 78px; text-align: center;\"\u003e\n\u003cp\u003e1.5 mm Mini-gel\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 46px;\" data-mce-style=\"height: 46px;\"\u003e\n\u003ctd style=\"width: 18.1034%; height: 46px; text-align: center;\" data-mce-style=\"width: 18.1034%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003eNumber\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9656%; height: 46px; text-align: center;\" data-mce-style=\"width: 23.9656%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e62 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 23.9655%; height: 46px; text-align: center;\" data-mce-style=\"width: 23.9655%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e50 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 24.2759%; height: 46px; text-align: center;\" data-mce-style=\"width: 24.2759%; height: 46px; text-align: center;\"\u003e\n\u003cp\u003e33 pieces\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003ePrecautions\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003e1.This product contains a small amount of acrylamide which is corrosive. For your safety and health, please wear a lab coat and disposable gloves.\u003c\/p\u003e\n\u003cp\u003e2.This product is only used for scientific research by professionals and shall neither be used for clinical diagnosis, treatment, food or drugs nor stored in ordinary housing.\u003c\/p\u003e\n\u003cp\u003e3.\u003cstrong\u003eAdd 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS solution, and repack the solution into small portions for storage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cb\u003eOperating Instructions\u003c\/b\u003e\u003c\/h2\u003e\n\u003cp\u003e1. With Biosci™ NewFlash Protein AnyKD Color PAGE kit, uniformly mix resolving gel\u003cbr\u003esolutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 2.0\/2.5\/3.8 mL of resolving gel solutions A and B). Gel preparation can be completed in a 15 mL or 50 mL centrifuge tube.\u003c\/p\u003e\n\u003cp\u003e2. With Biosci™ NewFlash Protein AnyKD Color PAGE kit, uniformly mix stacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw 0.8\/1.0\/1.5 mL of stacking gel solutions A and B).\u003c\/p\u003e\n\u003cp\u003e3. Add 10% APS solution to the resolving gel solution in step 1 (for 5 mL of resolving gel solution, add 50 µL of 10% APS solution), and pour the uniformly mixed resolving gel solution into the glass plate to a position 1.5 cm from the top of the front glass plate or 0.5 cm from teeth of a comb. Quickly and carefully add water, anhydrous ethanol, or isopropyl alcohol to cover the resolving gel solution. Alternatively, you can proceed directly to Step 5 without sealing the resolving gel solution, so that the liquid level of the resolving gel solution and the stacking gel solution is not very smooth, which is a normal phenomenon, does not affect the electrophoretic results. \u003cbr\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. After the resolving gel solution has solidified (3~10 min), pour out the covering liquid, wash the top of the resolving gel solution with deionized water for several times to remove the unpolymerized gel, remove the top liquid, and absorb the residual deionized water with filter paper.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e5. Add 10% APS solution to the stacking gel solution in step 2 (for 2 mL of stacking gel solution, add 20 µL of 10% APS solution), and directly pour the uniformly mixed stacking gel solution on the resolving gel solution. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization. Note: Gels can be stored for several weeks at 2~8℃ in sealing bags with a small amount of electrophoresis buffer.\u003c\/p\u003e\n\u003cp\u003e6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette for sample loading.\u003c\/p\u003e\n\u003cp\u003e7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis buffer. The voltage can be set to a maximum of 300 V, at which electrophoresis can be completed in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if the current is too high (to avoid much heat). Note: The electrophoresis of this product can be carried out at normal voltage.\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"Default Title","offer_id":43399030374581,"sku":"8012031","price":90.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012031BioSci_NewFlashProteinAnyKDColorPAGEKit_1.png?v=1732878264"},{"product_id":"newflash-protein-low-mw-page-gel-dakewe","title":"NewFlash Protein Low MW PAGE Gel - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eModel \u0026amp; Size\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-04_16-05-32_600x600.png?v=1717488358\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: start;\"\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eNewFlash Protein Low MW PAGE Gel kit is a product with a low acrylamide concentration, and \u003c\/span\u003e\u003cspan\u003eadopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation \u003c\/span\u003e\u003cspan\u003erate. The kit is easy to use, and prepares gel quickly and safely. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtein gel preparation is simple, and can be completed in 25 minutes by mixing resolving gels and \u003c\/span\u003e\u003cspan\u003estacking gels at the same time. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtein gel electrophoresis can be completed either at 300 V constant high voltage in about 45 \u003c\/span\u003e\u003cspan\u003eminutes or at normal voltage. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit is suitable for separation and identification of 1-80 kDa protein, and for separation and \u003c\/span\u003e\u003cspan\u003eidentification of low molecular weight proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eThe number of gels prepared per kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-04_16-08-09_600x600.png?v=1717488509\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. With NewFlash Protein Low MW PAGE Gel kit ,niformly mix low molecular weight resolving \u003c\/span\u003e\u003cspan\u003egel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively \u003c\/span\u003e\u003cspan\u003edraw 2.0\/2.5\/3.8 mL of low molecular weight resolving gel solutions A and B). Gel preparation \u003c\/span\u003e\u003cspan\u003ecan be completed in a 15 mL or 50 mL centrifuge tube. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. With NewFlash Protein AnyKD PAGE Gel kit , uniformly mix low molecular weight stacking gel \u003c\/span\u003e\u003cspan\u003esolutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, respectively draw \u003c\/span\u003e\u003cspan\u003e0.8\/1.0\/1.5 mL of low molecular weight stacking gel solutions A and B).\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cp\u003e\u003cstrong\u003eNote: With this product, gelation is rapid. Do not wait for pouring the resolving gel into the glass plate to start the preparation of stacking gel. Excessive waiting time will lead to partial polymerization of the resolving gel, resulting in uneven division surface of resolving gel and stacking gel, which will influence the electrophoresis effect. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Add 10% APS solution to the low molecular weight resolving gel solution in step 1 (for 5 mL of \u003c\/span\u003e\u003cspan\u003elow molecular weight resolving gel solution, add 50 µL of 10% APS solution), and pour the \u003c\/span\u003e\u003cspan\u003euniformly mixed low molecular weight resolving gel solution into the glass plate to a position 1.5 \u003c\/span\u003e\u003cspan\u003ecm from the top of the front glass plate or 0.5 cm from teeth of a comb. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Add 10% APS solution to the low molecular weight stacking gel solution in step 2 (for 2 mL of \u003c\/span\u003e\u003cspan\u003elow molecular weight stacking gel solution, add 20 µL of 10% APS solution), and directly pour \u003c\/span\u003e\u003cspan\u003ethe uniformly mixed low molecular weight stacking gel solution on the resolving gel solution \u003c\/span\u003e\u003cspan\u003ewithout waiting for solidification of the resolving gel solution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The kit can complete protein gel casting at one time without mixing stacking gel with resolving gel. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Insert the comb into the gel, and keep it standing for 15-20 minutes to wait for gel \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003epolymerization. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Gels can be stored for several weeks at 2~8℃ in sealing bags with a small amount of electrophoresis buffer. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. After gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a pipette \u003c\/span\u003e\u003cspan\u003efor sample loading. It is recommended that low molecular weight protein marker shall be used to \u003c\/span\u003e\u003cspan\u003eindicate the molecular weight of the protein samples on PAGE. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7. The electrophoresis of this product can be carried out rapidly in a conventional electrophoresis \u003c\/span\u003e\u003cspan\u003ebuffer. The voltage can be set to a maximum of 300 V, at which electrophoresis can be \u003c\/span\u003e\u003cspan\u003ecompleted in about 45 minutes. The current shall not exceed 140 mA. The voltage can be reduced if \u003c\/span\u003e\u003cspan\u003ethe current istoo high (to avoid much heat). \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The electrophoresis of this product can be carried out at normal voltage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. This product contains a small amount of acrylamide which is corrosive. For your safety and \u003c\/span\u003e\u003cspan\u003ehealth, please wear a lab coat and disposable gloves. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. This product is only used for scientific research by professionals and shall neither be used for \u003c\/span\u003e\u003cspan\u003eclinical diagnosis, treatment, food or drugs nor stored in ordinary housing. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Add 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS solution, and repack the solution into small portions for storage.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-04_16-16-46_600x600.png?v=1717489023\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStored at 2℃~8℃ protected from light, with a shelf life of 24 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on blue ice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012061_801206T_NewFlash_Protein_Low_MW_PAGE_Gel-_-C3-A4.pdf?v=1717489146\" type=\"application\/pdf\" target=\"_blank\"\u003e NewFlash Protein Low MW PAGE Gel\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012061_NewFlash_Protein_Low_MW_PAGE-MSDS-_-2023.10.23.pdf?v=1717489146\" type=\"application\/pdf\" target=\"_blank\"\u003e NewFlash Protein Low MW PAGE Gel\u003cspan\u003e-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"50T","offer_id":44242536005813,"sku":"8012061","price":78.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012061_NewFlash_Protein_PAGE_Gel_for_Low_MW.png?v=1732878301"},{"product_id":"newflash-protein-high-mw-page-gel-dakewe","title":"NewFlash Protein High MW PAGE Gel - Biosci™","description":"\u003ch2\u003e\u003cspan\u003eModel \u0026amp; Size\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_13-58-42_600x600.png?v=1717653540\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_13-58-42_600x600.png?v=1717653540\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Description\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eNewFlash Protein High MW PAGE Gel is a product with a low acrylamide concentration, and \u003c\/span\u003e\u003cspan\u003eadopts the latest gel technology to reduce the concentration of acrylamide and enhance the gelation \u003c\/span\u003e\u003cspan\u003erate. The kit is easy to use, and prepares gel quickly and safely. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtein gel preparation is simple, and can be completed in 25 min by mixing resolving gels and \u003c\/span\u003e\u003cspan\u003estacking gels at the same time. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eProtein gel electrophoresis can be completed either at 300 V constant high voltage in about 25 min \u003c\/span\u003e\u003cspan\u003eor at normal voltage. