{"title":"DNA \/ RNA Extraction \u0026 Analysis","description":"\u003ch1\u003e\u003cstrong\u003eDNA\/RNA Extraction \u0026amp; Analysis\u003c\/strong\u003e\u003c\/h1\u003e\n\u003chr style=\"border: none; border-top: 2px solid #ccc; margin: 20px 0;\"\u003e\n\u003cp\u003eBiofargo's DNA \u0026amp; RNA Extraction, Analysis, Quantitation, and Purification kits offer high-quality, reliable tools for streamlined nucleic acid workflows. Designed for precision and efficiency, these kits ensure optimal yield and purity from a wide range of sample types. Whether for routine lab use or advanced molecular research, Biofargo delivers consistent performance with user-friendly protocols.\u003c\/p\u003e\n\u003cp style=\"font: arial;\"\u003e\u003cspan style=\"color: rgb(5, 93, 169);\"\u003e\u003cstrong\u003e➜ To inquire about our local laboratory pricing, or for bulk inquiries, please email us at contact@biofargo.com\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp style=\"font: arial;\"\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003e\u003cstrong\u003e🚫 These products require laboratory authentication. These products are strictly for research use only.\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003chr style=\"border: none; border-top: 2px solid #ccc; margin: 20px 0;\"\u003e\n\u003ch2\u003eExplore Our Featured Collections\u003c\/h2\u003e","products":[{"product_id":"qbtesttm-x-green-ii-transparent-tubes-100t-uelandy-q2038s","title":"Quantitative Fluorescence Reagent-100T-Q2038S","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II dsDNA Quantitation Kit Plus is sensitive fluorescence detection method which can be used to quantify the dsDNA. In molecular biology, it is commonly used in the construction of cDNA library and the purification and application of subcloned DNA fragments, such as DNA quantification, product amplification and further detection of primers. The conventional DNA content detection method is to measure its absorbance at 260 nm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe main disadvantage of this method is that nucleotides, single-stranded nucleic acids and proteins have a great influence on the signal, and they are also interfered by for Research Use only contaminants in the nucleic acid preparation process.Unable to distinguish between DNA and RNA, and the sensitivity is low (5 μg\/mL dsDNAA260=0.1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II emits fluorescence only after binding dsDNA, and the fluorescence intensity is proportional to the DNA concentration. QbtestTM X-Green II dsDNA Quantification Kit Plus can detect dsDNA in the range of 10 pg\/μL-100 ng\/μL, and the linear relationship is good (R2\u0026gt;0.99). The kit can be used for Qubit and can replace imported similar products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePlease try to avoid light to slow down the quenching of fluorescence.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e1× QbtestTM X-Green II working solution is prepared when used to ensure the best results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C and protect from light, Long-term storage can be stored at -20ºC. Expiration date marked on the outer packing.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Q2038.pdf?v=1626785861\" target=\"_blank\"\u003eQ2038\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS_Q2038_Qbtest_X-Green_II_dsDNA_Quantitation_Kit_Plus.pdf.pdf?v=1760163949\"\u003eQ2038 MSDS\u003c\/a\u003e\u003c\/strong\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"Uelandy","offers":[{"title":"A Group 250 µL","offer_id":40550227574965,"sku":"Q2038S-A","price":127.0,"currency_code":"USD","in_stock":true},{"title":"B Group 50 mL","offer_id":40550227607733,"sku":"Q2038S-B","price":127.0,"currency_code":"USD","in_stock":true},{"title":"C Group 1 mL","offer_id":40550227640501,"sku":"Q2038S-C","price":127.0,"currency_code":"USD","in_stock":true},{"title":"D Group 1 mL","offer_id":40550227673269,"sku":"Q2038S-D","price":127.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/biofargo.jpg?v=1761012618"},{"product_id":"qbtesttm-x-green-ii-white-tubes-500t-uelandy-q2038l","title":"Quantitative Fluorescence Reagent-500T-Q2038L","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II dsDNA Quantitation Kit Plus is sensitive fluorescence detection method which can be used to quantify the dsDNA. In molecular biology, it is commonly used in the construction of cDNA library and the purification and application of subcloned DNA fragments, such as DNA quantification, product amplification and further detection of primers. The conventional DNA content detection method is to measure its absorbance at 260 nm.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe main disadvantage of this method is that nucleotides, single-stranded nucleic acids and proteins have a great influence on the signal, and they are also interfered by for Research Use only contaminants in the nucleic acid preparation process.Unable to distinguish between DNA and RNA, and the sensitivity is low (5 μg\/mL dsDNAA260=0.1).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eQbtestTM X-Green II emits fluorescence only after binding dsDNA, and the fluorescence intensity is proportional to the DNA concentration. QbtestTM X-Green II dsDNA Quantification Kit Plus can detect dsDNA in the range of 10 pg\/μL-100 ng\/μL, and the linear relationship is good (R2\u0026gt;0.99). The kit can be used for Qubit and can replace imported similar products.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eNotes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePlease try to avoid light to slow down the quenching of fluorescence.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e1× QbtestTM X-Green II working solution is prepared when used to ensure the best results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at 4°C and protect from light, Long-term storage can be stored at -20ºC. Expiration date marked on the outer packing.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Q2038.pdf?v=1626785861\" target=\"_blank\"\u003eQ2038\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS_Q2038_Qbtest_X-Green_II_dsDNA_Quantitation_Kit_Plus.pdf.pdf?v=1760163949\"\u003e\u003cstrong\u003eQ2038 MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Uelandy","offers":[{"title":"A Group 1.25 mL","offer_id":40550251331765,"sku":"Q2038L-A","price":412.0,"currency_code":"USD","in_stock":true},{"title":"B Group 250 mL","offer_id":40550251364533,"sku":"Q2038L-B","price":412.0,"currency_code":"USD","in_stock":true},{"title":"C Group 5 mL","offer_id":40550251397301,"sku":"Q2038L-C","price":412.0,"currency_code":"USD","in_stock":true},{"title":"D Group 5 mL","offer_id":40550251430069,"sku":"Q2038L-D","price":412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/biofargo.jpg?v=1761012618"},{"product_id":"100ml-cccdna-purification-kit-222501-100","title":"cccDNA Purification Kit (100)-220501","description":"\u003c!-- ============================================================\n cccDNA Purification Kit — Upgraded Product Description\n Biofargo | Cat# 220501 \/ 220502\n \n WHAT'S INCLUDED IN THIS FILE:\n 1. 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References (clean formatted)\n\n HOW TO USE IN SHOPIFY:\n - Go to product page → Edit → click \"\u003c\/\u003e\" HTML view\n - Replace ALL existing description HTML with this file\n - Save and preview\n - Shopify's \u003cstyle\u003e tags in descriptions may be stripped by some themes;\n if styles don't apply, move \u003cstyle\u003e blocks to your theme's CSS file.\n ============================================================ --\u003e\n\n\u003c!-- ── GLOBAL STYLES ─────────────────────────────────────── --\u003e\n\u003cstyle\u003e\n .pd * { box-sizing: border-box; }\n .pd { font-family: -apple-system, BlinkMacSystemFont, 'Segoe UI', Helvetica, Arial, sans-serif; color: #222; line-height: 1.7; max-width: 820px; margin: 0 auto; padding: 0 16px; }\n .pd h2 { font-size: 18px; font-weight: 700; color: #111; margin: 36px 0 14px; padding-bottom: 8px; border-bottom: 2px solid #f0f0f0; }\n .pd h3 { font-size: 15px; font-weight: 600; color: #111; margin: 0 0 6px; }\n .pd p { margin: 0 0 14px; font-size: 14.5px; 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font-weight: 700; }\n .pd .ks-dash { color: #d1d5db; }\n .pd .ks-rec-text { font-size: 12px; color: #777; }\n\n .pd .ks-note { font-size: 12px; color: #aaa; text-align: center; line-height: 1.7; margin-top: 10px; }\n .pd .ks-note a { color: #2563EB; }\n\n \/* ── STORAGE CARD ── *\/\n .pd .storage-card { display: inline-flex; align-items: center; gap: 12px; background: #F0F9FF; border: 1px solid #BAE6FD; border-radius: 10px; padding: 12px 18px; margin-bottom: 20px; }\n .pd .storage-card .st-icon { font-size: 24px; flex-shrink: 0; }\n .pd .storage-card .st-text { font-size: 13.5px; color: #0C4A6E; }\n .pd .storage-card .st-text b { font-weight: 700; }\n\n \/* ── REFERENCES ── *\/\n .pd .ref-list { list-style: none; padding: 0; margin: 0; }\n .pd .ref-list li { font-size: 12.5px; color: #666; padding: 7px 0; border-bottom: 1px solid #f3f4f6; line-height: 1.55; }\n .pd .ref-list li:last-child { border-bottom: none; }\n .pd .ref-list li .ref-journal { font-style: italic; color: #2563EB; }\n\u003c\/style\u003e\n\n\u003c!-- ══════════════════════════════════════════════════════════ --\u003e\n\u003cdiv class=\"pd\"\u003e\n\n \u003c!-- ── 1. SEO-OPTIMIZED INTRO ─────────────────────────────── --\u003e\n \u003c!-- Target keywords: cccDNA purification kit, enzyme-free cccDNA isolation,\n HBV cccDNA extraction, eccDNA purification, extrachromosomal circular DNA,\n cccDNA from plasma, circulating eccDNA biomarker --\u003e\n \u003cp class=\"seo-intro\"\u003e\n The \u003cstrong\u003eBiofargo cccDNA Purification Kit\u003c\/strong\u003e provides fast, \u003cstrong\u003eenzyme-free isolation of covalently closed circular DNA (cccDNA)\u003c\/strong\u003e from total DNA samples — no Exonuclease III, Plasmid-Safe DNase, or T5 Exonuclease required. Designed for \u003cstrong\u003eHBV cccDNA research\u003c\/strong\u003e, \u003cstrong\u003ecirculating eccDNA profiling\u003c\/strong\u003e, and \u003cstrong\u003ecancer biomarker discovery\u003c\/strong\u003e from plasma, liver tissue, and cell models. Compatible with silica columns and magnetic bead workflows. 100 preps per kit. \u003cem\u003eFor research use only.\u003c\/em\u003e\n \u003c\/p\u003e\n\n \u003c!-- ── 2. DESCRIPTION ─────────────────────────────────────── --\u003e\n \u003ch2\u003eDescription\u003c\/h2\u003e\n \u003cp\u003e\n Covalently closed circular DNA (cccDNA) is the natural topological form of most plasmids and a key replicative intermediate in viruses such as \u003cstrong\u003ehepatitis B virus (HBV)\u003c\/strong\u003e. In eukaryotes, cccDNA-like structures — known as \u003cstrong\u003eextrachromosomal circular DNA (eccDNA)\u003c\/strong\u003e — play roles in innate immune signaling, cell-to-cell communication, genetic heterogeneity, gene dosage compensation, aging, and gene regulation. Cell-free eccDNAs in bodily fluids are emerging as promising \u003cstrong\u003enon-invasive biomarkers\u003c\/strong\u003e for cancer diagnosis and prognosis.\n \u003c\/p\u003e\n \u003cp\u003e\n Traditional cccDNA isolation relies on multiple rounds of enzymatic digestion (Exonuclease III, Plasmid-Safe DNase, Exonuclease I\/III combinations, or T5 Exonuclease) to deplete linear and open-circular DNA, introducing risk of residual nuclease damage and protocol variability. This kit uses a \u003cstrong\u003eproprietary, enzyme-free extraction chemistry\u003c\/strong\u003e that exploits the topological differences between circular and non-circular DNA — delivering high-purity cccDNA in a single streamlined step.\n \u003c\/p\u003e\n\n \u003c!-- ── 3. FEATURES ────────────────────────────────────────── --\u003e\n \u003ch2\u003eKey features\u003c\/h2\u003e\n \u003cdiv class=\"feat-grid\"\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e01\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003eNo enzymatic digestion\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eEliminates residual nuclease risk that can nick or degrade purified cccDNA\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e02\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003eOne-step protocol\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eStreamlined workflow significantly reduces hands-on time vs. multi-enzyme methods\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e03\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003eHigh recovery efficiency\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eMinimal contamination with linear or open-circular DNA forms\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e04\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003eAll cccDNA sizes\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eBroad compatibility — from small plasmid-sized cccDNA to large eccDNA species\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e05\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003eFlexible format\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eWorks with silica-based spin columns and magnetic bead systems\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"feat-card\"\u003e\n \u003cdiv class=\"feat-num\"\u003e06\u003c\/div\u003e\n \u003cdiv class=\"feat-title\"\u003e100 preps per kit\u003c\/div\u003e\n \u003cdiv class=\"feat-desc\"\u003eSufficient for method development through moderate-throughput studies\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- ── 4. APPLICATIONS ───────────────────────────────────── --\u003e\n \u003ch2\u003eApplications\u003c\/h2\u003e\n \u003cdiv class=\"app-grid\"\u003e\n \u003cdiv class=\"app-card\"\u003e\n \u003cspan class=\"app-tag\"\u003eVirology\u003c\/span\u003e\n \u003ch3\u003eHBV cccDNA research\u003c\/h3\u003e\n \u003cp\u003eIsolation of HBV cccDNA from infected hepatocytes, liver tissue biopsies, and in vitro cell models for antiviral drug studies\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"app-card\"\u003e\n \u003cspan class=\"app-tag\"\u003eOncology\u003c\/span\u003e\n \u003ch3\u003eCancer biomarker discovery\u003c\/h3\u003e\n \u003cp\u003eExtraction of circulating eccDNA from plasma and serum for liquid biopsy, diagnostic, and prognostic research\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"app-card\"\u003e\n \u003cspan class=\"app-tag\"\u003eAging \u0026amp; Genetics\u003c\/span\u003e\n \u003ch3\u003eeccDNA profiling\u003c\/h3\u003e\n \u003cp\u003eProfiling eccDNA landscapes in aging models, cancer cell lines, and tissues to study heterogeneity and gene dosage\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"app-card\"\u003e\n \u003cspan class=\"app-tag\"\u003eGenomics\u003c\/span\u003e\n \u003ch3\u003eGene expression \u0026amp; sponging\u003c\/h3\u003e\n \u003cp\u003eResearch into eccDNA-mediated regulation of gene expression and miRNA sponging mechanisms\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"app-card\"\u003e\n \u003cspan class=\"app-tag\"\u003eHigh-throughput\u003c\/span\u003e\n \u003ch3\u003eDNA structure analysis\u003c\/h3\u003e\n \u003cp\u003eLarge-scale cccDNA profiling by NGS or qPCR with reproducible, enzyme-free sample prep\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- ── 5. KIT SELECTOR ───────────────────────────────────── --\u003e\n \u003ch2\u003eChoose your kit — 220501 vs 220502\u003c\/h2\u003e\n \u003cp style=\"font-size:13.5px;color:#666;margin-bottom:16px;\"\u003eBoth kits use the same enzyme-free workflow. The difference is what's included in the box.\u003c\/p\u003e\n\n \u003cdiv class=\"ks-cards\"\u003e\n\n \u003c!-- 220501 --\u003e\n \u003cdiv class=\"ks-card\" id=\"pd-card-501\" onclick=\"pdSelect('501')\"\u003e\n \u003cspan class=\"ks-badge ks-badge-blue\"\u003eCat# 220501\u003c\/span\u003e\n \u003cp class=\"ks-name\"\u003eStandard Kit\u003c\/p\u003e\n \u003cp class=\"ks-price\"\u003e$455\u003c\/p\u003e\n \u003cp class=\"ks-price-sub\"\u003e$4.55 per prep  ·  100 preps\u003c\/p\u003e\n \u003chr class=\"ks-divider\"\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-blue\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003e\u003cb\u003eExtraction Solution A\u003c\/b\u003e — 100 mL included\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-gray\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003eExtraction Solution B \u003cspan class=\"ks-pill pill-diy\"\u003eprepare in-house\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-gray\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003ePrecipitation Solution \u003cspan class=\"ks-pill pill-diy\"\u003eprepare in-house\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-blue\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003eEnzyme-free, one-step protocol\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-blue\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003eColumn \u0026amp; magnetic bead compatible\u003c\/span\u003e\n\u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c!-- 220502 --\u003e\n \u003cdiv class=\"ks-card sel-502\" id=\"pd-card-502\" onclick=\"pdSelect('502')\"\u003e\n \u003cdiv style=\"display:flex;align-items:center;flex-wrap:wrap;gap:5px;margin-bottom:10px;\"\u003e\n \u003cspan class=\"ks-badge ks-badge-teal\" style=\"margin-bottom:0\"\u003eCat# 220502\u003c\/span\u003e\n \u003cspan class=\"ks-badge-rec\"\u003e✓ Recommended\u003c\/span\u003e\n \u003c\/div\u003e\n \u003cp class=\"ks-name\"\u003eExtended Kit\u003c\/p\u003e\n \u003cp class=\"ks-price\"\u003e$640\u003c\/p\u003e\n \u003cp class=\"ks-price-sub\"\u003e$6.40 per prep  ·  100 preps\u003c\/p\u003e\n \u003chr class=\"ks-divider\"\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-teal\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003e\u003cb\u003eExtraction Solution A\u003c\/b\u003e \u003cspan class=\"ks-pill pill-inc\"\u003eincluded\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-teal\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003e\u003cb\u003eExtraction Solution B\u003c\/b\u003e \u003cspan class=\"ks-pill pill-inc\"\u003eincluded\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-teal\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003e\u003cb\u003ePrecipitation Solution\u003c\/b\u003e \u003cspan class=\"ks-pill pill-inc\"\u003eincluded\u003c\/span\u003e\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-teal\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003eEnzyme-free, one-step protocol\u003c\/span\u003e\n\u003c\/div\u003e\n \u003cdiv class=\"ks-row\"\u003e\n\u003cspan class=\"ks-dot dot-teal\"\u003e\u003c\/span\u003e\u003cspan class=\"ks-row-text\"\u003eNo additional reagent preparation needed\u003c\/span\u003e\n\u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003c\/div\u003e\n\n \u003c!-- Compare table --\u003e\n \u003cp class=\"ks-section-label\"\u003eFull kit contents comparison\u003c\/p\u003e\n \u003cdiv class=\"ks-table-wrap\"\u003e\n \u003ctable class=\"ks-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth style=\"width:36%\"\u003eComponent \/ feature\u003c\/th\u003e\n \u003cth class=\"col-501\"\u003e220501 Standard\u003c\/th\u003e\n \u003cth class=\"col-502\"\u003e220502 Extended\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003ecccDNA Extraction Solution A\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e 100 mL\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e 100 mL\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003ecccDNA Extraction Solution B\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-dash\"\u003e—\u003c\/span\u003e prepare in-house\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e included\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003ecccDNA Precipitation Solution\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-dash\"\u003e—\u003c\/span\u003e prepare in-house\u003c\/td\u003e\n \u003ctd\u003e\n\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e included\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003ePreps per kit\u003c\/td\u003e\n \u003ctd\u003e100\u003c\/td\u003e\n \u003ctd\u003e100\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003eEnzyme-free workflow\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003eSilica column compatible\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003eMagnetic bead compatible\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003ctd\u003e\u003cspan class=\"ks-check\"\u003e✓\u003c\/span\u003e\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003eStorage\u003c\/td\u003e\n \u003ctd\u003e4 °C  ·  ≥1 year\u003c\/td\u003e\n \u003ctd\u003e4 °C  ·  ≥1 year\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd class=\"feat\"\u003eBest for\u003c\/td\u003e\n \u003ctd class=\"ks-rec-text\"\u003eLabs that can prepare Solution B \u0026amp; Precipitation Solution in-house\u003c\/td\u003e\n \u003ctd class=\"ks-rec-text\"\u003eLabs wanting a fully ready-to-use, self-contained kit — no extra reagent prep\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n\n \u003cp class=\"ks-note\"\u003eNot sure which kit fits your lab? Email \u003ca href=\"mailto:contact@biofargo.com\"\u003econtact@biofargo.com\u003c\/a\u003e or call \u003ca href=\"tel:+18045292296\"\u003e(804) 529-2296\u003c\/a\u003e\u003c\/p\u003e\n\n \u003c!-- ── 6. SAMPLE REQUIREMENTS (warning box) ──────────────── --\u003e\n \u003ch2\u003eInput sample requirements\u003c\/h2\u003e\n \u003cdiv class=\"warn-box\"\u003e\n \u003cdiv class=\"warn-title\"\u003e⚠ Important — read before starting\u003c\/div\u003e\n \u003cul\u003e\n \u003cli\u003e\n\u003cb\u003eRecommended extraction method:\u003c\/b\u003e SDS-proteinase K digestion followed by alcohol precipitation. Proteinase K releases cccDNA from protein covalent linkages; alcohol precipitation recovers both cccDNA and non-cccDNA non-exclusively.\u003c\/li\u003e\n \u003cli\u003e\n\u003cb\u003eProcess immediately or freeze:\u003c\/b\u003e To prevent nicking by residual DNase, begin cccDNA purification immediately after total DNA isolation — or quick-freeze the total DNA preparation and store at \u003cb\u003e−80 °C\u003c\/b\u003e until ready.\u003c\/li\u003e\n \u003cli\u003e\n\u003cb\u003eSolvent:\u003c\/b\u003e Total DNA must be dissolved in \u003cb\u003eDNase-free water or TE buffer\u003c\/b\u003e. Other solvents may be incompatible — a trial experiment is required before use.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n\n \u003c!-- ── 7. STORAGE ─────────────────────────────────────────── --\u003e\n \u003ch2\u003eStorage and handling\u003c\/h2\u003e\n \u003cdiv class=\"storage-card\"\u003e\n \u003cdiv class=\"st-text\"\u003eStore at \u003cb\u003e4 °C\u003c\/b\u003e  ·  Stable for \u003cb\u003eat least 1 year\u003c\/b\u003e from date of receipt\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/cccDNA_Purification_Kit_Current_Manual.pdf?v=1770754088\" target=\"_blank\"\u003e\u003cstrong\u003e📄 Download user manual (PDF)\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\n \u003c!-- ── 8. REFERENCES ─────────────────────────────────────── --\u003e\n \u003ch2\u003eReferences\u003c\/h2\u003e\n \u003col class=\"ref-list\"\u003e\n \u003cli\u003eWang Y, et al. Purification, full-length sequencing and genomic origin mapping of eccDNA. \u003cspan class=\"ref-journal\"\u003eNature Protocols\u003c\/span\u003e, 2022.\u003c\/li\u003e\n \u003cli\u003eWang Y, et al. eccDNAs are apoptotic products with high innate immunostimulatory activity. \u003cspan class=\"ref-journal\"\u003eNature\u003c\/span\u003e, 2021.\u003c\/li\u003e\n \u003cli\u003eWang M, et al. Extrachromosomal Circular DNAs: Origin, formation and emerging function in Cancer. \u003cspan class=\"ref-journal\"\u003eInt J Biol Sci\u003c\/span\u003e 17(4): 1010–1025, 2021.\u003c\/li\u003e\n \u003cli\u003eHirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. \u003cspan class=\"ref-journal\"\u003eJ Mol Biol\u003c\/span\u003e 26:365–369, 1967.\u003c\/li\u003e\n \u003cli\u003evan Loon N, Miller D, Murnane JP. Formation of extrachromosomal circular DNA in HeLa cells by nonhomologous recombination. \u003cspan class=\"ref-journal\"\u003eNucleic Acids Res\u003c\/span\u003e 22(13):2447–2452, 1994.\u003c\/li\u003e\n \u003cli\u003eGaubatz JW. Purification of eucaryotic extrachromosomal circular DNAs using exonuclease III. \u003cspan class=\"ref-journal\"\u003eAnal Biochem\u003c\/span\u003e 184(2):305–310, 1990.\u003c\/li\u003e\n \u003cli\u003eWerle-Lapostolle B, et al. Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy. \u003cspan class=\"ref-journal\"\u003eGastroenterology\u003c\/span\u003e 126:1750–1758, 2004.\u003c\/li\u003e\n \u003cli\u003eKock J, et al. Generation of covalently closed circular DNA of hepatitis B viruses via intracellular recycling is regulated in a virus specific manner. \u003cspan class=\"ref-journal\"\u003ePLoS Pathog\u003c\/span\u003e 6:e1001082, 2010.\u003c\/li\u003e\n \u003cli\u003eSchnepp BC, et al. Genetic fate of recombinant adeno-associated virus vector genomes in muscle. \u003cspan class=\"ref-journal\"\u003eJ Virol\u003c\/span\u003e 77:3495–3504, 2003.\u003c\/li\u003e\n \u003cli\u003eLuo J, et al. Identification of an intermediate in HBV covalently closed circular (ccc) DNA formation and sensitive and selective cccDNA detection. \u003cspan class=\"ref-journal\"\u003eJ Virol\u003c\/span\u003e 91:e00539-17, 2017.\u003c\/li\u003e\n \u003cli\u003eQu B, et al. T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of cccDNA by PCR. \u003cspan class=\"ref-journal\"\u003eJ Virol\u003c\/span\u003e 92(23):e01117-18, 2018.\u003c\/li\u003e\n \u003cli\u003eVinograd J, Lebowitz J. Physical and Topological Properties of Circular DNA. \u003cspan class=\"ref-journal\"\u003eJ Gen Physiol\u003c\/span\u003e 49(6P2):103, 1966.\u003c\/li\u003e\n \u003cli\u003eShibata Y, Kumar P, et al. Extrachromosomal MicroDNAs and Chromosomal Microdeletions in Normal Tissues. \u003cspan class=\"ref-journal\"\u003eScience\u003c\/span\u003e 336(6077):82–86, 2012.\u003c\/li\u003e\n \u003c\/ol\u003e\n\n\u003c\/div\u003e\n\u003c!-- end .pd --\u003e\n\n\u003c!-- ── INTERACTIVE KIT SELECTOR SCRIPT ───────────────────── --\u003e\n\u003cscript\u003e\nfunction pdSelect(cat) {\n var c501 = document.getElementById('pd-card-501');\n var c502 = document.getElementById('pd-card-502');\n if (!c501 || !c502) return;\n c501.classList.remove('sel-501','sel-502');\n c502.classList.remove('sel-501','sel-502');\n if (cat === '501') { c501.classList.add('sel-501'); }\n else { c502.classList.add('sel-502'); }\n}\n\u003c\/script\u003e","brand":"oriscience","offers":[{"title":"100 Preps","offer_id":52071856636085,"sku":"220501","price":455.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Snipaste_2024-04-11_15-35-22.png?v=1732867618"},{"product_id":"100ml-cccdna-purification-kit-222502-100-with-cccdna-extraction-solution-b-cccdna-precipitation-solution","title":"cccDNA Purification Kit (100)-220502","description":"\u003cp\u003eFast, non-enzymatic isolation of covalently closed circular DNA (cccDNA) from total DNA samples. Ideal for HBV studies, circulating eccDNA profiling, and cancer biomarker research. 100 preps\/kit. For research use only.\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eCovalently closed circular DNA (cccDNA) is the natural form of most plasmids. It is also a key replicative intermediate in the life cycles of certain viruses, such as hepatitis B virus (HBV). In higher organisms, including humans, cccDNA-like structures are referred to as \u003cstrong\u003eextrachromosomal circular DNA (eccDNA)\u003c\/strong\u003e. These molecules have been shown to play roles in eliciting innate immune responses, mediating cell-to-cell communication, promoting genetic heterogeneity, compensating for genetic loss, contributing to aging, regulating gene expression, and functioning as molecular sponges. Additionally, cell-free eccDNAs circulating in bodily fluids have emerged as potential biomarkers for the diagnosis and prognosis of various cancers.\u003c\/p\u003e\n\u003cp\u003eThe isolation of cccDNA from non-cccDNA is critical for studying its biological functions. Various isolation methods have been reported. Several techniques exploit the structural differences between chromosomal and circular DNA, enabling their separation via \u003cstrong\u003eSDS lysis–salt precipitation\u003c\/strong\u003e or \u003cstrong\u003ehigh-speed ultracentrifugation in cesium chloride gradients\u003c\/strong\u003e.\u003c\/p\u003e\n\u003cp\u003eMost protocols involve total DNA extraction followed by multiple rounds of enzymatic digestion to eliminate non-cccDNA, and a final step of cccDNA purification. Enzymes used include:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eExonuclease III\u003c\/strong\u003e, which digests linear and open circular forms of DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePlasmid-Safe DNase\u003c\/strong\u003e, which preferentially hydrolyzes double-stranded linear DNA (dslDNA), and to a lesser extent, linear and closed circular single-stranded DNA (ssDNA)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eA combination of \u003cstrong\u003eExonuclease I and Exonuclease III\u003c\/strong\u003e, which degrade DNA strands with free 3′ ends\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eT5 Exonuclease\u003c\/strong\u003e, which specifically digests nicked double-stranded circular DNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis product is designed to purify cccDNA from either total DNA samples or partially purified preparations, offering a streamlined, enzyme-free workflow.\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eNo Enzymatic Digestion Required \u003c\/strong\u003e— avoids DNA degradation from residual nucleases\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOne-Step Extraction Protocol \u003c\/strong\u003e— time-saving and user-friendly\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh cccDNA Recovery Efficiency \u003c\/strong\u003e— minimal contamination with non-circular forms\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad Compatibility \u003c\/strong\u003e— suitable for cccDNA of all sizes\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFlexible Format \u003c\/strong\u003e— compatible with silica-based columns and magnetic bead systems\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003e100 Preparations per Kit\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFor Research Use Only\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHBV Research: Isolation of HBV cccDNA from infected liver tissues or cell models\u003c\/strong\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCancer Biomarker Discovery: Extraction of circulating eccDNA from plasma for diagnostic\/prognostic research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eAging and Genetic Heterogeneity Studies: eccDNA profiling in aging models and cancer cell lines\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGene Expression and Molecular Sponging Research\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh-throughput DNA Structure Analysis\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKit Contents\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eCAT#:220502\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003ecccDNA Extraction Solution A\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003ecccDNA Extraction Solution B*\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003ecccDNA Precipitation Solution*\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong data-start=\"104\" data-end=\"113\"\u003eNote:\u003c\/strong\u003e For greater convenience, \u003cstrong data-start=\"139\" data-end=\"171\"\u003ecccDNA Extraction Solution B\u003c\/strong\u003e and \u003cstrong data-start=\"176\" data-end=\"209\"\u003ecccDNA Precipitation Solution\u003c\/strong\u003e are included in the extended version (\u003cstrong data-start=\"248\" data-end=\"263\"\u003eCAT# 220502\u003c\/strong\u003e), eliminating the need to prepare additional reagents separately.\u003c\/p\u003e\n\u003cdiv title=\"Page 1\" class=\"page\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003ch2\u003e\u003cspan\u003eInput Sample Requirements\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv title=\"Page 1\" class=\"page\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eHighly suggest to use SDS-proteinase K digestion and alcohol \u003c\/span\u003e\u003cspan\u003eprecipitation method to isolated total DNA since proteinase K can release cccDNA from possible covalent link to proteins. In addition, alcohol precipitation can recover non-exclusively both non-cccDNA and cccDNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eTo avoid the nicking of cccDNA by residual DNase in total DNA preparation, perform cccDNA purification immediately after total DNA isolation, or quick-freeze total DNA preparation immediately \u003c\/span\u003e\u003cspan\u003eand store at \u003c\/span\u003e\u003cspan\u003e–\u003c\/span\u003e\u003cspan\u003e80\u003c\/span\u003e\u003cspan\u003e°C \u003c\/span\u003e\u003cspan\u003euntil cccDNA isolation.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTotal DNA should be dissolved in DNase-free water or TE solution, not in other solvent, otherwise a trial experiment is needed to test the compatibility of this solvent and this kit.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage and Handling \u003c\/h2\u003e\nStore the kit at 4\u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e°C\u003c\/span\u003e. The kit is stable for at least one year from date of receipt.\u003c\/div\u003e\n\u003cp class=\"column\"\u003e \u003c\/p\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cstrong\u003eUser Manual:\u003c\/strong\u003e\n\u003cp style=\"font-weight: 400;\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/220501_220502_cccDNA_Purification_Kit_Manual_v1.0_-English_1.pdf?v=1649882166\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eClick here for more guides and user manual.\u003c\/strong\u003e\u003cbr\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\n\u003ch2 style=\"font-weight: 400;\"\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eYuangao Wang, et al. Purification, full-length sequencing and gemonic origin mapping of eccDNA, 2022 Nature Protocals\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cp\u003eYuangao Wang, et al. eccDNAs are apoptotic products with high innate immunostimulatory activity. \u003cmeta charset=\"utf-8\"\u003e2021 Nature\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"column\"\u003eMan Wang, et al. 2021. Extrachromosomal Circular DNAs: Origin, formation and emerging function in Cancer. International Journal of Biological Sciences 17(4): 1010-1025\u003cbr\u003e\u003cbr\u003eHirt B. 1967. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol 26:365–369.