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eThe kit is suitable for separation and identification of 50-300 kDa protein, and for separation and \u003c\/span\u003e\u003cspan\u003eidentification of high molecular weight proteins.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eThe number of gels prepared per kit\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-01-38_600x600.png?v=1717653713\" style=\"margin-bottom: 16px; float: none;\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-01-38_600x600.png?v=1717653713\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eOperating Instructions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. With NewFlash Protein High MW PAGE Gel kit, uniformly mix high molecular weight \u003c\/span\u003e\u003cspan\u003eresolving gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, \u003c\/span\u003e\u003cspan\u003erespectively draw 2.0\/2.5\/3.8 mL of high molecular weight resolving gel solutions A and B). Gel \u003c\/span\u003e\u003cspan\u003epreparation can be completed in a 15 mL or 50 mL centrifuge tube. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. With NewFlash Protein High MW PAGE Gel kit, uniformly mix high molecular weight \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003estacking gel solutions A and B at a ratio of 1:1 (As a reference, for a 0.75\/1.0\/1.5 mm gel, \u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\n\u003cp\u003e\u003cspan\u003erespectively draw 0.8\/1.0\/1.5 mL of high molecular weight stacking gel solutions A and B).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: With this product, gelation is rapid. Do not wait for pouring the resolving gel into the glass plate to start the preparation of stacking gel. Excessive waiting time will lead to partial polymerization of the resolving gel, resulting in uneven division surface of resolving gel and stacking gel, which will influence the electrophoresis effect. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. Add 10% APS solution to the high molecular weight resolving gel solution in step 1 (for 5 mL \u003c\/span\u003e\u003cspan\u003eof high molecular weight resolving gel solution, add 50 µL of 10% APS solution), and pour the \u003c\/span\u003e\u003cspan\u003euniformly mixed resolving gel solution into the glass plate to a position 1.5 cm from the top of \u003c\/span\u003e\u003cspan\u003ethe front glass plate or 0.5 cm from teeth of a comb. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: When it is necessary to prepare more than one protein gels at one time, the amount of APS can be appropriately reduced to lower the gelation rate. Excessive APS will result in gel embrittlement. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e4. Add 10% APS solution to the high molecular weight stacking gel solution in step 2 (for 2 mL of \u003c\/span\u003e\u003cspan\u003ehigh molecular weight stacking gel solution, add 20 µL of 10% APS solution), and directly \u003c\/span\u003e\u003cspan\u003epour the uniformly mixed stacking gel solution on the resolving gel solution without waiting for \u003c\/span\u003e\u003cspan\u003esolidification of the resolving gel solution. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The kit can complete protein gel casting at one time without mixing stacking gel with resolving gel. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e5. Insert the comb into the gel, and keep it standing for 15-20 min to wait for gel polymerization. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Gels can be stored for several weeks at 2~8℃ in sealing bags with a small amount of electrophoresis buffer. \u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e6. \u003c\/span\u003e\u003cspan\u003eAfter gel polymerization, remove the comb, and purge gel pores with a 1 mL injector or a \u003c\/span\u003e\u003cspan\u003epipette for sample loading. It is recommended that high molecular weight protein marker shall \u003c\/span\u003e\u003cspan\u003ebe used to indicate the molecular weight of the protein samples on PAGE gel. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e7. \u003c\/span\u003e\u003cspan\u003eThe electrophoresis of this product can be carried out rapidly in a conventional electrophoresis \u003c\/span\u003e\u003cspan\u003ebuffer. The voltage can be set to a maximum of 300 V, at which electrophoresis can be \u003c\/span\u003e\u003cspan\u003ecompleted in about 25 min. The current shall not exceed 140 mA. The voltage can be reduced if \u003c\/span\u003e\u003cspan\u003ethe current is too high (to avoid much heat). \u003c\/span\u003e\u003cspan\u003eNote: The electrophoresis of this product can be carried \u003c\/span\u003e\u003cspan\u003eout at normal voltage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePrecautions\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e1. This product contains a small amount of acrylamide which is corrosive. For your safety and \u003c\/span\u003e\u003cspan\u003ehealth, please wear a lab coat and disposable gloves. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e2. This product is only used for scientific research by professionals and shall neither be used for \u003c\/span\u003e\u003cspan\u003eclinical diagnosis, treatment, food or drugs nor stored in ordinary housing. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003e3. \u003c\/span\u003e\u003cspan\u003eAdd 5 mL of deionized water to the ammonium persulfate (APS) tube to prepare 10% APS \u003c\/span\u003e\u003cspan\u003esolution, and repack the solution into small portions for storage.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eDescription of Product Symbol\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-07-07_600x600.png?v=1717654049\" style=\"margin-bottom: 16px; float: none;\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-06-06_14-07-07_600x600.png?v=1717654049\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\" data-mce-style=\"text-align: left;\"\u003e\n\u003ch2\u003e\u003cspan\u003eStorage and Transportation\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStored at 2℃~8℃ protected from light, with a shelf life of 24 months. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTransported on blue ice.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012071-801207T_NewFlash_Protein_High_MW_PAGE_Gel-_-C3-A4.pdf?v=1717654161\" data-mce-href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012071-801207T_NewFlash_Protein_High_MW_PAGE_Gel-_-C3-A4.pdf?v=1717654161\" type=\"application\/pdf\" target=\"_blank\"\u003eNewFlash Protein High MW PAGE Gel\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012071_NewFlash_Protein_High_MW_PAGE-MSDS-_-2023.10.23.pdf?v=1717654161\" data-mce-href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8012071_NewFlash_Protein_High_MW_PAGE-MSDS-_-2023.10.23.pdf?v=1717654161\" type=\"application\/pdf\" target=\"_blank\"\u003eNewFlash Protein High MW PAGE Gel\u003cspan\u003e-MSDS\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c!----\u003e","brand":"Dakewe","offers":[{"title":"1Kit","offer_id":44250311131317,"sku":"8012071","price":78.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8012071_NewFlash_Protein_PAGE_Gel_for_High_MW_97847466-cef3-48a1-bfbe-51b2276d35d7.png?v=1732878276"},{"product_id":"6-one-step-page-color-gel-quick-preparation-kit","title":"6% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation often requires multiple preparation steps, including accurate acrylamide formulation, separate stacking and resolving gel preparation, APS activation, and TEMED addition. These manual steps increase preparation time and introduce variability between experiments, especially when consistent protein separation performance is required for Western blotting and protein characterization workflows.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e6% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0221)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for rapid preparation of low-percentage polyacrylamide gels used in \u003cstrong\u003eprotein electrophoresis\u003c\/strong\u003e applications. The kit provides pre-optimized separation gel and concentrating gel solutions together with a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complicated reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, this \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e simplifies the gel casting workflow while maintaining reliable protein separation performance. The 6% polyacrylamide gel concentration provides a larger pore size suitable for resolving high molecular weight proteins, protein complexes, and samples requiring improved migration of large proteins.\u003c\/p\u003e\n\u003cp\u003eThe prepared concentrating gel contains a visible color indicator, helping researchers easily identify loading wells and improve sample loading accuracy. Compared with conventional hand-cast SDS-PAGE gels, this \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation solution\u003c\/strong\u003e reduces preparation complexity and improves workflow consistency for laboratories performing routine protein analysis.\u003c\/p\u003e\n\u003cp\u003eThe RP0221 kit is intended for research use only and is suitable for applications including \u003cstrong\u003eWestern blot gel preparation, protein expression analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e6% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0221\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e6%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, Western blotting, protein separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-formulated gel solutions eliminate complex concentration calculations and simplify polyacrylamide gel casting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free gel preparation:\u003c\/strong\u003e The optimized coagulant system replaces traditional TEMED-assisted polymerization workflows, reducing handling of additional polymerization reagents.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid PAGE gel preparation:\u003c\/strong\u003e Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eColor-coded concentrating gel:\u003c\/strong\u003e The colored stacking gel improves visualization of loading wells and supports accurate sample application.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized for high molecular weight proteins:\u003c\/strong\u003e The 6% SDS-PAGE gel provides larger pore sizes suitable for separation of large proteins and protein complexes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein separation:\u003c\/strong\u003e Pre-optimized reagent formulation helps reduce variation caused by manual preparation procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe 6% One-Step SDS-PAGE Color Gel Fast Preparation Kit is designed for laboratories requiring reliable separation of high molecular weight proteins using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for protein transfer and immunodetection workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh molecular weight protein separation:\u003c\/strong\u003e Suitable for resolving large proteins that require lower percentage polyacrylamide gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein expression analysis:\u003c\/strong\u003e Evaluation of recombinant protein expression and purification results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein characterization:\u003c\/strong\u003e Analysis of purified proteins, protein complexes, and molecular weight distribution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiotechnology research:\u003c\/strong\u003e Routine protein electrophoresis workflows in molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAcademic and teaching laboratories:\u003c\/strong\u003e Simplified SDS-PAGE gel preparation for training and educational experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eThe optimal SDS-PAGE gel concentration depends on protein molecular weight and desired separation resolution. Lower percentage polyacrylamide gels contain larger pores and are commonly selected for high molecular weight proteins, while higher percentage gels provide better resolution for smaller proteins.