\u003cbr\u003e\u003cbr\u003evan Loon N, Miller D, Murnane JP. 1994. Formation of extrachromosomal circular DNA in HeLa cells by nonhomologous recombination. Nucleic Acids Research:22(13):2447–2452. \u003cbr\u003e\u003cbr\u003eJames W. Gaubatz, 1990. Purification of eucaryotic extrachromosomal circular DNAs using exonuclease III. Analytical Biochemistry 184(2) : 305-310\u003cbr\u003e\u003cbr\u003eWerle-Lapostolle B, et.al. 2004. Persistence of cccDNA during the natural history of chronic hepatitis B and decline during adefovir dipivoxil therapy. Gastroenterology 126:1750–1758\u003cbr\u003e\u003cbr\u003eKock J, et al. 2010. Generation of covalently closed circular DNA of hepatitis B viruses via intracellular recycling is regulated in a virus specific manner. PLoS Pathog. 6:e1001082\u003cbr\u003e\u003cbr\u003eSchnepp BC, et al. 2003. Genetic fate of recombinant adeno-associated virus vector genomes in muscle.  J. Virol. 77:3495–3504\u003cbr\u003e\u003cbr\u003eLuo J, Cui X, Gao L, Hu J. 2017. Identification of an intermediate in hepatitis B virus covalently closed circular (ccc) DNA formation and sensitive and selective cccDNA detection. J. Virol. 91:e00539-17).\u003cbr\u003e\u003cbr\u003eBingqian Qu,et al. 2018. T5 Exonuclease Hydrolysis of Hepatitis B Virus Replicative Intermediates Allows Reliable Quantification and Fast Drug Efficacy Testing of Covalently Closed Circular DNA by PCR. Virol.92(23) :e01117-18\u003cbr\u003e\u003cbr\u003eVinograd J, Lebowitz J. 1966. Physical and Topological Properties of Circular DNA. Journal of General Physiology. 49(6P2):103. \u003cbr\u003e\u003cbr\u003eShibata Y, Kumar P, et al. 2012. Extrachromosomal MicroDNAs and Chromosomal Microdeletions in Normal Tissues. Science. 336(6077): 82–86.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"page\" title=\"Page 1\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003cdiv class=\"column\"\u003e\n\u003cdiv class=\"page\" title=\"Page 1\"\u003e\n\u003cdiv class=\"section\"\u003e\n\u003cdiv class=\"layoutArea\"\u003e\n\u003col\u003e\u003c\/ol\u003e\n\u003cbr\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"oriscience","offers":[{"title":"100 Preps","offer_id":52071856013493,"sku":"220502","price":640.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/cccDNA_Kits_2.png?v=1732867620"},{"product_id":"animal-tissue-cell-total-rna-extraction-kit","title":"Animal Tissue Cell Total RNA Extraction Kit-Biosci™","description":"\u003ch2\u003eProduct Description:\u003c\/h2\u003e\n\u003cp\u003eAnimal Tissue Cell Total RNA Extraction Kit employs a rapid, phenol-free and chloroform-free RNA extraction technique for swift extraction of total RNA from easily lysable animal tissue and cell. The lysis buffer in this kit rapidly lyses cell and inactivates RNase, without the need for β-mercaptoethanol from conventional kits. The unique genomic DNA removal column guarantees effective removal of gDNA residue, eliminating the need for DNase digestion and allowing direct use of RNA for tests such as RT-PCR and qPCR. If downstream experiments are highly sensitive to trace DNA contamination, additional DNase treatment can be employed.\u003c\/p\u003e\n\u003cp\u003eThis kit extracts high-purity RNA fast and easily. Generally, the operation of a single sample can be completed within 30 minutes.\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\" style=\"margin-bottom: 16px; float: none;\" data-mce-style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115163041_2048x2048.png?v=1700037064\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\" data-mce-style=\"text-align: start;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003eStorage and Transportation:\u003c\/h2\u003e\n\u003cp\u003eStore at 2°C - 30°C, with a shelf life of 12 months.\u003cbr\u003eTransport at room temperature.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eScope ofApplication:\u003c\/h2\u003e\n\u003cp\u003eThe product can only be used for in vitro research\u003cbr\u003eand not for diagnostic purposes.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Cultured Animal Cell Total RNA: (please read the precautions before the test).\u003c\/h2\u003e\n\u003cp\u003e1. Collect ≤ 1 × 10 7 cells by centrifuging at 300 × g for 5 min or 13,000 × g for 10 s, and remove supernatant.\u003c\/p\u003e\n\u003cp\u003e2. Add 350 μL (\u0026lt; 5 × 10 6 cells) or 600 μL (5 × 10 6 - 1 × 10 7 cells) of RLT lysis buffer, mix well by pipetting up and down, shake vigorously by hand for 20 s, and add all the lysis mixture to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Centrifuge at 13,000 × g for 1 min and retain the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Ensure that all liquid is filtered after centrifugation. Increase the centrifugal force and time if necessary.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e4. Accurately measure the volume of the filtrate with a pipette, add an equal volume of 70% ethanol and mix well by pipetting up and down.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e5. Immediately transfer the mixture to the adsorption column, which is put into the collection tube, centrifuge at 13,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Do not transfer more than 700 μL per time. If more than 700 μL of mixture needs to be transferred, do it in two separate transfers and centrifuge each time.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e6. Add 700 μL of rinsing solution 1, place at room temperature for 1 min, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e7. Add 500 μL of rinsing solution 2, centrifuge at 12,000 × g for 30 s, and discard the filtrate.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: Check whether absolute ethanol has been added to the reagent bottle.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e8. Repeat Step 7.\u003c\/p\u003e\n\u003cp\u003e9. Put the adsorption column back into the collection tube and centrifuge at 13,000 × g for 2 minutes to completely remove residual ethanol via spin-drying.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The presence of residual ethanol can inhibit downstream reactions.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e10. Put the adsorption column into an RNase-free 1.5 mL centrifuge tube, add 30 μL - 50 μL of eluent to the center of the adsorption column membrane, allow it to stand for 1 min, and centrifuge at 12,000 × g for 1min.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote: The yield can be improved by preheating the eluent in a water bath at 50°C - 70°C. If the expected RNA yield exceeds 30 μg, suck the eluent back into the adsorption column and repeat Step 10; or add another 30 μL - 50 μL of eluent and repeat Step 10, and mix the two eluents.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eExtraction Steps of Animal Tissue Total RNA: (please read the precautions before the test)\u003c\/h2\u003e\n\u003cp\u003e1. Samples are first electrically homogenized or ground with liquid nitrogen.\u003c\/p\u003e\n\u003cp\u003e     a. \u003cstrong\u003eElectric homogenization:\u003c\/strong\u003e Quickly cut fresh tissues into small pieces with a scalpel, add 350 μL (\u0026lt; 20 mg of tissue) or 600 μL (20 mg - 30 mg of tissue) of RLT lysis buffer, and then electrically homogenize the mixture thoroughly for 20 - 40 s.\u003c\/p\u003e\n\u003cp\u003e     b. \u003cstrong\u003eGrinding with liquid nitrogen:\u003c\/strong\u003e Grind the tissue into fine powder with      liquid nitrogen, transfer an appropriate amount of fine tissue powder (20 mg\/30 mg) into an RNase-free 1.5 mL centrifuge tube containing 350 μL\/600 μL of RLT lysis buffer, and shake the tube vigorously by hand for 20 s to fully lyse.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eOptional steps:\u003c\/strong\u003e Use a disposable 1 mL (with a 0.9 mm needle) syringe with a blunt needle to vigorously push and pull the plunger at least 10 times until a satisfactory homogenization effect is obtained, so as to shear DNAand reduce viscosity to prevent column plugging and improve yield.\u003c\/p\u003e\n\u003cp\u003e2. Centrifuge the homogenized mixture at 13,000 × g for 3 min, and transfer all the supernatant to the removal column, which is put into the collection tube.\u003c\/p\u003e\n\u003cp\u003e3. Follow Step 3 of extraction of cultured animal cell total RNA.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperation Steps:\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003cimg src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" style=\"margin-bottom: 16px; float: none;\" data-mce-src=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/20231115164153_1024x1024.png?v=1700037730\" data-mce-style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: center;\" data-mce-style=\"text-align: center;\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003c\/h2\u003e\n\u003ch2\u003ePrecautions:\u003c\/h2\u003e\n\u003cp\u003e1. Please read the operating instructions carefully before use.\u003c\/p\u003e\n\u003cp\u003e2. Before the first use, add absolute ethanol to the rinsing solution 2 reagent bottle (formal pack: 42 mL; trial pack: 4.2 mL); add absolute ethanol to the 70% ethanol bottle (formal pack: 21 mL; trial pack: 2.1 mL).\u003c\/p\u003e\n\u003cp\u003e3. Ensure that all solutions are clear before use. Low-temperature storage can cause precipitation and affect the use effect. The solution can be restored to clarity in a water bath at 37°C for several minutes.\u003c\/p\u003e\n\u003cp\u003e4. Perform all centrifugation at room temperature.\u003c\/p\u003e\n\u003cp\u003e5. The sample processing volume should not exceed the processing capacity of the genomic DNA removal column and RNA adsorption column to avoid DNA residue or yield reduction. The RNA\/DNA of various samples differs greatly, leading to a variation in the sample processing volume. When the experiment conditions are preliminarily explored, limit cell samples to 3 - 4 × 10 6 and tissue samples to 10 mg. Determine the processing volume for subsequent experiments based on the results.\u003c\/p\u003e\n\u003cp\u003e6. If residues are caused by excessive DNA content or rigorous fluorescent quantitative PCR is required, it is recommended to select intron-spanning primers or treat the RNAextract with RNase-free DNase.\u003c\/p\u003e\n\u003cp\u003e7. For the samples with excessively high DNAcontent, Step 6 can be performed as follows:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ea) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 30 s, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003eb) Add an appropriate amount of DNase working solution to the center of the adsorption column and place it at room temperature for 15 min.\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cem\u003ec) Add 350 μL of rinsing solution 1 to the adsorption column, centrifuge at 12,000 × g for 1 min, and discard the filtrate (putting the adsorption column into the collection tube).\u003c\/em\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003e8. Ensure that equipment used in the experiment is RNase-free, including gloves, pipette tips, EP tubes and mortars.\u003c\/p\u003e\n\u003cp\u003e9. Bake the glassware at 150°C for 4 hours. Soak the plasticware in 0.5 M NaOH for 10 min, wash it thoroughly with water, and sterilize it to remove RNase.\u003c\/p\u003e\n\u003cp\u003e10. The product is only intended for scientific research conducted by professionals. For your safety and health, please wear lab coats and disposable gloves during the operation.\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopifycdn.net\/s\/files\/1\/0521\/5312\/2997\/files\/8034111_803411T_BioSci_Animal_Tissue_Cell_Total_RNA_Extraction_Kit.pdf?v=1700038025\" type=\"application\/pdf\" target=\"_blank\"\u003eAnimal Tissue Cell Total RNA Extraction Kit\u003c\/a\u003e\u003c\/p\u003e","brand":"Dakewe","offers":[{"title":"50preps\/Kit","offer_id":43541462057141,"sku":"8034111","price":258.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/8034111BioSci_AnimalTissueCellTotalRNAExtractionKit_1.png?v=1732865877"},{"product_id":"residual-e-coli-dna-quantitation-kit-shentek®","title":"Residual E. coli DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', 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border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E. coli DNA Quantitation Kit is designed for quantitative measurement of residual E. coli host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (qPCR) to enable rapid and specific quantitation at the femtogram level. It is suitable for a broad range of sample types from in-process samples to final products. The kit includes an E. coli Control DNA traceable to a primary DNA reference standard and a ready-to-use qPCR mix. For nucleic acid extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as supplied with the kit).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (absolute quantitation using standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (standard curve)\u003c\/td\u003e\r\n \u003ctd\u003eStandard curve concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03 pg\/µL (recommended at least five concentration points).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eReported sensitivity: femtogram level (kit statement). Practical lowest standard used: 0.03 pg\/µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eqPCR reaction volume set to 30 µL (instrument setting described uses 30 µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/extension: 60 °C 01:00 (read fluorescence during this step).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended (ABI7500 SDS v1.4 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative and control acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NCS Ct should be larger than mean Ct of the lowest standard; NTC should be undetermined or Ct ≥ 35.00.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component labels).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus, Mx3000P, qTOWER3G, etc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Enables quantitation at the femtogram level; lowest standard shown at 0.03 pg\/µL.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTraceable control:\u003c\/strong\u003e Includes E. coli Control DNA traceable to a primary DNA reference standard for reliable standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Supplied E. coli qPCR MIX is provided in ready-to-use format to simplify reaction setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad sample compatibility:\u003c\/strong\u003e Validated conceptually for a variety of biopharmaceutical sample types from in-process to final products (recommended to perform method validation for specific matrices).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Protocol and plate layouts provided for a range of common real-time PCR instruments.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of residual host DNA during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing (research use applications)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli qPCR MIX (NNC002)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1 mL × 2 tubes (protect from light)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using a standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use RNase\/DNase-free consumables, low-retention filter tips, UV-decontaminate surfaces and pipettes, and use separate areas for pre- and post-amplification steps.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents as indicated.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations for a reliable standard curve and perform method validation for your sample matrix.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery range: 50%–150%; verify NTC is undetermined or Ct ≥ 35 and NCS Ct is larger than mean Ct of lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific software and parameter settings for data analysis (example settings provided for ABI 7500 SDS v1.4).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030202E100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research quantitation of residual E. coli host cell DNA in biopharmaceutical samples using qPCR (Research Use Only).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What are the standard curve concentrations supplied in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol describes preparing standards at 3000, 300, 30, 3, 0.3 and 0.03 pg\/µL (use at least five concentrations for the standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%; NTC should be undetermined or Ct ≥ 35; NCS Ct should be larger than the mean Ct of the lowest standard concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I set up the qPCR cycling program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended example: 95 °C for 10:00 (activation, 1 cycle); 95 °C for 0:15 (denaturation) and 60 °C for 1:00 (annealing\/extension with fluorescence read) for 40 cycles. Reaction volume example: 30 µL. Adjust program per instrument requirements.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186991784117,"sku":"SK030202E100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit.jpg?v=1742269328"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit (For manual and automated operation) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C or -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle separation-based sample preparation kit designed for efficient and reproducible extraction of trace residual host cell DNA from a wide range of biological products and complex matrices across different manufacturing steps. The kit is compatible with manual workflows or automated extraction using the rHCDpurify instrument and is intended for use prior to downstream host cell DNA quantitation and size analysis (e.g., with SHENTEK host cell DNA quantitation and size analysis kits). The kit includes reagents for enzymatic protein digestion (Proteinase K), binding, washing and elution of nucleic acids and is optimized for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. Product No.: 1104191. Reagents for 100 extractions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eExtraction of residual host cell DNA from biological products and complex sample matrices prior to quantitation and size analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume (example protocol)\u003c\/td\u003e\r\n \u003ctd\u003e100 µL sample per extraction (protocol example uses 100 µL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003ePrepare Proteinase K digestion solution per 100 µL sample: for sample protein 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; for 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer per sample\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (omit Yeast tRNA for E.coli and yeast DNA extractions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003e10 µL magnetic particles per sample; vortex to resuspend before use; recommended storage 2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIsopropanol\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 µL isopropanol per sample during binding step\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash steps\u003c\/td\u003e\r\n \u003ctd\u003eWash A: 700 µL Wash buffer A; Wash B: 700 µL Wash buffer B; remove residual liquid and air-dry pellet 30 s–3 min depending on environment\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003e50–100 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 min with intermittent mixing; centrifuge and separate on magnetic stand prior to transfer\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCritical temperatures and times\u003c\/td\u003e\r\n \u003ctd\u003eProteinase K digestion: 55 °C for 60 min; Elution: 70 °C for 7 min; vortex binding for 5 min; resuspend magnetic particles before use\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures can be kept up to 24 months (check labels for expiration dates)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH and ionic requirements\u003c\/td\u003e\r\n \u003ctd\u003eSample pH should be adjusted to neutral (pH 6.0–8.0). Adjust ionic concentration with 5M NaCl if needed.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with manual workflows and with the rHCDpurify instrument; downstream use with SHENTEK host cell DNA quantitation and size analysis kits and real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1104191\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic Particle Separation:\u003c\/strong\u003e Magnetic particle-based extraction provides efficient capture and purification of trace residual DNA from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad Host Cell Compatibility:\u003c\/strong\u003e Validated for multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh Recovery from Complex Matrices:\u003c\/strong\u003e Optimized binding, wash and elution steps including Proteinase K digestion to improve recovery from protein- or matrix-rich samples.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and Automated Workflows:\u003c\/strong\u003e Can be used in manual bench workflows or with the rHCDpurify automated instrument for higher throughput and consistency.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated Workflow Inputs:\u003c\/strong\u003e Includes reagent recipes and stepwise protocol (binding buffer composition, enzyme digestion volumes, wash volumes and temperatures) for reproducible extraction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady for Downstream qPCR or Size Analysis:\u003c\/strong\u003e Eluted DNA is suitable for downstream host cell DNA quantitation and size analysis workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell DNA from biologics and bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to host cell DNA quantitation (e.g., qPCR)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation prior to DNA size\/fragment analysis\u003c\/li\u003e\r\n \u003cli\u003eQuality control and in-process monitoring of upstream and downstream manufacturing steps\u003c\/li\u003e\r\n \u003cli\u003eRecovery of trace DNA from complex matrices (formulations, buffers, cell lysates, clarified harvests)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 40 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND018)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND021)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy heat at 37 °C to dissolve)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1\u003c\/span\u003e\u003cstrong\u003e-20 °C (omit for E.coli and yeast DNA extractions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based DNA extraction\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL anhydrous ethanol to Wash buffer A before first use and prepare Wash buffer B as 70% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Adjust sample pH to neutral (pH 6.0–8.0) with 1M HCl or 1M NaOH prior to extraction.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution according to sample protein concentration; digestion at 55 °C for 60 min is critical for DNA recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when extracting E. coli or yeast DNA.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly immediately before use; avoid over-drying the pellet (30 s–3 min) to ensure efficient elution.\u003c\/p\u003e\r\n \u003cp\u003e• Perform centrifugation immediately after vortexing to collect liquid and particles from tube walls and caps.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day after nucleic acid extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; storing at -20 °C may reduce performance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104191.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with a wide range of sample matrices from multiple host cell types including CHO, E. coli, Vero, yeast, NS0, Human, MDCK, Sf9 \u0026amp; AcNPV, Hi5 \u0026amp; AcNPV, plasmid, SV40LTA \u0026amp; EIA. It is intended for biological products at various manufacturing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample volume is used in the protocol?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided example protocol uses 100 µL sample per extraction. Adjustments may be made but follow recommended binding and enzyme volumes for optimal recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare Proteinase K digestion solution?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFor each 100 µL sample: if sample protein is 0–100 mg\/mL add 10 µL Proteinase K to 100 µL Proteinase K Buffer; if sample protein is 100–200 mg\/mL add 20 µL Proteinase K to 100 µL Proteinase K Buffer. Incubate at 55 °C for 60 min.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I use this kit with automated instruments?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with manual workflows and automated extraction using the rHCDpurify instrument; ensure instrument settings follow the recommended incubation times and mixing steps.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45186973237429,"sku":"1104191","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_manual_and_automated_operation.jpg?v=1742277004"},{"product_id":"residual-e-coli-dna-quantitation-kit-2g-shentek™","title":"Residual E. coli DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli DNA Quantitation Kit (2G) is a duplex real‑time PCR (qPCR) assay designed to quantitate residual Escherichia coli host cell DNA in biopharmaceutical samples from in‑process material to final products. The kit provides high sensitivity (fg level), rapid and specific absolute quantitation using an internal positive control (IPC) included in the Primer \u0026amp; Probe mix to monitor PCR performance. The kit includes DNA control, qPCR master mix, DNA dilution buffer and Primer \u0026amp; Probe mix (with IPC). For extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Supplied reagents are sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical samples (in‑process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real‑time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReported sensitivity at the fg level (document states fg level performance)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared serial dilutions: ST0 3000 pg\/µL (stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume (program)\u003c\/td\u003e\r\n \u003ctd\u003eThermal cycling reaction volume set to 30 µL (instrument setting referenced)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15, Annealing\/Extension 60 °C 1:00, 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt thresholds \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct used. Thresholds: E.coli‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC sample recovery: 50%–150%. NTC Ct ≥ 35.00. NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. IPC monitored in VIC channel.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under specified conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, LightCycler 480 (and other compatible real‑time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real‑time PCR:\u003c\/strong\u003e Simultaneous detection of E. coli target and IPC in the same reaction for reliable quantitation and internal reaction control.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual E. coli DNA down to the fg level using an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use components:\u003c\/strong\u003e Includes DNA control, qPCR Master Mix, Primer \u0026amp; Probe mix (with IPC) and DNA Dilution Buffer for streamlined workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC acceptance criteria:\u003c\/strong\u003e Built‑in criteria for NTC, NCS and ERC recovery (50%–150%) to support routine QC and method validation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument support:\u003c\/strong\u003e Validated for common real‑time PCR instruments (ABI 7500, CFX96, LightCycler 480, SHENTEK‑96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E. coli host cell DNA in biopharmaceutical raw materials, intermediates and final products\u003c\/li\u003e\r\n \u003cli\u003eProcess development and in‑process monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of biologics for host cell DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and CRO testing workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli DNA Control (NNA002) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli Primer \u0026amp; Probe MIX (incl. IPC) (NNC115) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDSs) before use; wear appropriate protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNA‑free consumables; perform UV decontamination of tabletop, pipettes and tubes for 30 minutes and disinfect surfaces with 75% ethanol to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and spin briefly. Protect light‑sensitive reagents (Master MIX and Primer \u0026amp; Probe MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C. Unused DNA Dilution Buffer (DDB) may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (standard curve, NTC, NCS, ERC) and run samples in replicates (recommended triplicates).\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument‑specific settings when adapting the qPCR program; provided cycling parameters and analysis thresholds are validated on ABI 7500 SDS v1.4 and may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101107-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity \/ limit of detection (LOD) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe user guide reports assay performance at the fg level (ultra‑sensitive); exact numeric LOD is not specified in the document and should be determined during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided in one kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the supplied E. coli DNA Control to generate at least five standard points. Example dilutions in the guide: ST1 300 pg\/µL, ST2 30 pg\/µL, ST3 3 pg\/µL, ST4 0.3 pg\/µL, ST5 0.03 pg\/µL. Include standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC Ct should be ≥ 35.00. NCS Ct should be greater than the mean Ct of the lowest standard and show normal amplification in the VIC (IPC) channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45186998108341,"sku":"1101107-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E._coli_DNA_Quantitation_Kit_2G.jpg?v=1742277494"},{"product_id":"residual-cho-dna-quantitation-kit-2g-shentek™","title":"Residual CHO DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit (2G) is intended to quantitate host-cell line residual DNA from CHO cell lines used in biopharmaceutical production. The kit uses duplex real-time PCR (qPCR) technology to perform rapid, specific and reliable quantitation down to the fg level. An Internal Positive Control (IPC) is included in the CHO Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit provides ready-to-use qPCR Master Mix, CHO Primer \u0026amp; Probe MIX with IPC, a quantified CHO DNA Control for standard curve generation, and DNA Dilution Buffer. For DNA extraction instructions, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Product No.: 1101100-1. Reagents for 100 reactions. For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (label on kit: Reagents for 100 Reactions).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (serial dilutions)\u003c\/td\u003e\r\n \u003ctd\u003eST0 3000 pg\/μL (stock), ST1 300 pg\/μL, ST2 30 pg\/μL, ST3 3 pg\/μL, ST4 0.3 pg\/μL, ST5 0.03 pg\/μL, ST6 0.003 pg\/μL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e3000 pg\/μL to 0.003 pg\/μL (nominal dynamic range as provided by standard series).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD (document statement)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims quantitation at the fg level; lowest provided standard is 0.003 pg\/μL (3 fg\/μL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup (per well)\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX (15 μL qPCR Master MIX + 5 μL CHO Primer\u0026amp;Probe MIX) + 10 μL sample\/standard\/NTC\/NCS = 30 μL final reaction volume.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extension, fluorescence read).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels and dyes\u003c\/td\u003e\r\n \u003ctd\u003eCHO-DNA detector: FAM reporter; IPC detector: VIC reporter; Passive reference: ROX.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected. Threshold: CHO-DNA = 0.05, IPC = 0.1. Automatic baseline recommended. Instrument-specific settings may vary.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptability criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35.00 (or laboratory-specific threshold). NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification. ERC recovery should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit components storage conditions\u003c\/td\u003e\r\n \u003ctd\u003eAll listed components stored at -20 °C; protect light where indicated. Kit stable up to 24 months (check label expiration).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ version \/ manufacturer\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1101100-1. User Guide Version: A\/1. Effective date: 01 Jul. 2024. Manufacturer: Biofargo, Inc.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR (qPCR):\u003c\/strong\u003e Simultaneous detection of CHO target sequence and an internal positive control (IPC) in the same reaction to monitor assay performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at the femtogram level; includes a dilution series down to 0.003 pg\/μL (3 fg\/μL) standard.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and CHO Primer \u0026amp; Probe MIX (with IPC) minimize assay setup time and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for common real-time PCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Roche LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControls included:\u003c\/strong\u003e Includes quantified CHO DNA Control for standard curve generation, DNA Dilution Buffer (DDB), and IPC in the primer\/probe mix.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host-cell DNA in CHO-derived biopharmaceuticals\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and process development for biologics produced in CHO cells (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control assays in research settings where CHO DNA detection is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer \u0026amp; Probe MIX (including IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five standard curve points; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct acceptance: ≥ 35.00 (or lab-specific threshold). NCS Ct should be greater than mean Ct of lowest standard and display normal VIC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• ERC (extraction reference control) recovery must be between 50% and 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101100-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for quantitation at the femtogram level. The lowest supplied standard is 0.003 pg\/μL (3 fg\/μL); actual limit of detection should be confirmed by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction (15 μL qPCR Master MIX + 5 μL CHO Primer \u0026amp; Probe MIX) and add 10 μL sample or standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which fluorescence channels are used for target and control?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCHO target is detected in the FAM channel; the internal positive control (IPC) is detected in the VIC channel; ROX is used as passive reference when applicable.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35.00 (or a lab-defined threshold). NCS Ct should be higher than the mean Ct of the lowest standard and show VIC amplification. ERC recovery should fall between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187351445685,"sku":"1101100-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit_2G.jpg?v=1742278220"},{"product_id":"residual-hek293-dna-quantitation-kit-3g-shentek™","title":"Residual HEK293 DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual HEK293 DNA Quantitation Kit (3G) is designed to quantify residual HEK293 host cell DNA in biopharmaceutical samples at various stages (in‑process to final product). The kit uses duplex real‑time PCR (qPCR) for rapid, specific and reliable quantitation at the fg level. An Internal Positive Control (IPC) is included in the HEK293 Primer \u0026amp; Probe MIX to evaluate PCR performance. The kit supports extraction workflows (refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit, Product No. 1104191) and is supplied as ready‑to‑use master mix and primer\/probe reagents for up to 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDuplex real‑time PCR (qPCR) with target (FAM) and IPC (VIC) channels\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e20 μL qPCR MIX + 10 μL sample\/control = 30 μL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ LOD guidance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit claims quantitation at the fg level. Lowest standard: 0.03 pg\/μL (30 fg\/μL). NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific limit based on validation).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery \/ Accuracy guidance\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (example for ABI7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct. Thresholds: HEK293‑DNA = 0.05; IPC = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, BioRad CFX96, Roche LightCycler 480 and other real‑time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims quantitation at the femtogram (fg) level with standard curve down to 0.03 pg\/μL (30 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (IPC) in the Primer \u0026amp; Probe MIX to monitor inhibition and reaction performance in each well.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady‑to‑use reagents:\u003c\/strong\u003e Supplied as qPCR Master MIX and premixed Primer \u0026amp; Probe, minimizing setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated on multiple real‑time PCR platforms (e.g., SHENTEK‑96S, ABI7500, CFX96, LightCycler 480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharmaceutical testing:\u003c\/strong\u003e Optimized for residual host cell DNA measurement across in‑process and final product samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in upstream and downstream bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing for biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn‑process and final product residual DNA testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (850 μL × 2 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer \u0026amp; Probe MIX (Includes IPC)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light (500 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, lab coat, protective eyewear, mask) and work in nuclease‑free conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and consumables by UV irradiation (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge. Protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• Perform laboratory‑specific validation to establish LOD, LOQ and acceptance criteria.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure plate layout includes appropriate standards (at least five concentrations), NTC, NCS and ERC samples; run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101104-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the claimed sensitivity of the kit and what are the lowest standard concentrations?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit claims quantitation at the femtogram (fg) level. The provided standard curve points include ST1 = 300 pg\/μL down to ST5 = 0.03 pg\/μL (30 fg\/μL). Laboratories should perform validation to determine actual LOD\/LOQ in their workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I set up reaction volumes and plate layout?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 20 μL qPCR MIX per reaction and add 10 μL of sample\/control to each well for a total reaction volume of 30 μL. Include at least five standard points, one NTC, one NCS and ERCs as described; triplicate wells are recommended for each sample.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended cycling conditions and analysis settings?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample conditions (ABI7500 SDS v1.4): Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 1:00 with fluorescence read during the 60 °C step. Suggested thresholds: HEK293‑DNA = 0.05; IPC = 0.1; use manual Ct and automatic baseline or adapt per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should I use for ERC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC detection value should be ≤ 6.00 fg\/μL (or laboratory‑specific standard based on validation). The Ct of NCS should be larger than the mean Ct of the lowest standard and show IPC amplification in the VIC channel.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189158633653,"sku":"1101104-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Quantitation_Kit_3G.jpg?v=1742279848"},{"product_id":"residual-plasmid-dna-quantitation-kit-3g-shentek™","title":"Residual Plasmid DNA Quantitation Kit (3G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Plasmid DNA Quantitation Kit (3G) is a duplex real-time PCR (qPCR) kit designed to quantify residual plasmid DNA in gene therapy products (for example, plasmid DNA in lentiviral vector preparations used in CAR‑T manufacturing). The assay targets consensual plasmid sequences (replicon sequences of ColE1\/pMB1\/pBR322\/pUC) and uses a FAM-labeled target probe for quantitative detection and a VIC-labeled Internal Positive Control (IPC) included in the Primer\u0026amp;Probe MIX to monitor reaction performance. The kit provides reagents and a lyophilized plasmid linear DNA control for generation of a standard curve, enabling reliable quantitation down to the 10^2 copies\/µL level. The kit is supplied for 100 reactions and is intended for research use only. Compatible with common real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 1×10^2 copies\/µL (sensitivity reported by manufacturer)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitative dynamic range \/ Standard curve\u003c\/td\u003e\r\n \u003ctd\u003eSerial dilution standard curve covers 4.97×10^8 to 4.97×10^1 copies\/µL (prepared from stock A down through ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total (20 µL qPCR MIX + 10 µL sample\/standard\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Master MIX 15 µL + Plasmid Primer\u0026amp;Probe MIX (incl. IPC) 5 µL = 20 µL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 01:00 (anneal\/extend; fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection channels \/ probes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget probe: FAM (Plasmid-DNA); Internal Positive Control (IPC): VIC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct threshold: Plasmid-DNA (FAM) = 0.05; IPC (VIC) = 0.1. Automatic baseline recommended.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate acceptance: 50%–150%; NTC Ct should be ≥ (ST5 Ct + 2) or lab-specific; NCS Ct should be larger than mean Ct of lowest standard and show normal VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (components stable for up to 24 months under recommended storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex qPCR with IPC:\u003c\/strong\u003e Includes an Internal Positive Control (VIC) within the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition or reaction failure.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Reported detection sensitivity at the 10^2 copies\/µL level for trace plasmid DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eConsensus plasmid sequence targeting:\u003c\/strong\u003e Assay targets consensual replicon sequences (ColE1\/pMB1\/pBR322\/pUC) to detect a broad range of common plasmid backbones.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents and controls:\u003c\/strong\u003e Includes lyophilized plasmid linear DNA control and pre-mixed Primer\u0026amp;Probe and Master MIX reagents to streamline setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio‑Rad CFX96, Roche LightCycler 480 and similar platforms).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow for regulated testing environments:\u003c\/strong\u003e Provides recommended standard curve design, plate layouts, and acceptance criteria to support method validation and sample testing.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual plasmid DNA in gene therapy products\u003c\/li\u003e\r\n \u003cli\u003eMonitoring plasmid DNA residue in lentiviral vector preparations (CAR‑T production workflows)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for biologics where plasmid backbone contamination must be measured\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and process development for plasmid removal\/clearance\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid linear DNA Control (NNA016), lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePlasmid Primer\u0026amp;Probe MIX (Incl. IPC) (NNC120), 500 µL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023), 850 µL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (duplex real-time PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or clinical use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and observe all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves) when handling reagents and samples.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention tubes and tips to avoid contamination and sample loss.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes with UV (30 minutes) and 75% ethanol before experiments to minimize nucleic acid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store all kit components at -20 °C. Unused DNA Dilution Buffer (DDB) should be stored at 2–8 °C; clear cloudy DDB by warming to 37 °C if necessary.\u003c\/p\u003e\r\n \u003cp\u003e• Include recommended controls (NTC, NCS, ERC, and standard curve points) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Follow the provided plate layouts, thermal profile and analysis settings; adjust thresholds\/criteria only after laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101111-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect residual plasmid DNA down to approximately 1×10^2 copies\/µL. Actual limit of detection should be confirmed by the user's in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDissolve the lyophilized Plasmid linear DNA Control in 55 µL ddH2O, dilute to the stock concentration indicated on the tube, then perform serial 1:10 dilutions to generate standards A → ST5. Recommended standard points in the manual range from 4.97×10^8 to 4.97×10^1 copies\/µL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I interpret ERC recovery and control criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC (extraction reference control) recovery rates should fall between 50% and 150%. NCS Ct should be larger than the mean Ct of the lowest standard and show VIC amplification; NTC Ct should be ≥ ST5 Ct + 2 (or per your lab validation).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should the kit be stored and what is the shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore all kit components at -20 °C and protect light-sensitive reagents from light. Components are stable for up to 24 months when stored under recommended conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189159813301,"sku":"1101111-1","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Plasmid_DNA_Quantitation_Kit_3G.jpg?v=1742288363"},{"product_id":"residual-vero-dna-size-analysis-kit-2g-shentek™","title":"Residual Vero DNA Size Analysis Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA Size Analysis Kit (2G) is a real-time PCR (qPCR) based assay for rapid and specific quantitation of residual Vero (host cell) DNA fragments across multiple size ranges in biopharmaceutical samples (in-process to final product). The kit is designed to amplify four discrete fragment lengths (85 bp, 134 bp, 229 bp and 552 bp) and determine size distribution and quantity down to the femtogram (fg) level. Includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, DNA dilution buffer (DDB) and a Vero DNA control for generating standard curves. Reagents are provided for 4 × 100 reactions. For sample extraction and preparation refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e85 bp, 134 bp, 229 bp, 552 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReal-time PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported sensitivity \/ limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects and quantifies Vero DNA down to the femtogram (fg) level (standard curve includes concentrations down to 0.03 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (per kit dilution scheme)\u003c\/td\u003e\r\n \u003ctd\u003eST0 (prepared) 3000 pg\/µL; ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: 20 µL qPCR MIX + 10 µL template\/standard\/NTC (total 30 µL reaction volume). qPCR MIX composition per reaction: qPCR Reaction Buffer 15.9 µL; Vero Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL; total qPCR MIX 20 µL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15; Annealing 60 °C 00:30; Extension 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control (IPC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eSample Ct(IPC) should be within ±1.0 Ct of NCS Ct(IPC). Significant increase indicates potential inhibition; evaluate recovery with ERC samples.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 2 Ct higher than mean Ct of the lowest standard concentration, or laboratory-specific acceptance criteria established during validation.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months; check label expiration date.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation and size-distribution analysis of residual Vero DNA in biopharmaceutical samples (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-fragment size analysis:\u003c\/strong\u003e Four specific primer\/probe assays amplify 85 bp, 134 bp, 229 bp and 552 bp fragments to determine size distribution of residual DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify residual DNA at femtogram-level sensitivity using absolute quantitation and serial standard curves.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBuilt-in internal control:\u003c\/strong\u003e IPC mix included to monitor inhibition and reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR reaction buffer and primer\/probe mixes minimize setup variability and speed workflow.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600plus) and adjustable to other qPCR instruments following instrument software guidance.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero (host cell) DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA fragment size distribution during downstream processing and in final product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and release testing in research and development (RUO) workflows\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery studies for host cell DNA assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-85 (NNC020) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-134 (NNC021) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-229 (NNC022) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-552 (NNC023) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069) 550 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eStore per label (typically -20 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProvided documentation\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 4 × 100 reactions (400 reactions total) | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read MSDS and user guide before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-decontaminate workspace, use DNase-free consumables, aliquot reagents and change tips between samples.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents as labeled; check expiration date on labels.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific settings and analysis parameters; adjust thresholds\/baselines per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: standards (ST1–ST5), NTC, NCS and IPC for each run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103174.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe assay is designed for femtogram-level sensitivity. The supplied standard curve includes concentrations down to 0.03 pg\/µL (ST5). Exact LOD\/LOQ should be established during laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500 (with ROX), Bio-Rad CFX96 and LineGene 9600plus. Other real-time PCR instruments are supported; follow instrument-specific setup and dye assignments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How are results interpreted and what are acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSet up an absolute quantitation standard curve (ST1–ST5). IPC Ct for samples should be within ±1.0 of the NCS IPC Ct; significant deviation indicates inhibition. NTC Ct should be at least 2 Ct higher than the mean Ct of the lowest standard (or per laboratory acceptance criteria).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw Vero DNA Control and DDB on ice. Prepare ST0 at 3000 pg\/µL using DDB, then perform serial 1:10 dilutions to obtain ST1=300 pg\/µL, ST2=30 pg\/µL, ST3=3 pg\/µL, ST4=0.3 pg\/µL and ST5=0.03 pg\/µL. Include at least five standard points (suggested ST1–ST5) in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189155881141,"sku":"1103174","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Size_Analysis_Kit_2G.jpg?v=1742290678"},{"product_id":"residual-hek293-dna-size-analysis-kit-shentek™","title":"Residual HEK293 DNA Size Analysis Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual HEK293 DNA Size Analysis Kit is designed to quantify residual HEK293 host cell DNA fragments of different sizes across biopharmaceutical sample types (in-process samples to final products) using real-time PCR (qPCR). The kit amplifies four fragment sizes (75 bp, 122 bp, 244 bp and 562 bp) to determine size distribution and quantity down to the femtogram (fg) level. The kit includes primer\/probe mixes for each fragment, internal positive control (IPC), DNA dilution buffer and a HEK293 DNA control for standard curve preparation. For extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e75 bp, 122 bp, 244 bp, 562 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eReal-time PCR (qPCR) with TaqMan-style primer \u0026amp; probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned for quantitation at the femtogram (fg) level (lowest standard in kit workflow corresponds to 0.03 pg\/µL = 30 fg)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (prepared from supplied HEK293 DNA Control)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock prior to dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLinear \/ working range (as provided by recommended standards)\u003c\/td\u003e\r\n \u003ctd\u003eDemonstrated standard points from 0.03 pg\/µL to 300 pg\/µL (prepare standard curve using ST1–ST5); ST0 (3000 pg\/µL) used as stock\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR master mix + 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR master mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; HEK293 Primer\u0026amp;Probe MIX (appropriate fragment) 2.8 μL; IPC MIX 1.3 μL; total 20 μL (then add 10 μL template)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/read: 72 °C 01:30; 40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, FQD-96A and similar real-time PCR instruments (user must configure detectors\/channels accordingly)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC \/ quality control criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. Ct of NTC and NCS should be larger than the mean Ct of the lowest concentration standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability \/ shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under appropriate conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSize-resolved quantitation:\u003c\/strong\u003e Simultaneous assays for four discrete fragment lengths (75, 122, 244, 562 bp) allow determination of residual HEK293 DNA size distribution.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to quantify DNA down to the femtogram range (lowest recommended standard 0.03 pg\/µL = 30 fg).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer \u0026amp; probe mixes:\u003c\/strong\u003e Pre-formulated HEK293 Primer\u0026amp;Probe MIXes for each fragment size reduce setup time and minimize variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control (IPC):\u003c\/strong\u003e IPC MIX included to monitor inhibition and assay performance; acceptance criteria provided.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible instrument compatibility:\u003c\/strong\u003e Compatible with multiple real-time PCR platforms (instructions include detector\/channel setup and reference dye settings).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA dilution buffer and HEK293 DNA control for standard curve preparation; refers to complementary sample prep kit for extraction.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual HEK293 host cell DNA in biopharmaceutical process and final product testing\u003c\/li\u003e\r\n \u003cli\u003eDetermination of host cell DNA fragment size distribution\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring and process development for biologics produced in HEK293 cells\u003c\/li\u003e\r\n \u003cli\u003eRelease testing and quality control (research-use contexts)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 μL × 8 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-75 (NNC012), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-122 (NNC013), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-244 (NNC014), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 Primer\u0026amp;Probe MIX-562 (NNC015), 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069), 550 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after thaw; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHEK293 DNA Control (NNA005), 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment-specific primer\/probe mix (75, 122, 244, 562 bp).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points (recommended ST1–ST5) and run samples in triplicate where possible.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR run setup: reaction volume 30 μL (20 μL master mix + 10 μL template); thermal cycling and detector settings must be configured per instrument.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: sample Ct-IPC should be within ±1.0 of NCS Ct-IPC — higher Ct-IPC indicates potential inhibition; consider recovery controls.\u003c\/p\u003e\r\n \u003cp\u003e• Negative controls (NTC) and NCS should show Ct values greater (later) than the mean Ct of the lowest standard point.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103176.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity \/ limit of detection (LOD) of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to quantify HEK293 DNA at the femtogram level. The supplied standard curve includes points down to 0.03 pg\/µL (30 fg); actual LOD\/LOQ should be established during method validation in your laboratory.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which fragment sizes are measured and how are they assayed?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eFour fragment sizes are assayed separately using fragment-specific primer \u0026amp; probe mixes: 75 bp, 122 bp, 244 bp and 562 bp. Prepare four separate standard curves (one per fragment) and run each assay on the qPCR instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems such as SHENTEK-96S, Applied Biosystems 7500, Bio-Rad CFX96, and FQD-96A. Users must configure detectors\/channels, passive reference dye (e.g., ROX if required), and cycling conditions according to their instrument.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I know if a sample is inhibited?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eCheck the IPC (internal positive control). The sample Ct-IPC should be within ±1.0 of the NCS Ct-IPC. A significantly higher Ct-IPC in the sample suggests inhibition; dilute the sample or perform recovery controls and re-test.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45189170954421,"sku":"1103176","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_HEK293_DNA_Size_Analysis_Kit.jpg?v=1742291951"},{"product_id":"residual-host-cell-rna-sample-preparation-kit-shentek™","title":"Residual Host Cell RNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature; 2-8 °C; -20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell RNA Sample Preparation Kit is a magnetic particle-based RNA extraction kit for isolation of residual host cell RNA from biological products (e.g., E. coli, 293T). The kit is compatible with manual workflows and automated extraction on the rHCDpurify system. Extracted RNA is suitable for downstream RNA quantitation (real-time qPCR) and other nucleic acid-based assays. The kit includes reagents for protein digestion (Proteinase K), binding, washing, magnetic particles, RNase inhibitor, glycogen carrier, and elution reagents. The kit is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eExtraction of residual host cell RNA from biological samples for downstream RNA quantitation (qPCR)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit capacity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMagnetic particle-based RNA binding, wash and elution\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion\u003c\/td\u003e\r\n \u003ctd\u003eTypical: add 100 µL sample + 110 µL Proteinase K digestion solution; incubate 55 °C for 60 min; machine max digestion volume 300 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K dosing guidance\u003c\/td\u003e\r\n \u003ctd\u003eFor sample protein concentration 0–100 mg\/mL: 10 µL Proteinase K per sample; for 100–200 mg\/mL: 20 µL Proteinase K per sample (volumes mixed with Proteinase K Buffer as tabled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding\u003c\/td\u003e\r\n \u003ctd\u003ePer sample: add 10 µL 5M NaCl + 220 µL working binding solution; add 200–400 µL isopropanol (≈1\/3–1\/2 total volume); add 10–15 µL magnetic particles; mix 3–5 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWashing\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL; air-dry magnetic particles 30 s–3 min to remove residual ethanol\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAdd 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v); incubate 50 °C for 5 min with intermittent vortexing; separate beads and recover eluate\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles\u003c\/td\u003e\r\n \u003ctd\u003eUse 10–15 µL per sample; vortex to re-suspend before use; recommended storage 2–8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking binding prep\u003c\/td\u003e\r\n \u003ctd\u003ePer sample working binding: 200 µL Binding solution + 10 µL diluted Precipitation solution I (1:99 in Dilution solution) + 10 µL Glycogen\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003epH requirement\u003c\/td\u003e\r\n \u003ctd\u003eIdeal sample pH 6.0–8.0; adjust with 1 M HCl or 1 M NaOH if pH \u0026lt;5.0 or \u0026gt;9.0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at indicated temperatures are stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatibility \/ equipment\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with rHCDpurify automated system and manual extraction; requires real-time PCR system for downstream quantitation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle-based separation:\u003c\/strong\u003e Efficient magnetic bead capture of RNA for high recovery and clean eluates suitable for qPCR\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAutomated and manual compatibility:\u003c\/strong\u003e Validated for use with rHCDpurify instrument and a manual workflow\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for RNA extraction:\u003c\/strong\u003e Includes Proteinase K digestion reagents, binding\/wash\/elution buffers, magnetic particles, RNase inhibitor and glycogen carrier\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for residual host cell RNA:\u003c\/strong\u003e Optimized procedure for extraction of residual RNA from biological products and plasmid preparations\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStable shelf life:\u003c\/strong\u003e Components stored at proper temperatures are stable up to 24 months\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of residual host cell RNA from biopharmaceutical samples (e.g., E. coli, 293T)\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for RNA quantitation by real-time qPCR\u003c\/li\u003e\r\n \u003cli\u003ePreparation of plasmid DNA samples with residual host RNA assessment\u003c\/li\u003e\r\n \u003cli\u003eRoutine QC workflows in research and development laboratories\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND026) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND016) 20 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND014) 30 mL × 1 (requires addition of 40 mL ethanol before first use)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution solution (NND061) 10 mL × 1\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution solution (NND062) 12 mL × 2\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase inhibitor (NND060) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePrecipitation solution I (NND003) 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 extractions | Method: Magnetic particle-based RNA extraction (manual or rHCDpurify automated)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: gloves, protective eyewear, mask and lab clothing.\u003c\/p\u003e\r\n \u003cp\u003e• Add 40 mL ethanol to Wash buffer A before first use; prepare 70% ethanol Wash buffer B from Dilution solution.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare working elution solution by mixing RNase inhibitor : Elution solution = 1 : 200 (v:v) in RNase-free area.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare diluted Precipitation solution I at 1:99 (Precipitation solution I : Dilution solution) before preparing working binding solution.\u003c\/p\u003e\r\n \u003cp\u003e• Ideal sample pH is 6.0–8.0. Adjust samples to neutral pH if outside range.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry magnetic particles; over-drying may reduce elution efficiency.\u003c\/p\u003e\r\n \u003cp\u003e• Perform downstream assays the same day as extraction to ensure accurate results.\u003c\/p\u003e\r\n \u003cp\u003e• When removing supernatant, do not disturb or aspirate magnetic bead pellet; use low-retention tips and centrifuge as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• UV sterilize rHCDpurify instrument interior (suggested 15 minutes) before runs; allow \u0026gt;30 min between extractions if required.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201205.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201205.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What downstream assays are compatible with the extracted RNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted RNA is suitable for RNA quantitation by real-time qPCR and other nucleic-acid based assays. Use appropriate DNase treatment if required by your assay.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How do I prepare Wash buffer A and B before first use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 40 mL of ethanol to Wash buffer A (NND014) before first use. Prepare Wash buffer B by mixing Dilution solution (NND062) with ethanol to make 70% ethanol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended elution volumes and conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eElute with 50–100 µL working elution solution (RNase inhibitor:Elution = 1:200 v:v). Incubate at 50 °C for 5 minutes with intermittent vortexing, then separate beads and recover eluate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can this kit be used on an automated platform?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify automated extraction system (program rHCR-1201205) as well as manual extraction workflows.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Extractions","offer_id":45189191270581,"sku":"1201205","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_RNA_Sample_Preparation_Kit.jpg?v=1742293165"},{"product_id":"residual-e-coli-rna-quantitation-kit-2g-shentek™","title":"Residual E.coli RNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqRT-PCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual E. coli RNA Quantitation Kit (2G) is designed for quantitation of host cell RNA from Escherichia coli in biopharmaceutical products. The kit employs a duplex reverse transcription quantitative PCR (qRT-PCR) format including a target detector (FAM) and an Internal Positive Control (IPC, VIC) in the E. coli RNA Primer \u0026amp; Probe MIX. The assay provides rapid, specific and reliable quantitation with femtogram-level sensitivity for the target gene. The kit includes One Step qPCR Buffer, One Step Enzyme MIX, Primer \u0026amp; Probe MIX (incl. IPC), E. coli RNA Control and RNase-free water, and is compatible with common real-time PCR instruments.