\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellspacing=\"0\" cellpadding=\"8\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e6% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/8-sds-page-gel-preparation-kit?variant=52775642595509\"\u003e8% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/10-sds-page-gel-preparation-kit?variant=52775673626805\"\u003e10% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/12-sds-page-gel-preparation-kit?variant=52775704723637\"\u003e12% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/15-sds-page-gel-preparation-kit?variant=52775732707509\"\u003e15% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nWORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0221 kit provides a simplified protein gel casting workflow compared with conventional SDS-PAGE gel preparation methods.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to initiate gel polymerization and gently mix to avoid bubble formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the resolving gel mixture into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare the concentrating gel solution by mixing Concentrating Gel Solution and Concentrating Gel Buffer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant and directly cast the concentrating gel without waiting for complete separation gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow approximately 15 minutes for gel polymerization before electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe simplified casting procedure reduces preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE electrophoresis workflows.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0221 kit contains SDS in both separation and concentrating gel buffers and is designed for denaturing SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe prepared concentrating gel functions similarly to commercial precast gels and does not provide the same sample stacking effect as traditional manually prepared stacking gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGel polymerization speed is temperature dependent. Higher temperatures may accelerate polymerization, while lower temperatures may require extended polymerization time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor optimal electrophoresis performance, freshly prepared gels are recommended for use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783473578\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a One-Step SDS-PAGE Gel Preparation Kit?\u003c\/summary\u003e\n\u003cp\u003eA One-Step SDS-PAGE Gel Preparation Kit is a pre-optimized reagent system designed to simplify polyacrylamide gel preparation for protein electrophoresis. Unlike traditional SDS-PAGE gel casting workflows that require separate calculations and addition of multiple polymerization reagents, this kit provides ready-to-use gel solutions and a simplified casting procedure for faster protein gel preparation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a TEMED-free SDS-PAGE gel preparation kit?\u003c\/summary\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation commonly uses TEMED together with APS to initiate acrylamide polymerization. The RP0221 One-Step PAGE Gel Kit uses a novel coagulant system instead of additional TEMED addition, helping simplify gel casting and reduce handling of traditional polymerization reagents.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does it take to prepare an SDS-PAGE gel using RP0221?\u003c\/summary\u003e\n\u003cp\u003eThe RP0221 SDS-PAGE Gel Preparation Kit can complete gel polymerization in approximately 15 minutes under suitable conditions. The one-step workflow eliminates several preparation steps associated with conventional hand-cast PAGE gels.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for a 6% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eA 6% SDS-PAGE gel has larger pore sizes and is recommended for separation of high molecular weight proteins, protein complexes, and samples where improved migration of large proteins is required. It is commonly used for proteins above approximately 100 kDa.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan the RP0221 PAGE gel kit be used for Western blot?\u003c\/summary\u003e\n\u003cp\u003eYes. The RP0221 SDS-PAGE protein gel preparation kit is suitable for Western blot workflows requiring separation of proteins by polyacrylamide gel electrophoresis before transfer to membranes for antibody-based detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0221 compatible with standard SDS-PAGE electrophoresis systems?\u003c\/summary\u003e\n\u003cp\u003eYes. The kit is designed for Tris-glycine SDS-PAGE systems and can be used with standard PAGE electrophoresis equipment used in molecular biology and protein analysis laboratories.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a 6% SDS-PAGE gel and a 10% or 12% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe main difference is pore size. A 6% SDS-PAGE gel provides larger pores suitable for high molecular weight proteins, while 10% and 12% gels are commonly selected for general protein separation and lower molecular weight proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes this kit require APS or TEMED during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eThe RP0221 kit is supplied with optimized gel solutions and a Novel Coagulant system. Additional TEMED is not required during gel preparation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n\"@context\":\"https:\/\/schema.org\",\n\"@type\":\"Product\",\n\"name\":\"6% One-Step SDS-PAGE Color Gel Fast Preparation Kit\",\n\"sku\":\"RP0221\",\n\"description\":\"A rapid SDS-PAGE gel preparation kit designed for protein electrophoresis workflows. The kit provides pre-optimized gel solutions for fast one-step PAGE gel casting without additional TEMED addition.\",\n\"brand\":{\n\"@type\":\"Brand\",\n\"name\":\"AccuRef Scientific\"\n},\n\"category\":\"SDS-PAGE Gel Preparation Kit\",\n\"additionalProperty\":[\n{\n\"@type\":\"PropertyValue\",\n\"name\":\"Gel Concentration\",\n\"value\":\"6%\"\n},\n{\n\"@type\":\"PropertyValue\",\n\"name\":\"Application\",\n\"value\":\"Protein electrophoresis, Western blotting, polyacrylamide gel electrophoresis\"\n},\n{\n\"@type\":\"PropertyValue\",\n\"name\":\"Gel System\",\n\"value\":\"Tris-Glycine SDS-PAGE\"\n}\n]\n}\n\u003c\/script\u003e \u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e \u003cscript type=\"application\/ld+json\"\u003e\n{\n\"@context\":\"https:\/\/schema.org\",\n\"@type\":\"FAQPage\",\n\"mainEntity\":[\n\n{\n\"@type\":\"Question\",\n\"name\":\"What is a One-Step SDS-PAGE Gel Preparation Kit?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"A One-Step SDS-PAGE Gel Preparation Kit is a pre-optimized reagent system designed to simplify polyacrylamide gel preparation for protein electrophoresis.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"What are the advantages of a TEMED-free SDS-PAGE gel preparation kit?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"TEMED-free SDS-PAGE gel preparation simplifies gel casting by reducing additional polymerization reagent handling requirements.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"How long does it take to prepare an SDS-PAGE gel using RP0221?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"The RP0221 kit can complete gel polymerization in approximately 15 minutes under suitable conditions.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"Can RP0221 be used for Western blot?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"Yes. RP0221 is suitable for SDS-PAGE protein separation workflows used before Western blot analysis.\"\n}\n},\n\n{\n\"@type\":\"Question\",\n\"name\":\"What proteins are suitable for a 6% SDS-PAGE gel?\",\n\"acceptedAnswer\":{\n\"@type\":\"Answer\",\n\"text\":\"A 6% SDS-PAGE gel is recommended for high molecular weight proteins and protein complexes requiring larger pore sizes.\"\n}\n}\n\n]\n}\n\u003c\/script\u003e\u003c\/section\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775633911989,"sku":"RP0221","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783476533"},{"product_id":"8-sds-page-gel-preparation-kit","title":"8% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation requires multiple manual steps, including gel percentage calculation, separate preparation of stacking and resolving gels, polymerization reagent handling, and repeated optimization between experiments. These procedures can increase preparation time and introduce variation in gel quality, especially when laboratories require consistent protein separation results for Western blotting and protein characterization.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e8% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0231)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for rapid preparation of medium-percentage polyacrylamide gels used in \u003cstrong\u003eprotein electrophoresis\u003c\/strong\u003e applications. The kit contains pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, enabling researchers to prepare PAGE gels without additional TEMED addition or complex reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, the RP0231 \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e provides a simplified gel casting workflow for laboratories analyzing medium to high molecular weight proteins. The 8% polyacrylamide gel concentration offers an optimized pore structure for resolving proteins in the approximately 50–200 kDa range, providing improved separation performance compared with lower percentage gels when analyzing moderately sized proteins.\u003c\/p\u003e\n\u003cp\u003eThe colored concentrating gel included in this \u003cstrong\u003eprotein gel casting kit\u003c\/strong\u003e helps visualize loading wells and improves sample loading accuracy. By combining pre-mixed gel preparation reagents with a TEMED-free polymerization workflow, the kit reduces preparation complexity and improves consistency compared with conventional hand-cast SDS-PAGE gels.\u003c\/p\u003e\n\u003cp\u003eThe RP0231 kit is suitable for research workflows including \u003cstrong\u003eWestern blot gel preparation, recombinant protein analysis, protein expression studies, and polyacrylamide gel electrophoresis\u003c\/strong\u003e. It provides a practical alternative for laboratories seeking a faster and more reproducible SDS-PAGE gel preparation solution.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0231\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, Western blotting, protein separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized 8% SDS-PAGE gel formulation:\u003c\/strong\u003e Provides suitable pore size and separation performance for medium to high molecular weight protein analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-optimized gel solutions simplify polyacrylamide gel casting by reducing manual calculations and preparation steps.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free PAGE gel preparation:\u003c\/strong\u003e The optimized coagulant system replaces traditional TEMED-assisted workflows, reducing additional reagent handling requirements.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid SDS-PAGE gel preparation:\u003c\/strong\u003e Gel polymerization can be completed in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eColor-coded concentrating gel:\u003c\/strong\u003e The visible stacking gel indicator improves identification of loading wells and supports accurate sample application.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eReliable protein separation:\u003c\/strong\u003e The pre-formulated system helps improve batch-to-batch consistency compared with manually prepared SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e8% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/strong\u003e is designed for researchers requiring reproducible separation of medium to high molecular weight proteins using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for protein transfer and antibody-based detection workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMedium and high molecular weight protein separation:\u003c\/strong\u003e Suitable for proteins requiring an 8% polyacrylamide gel matrix for improved migration and resolution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein characterization:\u003c\/strong\u003e Analysis of recombinant proteins, purified proteins, and protein expression products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein expression analysis:\u003c\/strong\u003e Evaluation of protein expression levels from bacterial, mammalian, or other biological expression systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein electrophoresis workflows:\u003c\/strong\u003e Routine SDS-PAGE applications in biotechnology and molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eResearch and teaching laboratories:\u003c\/strong\u003e Simplified PAGE gel preparation for laboratories requiring efficient and consistent electrophoresis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eSelecting the appropriate SDS-PAGE gel concentration is essential for achieving optimal protein separation. Lower percentage polyacrylamide gels provide larger pore sizes for large proteins, while higher percentage gels improve resolution for smaller molecular weight proteins. The \u003cstrong\u003e8% SDS-PAGE gel\u003c\/strong\u003e provides a balanced separation range for medium to high molecular weight proteins and is commonly used in protein electrophoresis and Western blot workflows.