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry\u003c\/td\u003e\r\n \u003ctd\u003eDuplex one-step reverse transcription quantitative PCR (one-step RT-qPCR) with FAM-labeled target and VIC-labeled IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ Limit of detection\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eTarget detectable at femtogram (fg) level (document states target gene can be determined at the femtogram level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared from 2000 pg\/µL (stock) producing working standards: 20, 2, 0.2, 0.02, 0.002 pg\/µL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 20 µL (15 µL qRT-PCR MIX + 5 µL sample\/standard\/controls)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqRT-PCR mix composition per well\u003c\/td\u003e\r\n \u003ctd\u003eOne Step qPCR Buffer 10 µL, One Step Enzyme MIX 1 µL, E. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) 4 µL — total 15 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eReverse transcription 50 °C 15:00 (1 cycle); Activation 95 °C 0:30 (1 cycle); 45 cycles of 95 °C 0:10 (denature) and 60 °C 0:40 (anneal\/extend, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold 0.05; Automatic baseline; ROX as passive reference (for ABI 7500), set Standard Curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be at least 2 cycles higher than the lowest standard; ERC recovery acceptance 50%–150%; compare sample IPC Ct to NTC\/NCS IPC Ct to evaluate inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase digestion conditions (for plasmid samples)\u003c\/td\u003e\r\n \u003ctd\u003eIncubate samples with DNase I at 37 °C for 30–60 minutes; recommended DNase I final concentration 0.2–2 U\/µL depending on sample; ensure plasmid sample ≤100 ng\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDNase inactivation\u003c\/td\u003e\r\n \u003ctd\u003eOptions: (1) SHENTEK® Residual Host Cell RNA Sample Preparation Kit purification; (2) heat inactivation at 75 °C for 10 minutes; (3) other validated methods\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability (kit)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStore kit components at –20 °C; kit components stable up to 24 months (check label expiration)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex one‑step RT-qPCR:\u003c\/strong\u003e Combined reverse transcription and qPCR in a single reaction for speed and reduced handling\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Target detection at femtogram (fg) level enabling quantitation of trace residual E. coli RNA\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included in Primer \u0026amp; Probe MIX (VIC) to monitor PCR performance and potential inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated buffer, enzyme mix and primer\/probe mix to simplify setup and improve reproducibility\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflow compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II, Bio-Rad CFX96)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow for biopharmaceutical samples:\u003c\/strong\u003e Protocols and optional sample prep kit provided for plasmid DNA and protein expression products\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual host cell RNA (HCR) from Escherichia coli in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in plasmid DNA preparations\u003c\/li\u003e\r\n \u003cli\u003eResidual RNA testing in protein expression products derived from E. coli\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and in-process or release testing for downstream product quality control\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Control (NNA011) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step qPCR Buffer (NNB008) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eOne Step Enzyme MIX (NNC052) 100 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE. coli RNA Primer \u0026amp; Probe MIX (incl. IPC) (NNC119) 400 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eRNase-Free H2O (NND008) 1.2 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: Reagents for 100 Reactions | Method: qRT-PCR (duplex one-step reverse transcription quantitative PCR)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive components (buffer and enzyme mix) from light; store all kit components at –20 °C and check expiration date.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice and mix gently; avoid repeated freeze-thaw cycles.\u003c\/p\u003e\r\n \u003cp\u003e• Work in RNase-free conditions: use RNase\/DNase-free consumables, decontaminate surfaces and irradiate equipment with UV for 30 minutes, disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Perform DNase I treatment to remove genomic DNA for plasmid-derived samples; incubate at 37 °C for 30–60 minutes and inactivate DNase I (e.g., 75 °C for 10 minutes or purification).\u003c\/p\u003e\r\n \u003cp\u003e• Ensure final plasmid concentration ≤100 ng\/µL when performing DNase digestion; use DNase I final concentration 0.2–2 U\/µL as appropriate.\u003c\/p\u003e\r\n \u003cp\u003e• Include controls: standards (ST1–ST5), NTC, NCS and ERC; ERC recovery should be within 50%–150% to accept sample results.\u003c\/p\u003e\r\n \u003cp\u003e• If sample IPC Ct is significantly higher than NTC\/NCS IPC Ct, sample inhibition is possible; interpret IPC as reference and consider sample recovery.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1201201-1.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is reported to detect the target gene at the femtogram (fg) level. Use the provided standard curve (typical working standards: 20 to 0.002 pg\/µL) to determine assay sensitivity in your system and matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, Applied Biosystems 7500, Roche LightCycler 480 II and Bio-Rad CFX96. Program settings may require adjustment per instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples derived from plasmid DNA be prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePlasmid-derived samples require DNase I digestion to remove genomic DNA (37 °C for 30–60 min). After digestion, inactivate or remove DNase I (e.g., heat 75 °C for 10 min or use the SHENTEK Residual Host Cell RNA Sample Preparation Kit) before qRT-PCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude a standard curve (ST1–ST5), no template control (NTC), negative control sample (NCS), and extraction recovery control (ERC). NTC Ct should be ≥2 cycles above the lowest standard; ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45189194055861,"sku":"1201201-1","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E.coli_RNA_Quantitation_Kit_2G.jpg?v=1742293356"},{"product_id":"mycobacteria-dna-extraction-kit-mycoshentek™","title":"Mycobacteria DNA Extraction Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eApproximately 50 minutes\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature or 2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycobacteria DNA Extraction Kit is designed for extraction of trace Mycobacteria DNA from a variety of biologic sample types. The kit supports direct extraction from sample volumes ≤400 μL or concentration of larger volumes by centrifugation prior to extraction. It is compatible with manual magnetic-particle based workflows and automated extraction using the rHCDpurify\/rDNApurify instrument. The kit workflow includes pretreatment, Proteinase K digestion, magnetic particle binding (with isopropanol), two wash steps (ethanol-based), and heat elution to produce PCR-ready DNA (minimum recommended eluate ≥40 μL). The kit is supplied for research use only and is intended for downstream molecular detection (e.g., real-time PCR) with MycoSHENTEK detection reagents or other PCR systems.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eProduct No.: 1503601; reagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eExtraction of Mycobacteria DNA from cell-free and cell culture samples for downstream molecular detection (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003eRecommended ≤400 μL per sample; if \u0026gt;400 μL concentrate to ≈400 μL by centrifugation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K\u003c\/td\u003e\r\n \u003ctd\u003eAdd 20 μL Proteinase K per sample during digestion\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLysis\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100 μL Lysis buffer; incubate at 55 °C for 60 min (digestion)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding\u003c\/td\u003e\r\n \u003ctd\u003eAdd 200 μL Binding solution + 200 μL isopropanol + 50 μL Magnetic particles per sample; shake 5 min for binding\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWashes\u003c\/td\u003e\r\n \u003ctd\u003eWash 1: 700 μL Wash buffer A (ethanol added before use); Wash 2: 700 μL Wash buffer B (70% ethanol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 50 μL Elution buffer; incubate at 70 °C for 7 min; recover at least 40 μL eluate\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomated run time\u003c\/td\u003e\r\n \u003ctd\u003erHCDpurify \/ rDNApurify program: ~50 minutes (plate-based automated run)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCentrifugation speeds \/ times\u003c\/td\u003e\r\n \u003ctd\u003eCell-free sample concentration: 16000×g for 15 min; Cell pelleting: 72×g for 5 min; Debris precipitation: 450×g for 10–15 min; brief spins (3 s) used frequently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particle separation time\u003c\/td\u003e\r\n \u003ctd\u003eSeparation typically 3–5 min\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAir-dry time (post-wash)\u003c\/td\u003e\r\n \u003ctd\u003e30 s to 3 min depending on environment; avoid over-drying\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components storable under indicated conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTrace DNA extraction:\u003c\/strong\u003e Optimized to extract low-abundance (trace) Mycobacteria DNA from various biologic matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic bead-based purification:\u003c\/strong\u003e Magnetic particle chemistry provides efficient capture and wash-based purification compatible with manual and automated workflows.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eManual and automated workflows:\u003c\/strong\u003e Validated for manual tube-based extraction and automated plate-based extraction using rHCDpurify \/ rDNApurify instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePCR-ready eluate:\u003c\/strong\u003e Produces a concentrated eluate (recommended ≥40 μL) suitable for downstream real-time PCR detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eRoom-stable major reagents:\u003c\/strong\u003e Most reagents are stored at room temperature; selected reagents (e.g., cell lysis\/pretreatment\/magnetic particles) refrigerated as indicated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow reagents included:\u003c\/strong\u003e Includes lysis, binding, wash, elution buffers, proteinase K, magnetic particles and pretreatment reagents for sample types.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell-free biologics (e.g., serum, plasma, cell-free supernatants)\u003c\/li\u003e\r\n \u003cli\u003eExtraction of Mycobacteria DNA from cell culture samples and cell pellets\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for downstream molecular assays including real-time PCR\/qPCR\u003c\/li\u003e\r\n \u003cli\u003eResearch applications and in-process quality control testing of biologics (Research Use Only)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 10 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) — add 20 mL anhydrous ethanol before use\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 15 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature; 5 mL × 1 bottle\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Cell lysis buffer (NND039)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e5M NaCl (NND040)\u003c\/span\u003e\u003cstrong\u003eFollow label; 500 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePretreatment buffer (NND041)\u003c\/span\u003e\u003cstrong\u003e2–8 °C; 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND034)\u003c\/span\u003e\u003cstrong\u003eFollow label (equilibrate to room temp before use); 1.25 mL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND024)\u003c\/span\u003e\u003cstrong\u003eFollow label; 1 mL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle-based DNA extraction (manual or automated)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Read Material Safety Data Sheets (MSDS) and use appropriate PPE (gloves, lab coat, eye protection, mask).\u003c\/p\u003e\r\n \u003cp\u003e• Use separate, dedicated laboratory areas and equipment for reagent preparation, sample preparation, amplification, and post-amplification to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure ambient temperature ≥22 °C before starting experiments when recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Balance and vortex magnetic particles before use; centrifuge tubes briefly (3 s) when instructed to collect liquid from caps\/walls.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Wash buffer A by adding 20 mL anhydrous ethanol before first use; prepare Wash buffer B as 70% ethanol separately.\u003c\/p\u003e\r\n \u003cp\u003e• Do not over-dry the magnetic particle pellet after ethanol wash; over-drying may make resuspension difficult.\u003c\/p\u003e\r\n \u003cp\u003e• Perform mycobacterial DNA detection assays the same day as extraction to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• For automated runs: perform UV sterilization of the instrument for at least 15 min and clean internal walls with 75% alcohol wipes before and after runs; minimum interval between extractions is 30 min.\u003c\/p\u003e\r\n \u003cp\u003e• After automated extraction, immediately transfer eluate to a new tube and ensure ≥40 μL is recovered for downstream assays.\u003c\/p\u003e\r\n \u003cp\u003e• Dispose of used tips and liquid waste according to laboratory biohazard and local regulations.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503601.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated for cell-free samples (e.g., serum, plasma, supernatants) and cell culture samples (cell pellets\/resuspensions). Samples ≤400 μL can be processed directly; larger volumes should be concentrated to ≈400 μL before extraction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended elution volume and expected yield for downstream PCR?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandard elution uses 50 μL Elution buffer with incubation at 70 °C for 7 minutes. Recover at least 40 μL eluate for downstream PCR\/qPCR applications.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can I run the kit on an automated instrument?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with rHCDpurify \/ rDNApurify automated plate-based instruments. The automated program run time is approximately 50 minutes for a typical plate protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should magnetic particles be handled before use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eEquilibrate magnetic particles to room temperature for 10 minutes and vortex thoroughly to fully resuspend particles before aliquoting to samples to ensure consistent binding performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO) — For research purposes; not for diagnostic use\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45189176230069,"sku":"1503601","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Extraction_Kit.jpg?v=1742292295"},{"product_id":"mycobacteria-dna-detection-kit-mycoshentek™","title":"Mycobacteria DNA Detection Kit MycoSHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eMycoSHENTEK® Mycobacteria DNA Detection Kit is used, together with MycoSHENTEK® Mycobacteria DNA Extraction Kit, to qualitatively determine whether there is Mycobacteria contamination in cells, cell culture-derived biologicals, and similar materials using real-time qPCR technology. The integrated system has been validated with a limit of detection of 10–100 CFU\/mL and can detect more than 100 different Mycobacteria species. The assay chemistry includes dUTP and is compatible with UNG (uracil-N-glycosylase) treatment to prevent carry-over contamination. An Internal Control (IC) is provided to monitor PCR inhibition and\/or extraction efficiency. The kit is supplied with positive and negative controls and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd\u003e1503602\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit Format \/ Reactions\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 Reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQualitative detection of Mycobacteria contamination in cells and cell culture-derived biologicals\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Detection (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated at 10–100 CFU\/mL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\r\n \u003ctd\u003eValidated to detect \u0026gt;100 Mycobacteria species\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay type\u003c\/td\u003e\r\n \u003ctd\u003eReal-time qPCR (probe-based)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL (10 µL qPCR MIX + 20 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eMB qPCR Reaction Buffer 8 µL; MB Primer \u0026amp; Probe MIX 1.5 µL; Internal Control (IC) 0.5 µL; total 10 µL. UNG optional (0.1 U per reaction).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG 25 °C 10:00 (1 cycle); Activation 95 °C 10:00 (1 cycle); 45 cycles of Denaturation 95 °C 00:15, Annealing 60 °C 00:30, Extension 72 °C 01:30 (read fluorescence during extension).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls\u003c\/td\u003e\r\n \u003ctd\u003ePositive Control (PC), No Template Control (NTC), Negative Control Sample (NCS), Internal Control (IC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\r\n \u003ctd\u003eTypical interpretation: FAM positive cutoff Ct \u0026lt;40.00 (positive if at least one well Ct\u0026lt;40 and normal amplification); VIC (IC) expected Ct \u0026lt;35.00 for normal amplification; PC Ct \u0026lt;35.00. NTC FAM Ct ≥40.00 or no amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, and other real-time PCR systems supporting probe detection\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see individual component storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Validated limit of detection down to 10–100 CFU\/mL for broad Mycobacteria detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed and validated to detect over 100 different Mycobacteria species.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarry-over contamination control:\u003c\/strong\u003e Chemistry includes dUTP and is compatible with UNG treatment to prevent and eliminate carry-over contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IC):\u003c\/strong\u003e IC monitors PCR inhibition and\/or extraction efficiency; can be added pre-extraction or to the PCR mix.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-validated workflow:\u003c\/strong\u003e Includes positive and negative controls and recommended plate layouts and acceptance criteria for straightforward result interpretation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Works with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQualitative detection of Mycobacteria contamination in cell lines\u003c\/li\u003e\r\n \u003cli\u003eScreening of cell culture-derived biologicals for Mycobacteria contamination\u003c\/li\u003e\r\n \u003cli\u003eRoutine mycoplasma\/contaminant monitoring workflows in research laboratories (Mycobacteria-specific)\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of Mycobacteria DNA\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB qPCR Reaction Buffer (NNB011)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light; 400 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Primer \u0026amp; Probe MIX (NNC035)\u003c\/span\u003e\u003cstrong\u003e2–8 °C until use or -20 °C; 75 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Internal Control (IC) (NNA037)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or -20 °C; 600 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMB Positive Control (PC) (NNA041)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 500 µL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C or room temperature per label; 1.5 mL × 1\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: use UV decontamination (30 minutes) and 75% ethanol to clean surfaces and equipment before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use UNG enzyme optionally to reduce carry-over contamination (check effective amount before use).\u003c\/p\u003e\r\n \u003cp\u003e• If VIC (IC) signal is inhibited, repeat sample preparation or remove inhibitors and repeat the assay.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout and include PC, NTC and NCS as described; use duplicates for samples and controls.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1503602.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe integrated system has been validated with a limit of detection of approximately 10–100 CFU\/mL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the kit detect multiple Mycobacteria species?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The assay has been validated to detect more than 100 different Mycobacteria species.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Is an Internal Control provided and how should it be used?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAn Internal Control (IC) is provided. It can be added during sample extraction to monitor extraction efficiency or added to the PCR mix to monitor PCR inhibition. If IC was added during extraction, replace IC volume with DNA dilution buffer when preparing the qPCR mix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the recommended instruments and reaction setup?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, CFX96, 7500). Prepare 30 µL reaction volumes (10 µL qPCR MIX + 20 µL sample\/control) and run the recommended cycling program with UNG treatment if used.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eFor Research Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"Default Title","offer_id":43795617874101,"sku":"1503602","price":1525.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycobacteria_DNA_Detection_Kit.jpg?v=1742292467"},{"product_id":"mycoplasma-dna-extraction-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Extraction Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRoom temperature \/ 2–8 °C \/ -20 °C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Extraction Kit (2G) is a magnetic particle–based DNA extraction kit designed for isolation of trace mycoplasma DNA from master cell banks, working cell banks, virus seed banks and complex sample matrices (e.g., up to 10^7 cells culture, 5% human albumin solutions, high-concentration plasmids). The kit is supplied for 50 extractions and is intended to be used in an integrated workflow together with the MycoSHENTEK® Mycoplasma Detection Kit (2G). The method is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection. The kit supports manual extraction and automated extraction using the rHCDpurify system. The validated detection limit is 10 CFU\/mL. The protocol includes sample pretreatment, enzymatic digestion (Proteinase K), magnetic-particle binding, wash steps, and elution to produce purified DNA suitable for downstream real-time PCR assays.\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended use \/ scope\u003c\/td\u003e\n \u003ctd\u003eExtraction of trace mycoplasma DNA from cell banks, virus seed banks and complex matrices (cell cultures, 5% human albumin, high-concentration plasmids).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidated standards\u003c\/td\u003e\n \u003ctd\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection limit (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated when used with MycoSHENTEK® Mycoplasma Detection Kit (2G)).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSample input volume\u003c\/td\u003e\n \u003ctd\u003eUp to ~400 µL directly; larger volumes should be concentrated by centrifugation and reduced to ~400 µL prior to extraction.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 50 extractions.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTypical digestion\/incubation conditions\u003c\/td\u003e\n \u003ctd\u003eAdd 20 µL Proteinase K and 100 µL Lysis buffer; incubate 55°C for 60 min for most matrices; for 5% human albumin incubate at 25°C for 60 min.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eBinding step\u003c\/td\u003e\n \u003ctd\u003ePer sample add 200 µL Binding Buffer, 200 µL isopropanol and 30 µL Magnetic particles; vertical mixing ~5 min to bind nucleic acids.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eWash steps\u003c\/td\u003e\n \u003ctd\u003eTwo washes: 700 µL Wash buffer A then 700 µL Wash buffer B; remove residual ethanol and air-dry magnetic pellet briefly (30 s–3 min).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eElution\u003c\/td\u003e\n \u003ctd\u003eElute with 50 µL Elution buffer; incubate at 70°C for 7 min with intermittent vortexing; recover eluate (~40 µL+ recommended for downstream assay).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAutomated run time (rHCDpurify)\u003c\/td\u003e\n \u003ctd\u003eApprox. 43 minutes per plate run (program: Myco-2G).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage stability\u003c\/td\u003e\n \u003ctd\u003eKit components stable up to 24 months when stored under recommended conditions (see component list).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eCritical centrifugation speeds\u003c\/td\u003e\n \u003ctd\u003eDebris removal: 7,250×g (10 s); concentration and plasmid\/cell concentration steps: 18,000×g (30 min); microcentrifuge brief spins: 3–10 s for liquid collection.\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls\u003c\/td\u003e\n \u003ctd\u003eNegative control sample (NCS) procedure provided; Internal Control (IC) (2G) is used during extraction (IC is supplied in the companion detection kit).\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated detection limit of 10 CFU\/mL when used with MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad matrix compatibility:\u003c\/strong\u003e Works with cell cultures (up to 10^7 cells), cell culture supernatants, 5% human albumin solutions, high-concentration plasmids and other complex matrices.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports both manual magnetic-particle extraction and automated extraction on the rHCDpurify system.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePharmacopeial validation:\u003c\/strong\u003e Validated in accordance with USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection workflows.\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eComplete extraction chemistry:\u003c\/strong\u003e Includes lysis, binding, wash and elution reagents plus magnetic particles and proteinase K for robust nucleic acid recovery from low-copy targets.\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eMycoplasma testing of master cell banks (MCBs) and working cell banks (WCBs)\u003c\/li\u003e\n \u003cli\u003eMycoplasma testing of virus seed banks\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture samples (up to 10^7 cells)\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture supernatants\u003c\/li\u003e\n \u003cli\u003eTesting of samples containing 5% human albumin\u003c\/li\u003e\n \u003cli\u003eExtraction from high-concentration plasmid preparations prior to mycoplasma detection\u003c\/li\u003e\n \u003cli\u003eSample preparation prior to real-time PCR-based mycoplasma detection\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (after adding 20 mL anhydrous ethanol prior to first use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eLysis buffer (NND028)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (heat at 37°C if cloudy)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePretreatment buffer (NND002) — 1.25 mL × 4 tubes\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMagnetic particles (NND033) — 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2–8 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003e5 M NaCl (NND040) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution I (NND003) — 25 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRoom temperature (dilute 1:99 in DNA Dilution Buffer prior to use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePrecipitation solution II (NND004) — 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eProteinase K (NND023) — 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C \/ according to label (keep frozen until use)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based DNA extraction (magnetic bead chemistry) for downstream qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• Read MSDS and use appropriate PPE (eyewear, mask, gloves, lab coat).\u003c\/p\u003e\n \u003cp\u003e• Divide lab into separate areas (negative\/preparation, positive\/sample prep, amplification) and use dedicated equipment to prevent cross-contamination.\u003c\/p\u003e\n \u003cp\u003e• Ensure ambient temperature is not lower than 22°C before starting experiments (per manual recommendation).\u003c\/p\u003e\n \u003cp\u003e• Centrifuge tubes before opening to avoid splashing; handle tubes carefully to prevent contamination.\u003c\/p\u003e\n \u003cp\u003e• Change gloves frequently and use different gloves\/lab coats in separate areas.\u003c\/p\u003e\n \u003cp\u003e• Dispose of used tips and liquid waste according to local regulations; do not open post-PCR products in amplification area.\u003c\/p\u003e\n \u003cp\u003e• Avoid over-drying magnetic particle pellet during ethanol removal to prevent incomplete elution.\u003c\/p\u003e\n \u003cp\u003e• Perform DNA detection the same day as extraction to ensure accuracy.\u003c\/p\u003e\n \u003cp\u003e• For automated runs on rHCDpurify: UV sterilize the instrument for ≥15 minutes and clean inner walls with 75% ethanol wipes before and after runs; minimum interval between extractions should be 30 minutes.\u003c\/p\u003e\n \u003cp\u003e• When removing supernatants, avoid aspirating magnetic particles; use magnetic stand and allow full particle separation (3–5 min) before removing supernatant.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509840.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection when this extraction kit is used with the MycoSHENTEK® Mycoplasma Detection Kit (2G)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe workflow is validated with a detection limit of 10 CFU\/mL when used with the MycoSHENTEK® Mycoplasma Detection Kit (2G).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can I process sample volumes larger than 400 µL?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. For sample volumes \u0026gt;400 µL the protocol requires concentration by centrifugation to a final volume of approximately 400 µL before extraction.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which samples require a different digestion temperature?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eMost sample types are incubated with Proteinase K at 55°C for 60 minutes. Samples containing 5% human albumin and their controls should be incubated at 25°C for 60 minutes.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the kit compatible with automation?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with automated extraction using the rHCDpurify system (example program Myco-2G, ~43 minutes runtime).\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO); validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII for mycoplasma detection\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45191334133941,"sku":"1509840","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Extraction_Kit_2G.jpg?v=1742362510"},{"product_id":"mycoplasma-dna-detection-kit-2g-mycoshentek™","title":"MycoSHENTEK™ Mycoplasma DNA Detection Kit (2G)","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n 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center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\n\u003cdiv class=\"shentek-wrapper\"\u003e\n \u003cdiv class=\"shentek-header-bar\"\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-header-item\"\u003e\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n \u003cdiv class=\"shentek-header-value\"\u003e-20°C (stable for 24 months)\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\n \u003ch2 class=\"shentek-title\"\u003e\n \n Product Description\n \u003c\/h2\u003e\n \u003cp class=\"shentek-text\"\u003eMycoSHENTEK® Mycoplasma DNA Detection Kit (2G) is a qualitative qPCR-based assay for detection of Mycoplasma, Spiroplasma and Acholeplasma contamination in master cell banks, working cell banks, virus seed stocks and cell culture–derived products. The kit is validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII, with a stated limit of detection of 10 CFU\/mL. The assay detects \u0026gt;200 species with high specificity and includes Internal Control (IC, 2G) to monitor PCR inhibition and optionally to evaluate extraction efficiency when added prior to extraction. The kit is supplied for 50 reactions and is intended for research use only (RUO).\u003c\/p\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\n \u003ctable class=\"shentek-table\"\u003e\n \u003cthead\u003e\n \u003ctr\u003e\n \u003cth\u003eParameter\u003c\/th\u003e\n \u003cth\u003eDetails\u003c\/th\u003e\n \u003c\/tr\u003e\n \u003c\/thead\u003e\n \u003ctbody\u003e\n \u003ctr\u003e\n \u003ctd\u003eAssay type\u003c\/td\u003e\n \u003ctd\u003eQualitative real-time PCR (qPCR) assay\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eLimit of detection (LOD)\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e10 CFU\/mL (validated)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eValidation \/ Standards\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eValidated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eSpecies coverage\u003c\/td\u003e\n \u003ctd\u003eDetects \u0026gt;200 species of Mycoplasma, Spiroplasma and Acholeplasma\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eControls included\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003eInternal Control (IC) (2G), Positive Control (PC) (2G)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReaction format \u0026amp; volume\u003c\/td\u003e\n \u003ctd\u003e30 μL total reaction volume: 10 μL qPCR MIX + 20 μL sample (per well)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eqPCR Mix composition (per reaction)\u003c\/td\u003e\n \u003ctd\u003eMy qPCR Reaction Buffer (2G) 8 μL; My Primer \u0026amp; Probe MIX (2G) 1.5 μL; Internal Control (IC) (2G) 0.5 μL (total 10 μL)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\n \u003ctd\u003eUNG 25°C 10:00 (1); Activation 95°C 10:00 (1); 45 cycles of: Denaturation 95°C 00:15, Annealing 62°C 00:30, Extension 72°C 01:30 (fluorescence read during extension)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eDetection channels \/ dyes\u003c\/td\u003e\n \u003ctd\u003eTarget: FAM; Internal Control: VIC; ROX optional as passive reference\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\n \u003ctd\u003eSHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500, Roche LightCycler 480 and other real-time PCR systems\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eIntended sample types \/ matrices\u003c\/td\u003e\n \u003ctd\u003eMaster cell bank, working cell bank, virus seed stock, cell culture-derived products and similar matrices (validated across a variety of matrices)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\n \u003ctd class=\"shentek-text-danger\"\u003e50 reactions\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\n \u003ctd\u003e-20°C; kit components stable up to 24 months (check label expiration date)\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eReconstitution instructions (controls)\u003c\/td\u003e\n \u003ctd\u003eReconstitute lyophilized IC (2G) in 600 μL DNA Dilution Buffer; reconstitute lyophilized PC (2G) in 500 μL DNA Dilution Buffer\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003ctr\u003e\n \u003ctd\u003eAcceptance criteria (Ct thresholds)\u003c\/td\u003e\n \u003ctd\u003eNTC: FAM Ct ≥ 40 or no amplification; VIC Ct \u0026lt; 35; PC: FAM Ct \u0026lt; 35; Unknown positive: FAM Ct \u0026lt; 40 (≥1 well) with normal amplification; If VIC inhibited, repeat extraction or remove inhibitors\u003c\/td\u003e\n \u003c\/tr\u003e\n \u003c\/tbody\u003e\n \u003c\/table\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003e\n\u003cstrong\u003eHigh analytical sensitivity:\u003c\/strong\u003e Validated LOD of 10 CFU\/mL allowing detection of low-level mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eBroad species coverage:\u003c\/strong\u003e Designed to detect more than 200 Mycoplasma, Spiroplasma and Acholeplasma species with high specificity\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eInternal control for inhibition and extraction monitoring:\u003c\/strong\u003e Internal Control (IC, 2G) can be added to PCR or to the sample prior to extraction to monitor PCR inhibition and evaluate extraction efficiency\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eValidated to pharmacopeia standards:\u003c\/strong\u003e Validated according to USP \u0026lt;63\u0026gt;, EP 2.6.7 and JP XVIII\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Provided My Primer \u0026amp; Probe MIX (2G) simplifies assay setup; lyophilized positive and internal controls included\u003c\/li\u003e\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (SHENTEK-96S, CFX96, 7500, LightCycler 480, etc.)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n \u003cul class=\"shentek-list\"\u003e\n \u003cli\u003eQualitative mycoplasma testing of master cell banks and working cell banks\u003c\/li\u003e\n \u003cli\u003eScreening of virus seed stocks for mycoplasma contamination\u003c\/li\u003e\n \u003cli\u003eTesting of cell culture–derived products and biologics for mycoplasma\u003c\/li\u003e\n \u003cli\u003eRoutine cell culture contamination monitoring and QC in R\u0026amp;D and production (research use only)\u003c\/li\u003e\n \u003c\/ul\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box\"\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eInternal Control (IC) (2G) (Part No. NNA035) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 600 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003ePositive Control (PC) (2G) (Part No. NNA039) - lyophilized\u003c\/span\u003e\u003cstrong\u003e-20°C (reconstitute in 500 μL DNA Dilution Buffer)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy qPCR Reaction Buffer (2G) (Part No. NNB004) 400 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C, protect from light\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eMy Primer \u0026amp; Probe MIX (2G) (Part No. NNC065) 75 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20°C\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-row\"\u003e\n \u003cspan\u003eUser Guide \/ Instructions for use\u003c\/span\u003e\u003cstrong\u003eRoom temperature (follow handling instructions)\u003c\/strong\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 Reactions | Method: qPCR\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv\u003e\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n \u003cp\u003e• For research use only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\n \u003cp\u003e• Work in a clean area: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before use.\u003c\/p\u003e\n \u003cp\u003e• Thaw kit components at 2–8°C or on ice; vortex and centrifuge briefly before use.\u003c\/p\u003e\n \u003cp\u003e• Protect My qPCR Reaction Buffer from light during storage and use.\u003c\/p\u003e\n \u003cp\u003e• If Internal Control (IC) was added during extraction, substitute equal volume of DNA Dilution Buffer in qPCR mix instead of IC.\u003c\/p\u003e\n \u003cp\u003e• Follow recommended plate layout and include controls (PC, NTC, NCS) in duplicate.\u003c\/p\u003e\n \u003cp\u003e• If VIC (IC) signal is inhibited (Ct ≥ threshold), repeat extraction or remove inhibitors and repeat assay.\u003c\/p\u003e\n \u003cp\u003e• Dispose of biological and chemical waste according to local regulations.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-card\"\u003e\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\n \u003c\/h3\u003e\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1509841.pdf\" target=\"_blank\"\u003e\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\n \u003c\/a\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003csection class=\"shentek-section\"\u003e\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n \u003cdiv class=\"shentek-faq\"\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) of the kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is validated with a limit of detection of 10 CFU\/mL.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Can the Internal Control (IC) be added before extraction?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. IC (2G) may be added to the sample prior to extraction to evaluate extraction efficiency; if IC is added during extraction, omit IC when preparing the qPCR mix and add equal volume of DNA Dilution Buffer instead.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: Which qPCR instruments are compatible with this kit?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, Bio-Rad CFX96, Applied Biosystems 7500 and Roche LightCycler 480. Use instrument software to set FAM for target and VIC for IC.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-item\"\u003e\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How are results interpreted (Ct thresholds)?\u003c\/div\u003e\n \u003cdiv class=\"shentek-faq-answer\"\u003eAcceptance criteria: Positive control (PC) FAM Ct \u0026lt; 35; NTC FAM Ct ≥ 40 or no amplification; Unknown sample: FAM Ct \u0026lt; 40 (at least one well) with normal amplification = Positive. If IC (VIC) is inhibited (VIC Ct ≥ threshold or absent), repeat extraction or remove inhibitors and retest.\u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/section\u003e\n\n \u003cdiv class=\"shentek-notice\"\u003e\n \u003cdiv class=\"shentek-notice-header\"\u003e\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-body\"\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003cdiv class=\"shentek-notice-item\"\u003e\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\n official compliance testing.\u003c\/p\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n \u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Reactions","offer_id":45191337345205,"sku":"1509841","price":1751.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycoSHENTEK_Mycoplasma_DNA_Detection_Kit_2G.jpg?v=1742362689"},{"product_id":"cf-dna-extraction-kits-new-technology","title":"Human Urine cfDNA Extraction Kit (50 Preps, 50 mL Input Capacity)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp data-end=\"516\" data-start=\"154\"\u003eThe Human Urine cfDNA Extraction Kit (Cat. CF-DNA-301) is designed for high-efficiency isolation of circulating cell-free DNA (cfDNA) from up to 50 mL of urine in a single preparation. The kit utilizes an innovative double-layer column technology that maximizes cfDNA recovery while eliminating genomic DNA contamination from lysed white blood cells.\u003c\/p\u003e\n\u003cp data-end=\"703\" data-start=\"518\"\u003eThe eluted cfDNA is highly pure and immediately ready for downstream molecular applications, including next-generation sequencing (NGS), qPCR, digital PCR, and methylation analysis.\u003c\/p\u003e\n\u003cp data-end=\"880\" data-start=\"705\"\u003eThis kit is particularly suited for applications requiring large input volumes, such as early-stage cancer detection or liquid biopsy studies where cfDNA abundance is low.\u003c\/p\u003e\n\u003ch2 data-end=\"880\" data-start=\"705\"\u003ePerformance Specifications\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eParameter    \u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 29px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003eSample volume    \u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003eUp to 50 mL urine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ecfDNA fragment size    \u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e80–500 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003eRecovery rate    \u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; height: 18px;\"\u003e≥90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eYield\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u0026gt;20 μg total cfDNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eDirect-use for NGS \/ PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eGenomic DNA contamination    \u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eNone detectable (WBC gDNA retained on membrane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eFormat    \u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e50 Preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2 data-end=\"157\" data-start=\"137\"\u003eApplications\u003c\/h2\u003e\n\u003cp data-end=\"222\" data-start=\"159\"\u003eThis high-capacity urine cfDNA extraction kit is optimized for:\u003c\/p\u003e\n\u003cul data-end=\"695\" data-start=\"224\"\u003e\n\u003cli data-end=\"357\" data-start=\"224\"\u003e\n\u003cp data-end=\"357\" data-start=\"226\"\u003eEarly cancer detection \u0026amp; minimal residual disease (MRD)\u003cbr data-end=\"288\" data-start=\"285\"\u003e(large input volume improves sensitivity for low-abundance markers)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"411\" data-start=\"359\"\u003e\n\u003cp data-end=\"411\" data-start=\"361\"\u003eLiquid biopsy \u0026amp; non-invasive genomic profiling\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"449\" data-start=\"413\"\u003e\n\u003cp data-end=\"449\" data-start=\"415\"\u003eUrothelial carcinoma screening\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"504\" data-start=\"451\"\u003e\n\u003cp data-end=\"504\" data-start=\"453\"\u003ePrenatal screening (urine-based cfDNA research)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"543\" data-start=\"506\"\u003e\n\u003cp data-end=\"543\" data-start=\"508\"\u003eTransplant rejection monitoring\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"598\" data-start=\"545\"\u003e\n\u003cp data-end=\"598\" data-start=\"547\"\u003eInfectious disease monitoring via urinary cfDNA\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"639\" data-start=\"600\"\u003e\n\u003cp data-end=\"639\" data-start=\"602\"\u003eEpigenetic and methylation assays\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli data-end=\"695\" data-start=\"641\"\u003e\n\u003cp data-end=\"695\" data-start=\"643\"\u003eBiomarker discovery from low-input urine samples\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-end=\"908\" data-start=\"697\"\u003eLarge-volume capability significantly enhances assay sensitivity, making the \u003cstrong data-end=\"788\" data-start=\"774\"\u003eCF-DNA-301\u003c\/strong\u003e especially suitable for early-stage or low-abundance cfDNA studies where typical 1–5 mL plasma inputs are insufficient.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSample Volumes\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%; height: 97px;\" class=\"tableizer-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5996px;\" class=\"tableizer-firstrow\"\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eKit Variant\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eSample Volume \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#301\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 50ml of Urine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#302\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 5ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#303\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 0.7ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at RT (20-25C). \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/human-urine-cf-dna-extraction-kit-50ml-50-units-302?variant=44624546005173\"\u003e\u003cspan\u003e(Click Here) #302 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/human-plasma-cf-dna-extraction-kit-mini-0-7ml-50-units-303?variant=44624549216437\"\u003e\u003cspan\u003e(Click Here) #303 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/cfDNA_301.pdf?v=1769460033\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cmeta charset=\"utf-8\"\u003eThis product is intended for research use only.\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"Default Title","offer_id":44624534175925,"sku":"CF-DNA-301","price":2009.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/301_kit.jpg?v=1732874816"},{"product_id":"human-urine-cf-dna-extraction-kit-50ml-50-units-302","title":"Human Plasma cf-DNA Extraction Kit, Midi (50 Preps, 5 mL Input Capacity)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe cf-DNA Extraction Kits use a double-layer column to extract circulating cell-free DNA from either plasma or human urine. The eluted nucleic acid can be directly used for downstream applications, such as NGS and PCR.\u003c\/p\u003e\n\u003cp\u003eThis product is intended to detect early cancer diagnosis.\u003c\/p\u003e\n\u003ch2\u003ePerformance Specifications\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ecf-DNA size\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e80–500 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ecf-DNA recovery rate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e≥90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eCapability\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u0026gt;12 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eDirect-use for NGS \/ PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eGenomic DNA contamination\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eNone detectable (WBC gDNA retained on membrane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e50 Preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSample Volumes\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable class=\"tableizer-table\" style=\"border-collapse: collapse; width: 90%; height: 97px;\"\u003e\n\u003cthead\u003e\n\u003ctr class=\"tableizer-firstrow\" style=\"height: 19.5996px;\"\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eKit Variant\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eSample Volume \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#301\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 50ml of Urine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#302\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 5ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#303\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 0.7ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at RT (20-25C). \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/cf-dna-extraction-kits-new-technology?variant=44624534175925\"\u003e\u003cspan\u003e(Click Here) #301 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/human-plasma-cf-dna-extraction-kit-mini-0-7ml-50-units-303?variant=44624549216437\"\u003e\u003cspan\u003e(Click Here) #303 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/cfDNA_302.pdf?v=1769460033\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is for research use only.\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"Default Title","offer_id":44624546005173,"sku":"CF-DNA-302","price":1700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/302_kit.jpg?v=1732874810"},{"product_id":"human-plasma-cf-dna-extraction-kit-mini-0-7ml-50-units-303","title":"Human Plasma cf-DNA Extraction Kits, Mini (50 Preps, 0.7 mL Input Capacity)","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003eThe cf-DNA Extraction Kits use a double-layer column to extract circulating cell-free DNA from either plasma or human urine. The eluted nucleic acid can be directly used for downstream applications, such as NGS and PCR.\u003c\/p\u003e\n\u003cp\u003eThis product is intended for prenatal cancer diagnosis.\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePerformance Specifications\u003cbr\u003e\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ecf-DNA size\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e80–500 bp\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ecf-DNA recovery rate\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e≥90%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eCapability\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e\u0026gt;12 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eDirect-use for NGS \/ PCR\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eGenomic DNA contamination\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eNone detectable (WBC gDNA retained on membrane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center;\"\u003e50 Preps\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eSample Volumes\u003c\/span\u003e\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%; height: 97px;\" class=\"tableizer-table\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19.5996px;\" class=\"tableizer-firstrow\"\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eKit Variant\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 19px; border-style: solid; text-align: center;\"\u003e\u003cstrong\u003eSample Volume \u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#301\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 50ml of Urine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#302\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 5ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 26.6016px;\"\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003e#303\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 26px; border-style: solid; text-align: center;\"\u003eUp to 0.7ml of Plasma\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at RT (20-25C). \u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eRelated Products\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/cf-dna-extraction-kits-new-technology?variant=44624534175925\"\u003e\u003cspan\u003e(Click Here) #301 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/human-urine-cf-dna-extraction-kit-50ml-50-units-302?variant=44624546005173\"\u003e\u003cspan\u003e(Click Here) #302 cf-DNA Extraction Kit\u003c\/span\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eProduct Manual\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/cfDNA_303.pdf?v=1769460027\"\u003eUser Manual\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eThis product is for research use only.\u003c\/p\u003e","brand":"Genetic Tools","offers":[{"title":"Default Title","offer_id":44624549216437,"sku":"CF-DNA-303","price":1597.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/303_kit.jpg?v=1732874813"},{"product_id":"rnasimple-total-rna-kit-dp419-50-preps-tiangen","title":"RNAsimple Total RNA Kit For the High-efficient Total RNA Extraction Using The Widely Used Centrifugal Column (50 preps)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate\/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.\u003c\/p\u003e\n\u003cp\u003eThis kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5×106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.\u003c\/p\u003e\n\u003ch3\u003eStorage\u003c\/h3\u003e\n\u003cp\u003eBuffer RZ should be stored protected from light at 2-8°C for 15 months;\u003cbr\u003eothers stored at room temperature (15-30°C) for 15 months.\u003c\/p\u003e\n\u003ch3\u003e\n\u003cspan\u003e\u003c\/span\u003eRequired Reagents\u003c\/h3\u003e\n\u003cp\u003e\u003cspan\u003eChloroform, Ethanol (96% -100%)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003eThe high-purity ready-to-use RNA is suitable for sensitive downstream applications.\u003c\/li\u003e\n\u003cli\u003eWide applications: The purified RNA can be applied to various experimental samples.\u003c\/li\u003e\n\u003cli\u003eThe experiment can be completed in 1 hour with simple operations.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eRT-PCR\u003c\/li\u003e\n\u003cli\u003eNorthern Blot, Dot Blot.\u003c\/li\u003e\n\u003cli\u003eReal-Time PCR\u003c\/li\u003e\n\u003cli\u003ePolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eExperimental Example\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg height=\"418\" width=\"418\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RNAsimple_Total_RNA_Kit_2_167d1a6d-946f-4ea7-bc75-1f8895d2ad7d.jpg?v=1732169203\" alt=\"\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg alt=\"Experimental Example\" src=\"https:\/\/www.tiangenbioem.com\/uploads\/dcbe1c629.png\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003eMaterial: 20 mg rat spleen tissue\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cbr\u003eMethod: The total RNA of rat spleen tissue was isolated using the RNAsimple Total RNA Kit.\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\n\u003cbr\u003eResults: Please see the above agarose gel electrophoresis picture. 2-4 μl of 100 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V\/cm for 30 min on a 1% agarose.\u003c\/div\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cbr\u003e\u003c\/div\u003e","brand":"Tiangen","offers":[{"title":"50 Preps","offer_id":44837810700469,"sku":"DP419","price":183.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RNAsimpleTotalRNAKit.jpg?v=1732881628"},{"product_id":"super-plant-genomic-dna-kit-biofargo","title":"Super Plant Genomic DNA Kit","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe Super Plant Genomic DNA Kit adopts a highly efficient, DNA-specific adsorption spin column and a unique buffer system, which can separate and purify high-quality genomic DNA from a variety of plant tissues. The unique precipitation solution can precipitate and remove the protein, polysaccharide, phenol and other impurities in the polysaccharides \u0026amp; polyphenolics-rich plant samples. The extracted genomic DNA has high purity, stable and reliable quality.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSimple and fast: Ultra-pure gDNA could be obtained within 1 hour.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWide applications: Suitable for various plant tissues, especially for polysaccharides \u0026amp; polyphenolics-rich plants.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNon-toxic: Safe operation because no need of phenol or chloroform for extraction.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh purity and efficiency: Ultra-pure DNA can be obtained efficiently, which can be applied directly in molecular biology experiments such as chip hybridization, NGS, etc.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eApplications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eSuitable for a variety of regular operations, including restriction enzyme digestion, PCR, library construction, chip hybridization and Southern blot.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer GA: 30 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer GHL: 20 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer GDZ: 45 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer PWD: 20 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProteinase K: 1 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMagAttract Suspension GH: 0.75 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eBuffer TB: 15 ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThis kit can be stored dry for 12 months under room temperature (15-25℃). For long-term storage, store at 2-8℃. When stored at 2-8℃, precipitation might be formed in buffer. Place the buffers at room temperature for a period of time before use, and if necessary, incubate the buffers in a 37 ℃ water bath for 10 minutes to dissolve the precipitation.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eCase Study\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Super-Plant-Genomic-DNA-Kit_600x600.jpg?v=1743139965\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\n\u003cp\u003eDNA was extracted from leaves of 100 mg Malus spectabilis and peach tree with Super Plant Genomic Kit and relevant kits of supplier A and Q according to the instructions. . The experimental results show that Super Plant Genomic Kit has higher DNA extraction yield.\u003c\/p\u003e\n\u003cp\u003e\u003cimg style=\"float: none;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/plant-genomic-DNA-extraction-Kit_600x600.jpg?v=1743140007\"\u003e\u003c\/p\u003e\n\u003cp\u003eDNA was extracted from laves of 100 mg various plant tissues with Super Plant Genomic Kit according to the instruction. The experimental results show that Super Plant Genomic Kit can be  used to extract a variety of complex plant samples,including polysaccharides \u0026amp; polyphenolics-rich plant samples, with high extraction yield and purity.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"Tiangen","offers":[{"title":"50 preps","offer_id":45018015662261,"sku":"GDP360","price":244.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Super_Plant_Genomic_DNA_Kit-Tiangen.jpg?v=1743140518"},{"product_id":"tianamp-genomic-dna-kit-biofargo","title":"TIANamp Genomic DNA Kit","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eThe TIANamp Genomic DNA Kit provides a fast and easy silica-based format for DNA purification. The kit simplifies the purification of DNA from a wide range of sample types, including animal blood (≤ 1 ml), cultured cells, and tissues commonly encountered in life science, veterinary, and genotyping applications. Most samples could be directly lysed with the provided buffer system, eliminating the need for mechanical disruption and also reducing hands-on time. The kit purifies high-quality DNA without proteins and inhibitors. The purified DNA is suitable for PCR, restriction digestion and Southern blot.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eRequired Reagents\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eRed Cell Lysis Buffer (for blood samples more than 200 μl, Cat# 4992810), ethanol, RNase A (optional)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSilica-membrane technology provides fast way to purify ready-to-use high quality gDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh DNA yield, excellent repeatability.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThis kit significantly removes contaminants and inhibitors, facilitating downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eImportant Notes\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePlease use fresh samples to ensure the integrity of the purified gDNA.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eIf the sample starting amount exceeds the recommended range of the kit, it is recommended to increase the amount of Buffer GA and Buffer GB, and split the column loading in separate times, or perform the experiment using two spin columns.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor long-term storage of the gDNA, it is recommended to store in buffer TE. Please ensure that the OD260\/OD280 value is in the range of 1.7-1.9, and the concentration of gDNA is 100-300 ng\/μl.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20210806163358_83018_c2107cda-3680-4c5a-970c-d4141b65bb6b_480x480.jpg?v=1736841716\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2 style=\"text-align: left;\"\u003e\u003cspan\u003eDNA Extraction Yield From Various Plant Tissues\u003c\/span\u003e\u003c\/h2\u003e\n\u003cdiv style=\"text-align: left;\"\u003e\u003cimg style=\"margin-bottom: 16px; float: none;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20200903112437_73931_480x480.jpg?v=1736841773\"\u003e\u003c\/div\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eRoom temperature (15-25℃)\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003ePublications\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eDual-responsive nanocarriers for efficient cytosolic protein delivery and CRISPR-Cas9 gene therapy of inflammatory skin disorders. \u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003ePublication: Science Advances \u003c\/span\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003egdp304, dp304,dp 304, gdp 304\u003c\/div\u003e","brand":"Tiangen","offers":[{"title":"200 preps","offer_id":45018048299189,"sku":"GDP304-03","price":380.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20210806163358_83018_b4a520e5-833d-49c9-9117-dc5ab51e52c5.jpg?v=1736841706"},{"product_id":"residual-vero-dna-quantitation-kit-shentek™","title":"Residual Vero DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Vero DNA Quantitation Kit is designed for quantitative measurement of residual Vero host cell DNA in biopharmaceutical samples across processing stages, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) with a Vero-specific primer \u0026amp; probe mix to deliver rapid, specific and reliable quantitation down to the femtogram (fg) level. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit provides reagents sufficient for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Total reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (Standard curve \/ Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at the femtogram (fg) level (document states femtogram-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST0 (3000 pg\/µL prepared stock); ST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL; ST6 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL; Sample\/Standard\/NTC\/NCS addition: 10 µL; Total reaction volume: 30 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + Vero Primer\u0026amp;Probe MIX 3 µL = 20 µL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetector \/ Dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: (none specified)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ threshold\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selected; Threshold for Vero-DNA set to 0.02; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ controls\u003c\/td\u003e\r\n \u003ctd\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard; ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycles\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments (examples)\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 (other real-time PCR systems likely compatible after instrument-specific setup)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed for quantitation down to the femtogram (fg) level for detection of trace residual Vero DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Includes qPCR Reaction Buffer and Vero-specific Primer \u0026amp; Probe MIX to simplify setup and standardization.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePredefined standard curve:\u003c\/strong\u003e Provided Vero DNA Control and recommended serial dilutions (ST1–ST6) for absolute quantitation and standard curve construction.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example instrument settings provided for common real-time PCR systems; parameters can be adjusted per instrument\/software.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eControl strategy and QC guidance:\u003c\/strong\u003e Includes instructions for NTC, NCS, and Extraction Reference Control (ERC) with recovery acceptance criteria (50%–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing during biologics manufacturing\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in Vero cell lines\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 µL × 2 tubes); protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX (NNC016)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 µL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for use in diagnostic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and maintain a DNA-free workspace (UV decontamination and 75% ethanol recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit components at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light during storage and handling.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare and run at least five concentrations on the standard curve; include NTC, NCS and ERC controls as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should be ≥ 35 cycles or undetermined; ERC recovery should be between 50% and 150%; NCS Ct must be greater than mean Ct of lowest standard or less than validated LOQ if LOQ \u0026lt; lowest standard.\u003c\/p\u003e\r\n \u003cp\u003e• Configure instrument-specific analysis parameters according to your instrument\/software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030204V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the kit sensitivity (LOD\/LOQ)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended to quantify residual Vero DNA down to the femtogram (fg) level. The validated limit of quantitation (LOQ) should be determined by laboratory validation; if LOQ is below the lowest standard, ensure NCS Ct is less than the LOQ concentration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents sufficient for 100 reactions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended storage conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C. The components are stable for up to 24 months; check individual tube labels for expiration.