\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellspacing=\"0\" cellpadding=\"8\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/6-one-step-page-color-gel-quick-preparation-kit?variant=52775633911989\"\u003e6% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e8% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/10-sds-page-gel-preparation-kit?variant=52775673626805\"\u003e10% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/12-sds-page-gel-preparation-kit?variant=52775704723637\"\u003e12% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/15-sds-page-gel-preparation-kit?variant=52775732707509\"\u003e15% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPREPARATION WORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0231 \u003cstrong\u003eOne-Step SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e simplifies traditional PAGE gel casting by combining optimized gel solutions with a rapid polymerization system. The workflow reduces preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE electrophoresis systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd the Novel Coagulant to the separation gel mixture and gently mix to initiate polymerization while avoiding bubble formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the prepared separation gel solution into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix Concentrating Gel Solution and Concentrating Gel Buffer in equal volumes to prepare the stacking gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the concentrating gel mixture and directly load it above the separation gel layer without waiting for complete resolving gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow the gel to polymerize for approximately 15 minutes before performing SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThis rapid gel preparation workflow provides a convenient alternative to conventional hand-cast SDS-PAGE gels while reducing preparation variation between experiments.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0231 kit is formulated for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e. Both separation gel and concentrating gel buffers contain SDS and are not intended for native PAGE applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe prepared concentrating gel provides a loading-friendly colored interface and is designed to simplify sample loading. Its performance is similar to commercial precast gel systems rather than traditional high-concentration stacking gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGel polymerization speed may vary depending on ambient temperature. Higher temperatures can accelerate polymerization, while lower temperatures may require additional polymerization time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor consistent protein separation performance, freshly prepared gels are recommended for electrophoresis experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe 8% polyacrylamide formulation is particularly suitable for samples requiring improved migration of medium to high molecular weight proteins compared with higher percentage SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e \u003cstrong\u003eManual\u003c\/strong\u003e \u003c\/a\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783474376\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003ca href=\"#\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is an 8% SDS-PAGE Gel Preparation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eAn 8% SDS-PAGE Gel Preparation Kit is designed for preparing polyacrylamide gels suitable for medium to high molecular weight protein separation. The RP0231 kit is commonly used for protein electrophoresis, Western blot sample preparation, recombinant protein analysis, and molecular biology research workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy choose an 8% SDS-PAGE gel instead of a 6% or 10% gel?\u003c\/summary\u003e\n\u003cp\u003eAn 8% SDS-PAGE gel provides an intermediate pore size between lower percentage gels designed for very large proteins and higher percentage gels optimized for smaller proteins. It is suitable for researchers who need balanced migration and resolution for proteins approximately 50–200 kDa.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RP0231 require TEMED or APS during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eNo additional TEMED addition is required. The RP0231 kit contains optimized gel preparation reagents and a Novel Coagulant system designed to simplify SDS-PAGE gel casting compared with traditional polymerization workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0231 be used for Western blot experiments?\u003c\/summary\u003e\n\u003cp\u003eYes. The RP0231 SDS-PAGE protein gel preparation kit is compatible with Western blot workflows where proteins are separated by polyacrylamide gel electrophoresis before membrane transfer and antibody detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat protein molecular weight range is recommended for an 8% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0231 8% SDS-PAGE gel is recommended for medium to high molecular weight proteins, typically around 50–200 kDa, depending on electrophoresis conditions and sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a one-step PAGE gel casting kit?\u003c\/summary\u003e\n\u003cp\u003eA one-step PAGE gel casting kit reduces preparation steps by combining optimized gel solutions and simplifying polymerization procedures. This helps laboratories improve workflow efficiency and reduce variability caused by manual reagent preparation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0231 compatible with standard Tris-glycine SDS-PAGE systems?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0231 is designed for conventional Tris-glycine SDS-PAGE electrophoresis systems and can be used with standard PAGE gel casting and electrophoresis equipment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between RP0231 and other One-Step SDS-PAGE Gel Kits?\u003c\/summary\u003e\n\u003cp\u003eThe main difference between products in the series is gel concentration. RP0231 provides an 8% gel formulation optimized for medium to high molecular weight protein separation, while other concentrations are designed for different protein size ranges.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775642595509,"sku":"RP0231","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783476426"},{"product_id":"10-sds-page-gel-preparation-kit","title":"10% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation requires multiple manual steps, including gel concentration calculations, separate preparation of resolving and stacking gels, polymerization reagent handling, and optimization of casting conditions. These additional procedures may increase experimental preparation time and introduce variation that affects protein separation consistency, especially in routine Western blot and protein expression analysis workflows.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e10% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0241)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for routine protein electrophoresis applications. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system to simplify polyacrylamide gel preparation without additional TEMED addition or complicated reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, the RP0241 \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e provides a balanced 10% polyacrylamide gel formulation suitable for general protein separation and commonly used \u003cstrong\u003eWestern blot gel preparation\u003c\/strong\u003e workflows. The 10% SDS-PAGE gel concentration offers a versatile pore size for resolving a broad range of commonly analyzed proteins, making it a preferred choice for routine protein expression analysis and molecular biology applications.\u003c\/p\u003e\n\u003cp\u003eThe colored concentrating gel included in this \u003cstrong\u003eprotein gel casting kit\u003c\/strong\u003e improves visualization of sample wells and supports accurate loading during electrophoresis. By integrating optimized gel preparation reagents with a \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation workflow\u003c\/strong\u003e, RP0241 helps laboratories reduce preparation complexity while improving consistency between experiments.\u003c\/p\u003e\n\u003cp\u003eThe RP0241 kit is intended for research use only and is suitable for applications including \u003cstrong\u003eWestern blot analysis, protein expression detection, recombinant protein characterization, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0241\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e10%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, Western blotting, protein expression analysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellpadding=\"8\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized 10% SDS-PAGE gel formulation:\u003c\/strong\u003e Provides a balanced pore structure suitable for routine protein separation and commonly analyzed molecular weight ranges.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eIdeal for routine Western blot workflows:\u003c\/strong\u003e The 10% gel concentration offers versatile separation performance for frequently studied proteins in molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-formulated gel solutions simplify polyacrylamide gel casting by reducing manual preparation steps and concentration calculations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free PAGE gel preparation:\u003c\/strong\u003e The optimized coagulant system eliminates the need for additional TEMED addition during gel preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid SDS-PAGE gel preparation:\u003c\/strong\u003e Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein separation:\u003c\/strong\u003e Pre-optimized reagents help reduce variability associated with conventional hand-cast SDS-PAGE gel preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e10% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/strong\u003e is designed for laboratories requiring reliable and versatile protein separation performance for routine \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e workflows.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for routine protein transfer and antibody-based detection experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRoutine protein expression analysis:\u003c\/strong\u003e Evaluation of protein expression levels from bacterial, mammalian, and other biological expression systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein characterization:\u003c\/strong\u003e Analysis of purified recombinant proteins and protein expression products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein molecular weight analysis:\u003c\/strong\u003e Determination of relative protein size and comparison of protein expression profiles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell lysate protein analysis:\u003c\/strong\u003e Separation of proteins from biological samples for downstream detection and characterization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiotechnology and academic research:\u003c\/strong\u003e A convenient SDS-PAGE gel preparation solution for routine laboratory protein analysis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eThe appropriate SDS-PAGE gel concentration depends on protein molecular weight, sample complexity, and the desired separation resolution. Lower percentage gels provide larger pores for high molecular weight proteins, while higher percentage gels improve resolution for smaller proteins. The \u003cstrong\u003e10% SDS-PAGE gel\u003c\/strong\u003e is widely used as a general-purpose protein separation gel because it provides balanced migration and resolution across many routine Western blot and protein analysis applications.