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which qPCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems. The user guide provides example settings for SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and LineGene 9600. For other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187363700917,"sku":"SK030204V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA_Quantitation_Kit.png?v=1742278593"},{"product_id":"residual-sv40-lta-e1a-dna-quantitation-kit-2g","title":"Residual SV40 LTA\/E1A DNA Quantitation Kit (2G) SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual SV40LTA \u0026amp; E1A DNA Quantitation Kit (2G) is a duplex real-time PCR (qPCR) kit designed to quantify residual SV40 large T antigen (LTA) and E1A host cell DNA (e.g., from HEK293T). The assay is duplex (simultaneous detection of SV40LTA and E1A) with an internal positive control (IPC) and achieves sensitivity to the level of 10^1 copies\/μL. The kit includes linear and non-linear DNA controls for standard curve generation and is compatible with common real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96). Reagents are provided for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual SV40LTA and E1A host cell DNA in biological samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eDuplex real-time PCR (qPCR) with target-specific primer\/probe mixes and IPC\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e10^1 copies\/μL (stated detection level)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 4.67 × 10^6 to 4.67 × 10^1 copies\/μL (ST1–ST6)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve dynamic range (non-linear DNA)\u003c\/td\u003e\r\n \u003ctd\u003eTypical standard curve points: 2.80 × 10^6 to 2.80 × 10^2 copies\/μL (ST1–ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl concentrations (as supplied)\u003c\/td\u003e\r\n \u003ctd\u003eLinear DNA Control: SV40LTA 4.67 × 10^9 copies\/μL; E1A 4.97 × 10^9 copies\/μL. Non-linear DNA Control: SV40LTA 2.80 × 10^9 copies\/μL; E1A 2.98 × 10^9 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation using standard curve (user-prepared serial dilutions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction setup \/ volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 μL (20 μL qPCR MIX + 10 μL sample or standard). qPCR MIX per reaction: qPCR Reaction Buffer 15.9 μL, Primer \u0026amp; Probe MIX 2.8 μL, IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15; Annealing\/extension 60 °C 1:00; 40 cycles. Fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other real-time PCR instruments supported with appropriate settings)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set Standard Curve (Absolute Quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within ±1.0 of the NCS Ct(IPC); significant increase indicates potential inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance thresholds\u003c\/td\u003e\r\n \u003ctd\u003eSV40LTA detection value of NTC should be ≤ 14.32 copies\/μL; E1A detection value of NTC should be ≤ 17.79 copies\/μL (or use user-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under recommended conditions for up to 24 months\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex real-time PCR:\u003c\/strong\u003e Simultaneous quantitation of SV40LTA and E1A targets in the same reaction using target-specific probes and an IPC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects down to 10^1 copies\/μL enabling detection of low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use controls and mixes:\u003c\/strong\u003e Includes linear and non-linear DNA controls, qPCR reaction buffer, primer\/probe mix, and IPC mix to support standard curve generation and assay QC.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePlatform compatibility:\u003c\/strong\u003e Validated on common qPCR instruments (SHENTEK-96S, ABI 7500, Bio-Rad CFX96) and adaptable to other real-time PCR systems.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-defined analysis settings:\u003c\/strong\u003e Recommended thermal cycling and analysis parameters (e.g., threshold = 0.02, manual Ct) provided to standardize quantitation.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual SV40LTA and E1A host cell DNA in cell substrates (e.g., HEK293T)\u003c\/li\u003e\r\n \u003cli\u003eResidual host cell DNA monitoring for upstream\/downstream bioprocessing and product release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and standard curve generation for absolute quantitation of SV40-derived sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A linear DNA Control (NNA019) — lyophilized powder ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A non-linear DNA Control (NNA020) — 50 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSV40LTA \u0026amp; E1A Primer \u0026amp; Probe MIX (NNC030) — 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) — 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store remaining DDB at 2–8 °C after opening per protocol)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read the Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory coat, and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Avoid contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect surfaces with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Reconstitute lyophilized control carefully: add 55 μL ddH2O, mix and let stand 10 minutes; perform serial dilutions as instructed.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer \u0026amp; Probe MIX, IPC MIX) from light and store at –20 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare at least five concentration points for the standard curve; perform method validation to select appropriate sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• DDB: store remaining unused DDB at 2–8 °C; if cloudy, heat to 37 °C until clear.\u003c\/p\u003e\r\n \u003cp\u003e• IPC criteria: mean Ct(IPC) should be within ±1.0 of NCS Ct(IPC); significant shifts indicate inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: SV40LTA ≤ 14.32 copies\/μL and E1A ≤ 17.79 copies\/μL (or use user-defined validation criteria).\u003c\/p\u003e\r\n \u003cp\u003e• Configure analysis parameters per instrument and software version; recommended settings include manual Ct and threshold = 0.02.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1403443.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) for this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit reports sensitivity to the level of 10^1 copies\/μL. Actual LOD\/LOQ should be confirmed during your laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR instruments including SHENTEK-96S, ABI 7500 (SDS v1.4 example) and Bio-Rad CFX96. Other instruments may be used with appropriate program and analysis adjustments.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I set up the qPCR thermal profile and analysis?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended program: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 (read fluorescence during annealing\/extension). Analysis: Manual Ct with threshold = 0.02 and Automatic Baseline; use Absolute Quantitation (standard curve).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I include and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude NTC, NCS, and at least five standard curve points (ST1–ST5; ST6 optional for linear control). IPC mean Ct should be within ±1.0 of NCS. NTC detection values should be ≤ 14.32 copies\/μL for SV40LTA and ≤ 17.79 copies\/μL for E1A, or use your validated criteria.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45191440335029,"sku":"1403443","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_SV40_LTA_E1A_DNA_Quantitation_Kit_2G.webp?v=1742280022"},{"product_id":"rnaprep-pure-plant-plus-kit","title":"RNAprep Pure Plant Plus Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe RNAprep Pure Plant Plus Kit (Polysaccharides \u0026amp; Polyphenolics-rich) is a silicon matrix purification RNA extraction kit developed for multiple plant samples with polysaccharides and polyphenolics. It is supplied with Buffer SL with superb lysis ability. High-purity total RNA without gDNA can be extracted from the fleshes of bananas, watermelons, apples, pears, the tubers of sweet potatoes, potatoes, as well as the leaves of cotton, rose, alfalfa, rice and white pine needles within 1 hour.\u003c\/p\u003e\n\u003ch3\u003eRequired Reagents\u003c\/h3\u003e\n\u003cp\u003eβ-mercaptoethanol, ethanol\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTargeted: It is specially formulated for plant samples that are difficult to extract, such as polysaccharides and polyphenolics-rich plants. The process is more optimized, and the results are reliable.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEfficient removal of gDNA: High-efficient DNase I is supplied for rapid removal of gDNA on the column.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEasy and fast: RNA extraction experiments can be completed within 1 hour.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSafe and low toxicity: No toxic organic reagents such as phenol and chloroform are needed.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eThis kit can be directly used for various molecular biology experiments such as RT-PCR, real time PCR, Northern Blot, Dot Blot, PolyA screening, in vitro translation, RNase protection analysis, and cloning, etc.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eDNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃\u003c\/p\u003e\n\u003cp\u003eOther reagents could be stored at room temperature (15-25℃)\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/GDP441-RNAprep_Pure_Plant_Plus_Kit-240514.pdf?v=1741324028\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eGDP441-RNAprep Pure Plant Plus Kit-240514\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Tiangen","offers":[{"title":"50 preps","offer_id":45157581324469,"sku":"GDP441","price":336.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RNAprepPurePlantPlusKit.jpg?v=1757413022"},{"product_id":"rnasimple-total-rna-kit","title":"RNAsimple Total RNA Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eThe RNAsimple Total RNA Kit adopts a new RNA extraction method based on guanidinium isothiocyanate\/phenol method. The Buffer RZ specifically designed by TIANGEN can remove genomic DNA and proteins from cells rapidly and efficiently, making the obtained RNA highly pure and stable.\u003c\/p\u003e\n\u003cp\u003eThis kit is used to separate total RNA from blood, animal cells, tissues, and plant tissues. Each spin column can process 50-100 mg tissue or 5x106 cells at a time and can process a large number of different samples simultaneously. The reaction can be completed in less than one hour, and the total RNA extracted has a higher yield, better purity, with no DNA and protein contamination, and can be used in various downstream experiments.\u003c\/p\u003e\n\u003ch3\u003eRequired Reagents\u003c\/h3\u003e\n\u003cp\u003eEthanol, chloroform\u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThe high-purity ready-to-use RNA is suitable for sensitive downstream applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eWide applications: The purified RNA can be applied to various experimental samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe experiment can be completed in 1 hour with simple operations.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eRT-PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNorthern Blot, Dot Blot.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eReal-Time PCR\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003ePolyA Screening, in vitro translation, RNase protection analysis, molecular cloning.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eStore at room temperature (15-25℃)\u003c\/p\u003e\n\u003cp\u003eBuffer RZ should be stored at 2-8℃ away from light\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/GDP419-RNAsimple_Total_RNA_Kit-240514.pdf?v=1741325187\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eGDP419-RNAsimple Total RNA Kit-240514\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Tiangen","offers":[{"title":"50 preps","offer_id":45157598658741,"sku":"GDP419","price":215.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_55e318e8-a3a3-4f38-93ef-365c15dd29f8.jpg?v=1741325369"},{"product_id":"animal-origin-residual-dna-sample-preparation-kit","title":"Animal-origin Residual DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered 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{\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e2-8 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit uses magnetic particle separation technology to extract residual DNA from a broad range of animal-derived biomaterials. The kit is designed for efficient and reproducible recovery of residual DNA from complex sample matrices including extracellular matrix, collagen and bio-absorbable membranes. Extracted DNA is compatible with downstream residual DNA quantitation by qPCR or PicoGreen assays. The kit supports manual sample preparation and automated extraction using the rHCDpurify system. Reagents are supplied for 50 extractions. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct number \/ Version\u003c\/td\u003e\r\n \u003ctd\u003eProduct No.: 1104193; User Guide Version: A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — residual DNA extraction from animal-derived biomaterials\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions \/ Extractions per kit\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eOverall kit shelf life\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eUp to 24 months (check label for expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic particles storage\u003c\/td\u003e\r\n \u003ctd\u003e2-8 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCold-stored reagents\u003c\/td\u003e\r\n \u003ctd\u003eGlycogen: -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC-treated H2O\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProteinase K digestion conditions\u003c\/td\u003e\r\n \u003ctd\u003eIncubate at 55 °C for 60 minutes (dry bath); adjust amount\/time for difficult samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding buffer (per sample)\u003c\/td\u003e\r\n \u003ctd\u003e200 µL Binding solution + 9 µL Glycogen + 0.2 µL Yeast tRNA (Do not add Yeast tRNA when using PicoGreen assay)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eBinding step volumes\u003c\/td\u003e\r\n \u003ctd\u003eAfter adding binding buffer, add 200 µL isopropanol and 30 µL magnetic particles per sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWash volumes\u003c\/td\u003e\r\n \u003ctd\u003eWash buffer A: 700 µL; Wash buffer B: 700 µL (per wash step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eElution\u003c\/td\u003e\r\n \u003ctd\u003eAdd 100–150 µL pre-warmed (70 °C) Elution buffer; incubate at 70 °C for 7 minutes, vortex intermittently\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMagnetic separation time\u003c\/td\u003e\r\n \u003ctd\u003eParticle separation typically ~3–5 minutes on a magnetic stand\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAutomation compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with rHCDpurify automated extraction (rHCD-193 program described)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls recommended\u003c\/td\u003e\r\n \u003ctd\u003eNegative control sample (NCS, 1×PBS) and sample extraction recovery control (ERC) recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eEnvironmental conditions for instrument\u003c\/td\u003e\r\n \u003ctd\u003eAmbient temperature should not be lower than 22 °C for automated runs; UV sterilization recommended before\/after runs\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation:\u003c\/strong\u003e Uses magnetic particle technology for efficient nucleic acid binding and purification from complex matrices.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eOptimized for animal-derived biomaterials:\u003c\/strong\u003e Validated for complex matrices such as extracellular matrix, collagen and bio-absorbable membranes.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility with downstream assays:\u003c\/strong\u003e Extracted DNA is suitable for qPCR quantitation and PicoGreen analysis.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFlexible workflow:\u003c\/strong\u003e Supports manual extraction and high-throughput automated extraction on the rHCDpurify system.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eIncluded controls and reagents:\u003c\/strong\u003e Includes reagents and recommended controls (NCS, ERC) to assess recovery and contamination.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eResidual DNA quantitation in animal-derived biomaterials\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of collagen, extracellular matrix products and bio-absorbable membranes\u003c\/li\u003e\r\n \u003cli\u003ePreclinical \/ manufacturing residual DNA assessment\u003c\/li\u003e\r\n \u003cli\u003eSample preparation for qPCR or fluorescence-based DNA assays (PicoGreen)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eWash buffer A (NND015) 15 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBinding solution (NND017) 10 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eElution buffer (NND020) 20 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003e1× PBS (NND042) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDEPC-treated H2O (NND045) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K Buffer (NND025) 5 mL × 1 bottle\u003c\/span\u003e\u003cstrong\u003eRoom temperature (if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMagnetic particles (NND030) 750 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eProteinase K (NND023) 500 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eGlycogen (NND035) 500 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eYeast tRNA (NND037) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to label\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle separation-based DNA extraction (manual or automated with rHCDpurify)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• Read MSDS and wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Add 20 mL anhydrous ethanol to Wash buffer A before first use; prepare Wash buffer B as 70% ethanol with ddH2O.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare Proteinase K digestion solution freshly; incubate samples at 55 °C for 60 min (adjust as needed).\u003c\/p\u003e\r\n \u003cp\u003e• Do not add Yeast tRNA when using PicoGreen DNA quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Resuspend magnetic particles thoroughly before use; avoid over-drying pellets—dry 30 s to 3 min depending on conditions.\u003c\/p\u003e\r\n \u003cp\u003e• Centrifuge after vortexing to collect liquid from tube cap and walls before magnetic separation.\u003c\/p\u003e\r\n \u003cp\u003e• Transfer eluate immediately after elution and perform downstream assays the same day to ensure accuracy.\u003c\/p\u003e\r\n \u003cp\u003e• Store magnetic particles at 2–8 °C; cold-stored reagents (e.g., glycogen) should be kept at -20 °C.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1104193.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: Which downstream assays are compatible with the extracted DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExtracted DNA is compatible with qPCR-based residual DNA quantitation and fluorescence-based assays such as PicoGreen. Do not add Yeast tRNA if using PicoGreen.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended digestion protocol for samples?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare Proteinase K digestion solution (20 µL Proteinase K + 100 µL Proteinase K Buffer + 80 µL DEPC H2O per sample) and incubate at 55 °C for 60 minutes. Adjust enzyme amount and digestion time for difficult-to-digest or cross-linked samples.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should Wash buffer A be prepared before first use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 20 mL of anhydrous ethanol to Wash buffer A (NND015) before first use as instructed in the manual.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Can I run the kit on an automated platform?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify system (program rHCD-193). Follow the plate layout and column reagent volumes specified in the manual and perform UV sterilization as recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171044843701,"sku":"1104193","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Animal-origin_Residual_DNA_Sample_Preparation_Kit.jpg?v=1742277138"},{"product_id":"residual-host-cell-dna-sample-preparation-kit-for-vaccines-shentek™","title":"Residual Host Cell DNA Sample Preparation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\n\u003cdiv class=\"shentek-wrapper\"\u003e\n\u003cdiv class=\"shentek-header-bar\"\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-item\"\u003e\n\u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\n\u003cdiv class=\"shentek-header-value\"\u003e2-8 °C \u0026amp; -20 °C (stable for 24 months)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003csection class=\"shentek-section shentek-section--divider\"\u003e\n\u003ch2 class=\"shentek-title\"\u003e\n Product Description\u003c\/h2\u003e\n\u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Host Cell DNA Sample Preparation Kit is a magnetic particle–based nucleic acid extraction kit specifically optimized for vaccine products. It is designed to stably and efficiently recover trace amounts of residual host-cell DNA from finished vaccines (e.g., Vero cell–derived rabies vaccines). The kit provides an integrated workflow when used with SHENTEK® host cell DNA qPCR detection kits and supports manual extraction or automated extraction using the rHCDpurify® system. Special sample processing methods can be provided for samples containing aluminum adjuvants or dextran.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-table-wrapper\"\u003e\n\u003ctable class=\"shentek-table\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth\u003eParameter\u003c\/th\u003e\n\u003cth\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct number\u003c\/td\u003e\n\u003ctd\u003eSK030206DM50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended reactions\u003c\/td\u003e\n\u003ctd\u003eReagents for 50 extractions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSample input volume\u003c\/td\u003e\n\u003ctd\u003e100 µL test sample (per extraction)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion reagents per sample\u003c\/td\u003e\n\u003ctd\u003e10 µL 5M NaCl, 30 µL Sample buffer (NND029), 15 µL Proteinase K (NND023)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDigestion conditions\u003c\/td\u003e\n\u003ctd\u003eIncubate at 55 °C for 60 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding reagents per sample\u003c\/td\u003e\n\u003ctd\u003e200 µL Binding solution (NND017) + 200 µL isopropanol + 10 µL Magnetic particles (NND030)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBinding procedure\u003c\/td\u003e\n\u003ctd\u003eVortex 5 minutes (medium speed); magnetic separation 3–5 minutes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash volumes\u003c\/td\u003e\n\u003ctd\u003e700 µL Wash buffer A (with ethanol added) then 700 µL Wash buffer B (70% ethanol)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution volume and conditions\u003c\/td\u003e\n\u003ctd\u003e50–100 µL Elution buffer (NND019); incubate at 70 °C for 7 minutes (vortex 2–3 times during incubation)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMagnetic particle handling\u003c\/td\u003e\n\u003ctd\u003eResuspend magnetic particles before use; allow particles to separate 3–5 minutes on magnet; air-dry 1–3 minutes before elution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCompatible automation\u003c\/td\u003e\n\u003ctd\u003erHCDpurify® automated extraction system (program rHCD-06DM50 \/ HCD-06DM50)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage stability\u003c\/td\u003e\n\u003ctd\u003eKit components stored under indicated conditions are stable up to 24 months (check label expiration)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCritical sample pH range\u003c\/td\u003e\n\u003ctd\u003epH 6.0–8.0 required; adjust with 1M HCl or 1M NaOH if outside range\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRecommended controls\u003c\/td\u003e\n\u003ctd\u003eNegative Control Sample (NCS) and Extraction Recovery Control (ERC) (ERC spike recommended 2–10× amount found in unspiked sample)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIntended use \/ Compliance\u003c\/td\u003e\n\u003ctd class=\"shentek-text-danger\"\u003eResearch Use Only (RUO) — sample preparation for downstream host cell DNA qPCR assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eMagnetic particle separation technology:\u003c\/strong\u003e Efficient capture and concentration of trace DNA using magnetic particles for high recovery.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVaccine-optimized workflow:\u003c\/strong\u003e Formulation and protocol optimized for finished vaccine matrices (e.g., Vero cell–derived rabies vaccine). Specific processing available for aluminum adjuvants or dextran-containing samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIntegrated with qPCR detection:\u003c\/strong\u003e Designed to work with SHENTEK® host cell DNA qPCR detection kits to provide a complete sample-to-result workflow.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eManual and automated compatibility:\u003c\/strong\u003e Protocols for manual extraction and for automated processing on the rHCDpurify® system (96 deep-well format) to support throughput needs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLow-volume, trace DNA recovery:\u003c\/strong\u003e Optimized to obtain trace amounts of residual host-cell DNA stably and reproducibly from complex vaccine matrices.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\n\u003cul class=\"shentek-list\"\u003e\n\u003cli\u003eSample pretreatment for residual host cell DNA testing in finished vaccines (e.g., Vero cell–derived rabies vaccine)\u003c\/li\u003e\n\u003cli\u003eQuality control testing during vaccine manufacture\u003c\/li\u003e\n\u003cli\u003eSample preparation prior to SHENTEK® host cell DNA qPCR assays\u003c\/li\u003e\n\u003cli\u003eAutomated high-throughput residual DNA extraction using rHCDpurify® system\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box\"\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eWash buffer A (NND015)\u003c\/span\u003e\u003cstrong\u003eRoom temperature (add 20 mL anhydrous ethanol before first use)\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eBinding solution (NND017)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eElution buffer (NND019)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eDilution buffer (NND022)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eSample buffer (NND029)\u003c\/span\u003e\u003cstrong\u003eRoom temperature\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eMagnetic particles (NND030)\u003c\/span\u003e\u003cstrong\u003e2-8 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-row\"\u003e\n\u003cspan\u003eProteinase K (NND023)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-box-note\"\u003e* Total: 50 extractions | Method: Magnetic particle–based nucleic acid extraction for downstream qPCR detection (manual or rHCDpurify automated)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\n\u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\n\u003cp\u003e• Follow Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, eyewear, lab coat).\u003c\/p\u003e\n\u003cp\u003e• Use separate laboratory areas for negative reagent preparation, positive\/sample processing, and PCR amplification to prevent cross-contamination.\u003c\/p\u003e\n\u003cp\u003e• Ensure ambient temperature is not lower than 22 °C before starting experiments.\u003c\/p\u003e\n\u003cp\u003e• Centrifuge tubes before opening to avoid contamination; handle carefully to avoid splashing.\u003c\/p\u003e\n\u003cp\u003e• Change gloves regularly and use dedicated lab coats\/masks\/hair covers in different areas.\u003c\/p\u003e\n\u003cp\u003e• Dispose of used tips and liquid waste per regulations; do not open PCR amplification products in non-designated areas.\u003c\/p\u003e\n\u003cp\u003e• During magnetic separation, rotate tubes slowly to accelerate particle aggregation; avoid disturbing particles when removing supernatant.\u003c\/p\u003e\n\u003cp\u003e• Avoid over-drying magnetic particles after wash (over-drying can cause incomplete elution).\u003c\/p\u003e\n\u003cp\u003e• Ensure no magnetic particles or liquid remain on tube caps or walls; if present, briefly centrifuge.\u003c\/p\u003e\n\u003cp\u003e• Perform DNA detection on the same day as extraction to ensure accuracy.\u003c\/p\u003e\n\u003cp\u003e• Before automated runs, confirm 96-well plate and sleeve are secured; UV-sterilize the instrument interior and wipe with 75% ethanol before and after runs.\u003c\/p\u003e\n\u003cp\u003e• Minimum interval between two extractions on rHCDpurify® should be 30 minutes; UV sterilize instrument for at least 15 minutes between runs.\u003c\/p\u003e\n\u003cp\u003e• Collect more than 40 µL of eluate for downstream assay; transfer eluate immediately after automated run to new microcentrifuge tubes.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--lg\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-card\"\u003e\n\u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\n\u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\n\u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\u003c\/h3\u003e\n\u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\n\u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030206DM50.pdf\" target=\"_blank\"\u003e \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection class=\"shentek-section\"\u003e\n\u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\n\u003cdiv class=\"shentek-faq\"\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample volume is required per extraction?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eUse 100 µL test sample per extraction. The protocol specifies addition of 10 µL 5M NaCl and 30 µL Sample buffer prior to Proteinase K digestion.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should the magnetic particles and Proteinase K be stored?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eStore magnetic particles at 2–8 °C. Store Proteinase K at -20 °C. Other buffers are stored at room temperature; kit components are stable under indicated conditions for up to 24 months (check label expiration).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ3: Can the kit be used with automated extraction instruments?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003eYes. The kit is compatible with the rHCDpurify® automated system (program: rHCD-06DM50 \/ HCD-06DM50) using a 96 deep-well plate layout; the manual also provides program settings and plate layout guidance.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-item\"\u003e\n\u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if I observe low recovery of nucleic acids?\u003c\/div\u003e\n\u003cdiv class=\"shentek-faq-answer\"\u003ePossible causes include missing ethanol in Wash buffer A, magnetic particles sticking to tube walls, low ionic strength, out-of-range sample pH, or incorrect storage of magnetic particles. Solutions: add ethanol to Wash buffer A per instructions, vortex\/heat to release particles from tube walls (70 °C for 2 min) and re-vortex, adjust ionic strength with 5M NaCl, adjust sample pH to neutral, and store magnetic particles at 2–8 °C (do not store at -20 °C).\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003cdiv class=\"shentek-notice\"\u003e\n\u003cdiv class=\"shentek-notice-header\"\u003e\n\u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\n \u003c\/svg\u003e\n\u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-body\"\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"shentek-notice-item\"\u003e\n\u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\n\u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or official compliance testing.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"50 Extractions","offer_id":45171074105525,"sku":"SK030206DM50","price":1010.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Host_Cell_DNA_Sample_Preparation_Kit_For_Vaccines.jpg?v=1742277270"},{"product_id":"residual-pichia-pastoris-dna-quantitation-kit-shentek™","title":"Residual Pichia pastoris DNA Quantitation Kit SHENTEK™","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Pichia Pastoris DNA Quantitation Kit is a fluorescence-based quantitative PCR (FAM) assay designed to quantify residual Pichia pastoris host-cell DNA in biopharmaceutical materials across process stages (from in-process samples to final products). The kit includes a Pichia Pastoris DNA Control as a reference standard and provides rapid, specific quantitation with reliable sensitivity at the fg level. The kit workflow supports DNA sample dilution\/standard curve preparation, qPCR reaction mix setup and absolute quantitation using a standard curve. Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, StepOne Plus). For DNA extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Pichia pastoris DNA in biopharmaceutical samples (in-process and final products).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReported quantitative range (from supplied standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 – 300 pg\/µL (equivalent to ~30 fg – 300 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReliable quantitation reported at the fg level; lowest supplied standard 0.03 pg\/µL (30 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (qPCR mix 20 µL + sample 10 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003ePichia Pastoris qPCR Reaction Buffer 17 µL + Pichia Pastoris Primer\u0026amp;Probe MIX 3 µL -\u0026gt; 20 µL qPCR mix\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eProbe-based qPCR with FAM reporter dye (no quencher specified in kit instructions); use ROX as passive reference dye where applicable\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eABI 7500, CFX96, StepOne Plus, Linegene 9600plus, SHENTEK-96S and similar real-time PCR systems\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt \/ analysis settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02; Automatic baseline recommended. NTC Ct should be ≥ 35 cycles (or per internal validation). NCS Ct should be larger than mean Ct of lowest standard.