\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellspacing=\"0\" cellpadding=\"8\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/6-one-step-page-color-gel-quick-preparation-kit?variant=52775633911989\"\u003e6% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/8-sds-page-gel-preparation-kit?variant=52775642595509\"\u003e8% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e10% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/12-sds-page-gel-preparation-kit?variant=52775704723637\"\u003e12% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/15-sds-page-gel-preparation-kit?variant=52775732707509\"\u003e15% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPREPARATION WORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0241 \u003cstrong\u003eOne-Step SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e provides a simplified protein gel casting workflow compared with conventional SDS-PAGE preparation methods. Pre-optimized solutions reduce manual preparation steps while maintaining compatibility with standard Tris-glycine electrophoresis systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel mixture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the separation gel mixture and gently mix to initiate polymerization while avoiding bubble formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the prepared separation gel solution into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix Concentrating Gel Solution and Concentrating Gel Buffer in equal volumes to prepare the stacking gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the concentrating gel mixture and directly cast the stacking gel above the separation gel layer without waiting for complete resolving gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow approximately 15 minutes for gel polymerization before starting SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe simplified casting procedure improves workflow efficiency for routine protein electrophoresis experiments while reducing variability associated with traditional manual PAGE gel preparation.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0241 kit is designed for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e. Separation and concentrating gel buffers contain SDS and are not intended for native PAGE applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe 10% polyacrylamide gel formulation provides a balanced separation range suitable for many commonly analyzed proteins in Western blot and protein expression studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe colored concentrating gel helps researchers identify loading wells during sample application and improves loading consistency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGel polymerization speed is affected by temperature. Higher temperatures may accelerate polymerization, while lower temperatures may require additional polymerization time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor optimal protein separation performance, freshly prepared SDS-PAGE gels are recommended for electrophoresis experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e \u003cstrong\u003eManual\u003c\/strong\u003e \u003c\/a\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783474376\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003ca href=\"#\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 10% SDS-PAGE gel commonly used for protein analysis?\u003c\/summary\u003e\n\u003cp\u003eA 10% SDS-PAGE gel provides a balanced pore size that allows effective separation of many commonly studied proteins. It is widely used for routine Western blotting, protein expression analysis, and general protein electrophoresis because it covers a broad molecular weight range.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for RP0241 10% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0241 10% SDS-PAGE gel is suitable for proteins in the approximate range of 20–150 kDa, depending on electrophoresis conditions and sample characteristics. It is commonly selected for routine protein separation workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0241 be used for Western blot gel preparation?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0241 is designed for SDS-PAGE protein separation before membrane transfer and antibody detection. The 10% gel concentration provides versatile separation performance for many Western blot applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a one-step SDS-PAGE gel preparation kit?\u003c\/summary\u003e\n\u003cp\u003eA one-step SDS-PAGE gel preparation kit simplifies traditional gel casting by providing pre-optimized solutions and reducing the need for multiple preparation steps. This helps improve laboratory efficiency and reproducibility.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RP0241 require additional TEMED during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eNo. RP0241 uses an optimized coagulant system and does not require additional TEMED addition during the gel preparation process, simplifying SDS-PAGE gel casting.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0241 compatible with standard SDS-PAGE electrophoresis equipment?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0241 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between RP0241 10% gel and RP0231 8% gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0231 8% SDS-PAGE gel is optimized for medium to high molecular weight proteins, while RP0241 10% SDS-PAGE gel provides a more balanced separation range for routine protein analysis and Western blot workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0241 replace traditional hand-cast SDS-PAGE gels?\u003c\/summary\u003e\n\u003cp\u003eRP0241 provides a simplified alternative to conventional hand-cast SDS-PAGE gel preparation by combining optimized gel solutions with a rapid polymerization workflow while maintaining compatibility with standard protein electrophoresis applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775673626805,"sku":"RP0241","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783476252"},{"product_id":"12-sds-page-gel-preparation-kit","title":"12% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation involves multiple manual procedures, including accurate acrylamide concentration adjustment, separate preparation of stacking and resolving gels, polymerization reagent handling, and repeated optimization for different protein samples. These steps may increase preparation time and introduce variation in gel performance, particularly when researchers require consistent resolution of lower molecular weight proteins and routine protein analysis results.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e12% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0251)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for efficient preparation of medium-to-high percentage polyacrylamide gels used in \u003cstrong\u003eprotein electrophoresis\u003c\/strong\u003e applications. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complicated reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, the RP0251 \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e provides a 12% polyacrylamide gel formulation optimized for routine protein separation requiring improved resolution of lower molecular weight proteins compared with lower percentage gels. The balanced pore structure makes it suitable for common Western blot workflows, protein expression analysis, and molecular weight characterization.\u003c\/p\u003e\n\u003cp\u003eThe colored concentrating gel included in this \u003cstrong\u003eprotein gel casting kit\u003c\/strong\u003e improves visualization of sample loading wells and supports accurate sample application during electrophoresis. Compared with conventional hand-cast SDS-PAGE methods, the \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation workflow\u003c\/strong\u003e reduces preparation complexity and improves consistency between experiments.\u003c\/p\u003e\n\u003cp\u003eThe RP0251 kit is intended for research use only and is suitable for applications including \u003cstrong\u003eWestern blot gel preparation, protein expression analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e12% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0251\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e12%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, Western blotting, protein separation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized 12% SDS-PAGE gel formulation:\u003c\/strong\u003e Provides enhanced separation resolution for routine protein analysis and proteins requiring smaller pore sizes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eImproved resolution for lower molecular weight proteins:\u003c\/strong\u003e The 12% polyacrylamide gel structure supports clearer separation of proteins commonly analyzed in Western blot experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-formulated gel solutions simplify PAGE gel casting by reducing manual calculations and preparation steps.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free gel preparation:\u003c\/strong\u003e The optimized coagulant system simplifies polymerization procedures without additional TEMED addition.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid PAGE gel preparation:\u003c\/strong\u003e Gel polymerization can be completed in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein separation:\u003c\/strong\u003e The pre-optimized formulation helps reduce variability caused by conventional manual SDS-PAGE gel preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e12% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/strong\u003e is designed for laboratories requiring reliable protein separation with improved resolution for routine \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e applications.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for protein transfer and antibody-based detection workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRoutine protein separation:\u003c\/strong\u003e Suitable for resolving commonly studied proteins requiring higher resolution than lower percentage SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein expression analysis:\u003c\/strong\u003e Evaluation of recombinant protein expression and cellular protein profiles.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein characterization:\u003c\/strong\u003e Analysis of purified proteins, protein mixtures, and molecular weight distribution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell lysate analysis:\u003c\/strong\u003e Separation of proteins from biological samples before downstream detection.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiotechnology and academic research:\u003c\/strong\u003e Convenient SDS-PAGE gel preparation for routine molecular biology workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eThe selection of SDS-PAGE gel concentration directly affects protein migration and separation resolution. Lower percentage polyacrylamide gels provide larger pores for high molecular weight proteins, while higher percentage gels provide improved resolution for smaller proteins. The \u003cstrong\u003e12% SDS-PAGE gel\u003c\/strong\u003e offers a smaller pore structure compared with 8% and 10% gels, making it suitable for routine protein separation where enhanced resolution of lower molecular weight proteins is required.