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSpike recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eSpiked ERC recoveries required to be within 50% - 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ storage\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; kit stability up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003eExample workflow uses 100 µL test sample for preparation and 10 µL purified sample per qPCR reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Provides reliable quantitation at the fg level (lowest supplied standard 0.03 pg\/µL = 30 fg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eProbe-based specific detection:\u003c\/strong\u003e FAM-labeled probe assay offers high specificity for Pichia pastoris DNA fragments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard control:\u003c\/strong\u003e Includes Pichia Pastoris DNA Control for absolute quantitation and serial dilution standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003ePre-formulated mix:\u003c\/strong\u003e Supplied reaction buffer and primer\/probe mix simplify qPCR setup and reduce preparation time.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWide instrument compatibility:\u003c\/strong\u003e Validated with common real-time PCR platforms (ABI 7500, CFX96, StepOne Plus, Linegene, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow-ready for QC:\u003c\/strong\u003e Designed for in-process and final product host-cell DNA monitoring with recommended plate layouts and analysis settings.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Pichia pastoris host-cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process testing and final product quality control for recombinant proteins produced in Pichia pastoris\u003c\/li\u003e\r\n \u003cli\u003eRoutine host-cell DNA monitoring during downstream purification and formulation\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike-recovery experiments for DNA residual assays\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris DNA Control (Part No. NNA007) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris qPCR Reaction Buffer (Part No. NNB005) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePichia Pastoris Primer \u0026amp; Probe MIX (Part No. NNC041) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: thaw\/mix at 2–8 °C for use; unused DDB may be stored at 2–8 °C per instructions)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, clothing and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: UV-irradiate tabletop, pipettes and tubes for 30 minutes and disinfect with 75% alcohol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents from light; handle primer\/probe and buffers on ice where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice and vortex to mix before use; spin briefly in microcentrifuge.\u003c\/p\u003e\r\n \u003cp\u003e• Check kit expiration date and storage conditions on labels prior to use.\u003c\/p\u003e\r\n \u003cp\u003e• Maintain recommended plate layout, replicate strategy and include NTC, NCS and ERC controls.\u003c\/p\u003e\r\n \u003cp\u003e• Spike recovery acceptance criteria: 50%–150%.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 cycles (or defined by internal validation).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030205P100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit supports reliable quantitation at the fg level. The supplied standard curve includes a lowest standard of 0.03 pg\/µL (30 fg\/µL). Actual LOD in a given matrix should be confirmed by in-house validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the Pichia Pastoris DNA Control and DNA Dilution Buffer (DDB) on ice. Prepare ST0 at 3000 pg\/µL then perform serial 1:10 dilutions to produce ST1–ST5: 300, 30, 3, 0.3 and 0.03 pg\/µL. Include at least five concentrations for the standard curve and run in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the recommended qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse 95 °C for 10:00 (activation, 1 cycle), then 40 cycles of 95 °C for 00:15 (denaturation) and 60 °C for 01:00 (annealing\/extension, fluorescence read). Adjust settings if required by your instrument\/software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should reagents be stored after thawing?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C per label. DNA Dilution Buffer (DDB) may be kept at 2–8 °C for short-term use; follow the instructions regarding storage of unused DDB and inspect for cloudiness\/precipitate before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171125813429,"sku":"SK030205P100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Pichia_Pastoris_DNA_Quantitation_Kit.jpg?v=1742277610"},{"product_id":"residual-hansenula-polymorpha-dna-quantitation-kit-shentek®","title":"Residual Hansenula polymorpha DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hansenula polymorpha DNA Quantitation Kit is designed to quantitatively detect and measure residual Hansenula polymorpha host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses fluorescent quantitative PCR (real-time qPCR) with a specific primer\/probe mix to provide rapid, specific and reliable quantitation at the femtogram (fg) level. The kit is supplied with DNA control material and reagents for sample dilution and qPCR reaction setup. For DNA extraction\/sample preparation procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Labeling\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFor Research Use Only (RUO)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format \/ Yield\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST1 300 pg\/µL; ST2 30 pg\/µL; ST3 3 pg\/µL; ST4 0.3 pg\/µL; ST5 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical range (implied)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL to 300 pg\/µL (30 fg\/µL to 300 pg\/µL) — demonstrates femtogram-level detection sensitivity\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denature 95 °C 0:15 (40 cycles); Anneal\/Extend 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd\u003eqPCR MIX per reaction: 20 µL (17 µL qPCR Reaction Buffer + 3 µL Primer\u0026amp;Probe MIX); Final reaction: 30 µL (add 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR analysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02; Automatic baseline; NTC Ct ≥ 35 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ recovery criteria\u003c\/td\u003e\r\n \u003ctd\u003eERC recovery acceptable range: 50%–150%; NCS Ct should be larger than mean Ct of lowest standard curve concentration (or less than LOQ if LOQ \u0026lt; lowest standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage \u0026amp; stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C; kit components stable for up to 24 months (check expiration on labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Mx3000P, StepOne Plus, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual Hansenula polymorpha DNA at femtogram-level sensitivity (standard curve includes down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFluorescent real-time qPCR:\u003c\/strong\u003e Uses a validated primer \u0026amp; probe mix with FAM reporter for specific and rapid quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete kit for quantitation:\u003c\/strong\u003e Includes DNA control, qPCR reaction buffer, primer \u0026amp; probe mix and DNA dilution buffer — sufficient reagents for 100 reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Protocols and settings provided for commonly used real-time PCR platforms; assay template provided for absolute quantitation (standard curve).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQC and controls included:\u003c\/strong\u003e Guidance and materials for NTC, NCS and ERC controls; recommended acceptance criteria (NTC Ct ≥35; ERC recovery 50–150%).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Hansenula polymorpha DNA in final drug products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and routine QC testing for host-cell DNA\u003c\/li\u003e\r\n \u003cli\u003eResearch applications involving detection of Hansenula polymorpha DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha DNA Control (NNA008)\u003c\/span\u003e\u003cstrong\u003e-20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha qPCR Reaction Buffer (NNB007)\u003c\/span\u003e\u003cstrong\u003e-20 °C (850 μL × 2 tubes) — protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHansenula polymorpha Primer \u0026amp; Probe MIX (NNC040)\u003c\/span\u003e\u003cstrong\u003e-20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate working surfaces, pipettes and tubes (UV irradiation 30 minutes recommended) and disinfect with 75% ethanol.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge to mix.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C; check expiration dates.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer is cloudy, heat to 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls (NTC, NCS, ERC) and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• Recommended qPCR analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: NTC Ct ≥ 35 cycles or undetermined; ERC recovery 50%–150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030222HA100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level detection; the provided standard curve covers 0.03 pg\/µL (30 fg\/µL) to 300 pg\/µL. Users should validate LOD\/LOQ in their own laboratory and instrument setup.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR program and settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRecommended cycling: 95 °C 10:00 (activation); 40 cycles of 95 °C 0:15 (denature) and 60 °C 1:00 (anneal\/extend, read fluorescence). Analysis settings: Manual Ct, Threshold = 0.02, Automatic baseline. Adjust for your instrument if required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions of the provided DNA Control in DNA Dilution Buffer to generate at least five concentrations (example: 300, 30, 3, 0.3, 0.03 pg\/µL). Run each standard in triplicate for absolute quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are acceptable QC criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or Ct ≥ 35 cycles. NCS Ct should be larger than the mean Ct of the lowest standard or below the LOQ if LOQ is lower than the lowest standard. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171150553269,"sku":"SK030222HA100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hansenula_Polymorpha_DNA_Quantitation_Kit.jpg?v=1742277725"},{"product_id":"residual-saccharomyces-cerevisiae-dna-quantitation-kit-shentek®","title":"Residual Saccharomyces¬†cerevisiae DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Saccharomyces cerevisiae DNA Quantitation Kit is designed to quantify residual Saccharomyces cerevisiae DNA in biopharmaceutical materials across process stages from in-process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with analytical sensitivity at the femtogram (fg) level. For DNA extraction guidance, refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). The kit supplies reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eWorking standard range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSerial standards prepared from DNA control: 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest standard (example)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.03 pg\/µL (30 fg\/µL) included as lowest standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (document states fg-level sensitivity); practical detection guided by lowest standard and lab validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eNot explicitly specified; users should determine LOQ by laboratory validation. The lowest provided standard is 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eqPCR master mix per reaction 20 µL + 10 µL sample = 30 µL total reaction volume\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eMaster mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eSaccharomyces cerevisiae qPCR Reaction Buffer 17 µL + Primer \u0026amp; Probe MIX 3 µL (total 20 µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C 10:00 (1 cycle); Denature: 95 °C 00:15 (40 cycles); Anneal\/Extend: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection dye \/ probe\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; no quencher specified in instructions (primer \u0026amp; probe mix provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCt threshold \/ analysis settings\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecommended Manual Ct threshold = 0.02; baseline = Automatic. NTC Ct should be ≥ 35.00 cycles or undetermined (or laboratory-specific criteria).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIncludes DNA control for standard curve, Negative Control Sample (NCS), No Template Control (NTC) and Extraction Reference Control (ERC). ERC recovery acceptance: 50%–150%. Ct(NCS) should be larger than mean Ct of lowest standard (or below LOQ if LOQ proven lower).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReplicates\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, samples and controls\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided kit size\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stable for up to 24 months when stored appropriately (see component-level storage)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended consumables\u003c\/td\u003e\r\n \u003ctd\u003eDNase-free low-retention tubes and tips; 96-well qPCR plates with sealing film or 8-strip PCR tubes with caps\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Designed to detect Saccharomyces cerevisiae DNA down to the femtogram (fg) level; includes low-concentration standards (down to 0.03 pg\/µL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and reliable:\u003c\/strong\u003e Contains optimized primer \u0026amp; probe mix and qPCR reaction buffer for specific amplification and reproducible quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Supplies DNA control, qPCR reaction buffer and primer \u0026amp; probe mix to simplify assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated for use on multiple real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, CFX96, LineGene 9600 plus, Mx3000PTM, StepOne Plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eLong shelf life:\u003c\/strong\u003e Kit components are stable for up to 24 months when stored under recommended conditions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Saccharomyces cerevisiae DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eHost cell DNA residue testing during upstream\/downstream process development and release testing (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and spike\/recovery experiments using ERC samples\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae DNA Control (Part No. NNA009) — 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae qPCR Reaction Buffer (Part No. NNB006) — 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSaccharomyces cerevisiae Primer \u0026amp; Probe MIX (Part No. NNC039) — 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (note: remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and change tips frequently.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex to mix and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial dilution as instructed; include at least five concentrations for the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Triplicate measurements are recommended for standards, samples and controls.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35 or be undetermined; if not, investigate contamination.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery should be between 50% and 150%; verify extraction efficiency and assay performance.\u003c\/p\u003e\r\n \u003cp\u003e• Determine LOQ and assay validation parameters within your laboratory according to regulatory guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101103.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 plus, Agilent Mx3000PTM and StepOne Plus. If using other instruments, follow instrument-specific setup and validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the lowest standard concentration provided and does it represent the kit LOQ?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest provided standard is 0.03 pg\/µL (30 fg\/µL). The kit states fg-level sensitivity, but you should determine the limit of quantification (LOQ) for your matrix by laboratory validation; the provided lowest standard can be used as a reference point.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I handle and store unused DNA Dilution Buffer (DDB)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore remaining unused DDB at 2–8 °C. If the solution becomes cloudy or precipitated, warm to 37 °C until clear before use.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be ≥ 35 cycles or undetermined. Ct of NCS should be larger than the mean Ct of the lowest standard (or below LOQ if the LOQ is proven lower than the lowest standard). ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171176636597,"sku":"1101103","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Saccharomyces_Cerevisiae_DNA_Quantitation_Kit.jpg?v=1742277789"},{"product_id":"residual-cho-dna-quantitation-kit-shentek®","title":"Residual CHO DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Quantitation Kit is designed for quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA in a variety of biopharmaceutical products. The kit uses quantitative PCR (absolute quantitation with a standard curve) to perform rapid and specific quantitation of residual DNA at the femtogram level. For nucleic acid extraction and sample preparation guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. SK030203D100). The kit is supplied as reagents for 100 reactions and is intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical products (research use only).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection principle\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by quantitative PCR (standard curve).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuantitation range (standards)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard concentrations prepared from CHO DNA Control: ST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL; ST6 = 0.003 pg\/μL. (Implied dynamic range: 300 to 0.003 pg\/μL using provided standards.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantitation \/ sensitivity\u003c\/td\u003e\r\n \u003ctd\u003eStandards include down to 0.003 pg\/μL (ST6). No explicit LOQ\/LOD numeric claim provided; users should validate limit of quantitation (QL) in their own laboratory.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or standard).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified sample (or 10 μL standard\/controls as described).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix provided\u003c\/td\u003e\r\n \u003ctd\u003eReady-to-use CHO qPCR MIX supplied (mix thoroughly before use); protect from light.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C for 10:00; 40 cycles of Denaturation 95 °C for 0:15 and Annealing\/Extension 60 °C for 1:00 (fluorescence read at 60 °C).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCycling count\u003c\/td\u003e\r\n \u003ctd\u003e40 cycles\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — ERC recovery\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eRecovery of Extraction Reference Control (ERC) samples should be between 50% and 150%.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria — NTC \/ NCS\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC: Ct should be Undetermined or not less than 35.00 (or laboratory-specific criteria). NCS Ct should be larger than the mean Ct of the lowest standard; if proven QL is lower than minimum standard concentration, NCS detected value should be less than QL.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR systems including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600 Plus, Mx3000P, qTOWER 3 G, StepOnePlus (consult instrument documentation for setup).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components may be stored under recommended conditions for up to 24 months (check label expiration date).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended analysis settings (example ABI 7500 SDS v1.4)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 and Automatic Baseline; set task types for standard, unknown, NTC as described.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of detecting residual CHO DNA down to femtogram-level concentrations (standards provided to 0.003 pg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR mix:\u003c\/strong\u003e Provided CHO qPCR MIX formulated for absolute quantitation—minimizes preparation steps and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete standard curve control:\u003c\/strong\u003e Includes CHO DNA Control and DNA Dilution Buffer to prepare a 6-point standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR platforms; instrument-specific setup instructions included.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow guidance:\u003c\/strong\u003e User guide includes serial dilution protocol, plate layout examples, thermal cycling program, and acceptance criteria (ERC recovery, NTC\/NCS rules).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics production (host cell DNA monitoring)\u003c\/li\u003e\r\n \u003cli\u003eProcess development and purification monitoring for cell-derived products\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and release testing in research settings\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (Part No. NNA001)\u003c\/span\u003e\u003cstrong\u003e50 μL × 1 tube; store at –20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO qPCR MIX (Part No. NNC001)\u003c\/span\u003e\u003cstrong\u003e1 mL × 2 tubes; store at –20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e1.5 mL × 3 tubes; store at –20 °C (remaining unused DDB may be stored at 2–8 °C; if cloudy, warm to 37 °C to clarify)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR (absolute quantitation, standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only. Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eye protection, mask, gloves, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and centrifuge briefly; mix CHO qPCR MIX thoroughly and protect from light.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument-specific setup and analysis parameters; example settings are provided for ABI 7500 SDS v1.4 but may need adjustment for other platforms.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at recommended temperatures; per USP \u0026lt;659\u0026gt; items with –20 °C recommendation should be stored within ±10 °C (–30 °C to –10 °C).\u003c\/p\u003e\r\n \u003cp\u003e• Validate limit of quantitation (QL) and other performance metrics in your laboratory prior to routine testing.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure NTC (no template control) is undetermined or Ct ≥ 35 and NCS meets acceptance criteria before reporting sample results.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030201C100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the sensitivity of the kit and how low can it quantify CHO DNA?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve includes concentrations down to 0.003 pg\/μL (ST6). The kit enables femtogram-level detection, but users should perform laboratory validation to determine the assay’s limit of quantitation (QL) under their specific conditions.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and sample input volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 μL (20 μL CHO qPCR MIX + 10 μL sample or standard). Use 10 μL of purified test sample per reaction as described in the user guide.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should reagents be stored and what is kit stability?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore CHO DNA Control, CHO qPCR MIX and DNA Dilution Buffer at –20 °C (protect qPCR mix from light). Kit components are stable for up to 24 months; check expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC shows amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC (no template control) is amplified (Ct \u0026lt; 35 or determined), suspect contamination. Decontaminate workspace and consumables (UV, 75% ethanol), re-prepare master mixes and controls, and repeat the assay. Verify pipetting technique and use fresh aliquots of reagents.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171252527285,"sku":"SK030201C100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Quantitation_Kit.jpg?v=1742278019"},{"product_id":"residual-ns0-sp2-0-dna-quantitation-kit-shentek®","title":"Residual NS0 \u0026 SP2\/0 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual NS0\u0026amp;SP2\/0 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical matrices (in-process samples to final products). The kit provides NS0\u0026amp;SP2\/0 DNA Control as a reference standard and contains qPCR Reaction Buffer, NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX, and DNA Dilution Buffer. The assay is designed for sensitive, specific and rapid quantitation at the femtogram (fg) level. Reagents in this kit are sufficient for 100 reactions. For DNA extraction procedures refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191). For Research Use Only (RUO).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual NS0 and SP2\/0 host cell DNA in biopharmaceutical products (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR using a supplied standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDesigned to quantitate at femtogram (fg) level; lowest kit standard = 0.003 pg\/µL (3 fg\/µL). Laboratory validation required to establish LOD\/LOQ.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL (ST0 stock: 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + NS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX 3 µL = 20 µL per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (data acquisition during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real-time PCR instruments (examples: SHENTEK-96S, ABI 7500, Bio-Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥ 35 cycles or undetermined (or per lab validation); Ct of NCS should be \u0026gt; mean Ct of lowest standard or below validated LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable for up to 24 months when stored as instructed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReference \/ related product\u003c\/td\u003e\r\n \u003ctd\u003eFor extraction information see SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitation at femtogram (fg) level; lowest supplied standard 0.003 pg\/µL\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Supplies DNA control and primer\/probe mix for construction of standard curve and absolute quantitation\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX minimize assay setup variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated for use on multiple common real-time PCR platforms\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eQuality controls included:\u003c\/strong\u003e Includes NS0\u0026amp;SP2\/0 DNA Control for standard curve and guidance for NTC, NCS and ERC controls\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual NS0 host cell DNA in biopharmaceutical process intermediates and final drug substance\/formulation\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual SP2\/0 host cell DNA in biopharmaceutical samples\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA testing in QC and R\u0026amp;D (Research Use Only)\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 DNA Control (Part No. NNA021)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 µL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eNS0\u0026amp;SP2\/0 Primer \u0026amp; Probe MIX (Part No. NNC029)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 µL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes (remaining unused DDB store 2–8 °C; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, laboratory clothing and gloves\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables and dedicated workspace\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate workspace, pipettes and tubes with UV for 30 minutes and 75% ethanol before setup\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly prior to use\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates\/strips and spin briefly before running qPCR\u003c\/p\u003e\r\n \u003cp\u003e• Establish laboratory-specific LOQ\/LOD by validation; NTC Ct acceptance: ≥ 35 cycles or undetermined\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance: 50%–150%\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030208N100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration standard provided in the kit and what sensitivity can I expect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe lowest supplied standard (ST6) is 0.003 pg\/µL (3 fg\/µL). The kit is designed to enable quantitation at the femtogram level; however, actual limit of detection (LOD) and limit of quantitation (LOQ) should be established by laboratory validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the NS0\u0026amp;SP2\/0 DNA Control and DNA Dilution Buffer at 2–8 °C. Dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to prepare ST1–ST6 (ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL). Include at least five concentrations on the standard curve for quantitation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What qPCR cycling conditions and reaction setup should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 µL reaction volume (20 µL qPCR mix + 10 µL sample). Cycling: Activation 95 °C for 10:00 (1 cycle); Denaturation 95 °C for 0:15 (40 cycles); Annealing\/Extension 60 °C for 1:00 with fluorescence read during this step. Prepare qPCR mix per reaction: 17 µL qPCR Reaction Buffer + 3 µL Primer \u0026amp; Probe MIX.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How do I interpret controls and QC criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35 (or per lab validation). NCS Ct should be larger than mean Ct of lowest standard in the curve or below validated LOQ. ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171267436725,"sku":"SK030208N100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_NS0_SP2_0_DNA_Quantitation_Kit.jpg?v=1742278378"},{"product_id":"residual-vero-dna-154-quantitation-kit-shentek®","title":"Residual Vero DNA-154 Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Vero DNA-154 Quantitation Kit is a research-use qPCR kit for quantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process to final products). The kit uses a fluorescent quantitative PCR (FAM reporter) assay with a pre-formulated primer \u0026amp; probe mix and Vero DNA Control standard to enable rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit supplies Vero DNA Control as a reference standard and DNA Dilution Buffer for standard curve preparation. For nucleic acid extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (TaqMan-style probe); absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (documented serial dilutions down to 0.0003 pg\/μL = 0.3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003ePrepared from Vero DNA Control: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction (20 μL qPCR MIX + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + Vero Primer \u0026amp; Probe MIX-154 3 μL = 20 μL qPCR MIX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReporter \/ Reference dyes\u003c\/td\u003e\r\n \u003ctd\u003eReporter: FAM; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR instrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus (and similar real-time PCR systems)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTriplicates recommended\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended for standards, controls and test samples\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality criteria \/ acceptance\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate should be 50%–150%; Ct of NTC should be ≥35 or undetermined; Ct of NCS should be larger than mean Ct of lowest standard (or below validated LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months when stored as instructed (-20 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating Vero host DNA down to the femtogram level using an optimized primer\/probe assay and standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes pre-formulated Vero Primer \u0026amp; Probe MIX-154 for consistent, specific detection.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSupplied reference standard:\u003c\/strong\u003e Vero DNA Control provided for preparation of an 8-point serial dilution standard curve for absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with multiple qPCR platforms:\u003c\/strong\u003e Validated workflows and example settings included for several common real-time PCR instruments.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eUser guidance for QC:\u003c\/strong\u003e Includes instructions for NTC, NCS, ERC controls and acceptance criteria (ERC recovery 50%–150%, NTC Ct ≥35).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Vero host cell DNA in biopharmaceutical process samples\u003c\/li\u003e\r\n \u003cli\u003eQuality control testing of drug substance and drug product for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of downstream purification steps\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA quantitation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero DNA Control (NNA010)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eVero Primer \u0026amp; Probe MIX-154 (NNC018)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 300 μL × 1 tube; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic procedures.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, clothing and gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points and run samples in triplicate when possible.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery acceptance range: 50%–150%; ensure NTC Ct ≥35 or undetermined.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030227V100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eUse a 30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample). qPCR MIX per reaction is composed of 17 μL qPCR Reaction Buffer + 3 μL Vero Primer \u0026amp; Probe MIX-154.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What standard curve concentrations should I prepare?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare serial dilutions from the provided Vero DNA Control as follows: ST0 3000 pg\/μL; ST1 300 pg\/μL; ST2 30 pg\/μL; ST3 3 pg\/μL; ST4 0.3 pg\/μL; ST5 0.03 pg\/μL; ST6 0.003 pg\/μL; ST7 0.0003 pg\/μL. Include at least five concentration points in the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be within 50%–150%. The Ct for NTC should be ≥35 or undetermined. The Ct for NCS should be greater than the mean Ct of the lowest standard (or below validated LOQ if LOQ is lower than the lowest standard).