\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\" cellspacing=\"0\" cellpadding=\"8\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/6-one-step-page-color-gel-quick-preparation-kit?variant=52775633911989\"\u003e6% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/8-sds-page-gel-preparation-kit?variant=52775642595509\"\u003e8% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/10-sds-page-gel-preparation-kit?variant=52775673626805\"\u003e10% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e12% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/15-sds-page-gel-preparation-kit?variant=52775732707509\"\u003e15% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPREPARATION WORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0251 \u003cstrong\u003eOne-Step SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e provides a simplified gel casting workflow compared with traditional SDS-PAGE preparation procedures. The pre-optimized formulation reduces manual preparation steps while maintaining compatibility with standard Tris-glycine protein electrophoresis systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the separation gel mixture and gently mix to initiate gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the prepared separation gel solution into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare the stacking gel solution by mixing Concentrating Gel Solution and Concentrating Gel Buffer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant and directly cast the concentrating gel above the separation gel layer without waiting for complete resolving gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow approximately 15 minutes for gel polymerization before SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThis rapid PAGE gel preparation workflow helps laboratories improve efficiency and reproducibility for routine protein separation experiments.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0251 kit is designed for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e. Both separation and concentrating gel buffers contain SDS and are not intended for native PAGE workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe 12% polyacrylamide formulation provides increased resolving capability for lower molecular weight proteins compared with lower percentage SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe colored concentrating gel improves visualization of sample loading wells and supports more accurate sample application during electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePolymerization speed may vary depending on environmental temperature. Lower temperatures may require additional polymerization time.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor optimal protein separation performance, freshly prepared gels are recommended before electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e \u003cstrong\u003eManual\u003c\/strong\u003e \u003c\/a\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783474528\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003ca href=\"#\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a 12% SDS-PAGE Gel Preparation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eA 12% SDS-PAGE Gel Preparation Kit is designed for preparing polyacrylamide gels with improved resolution for routine protein separation. RP0251 is commonly used for Western blotting, protein expression analysis, and molecular weight characterization of proteins in the approximate 10–100 kDa range.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy choose a 12% SDS-PAGE gel instead of a 10% gel?\u003c\/summary\u003e\n\u003cp\u003eA 12% SDS-PAGE gel contains a higher acrylamide concentration and smaller pore size compared with a 10% gel. This provides improved resolution for lower molecular weight proteins and samples requiring clearer separation of closely sized protein bands.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0251 be used for Western blot experiments?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0251 is suitable for Western blot workflows requiring SDS-PAGE protein separation before membrane transfer and antibody-based detection. The 12% gel concentration is commonly selected for routine protein analysis with enhanced band resolution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RP0251 require additional TEMED during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eNo. RP0251 contains optimized gel preparation reagents and a Novel Coagulant system that simplifies polymerization without requiring additional TEMED addition during PAGE gel casting.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for a 12% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0251 12% SDS-PAGE gel is suitable for many routine protein analysis applications involving proteins approximately 10–100 kDa. Actual separation performance depends on electrophoresis conditions and sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a One-Step PAGE Gel Kit?\u003c\/summary\u003e\n\u003cp\u003eA One-Step PAGE Gel Kit simplifies SDS-PAGE gel preparation by providing pre-optimized solutions that reduce manual calculations, reagent preparation steps, and variability between experiments.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0251 compatible with standard SDS-PAGE electrophoresis systems?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0251 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between RP0251 12% gel and RP0241 10% gel?\u003c\/summary\u003e\n\u003cp\u003eRP0241 10% SDS-PAGE gel provides a broad and balanced separation range for general protein analysis, while RP0251 12% SDS-PAGE gel offers improved resolution for smaller proteins and routine protein separation requiring higher gel concentration.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775704723637,"sku":"RP0251","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783476064"},{"product_id":"15-sds-page-gel-preparation-kit","title":"15% One-Step PAGE Color Gel Quick Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation requires multiple manual procedures, including precise acrylamide concentration adjustment, separate preparation of resolving and stacking gels, polymerization reagent handling, and repeated optimization for different protein sample types. These steps can increase preparation time and introduce variation, especially when high-resolution separation of small proteins or peptides is required.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit (RP0261)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for high-resolution separation of low molecular weight proteins and peptide samples using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e. The kit provides pre-optimized separation gel solution, concentrating gel solution, corresponding buffers, and a novel coagulant system, allowing researchers to prepare PAGE gels without additional TEMED addition or complex reagent calculations.\u003c\/p\u003e\n\u003cp\u003eDesigned for the \u003cstrong\u003eTris-glycine SDS-PAGE system\u003c\/strong\u003e, the RP0261 \u003cstrong\u003eOne-Step PAGE Gel Kit\u003c\/strong\u003e contains a 15% polyacrylamide gel formulation with smaller pore sizes for improved resolution of small proteins compared with lower percentage SDS-PAGE gels. It is particularly suitable for applications requiring enhanced separation of proteins below approximately 50 kDa, including detailed protein characterization and high-resolution Western blot analysis.\u003c\/p\u003e\n\u003cp\u003eThe colored concentrating gel included in this \u003cstrong\u003eprotein gel casting kit\u003c\/strong\u003e improves visualization of sample loading wells and supports accurate sample application during electrophoresis. Compared with conventional hand-cast SDS-PAGE methods, the \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation workflow\u003c\/strong\u003e reduces preparation complexity and improves consistency for laboratories performing routine and advanced protein analysis.\u003c\/p\u003e\n\u003cp\u003eThe RP0261 kit is intended for research use only and is suitable for applications including \u003cstrong\u003esmall protein separation, peptide electrophoresis, Western blot gel preparation, protein expression analysis, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRP0261\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eProtein electrophoresis, small protein separation, Western blotting\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eOne-step gel casting workflow\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4 °C; Novel Coagulant long-term storage at −20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eConcentrating Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e80 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSeparation Gel Buffer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e250 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Coagulant\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e8 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized 15% SDS-PAGE gel formulation:\u003c\/strong\u003e Provides a high-percentage polyacrylamide matrix with smaller pores for improved separation of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-resolution protein separation:\u003c\/strong\u003e The 15% SDS-PAGE gel improves band resolution for small proteins and peptide samples compared with lower percentage gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-step SDS-PAGE gel preparation:\u003c\/strong\u003e Pre-formulated solutions simplify gel casting by reducing manual calculations and preparation procedures.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free gel preparation:\u003c\/strong\u003e The optimized coagulant system simplifies polymerization without requiring additional TEMED addition during gel preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid PAGE gel preparation:\u003c\/strong\u003e Complete gel polymerization can be achieved in approximately 15 minutes, improving laboratory workflow efficiency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein resolution:\u003c\/strong\u003e The pre-optimized formulation helps reduce variability associated with conventional hand-cast SDS-PAGE gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e15% One-Step SDS-PAGE Color Gel Fast Preparation Kit\u003c\/strong\u003e is designed for laboratories requiring high-resolution separation of small proteins and peptide samples using \u003cstrong\u003epolyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSmall protein separation:\u003c\/strong\u003e Suitable for resolving low molecular weight proteins that require higher percentage SDS-PAGE gels for improved separation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePeptide electrophoresis:\u003c\/strong\u003e Provides enhanced resolution for peptide samples and very small protein fragments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot analysis:\u003c\/strong\u003e Preparation of SDS-PAGE gels for detection of low molecular weight proteins before membrane transfer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein characterization:\u003c\/strong\u003e Analysis of purified proteins, protein fragments, and molecular weight differences.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein expression analysis:\u003c\/strong\u003e Evaluation of recombinant proteins and engineered protein products with lower molecular weights.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBiotechnology and academic research:\u003c\/strong\u003e Convenient high-resolution SDS-PAGE gel preparation for molecular biology laboratories.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eChoosing the Right SDS-PAGE Gel Percentage\u003c\/h2\u003e\n\u003cp\u003eThe optimal SDS-PAGE gel concentration depends on protein molecular weight and the required separation resolution. Lower percentage polyacrylamide gels provide larger pores for high molecular weight proteins, while higher percentage gels provide improved resolution for smaller proteins. The \u003cstrong\u003e15% SDS-PAGE gel\u003c\/strong\u003e contains a higher acrylamide concentration and smaller pore structure, making it suitable for high-resolution separation of small proteins, protein fragments, and peptide samples.\u003c\/p\u003e\n\u003ctable cellpadding=\"8\" cellspacing=\"0\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eRecommended Application\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eTypical Protein Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/6-one-step-page-color-gel-quick-preparation-kit?variant=52775633911989\"\u003e6% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eHigh molecular weight proteins and large protein complexes\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026gt;100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/8-sds-page-gel-preparation-kit?variant=52775642595509\"\u003e8% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eMedium to high molecular weight protein separation\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 50–200 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/10-sds-page-gel-preparation-kit?variant=52775673626805\"\u003e10% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eGeneral protein electrophoresis and routine Western blot workflows\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 20–150 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/12-sds-page-gel-preparation-kit?variant=52775704723637\"\u003e12% SDS-PAGE Gel\u003c\/a\u003e\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eRoutine protein separation with improved resolution for smaller proteins\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 10–100 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003e15% SDS-PAGE Gel\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSmall protein, peptide, and low molecular weight protein analysis\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u0026lt;50 kDa proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPREPARATION WORKFLOW\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe RP0261 \u003cstrong\u003eOne-Step SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e provides a simplified workflow for preparing high-resolution protein electrophoresis gels. The pre-optimized formulation reduces manual preparation steps while maintaining compatibility with standard Tris-glycine SDS-PAGE systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eMix equal volumes of Separation Gel Solution and Separation Gel Buffer to prepare the resolving gel mixture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant to the separation gel solution and gently mix to initiate polymerization while avoiding bubble formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTransfer the prepared resolving gel solution into the gel cassette.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix Concentrating Gel Solution and Concentrating Gel Buffer to prepare the stacking gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd Novel Coagulant and directly cast the concentrating gel above the separation gel layer without waiting for complete resolving gel polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eInsert the comb and allow approximately 15 minutes for gel polymerization before performing SDS-PAGE electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe simplified casting procedure reduces preparation complexity and improves reproducibility for laboratories performing high-resolution separation of small proteins and peptide samples.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe RP0261 kit is designed for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e. Separation and concentrating gel buffers contain SDS and are not intended for native PAGE applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe 15% polyacrylamide formulation provides smaller pore sizes than lower percentage SDS-PAGE gels, making it suitable for enhanced resolution of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe colored concentrating gel improves visualization of sample loading wells and supports accurate sample application during electrophoresis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-percentage SDS-PAGE gels may provide improved separation of small proteins but may require optimization of electrophoresis conditions depending on sample properties.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGel polymerization speed is temperature dependent. Lower temperatures may extend polymerization time, while suitable room temperature conditions support efficient gel formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor optimal electrophoresis performance, freshly prepared gels are recommended for use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nDOCUMENTS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_Manual.docx?v=1783473556\"\u003e \u003cstrong\u003eManual\u003c\/strong\u003e \u003c\/a\u003e \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/15_One-Step_SDS-PAGE_Color_Gel_Fast_Preparation_Kit_MSDS.pdf?v=1783474528\"\u003e \u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003ca href=\"#\"\u003e \u003c\/a\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a 15% SDS-PAGE Gel Preparation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eA 15% SDS-PAGE Gel Preparation Kit is designed for high-resolution separation of small proteins, protein fragments, and peptide samples. The RP0261 kit provides a high-percentage polyacrylamide gel formulation suitable for applications requiring improved resolution of low molecular weight proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy choose a 15% SDS-PAGE gel instead of a 12% gel?\u003c\/summary\u003e\n\u003cp\u003eA 15% SDS-PAGE gel contains a higher acrylamide concentration and smaller pores compared with a 12% gel. This provides improved separation capability for smaller proteins and peptide samples that may not be sufficiently resolved using lower percentage gels.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan RP0261 be used for Western blot analysis?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0261 can be used for Western blot workflows requiring separation of low molecular weight proteins before membrane transfer and antibody-based detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for a 15% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eThe RP0261 15% SDS-PAGE gel is recommended for small proteins, protein fragments, and peptide samples generally below approximately 50 kDa. Actual separation performance depends on electrophoresis conditions and sample characteristics.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes RP0261 require additional TEMED during gel preparation?\u003c\/summary\u003e\n\u003cp\u003eNo. RP0261 uses an optimized coagulant system that simplifies SDS-PAGE gel preparation without requiring additional TEMED addition during the casting process.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat are the advantages of a high-percentage SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eHigh-percentage SDS-PAGE gels provide smaller pore sizes, allowing improved separation resolution for lower molecular weight proteins and peptides compared with lower percentage polyacrylamide gels.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs RP0261 compatible with standard SDS-PAGE electrophoresis equipment?\u003c\/summary\u003e\n\u003cp\u003eYes. RP0261 is designed for standard Tris-glycine SDS-PAGE systems and can be used with conventional PAGE gel casting and electrophoresis equipment.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between RP0261 15% gel and RP0251 12% gel?\u003c\/summary\u003e\n\u003cp\u003eRP0251 12% SDS-PAGE gel provides routine protein separation with improved resolution for many commonly analyzed proteins, while RP0261 15% SDS-PAGE gel is optimized for smaller proteins and peptide samples requiring higher resolution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52775732707509,"sku":"RP0261","price":98.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/15_One-Step_PAGE_Color_Gel_Quick_Preparation_Kit.jpg?v=1783475951"},{"product_id":"6-15-sds-page-gel-preparation-kit","title":"6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit","description":"\u003c!-- =========================\nPRODUCT DESCRIPTION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTraditional SDS-PAGE gel preparation requires multiple manual steps, including preparation of acrylamide solutions, separate preparation of resolving and stacking gel buffers, adjustment of gel concentration, and addition of polymerization reagents. These procedures can increase preparation time and introduce variation between experiments, especially when laboratories need different gel percentages for proteins with different molecular weights.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit (AP0641)\u003c\/strong\u003e is a complete \u003cstrong\u003eSDS-PAGE gel preparation kit\u003c\/strong\u003e designed for preparing polyacrylamide gels with adjustable separation ranges. The kit provides the essential reagents required for SDS-PAGE gel casting, including \u003cstrong\u003e30% Acr-Bis (29:1) solution\u003c\/strong\u003e, pre-mixed resolving gel buffer, pre-mixed stacking gel buffer, and a novel polymerization enhancer.\u003c\/p\u003e\n\u003cp\u003eUnlike conventional SDS-PAGE gel preparation workflows that require additional TEMED addition, the AP0641 \u003cstrong\u003eTEMED-free SDS-PAGE gel preparation kit\u003c\/strong\u003e uses an optimized polymerization enhancer system to simplify gel casting procedures. Researchers only need to provide gel casting equipment and distilled water to prepare SDS-PAGE gels for routine protein electrophoresis applications.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003e6–15% gradient SDS-PAGE gel preparation system\u003c\/strong\u003e allows users to prepare different polyacrylamide gel concentrations according to protein molecular weight requirements. From high molecular weight protein separation using lower percentage gels to improved resolution of smaller proteins using higher percentage gels, AP0641 provides flexibility for different protein electrophoresis workflows.\u003c\/p\u003e\n\u003cp\u003eThe stacking gel contains a visible non-migrating dye, allowing researchers to easily identify sample wells during loading. This feature improves loading accuracy and simplifies handling during \u003cstrong\u003eSDS-PAGE protein electrophoresis\u003c\/strong\u003e workflows. The AP0641 kit is suitable for applications including \u003cstrong\u003eWestern blot gel preparation, protein molecular weight analysis, recombinant protein characterization, and polyacrylamide gel electrophoresis\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eThe AP0641 \u003cstrong\u003eSDS-PAGE gel casting kit\u003c\/strong\u003e is supplied for research use only and provides a convenient alternative to conventional manual PAGE gel preparation methods for molecular biology and biotechnology laboratories.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT INFORMATION\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eSKU\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eAP0641\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eProduct Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eSDS-PAGE Gel Preparation Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Type\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003ePolyacrylamide SDS-PAGE Gel\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Concentration Range\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e6–15%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eAcrylamide\/Bisacrylamide Ratio\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e29:1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eElectrophoresis System\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eTris-Glycine SDS-PAGE System\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Preparation Method\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eManual gel casting with pre-mixed reagents\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003ePolymerization Time\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eApproximately 15 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eGel Capacity\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e50 gels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResolving Gel Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4× buffer containing Tris (pH 8.8) and SDS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStacking Gel Buffer\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4× buffer containing Tris (pH 6.8) and SDS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage Condition\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4°C; Polymerization enhancer stored at −20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eShelf Life\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e18 months\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eResearch Use\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nKIT COMPONENTS\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable cellspacing=\"0\" cellpadding=\"8\" border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e30% Acr-Bis (29:1)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eResolving Gel Buffer (4×)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e70 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eStacking Gel Buffer (4×)\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e30 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e4°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003eNovel Polymerization Enhancer\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e5 mL\u003c\/td\u003e\n\u003ctd style=\"text-align: center; vertical-align: middle;\"\u003e−20°C, protected from light\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFEATURES\n========================= --\u003e\n\u003csection\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAdjustable 6–15% SDS-PAGE gel preparation:\u003c\/strong\u003e The AP0641 kit enables preparation of different polyacrylamide gel concentrations according to experimental requirements, supporting separation of proteins across a broad molecular weight range.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eComplete SDS-PAGE gel casting solution:\u003c\/strong\u003e The kit contains pre-mixed resolving gel buffer and stacking gel buffer with SDS, reducing the number of preparation steps required for conventional PAGE gel casting.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eTEMED-free SDS-PAGE workflow:\u003c\/strong\u003e The included polymerization enhancer eliminates the need for additional TEMED addition during gel preparation, simplifying laboratory operation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRapid polyacrylamide gel preparation:\u003c\/strong\u003e SDS-PAGE gels can be prepared within approximately 15 minutes, improving efficiency for routine protein electrophoresis workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eVisible dye stacking gel:\u003c\/strong\u003e The non-migrating dye in the stacking gel provides clear visualization of sample wells for more accurate protein loading.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConsistent protein separation:\u003c\/strong\u003e Pre-mixed components help reduce variability caused by repeated manual preparation of individual gel reagents.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuitable for multiple protein analysis workflows:\u003c\/strong\u003e The flexible gel concentration range supports routine SDS-PAGE, Western blot sample separation, and molecular weight analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nAPPLICATIONS\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003e6–15% SDS-PAGE Gradient Gel Quick-Preparation Kit (AP0641)\u003c\/strong\u003e is designed for laboratories requiring flexible \u003cstrong\u003epolyacrylamide gel preparation\u003c\/strong\u003e for protein separation and analysis. By allowing researchers to prepare different SDS-PAGE gel concentrations, the kit supports a wide range of protein electrophoresis applications from high molecular weight proteins to smaller protein fragments.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein electrophoresis:\u003c\/strong\u003e Suitable for routine SDS-PAGE protein separation workflows requiring reliable polyacrylamide gel preparation and consistent protein band migration.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWestern blot sample preparation:\u003c\/strong\u003e Provides SDS-PAGE gels for separating protein samples before transfer to membranes for antibody-based detection workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein molecular weight analysis:\u003c\/strong\u003e Different gel percentages allow researchers to optimize separation conditions according to protein molecular weight and sample characteristics.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eRecombinant protein analysis:\u003c\/strong\u003e Suitable for evaluating recombinant protein expression, purification results, and molecular weight differences during biotechnology research.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow molecular weight protein separation:\u003c\/strong\u003e Higher percentage SDS-PAGE gels prepared from AP0641 provide improved resolution for smaller proteins compared with lower percentage polyacrylamide gels.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh molecular weight protein analysis:\u003c\/strong\u003e Lower percentage gels provide larger pore structures suitable for separation of larger proteins and protein complexes.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProtein characterization studies:\u003c\/strong\u003e Applicable for laboratories performing protein quality evaluation, sample comparison, and biochemical analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eMolecular biology and biotechnology research:\u003c\/strong\u003e Provides a convenient SDS-PAGE gel casting solution for academic laboratories, biotechnology companies, and research institutions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nGEL SELECTION GUIDE\n========================= --\u003e\n\u003csection\u003e\u003c\/section\u003e\n\u003csection\u003e\n\u003ch2\u003ePreparation Workflow\u003c\/h2\u003e\n\u003cp\u003eThe \u003cstrong\u003eAP0641 SDS-PAGE Gel Preparation Kit\u003c\/strong\u003e provides a simplified workflow for preparing polyacrylamide gels using pre-mixed reagents. The optimized formulation reduces preparation complexity while maintaining compatibility with standard SDS-PAGE electrophoresis systems.\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003eSelect the required SDS-PAGE gel concentration according to the molecular weight of the target protein.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMix distilled water, 30% Acr-Bis (29:1), and the appropriate 4× resolving gel buffer to prepare the resolving gel solution.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAdd the polymerization enhancer and mix gently to initiate gel formation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCast the resolving gel into the gel cassette and allow polymerization.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePrepare the stacking gel using the 4× stacking gel buffer, Acr-Bis solution, distilled water, and polymerization enhancer.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe visible dye-containing stacking gel allows easy identification of sample wells before protein loading.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePerform SDS-PAGE electrophoresis using either rapid electrophoresis buffer conditions or conventional Tris-glycine-SDS running buffer conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThe AP0641 workflow enables laboratories to prepare customized SDS-PAGE gels while reducing manual reagent preparation compared with traditional hand-casting methods.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nTECHNICAL NOTES\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eTechnical Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe AP0641 kit is designed for \u003cstrong\u003edenaturing SDS-PAGE electrophoresis\u003c\/strong\u003e workflows. The resolving and stacking gel buffers contain SDS and are intended for protein separation under denaturing conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe kit provides a 30% Acr-Bis (29:1) solution, allowing users to prepare different gel percentages according to experimental requirements.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe stacking gel contains a non-migrating dye that improves visualization of loading wells during sample application.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAt lower temperatures, SDS precipitation may occur in gel buffers. Warm the solutions gently and mix thoroughly before use.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePolymerization time depends on environmental conditions. Lower room temperatures may require adjustment of polymerization enhancer usage according to experimental conditions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe product is supplied for \u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e and is not intended for diagnostic or therapeutic applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nFAQ\n========================= --\u003e\n\u003csection\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a SDS-PAGE Gel Preparation Kit used for?\u003c\/summary\u003e\n\u003cp\u003eA \u003cstrong\u003eSDS-PAGE Gel Preparation Kit\u003c\/strong\u003e is used to prepare polyacrylamide gels for protein electrophoresis. The AP0641 kit provides Acr-Bis solution, resolving gel buffer, stacking gel buffer, and polymerization enhancer for preparing SDS-PAGE gels with different concentrations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eDoes the AP0641 SDS-PAGE gel kit require TEMED?\u003c\/summary\u003e\n\u003cp\u003eNo. The AP0641 kit uses a novel polymerization enhancer system and does not require additional TEMED addition during SDS-PAGE gel preparation. This simplifies gel casting procedures and reduces exposure to TEMED-related odor.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat gel percentages can be prepared with the AP0641 kit?\u003c\/summary\u003e\n\u003cp\u003eThe AP0641 \u003cstrong\u003egradient SDS-PAGE gel preparation kit\u003c\/strong\u003e supports preparation of 6%, 8%, 10%, 12%, and 15% polyacrylamide gels. The selected gel concentration depends on the molecular weight range and required separation resolution.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat proteins are suitable for 6–15% SDS-PAGE gels?\u003c\/summary\u003e\n\u003cp\u003eThe AP0641 kit supports protein separation across a broad molecular weight range. Lower percentage gels are suitable for larger proteins, while higher percentage gels provide improved separation of smaller proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan AP0641 be used for Western blot workflows?\u003c\/summary\u003e\n\u003cp\u003eYes. AP0641 can be used to prepare SDS-PAGE gels for Western blot workflows where protein samples require separation before membrane transfer and antibody detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the advantage of a colored stacking gel?\u003c\/summary\u003e\n\u003cp\u003eThe colored stacking gel contains a non-migrating dye that makes sample loading wells easier to identify. This helps improve loading accuracy during SDS-PAGE sample preparation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between resolving gel and stacking gel buffer?\u003c\/summary\u003e\n\u003cp\u003eThe resolving gel buffer contains Tris at pH 8.8 and SDS and forms the main separation layer of the SDS-PAGE gel. The stacking gel buffer contains Tris at pH 6.8 and SDS and helps concentrate protein samples before separation.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eHow long does it take to prepare an SDS-PAGE gel using AP0641?\u003c\/summary\u003e\n\u003cp\u003eThe AP0641 SDS-PAGE gel preparation workflow can produce a gel in approximately 15 minutes, depending on temperature and experimental conditions.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a 6% and 15% SDS-PAGE gel?\u003c\/summary\u003e\n\u003cp\u003eA 6% SDS-PAGE gel has larger pores and is suitable for higher molecular weight proteins, while a 15% SDS-PAGE gel provides smaller pores and improved resolution for lower molecular weight proteins.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eIs AP0641 suitable for native PAGE?\u003c\/summary\u003e\n\u003cp\u003eNo. AP0641 is designed for SDS-PAGE workflows and contains SDS in the gel buffer system. It is not intended for native PAGE applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003c\/section\u003e\n\u003c!-- =========================\nPRODUCT SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c!-- =========================\nFAQ SCHEMA\n========================= --\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"AccuRef Scientific","offers":[{"title":"Default Title","offer_id":52784226369717,"sku":"AP0641","price":48.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/6-15_SDS-PAGE_Gel_Preparation_Kit_with_Acr-Bis_Solution_and_Buffers.jpg?v=1783671116"}],"url":"https:\/\/biofargo.com\/collections\/gel-preparation-kits.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}