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR platforms including SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600plus, and similar instruments. Adjust analysis settings to your instrument\/software as needed.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171280609461,"sku":"SK030227V100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Vero_DNA-154_Quantitation_Kit.jpg?v=1742278786"},{"product_id":"residual-mdck-dna-quantitation-kit-shentek®","title":"Residual MDCK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 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600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MDCK DNA Quantitation Kit is designed to quantitate residual MDCK host cell DNA in biopharmaceutical workflows, from in‑process samples to final products. The kit uses quantitative PCR (qPCR) for rapid, specific and reliable absolute quantitation at the femtogram (fg) level. The kit includes a MDCK DNA control, primer \u0026amp; probe mix, reaction buffer and DNA dilution buffer. For nucleic acid extraction guidance, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use \/ Application\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical samples (in‑process and final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitative PCR (qPCR), absolute quantitation using standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ LOD\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eClaims detection at femtogram (fg) level; lowest provided standard concentration = 0.003 pg\/μL (3 fg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Linear range (provided)\u003c\/td\u003e\r\n \u003ctd\u003eStandard concentrations prepared: 3000, 300, 30, 3, 0.3, 0.03, 0.003 pg\/μL (ST0–ST6); at least five concentrations recommended for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of Quantitation (LOQ)\u003c\/td\u003e\r\n \u003ctd\u003eLOQ not explicitly specified; protocol notes use of validated LOQ; if LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal qPCR reaction volume = 30 μL (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + MDCK Primer \u0026amp; Probe MIX 3 μL = 20 μL qPCR MIX per reaction\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15 (40 cycles); Annealing\/Extension: 60 °C 1:00 (read fluorescence during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eCompatible with common real‑time PCR instruments (SHENTEK‑96S, ABI 7500, Bio‑Rad CFX96, StepOne Plus, LineGene 9600, Mx3000P, etc.)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; Negative Control Sample (NCS) Ct should be larger than mean Ct of lowest standard (or less than LOQ if validated LOQ is lower); ERC recovery acceptance: 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTotal reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as labeled Product No.: SK030209M100)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components storable under appropriate conditions up to 24 months; individual components stored at -20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay readout \/ analysis settings (example)\u003c\/td\u003e\r\n \u003ctd\u003eRecommended analysis: Manual Ct, threshold for MDCK‑DNA = 0.02, automatic baseline; set tasks (Standard, Unknown, NTC) and verify slope, intercept, R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detects residual MDCK DNA down to the femtogram level (lowest provided standard 0.003 pg\/μL = 3 fg\/μL).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Standard‑curve based absolute quantitation using supplied MDCK DNA control and serial dilutions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use primer\/probe mix:\u003c\/strong\u003e Includes validated MDCK Primer \u0026amp; Probe MIX to simplify assay setup and improve reproducibility.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated for use on multiple common real‑time PCR platforms (ABI 7500, CFX96, StepOne Plus, LineGene, Mx3000P, SHENTEK‑96S, etc.).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eKit format:\u003c\/strong\u003e Provides reagents for 100 reactions with pre‑measured components (control, buffer, primer\/probe mix and dilution buffer).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MDCK host cell DNA in biopharmaceutical process development\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support for final biopharmaceutical products (research use)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for MDCK host cell DNA detection\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK DNA Control (NNA006) — 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) — 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMDCK Primer \u0026amp; Probe MIX (NNC024) — 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) — 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (absolute quantitation using standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (not for diagnostic or therapeutic use).\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low‑retention consumables; decontaminate workspace and pipettes with UV (30 min) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and spin briefly; store unused DNA Dilution Buffer at 2–8 °C.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light‑sensitive reagents (qPCR Reaction Buffer) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, ERC and standard curve in each run.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Follow instrument\/software‑specific parameter settings; example settings provided for ABI 7500 with SDS v1.4.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/SK030209M100.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest standard concentration provided and what is the claimed sensitivity?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided serial dilution scheme includes standards down to 0.003 pg\/μL (3 fg\/μL). The kit is intended to enable quantitation at the femtogram level.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and total reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare a 20 μL qPCR mix per reaction (17 μL qPCR Reaction Buffer + 3 μL Primer \u0026amp; Probe MIX) and add 10 μL sample\/standard for a total reaction volume of 30 μL.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the qPCR cycling conditions?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample program: Activation 95 °C 10:00 (1 cycle); Denaturation 95 °C 0:15 (40 cycles); Annealing\/extension 60 °C 1:00 with fluorescence read during this step.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What acceptance criteria should be used for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than the mean Ct of the lowest standard (or below validated LOQ if applicable); ERC recovery should be between 50% and 150%.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171293880501,"sku":"SK030209M100","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MDCK_DNA_Quantitation_Kit.jpg?v=1742279376"},{"product_id":"residual-sf9-acnpv-dna-quantitation-kit-shentek®","title":"Residual Sf9 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 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8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Sf9\u0026amp;AcNPV DNA Quantitation Kit is designed for quantitative measurement of residual Sf9 and AcNPV host cell DNA in biopharmaceutical materials at different processing stages (in-process to final products). The kit uses a multiplex quantitative PCR (qPCR) assay with internal positive control (IPC) to provide rapid, specific and reliable quantitation down to femtogram-level sensitivity. Supplied reagents include DNA control standards for Sf9 and AcNPV, primer\/probe mixes, IPC mix, qPCR reaction buffer and DNA dilution buffer. The kit is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A). Refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction guidance.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (Sf9)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e300 pg\/µL to 0.003 pg\/µL (standard curve points: 300, 30, 3, 0.3, 0.03, 0.003 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (AcNPV)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e5.14×10^6 to 5.14×10^1 copies\/µL (equivalent mass: 20 to 0.0002 pg\/µL; standard curve points provided for both copies\/µL and pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitivity down to femtogram (fg) level implied by standard curve lowest points (Sf9: 0.003 pg\/µL = 3 fg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction format \u0026amp; volumes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReaction total volume 30 µL per well (20 µL qPCR MIX + 10 µL sample).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL; Sf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 µL; IPC MIX 1.3 µL (total 20 µL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eActivation: 95 °C for 10:00 (1 cycle); Denaturation: 95 °C for 0:15; Annealing\/Extension: 60 °C for 1:00 (read fluorescence). 40 cycles.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument channels \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eSf9-DNA: FAM; AcNPV-DNA: CY5; IPC: VIC; Passive reference dye: ROX (ABI 7500 example).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ quantitation settings\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold 0.02 for Sf9-DNA, AcNPV-DNA and IPC; use Absolute Quantitation (Standard Curve) analysis.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC: Ct(sample) should be within ±1.0 Ct of Ct(NCS) — large deviation indicates inhibition. NTC: Ct ≥ 35.00 or undetermined. NCS Ct should be larger than mean Ct of lowest standard and show VIC amplification.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNumber of reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions (as shipped).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (see component-specific storage: most components -20 °C; protect qPCR Reaction Buffer from light).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThreshold \/ baseline guidance\u003c\/td\u003e\r\n \u003ctd\u003eThreshold set to 0.02; Automatic baseline recommended when using provided settings.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR:\u003c\/strong\u003e Simultaneous detection and quantitation of Sf9-DNA, AcNPV-DNA and IPC in one reaction using distinct fluorescent channels (FAM, CY5, VIC).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Assay standard curve includes femtogram-level concentrations (Sf9 down to 0.003 pg\/µL) enabling detection at fg scale.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC provided to monitor PCR inhibition and overall reaction performance for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated primer\/probe mix, IPC mix and reaction buffer simplify setup and reduce pipetting errors.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated examples and guidance provided for multiple real-time PCR platforms (e.g., SHENTEK-96S, ABI 7500, CFX96, FQD-96A).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eStandardized quantitation:\u003c\/strong\u003e Supplied Sf9 \u0026amp; AcNPV DNA controls with serial dilution scheme for generating absolute standard curves (copies\/µL and pg\/µL).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Sf9 host cell DNA in biopharmaceutical process and final drug substance\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual Autographa californica multiple nucleopolyhedrovirus (AcNPV) DNA in bioprocess samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control and release testing for products produced in Sf9\/baculovirus systems\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV DNA Control (NNA051), lyophilized powder × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001), 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eSf9\u0026amp;AcNPV Primer\u0026amp;Probe MIX (NNC059), 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC067), 150 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001), 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (unused remaining DDB may be stored at 2–8 °C; if cloudy, heat at 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (multiplex quantitative PCR, absolute quantitation via standard curve)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (eyewear, mask, gloves, protective clothing).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free area; use DNase-free, low-retention consumables and tips.\u003c\/p\u003e\r\n \u003cp\u003e• UV-decontaminate work surfaces and equipment for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and spin briefly before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards by serial 10-fold dilutions in DDB as instructed; at least five standard concentrations recommended.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates well, mix and spin down prior to starting qPCR to avoid evaporation and inconsistent Ct values.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC: sample Ct-IPC should be within ±1.0 Ct of NCS; significant increase indicates sample inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC should have Ct ≥ 35.00 or be undetermined; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Reaction Buffer from light.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101101.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: How many reactions does one kit support?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit provides reagents for 100 reactions (stated on the product cover).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and thermal program?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare 30 µL reactions (20 µL qPCR MIX + 10 µL sample). Thermal cycling: Activation 95 °C 10:00; 40 cycles of 95 °C 0:15 and 60 °C 1:00 with fluorescence read during the 60 °C step. Use manual Ct threshold 0.02 as guidance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf sample Ct-IPC is significantly higher than Ct-IPC of NCS (beyond ±1.0 Ct), consider sample dilution, re-extraction, or purification to remove inhibitors. Use recovery of spiked reference standards to assess sample processing.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the assay dynamic ranges and units?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eSf9 standard curve: 300 to 0.003 pg\/µL. AcNPV standard curve: 5.14×10^6 to 5.14×10^1 copies\/µL (equivalent to 20 to 0.0002 pg\/µL). At least five standard concentrations should be used for curve generation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45171362431157,"sku":"1101101","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Sf9_AcNPV_DNA_Quantitation_Kit.jpg?v=1742279477"},{"product_id":"residual-cv-1-dna-quantitation-kit-shentek-n","title":"Residual CV-1 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CV-1 DNA Quantitation Kit is designed to quantitate residual CV-1 host cell DNA across stages of biopharmaceutical production, from in-process samples to final products. The kit uses quantitative PCR (qPCR) to deliver rapid, specific and reliable quantitation at the femtogram (fg) level and includes a CV-1 DNA Control reference standard. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eST0: 3000 pg\/µL; ST1: 300 pg\/µL; ST2: 30 pg\/µL; ST3: 3 pg\/µL; ST4: 0.3 pg\/µL; ST5: 0.03 pg\/µL; ST6: 0.003 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLowest included standard (indicative sensitivity)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e0.003 pg\/µL (3 fg\/µL) — kit supports femtogram-level quantitation; validated LOQ must be determined by laboratory validation\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume: 30 µL (20 µL qPCR mix + 10 µL sample\/diluent)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer: 17 µL; CV-1 Primer \u0026amp; Probe MIX: 3 µL; Total qPCR MIX per reaction: 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C, 10:00 (1 cycle); Denaturation: 95 °C, 00:15 (40 cycles); Annealing\/Extension: 60 °C, 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection settings\u003c\/td\u003e\r\n \u003ctd\u003eRecommended reporter dye: FAM; passive reference: ROX (ABI7500 example). Manual Ct threshold example: 0.02 (Automatic baseline recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 cycles or undetermined (or per laboratory validation); NCS Ct should be larger than mean Ct of lowest standard (or below LOQ if applicable)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components stable up to 24 months at recommended storage conditions (component-specific temperatures listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Claims femtogram-level detection; lowest provided standard 0.003 pg\/µL (3 fg\/µL) allowing very low-level residual DNA quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eqPCR-based absolute quantitation:\u003c\/strong\u003e Absolute quantitation using a supplied CV-1 DNA Control standard curve (six dilution points recommended).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe MIX to streamline assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComprehensive workflow support:\u003c\/strong\u003e Includes guidance for standard curve preparation, plate layout recommendations, qPCR program settings and result analysis instructions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual CV-1 host-cell DNA in final drug substance\/drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host-cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in CV-1 cells\u003c\/li\u003e\r\n \u003cli\u003eProcess development and optimization where host-cell DNA monitoring is required\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 DNA Control (NNA026)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 50 μL × 1 tube\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001)\u003c\/span\u003e\u003cstrong\u003e-20 °C; 850 μL × 2 tubes; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCV-1 Primer \u0026amp; Probe MIX (NNC097)\u003c\/span\u003e\u003cstrong\u003eStore as labeled (provided 300 μL × 1 tube); follow kit storage instructions\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C during use); 1.5 mL × 3 tubes\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation\u003c\/span\u003e\u003cstrong\u003eUser Guide and instructions included\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for human diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Use appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces and pipettes with UV (30 minutes) and 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice (2–8 °C), vortex and spin briefly before use; protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve fresh using serial dilutions; include at least five concentrations for curve fitting.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates with appropriate film, mix and spin briefly before placing on instrument.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC Ct ≥35 cycles or undetermined (or per lab validation); NCS Ct must be \u0026gt; mean Ct of lowest standard or below validated LOQ.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific program and detector settings must be configured per instrument\/software documentation (ABI7500 example provided).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101105.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity or lowest detectable concentration?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides a lowest standard of 0.003 pg\/µL (3 fg\/µL) and is designed to support femtogram-level quantitation. The validated limit of quantitation (LOQ) should be determined by laboratory validation and may be lower or higher depending on sample matrix and extraction recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How should reagents be stored and handled?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at recommended temperatures (major components at -20 °C). Thaw reagents on ice (2–8 °C), vortex and spin briefly before use. Protect light-sensitive components (e.g., qPCR Reaction Buffer) from light. Follow MSDS guidance and use DNase-free consumables.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What acceptance criteria should I use for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should have Ct ≥35 cycles or be undetermined (or per laboratory validation). The NCS Ct should be larger than the mean Ct of the lowest concentration in the standard curve or below the validated LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Which instruments are supported and what detector settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, LineGene 9600). In the ABI7500 example, create a FAM detector (reporter FAM), set ROX as passive reference, use Manual Ct threshold 0.02 and Automatic baseline; thermal cycling: 95 °C activation 10:00; 40 cycles of 95 °C 00:15 and 60 °C 1:00 (read fluorescence during anneal\/extension). Adjust settings per instrument and software version.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45187376840885,"sku":"1101105","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CV-1_DNA_Quantitation_Kit.webp?v=1742279215"},{"product_id":"residual-hi5-acnpv-dna-quantitation-kit-shentek®","title":"Residual Hi5 \u0026 AcNPV DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n 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8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Hi5\u0026amp;AcNPV DNA Quantitation Kit is designed to quantify residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples (in-process and final products). The kit uses a multiplex quantitative PCR (qPCR) assay with target-specific primer\/probe mixes for Hi5 DNA and AcNPV DNA plus an Internal Positive Control (IPC) to monitor reaction performance and potential inhibition. The assay supports absolute quantitation using a supplied lyophilized DNA control and DDB-based serial dilutions. The kit provides reagents for 100 reactions and is compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual Hi5 and AcNPV host-cell DNA in biopharmaceutical samples across process stages\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eMultiplex quantitative PCR (qPCR) with three detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 μL total per well (20 μL qPCR mix + 10 μL sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; Hi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00; 40 cycles of 95 °C 00:15 and 60 °C 01:00 (fluorescence read at 60 °C)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eHi5 DNA standard curve (mass)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points: 30 pg\/μL (ST1), 3 pg\/μL (ST2), 0.3 pg\/μL (ST3), 0.03 pg\/μL (ST4), 0.003 pg\/μL (ST5)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcNPV DNA standard curve\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eStandard points (copies\/μL and pg\/μL): ST1 5.14×10^5 copies\/μL (2 pg\/μL), ST2 5.14×10^4 (0.2 pg\/μL), ST3 5.14×10^3 (0.02 pg\/μL), ST4 5.14×10^2 (0.002 pg\/μL), ST5 5.14×10^1 (0.0002 pg\/μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity claim\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level is supported by the provided standard curve down to 0.003 pg\/μL for Hi5 and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eIPC Ct of sample vs NCS should be within ±1.0 Ct; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger (i.e., later) than mean Ct of lowest standard; NCS should show VIC amplification\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input volume\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample (per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eComponents stable up to 24 months when stored as directed\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided quantity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents sufficient for 100 reactions (kit contains reagents sized as listed in kit contents)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOD \/ LOQ\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eValidated LOQ is referenced in method validation; the user should determine LOQ during method validation. If LOQ \u0026lt; lowest standard, NCS value should be less than LOQ\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMultiplex qPCR format:\u003c\/strong\u003e Simultaneous detection of Hi5 DNA, AcNPV DNA and IPC in a single reaction (FAM, CY5, VIC channels)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Supports quantitation down to femtogram-level concentrations using supplied standards (mass and copy-number options for AcNPV)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify inhibited samples\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer\u0026amp;Probe MIX and IPC MIX reduce assay setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common qPCR instruments:\u003c\/strong\u003e Validated on multiple real-time PCR platforms (SHENTEK-96S, ABI 7500, Bio-Rad CFX96, FQD-96A)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual Hi5 host-cell DNA in biopharmaceutical process development and quality control\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual AcNPV DNA (baculovirus) in upstream and downstream process samples\u003c\/li\u003e\r\n \u003cli\u003eIn-process monitoring of host DNA clearance during purification workflows\u003c\/li\u003e\r\n \u003cli\u003eRelease testing support and method development for biologics produced in Hi5\/AcNPV systems\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV DNA Control (lyophilized powder) ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHi5\u0026amp;AcNPV Primer\u0026amp;Probe MIX 300 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer 850 μL ×2 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX 150 μL ×1 tube\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) 1.5 mL ×3 tubes\u003c\/span\u003e\u003cstrong\u003e–20 °C (after opening\/for use, store remaining DDB at 2–8 °C)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (gloves, mask, protective eyewear, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, micropipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents on ice or at 2–8 °C, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Follow plate layout recommendations and include at least five standard curve points, NTC and NCS in each run.\u003c\/p\u003e\r\n \u003cp\u003e• IPC acceptance: Ct(sample IPC) must be within ±1.0 Ct of Ct(NCS) — higher IPC Ct indicates potential inhibition.\u003c\/p\u003e\r\n \u003cp\u003e• NTC acceptance: Ct ≥ 35 or undetermined; NTC should not show target amplification.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Validated LOQ must be established during method validation; if LOQ is below the lowest standard, ensure NCS value is below LOQ.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101102.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the minimum detectable concentration for Hi5 and AcNPV using this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe provided standard curve supports quantitation down to 0.003 pg\/μL for Hi5 DNA and 0.0002 pg\/μL (5.14×10^1 copies\/μL) for AcNPV DNA. Users should perform method validation to determine assay LOD\/LOQ for their matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How much sample should be added per reaction?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eAdd 10 μL of purified test sample per 30 μL reaction (20 μL qPCR mix + 10 μL sample).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What are the instrument settings for the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThermal profile: Activation 95 °C for 10:00; 40 cycles of 95 °C for 00:15 and 60 °C for 01:00 with fluorescence read at 60 °C. Set reaction volume to 30 μL. Configure detectors: Hi5-DNA (FAM), AcNPV-DNA (CY5), IPC (VIC); ROX as passive reference.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if IPC indicates inhibition?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC Ct is significantly higher than NCS IPC Ct (outside ±1.0 Ct), suspect inhibition. Consider diluting the sample, re-extracting with improved purification, or including spike-recovery controls to assess sample recovery.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173391065269,"sku":"1101102","price":2575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Hi5_AcNPV_DNA_Quantitation_Kit.png?v=1742279579"},{"product_id":"residual-human-dna-quantitation-kit-shentek®","title":"Residual Human DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual Human DNA Quantitation Kit (2G) is a duplex quantitative PCR (qPCR) kit for quantitation of residual human host-cell DNA in biopharmaceutical samples from in-process to final products. The assay achieves femtogram-level sensitivity and includes an Internal Positive Control (IPC) in the Human Primer \u0026amp; Probe MIX to monitor PCR performance. The kit contains a Human DNA Control for standard curve generation and ready-to-use qPCR Master MIX and Primer\u0026amp;Probe MIX. Intended for research use only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual human host-cell DNA in biopharmaceutical samples (in-process to final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd\u003eDuplex quantitative PCR (qPCR) with Human-DNA detector (FAM) and IPC (VIC)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation at femtogram (fg) level; NTC detection value acceptance ≤ 6.00 fg\/μL (user may set criteria by method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (example)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/μL; ST2 = 30 pg\/μL; ST3 = 3 pg\/μL; ST4 = 0.3 pg\/μL; ST5 = 0.03 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTypical linear range (based on standards)\u003c\/td\u003e\r\n \u003ctd\u003e300 pg\/μL to 0.03 pg\/μL (example standard series provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis thresholds (example)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct thresholds: Human-DNA = 0.05; IPC = 0.1; Automatic baseline\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControl acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery rate 50%–150%; NCS Ct should be larger than mean Ct of lowest standard and show IPC amplification; NTC detection ≤ 6.00 fg\/μL and IPC amplification normal\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePack size \/ yield\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions (product no. 1101108)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored at appropriate conditions for up to 24 months (see component labels)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation at femtogram (fg) level\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDuplex assay with IPC:\u003c\/strong\u003e Includes Internal Positive Control in the Primer\u0026amp;Probe MIX to monitor PCR performance and detect inhibition\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Supplied qPCR Master MIX and Primer\u0026amp;Probe MIX reduce setup time and variability\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eBroad instrument compatibility:\u003c\/strong\u003e Validated on multiple real-time PCR systems (SHENTEK-96S, ABI 7500, CFX96, LightCycler 480)\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eWorkflow convenience:\u003c\/strong\u003e Includes Human DNA Control and DNA Dilution Buffer with recommended serial dilution scheme for standard curve generation\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual human host-cell DNA in biopharmaceutical products (in-process and final product)\u003c\/li\u003e\r\n \u003cli\u003eProcess monitoring and release testing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host DNA\u003c\/li\u003e\r\n \u003cli\u003eComparative testing of extraction methods (use with Extraction Reference Control, ERC)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman DNA Control (NNA003) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Master MIX (NNB023) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eHuman Primer \u0026amp; Probe MIX (includes IPC) (NNC130) 500 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eReagents for 100 reactions (kit pack information)\u003c\/span\u003e\u003cstrong\u003e-20 °C (components stable up to 24 months)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination. UV decontaminate work surfaces and pipettes prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect qPCR Master MIX from light.\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, and ERC. Run standards (at least five concentrations) to build a standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50%–150%; NTC ≤ 6.00 fg\/μL (or criteria defined during method validation); NCS Ct larger than mean Ct of lowest standard and shows IPC amplification.\u003c\/p\u003e\r\n \u003cp\u003e• qPCR analysis parameters (e.g., thresholds, baseline) should be configured for the specific instrument and software version.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101108.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the lowest concentration this kit can reliably detect?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for femtogram-level quantitation. Example standard points include down to 0.03 pg\/μL (30 fg\/μL). The kit documentation states NTC detection acceptance ≤ 6.00 fg\/μL; true LOD\/LOQ should be determined by the user's method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Roche LightCycler 480. It is compatible with other real-time PCR instruments that support dual-channel detection (FAM and VIC), but instrument-specific setup is required.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How many reactions are included and how should reagents be stored?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. Store components at -20 °C and protect the qPCR Master MIX from light. Components are stable up to 24 months—confirm expiration on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should I run and what are acceptance criteria?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eRun a standard curve (minimum five points), Non-Template Control (NTC), Negative Control Sample (NCS), and Extraction Reference Control (ERC). ERC recovery should be between 50% and 150%. NTC detection should be ≤ 6.00 fg\/μL (or per user validation). NCS Ct should be larger than the mean Ct of the lowest standard and show IPC amplification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173398765749,"sku":"1101108","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Human_DNA_Quantitation_Kit.png?v=1742279705"},{"product_id":"residual-bhk-dna-quantitation-kit-shentek®","title":"Residual BHK DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual BHK DNA Quantitation Kit is designed to quantitate residual BHK host cell DNA in different stages of biopharmaceutical products, from in‑process samples to final products. The kit utilizes fluorescent quantitative PCR (qPCR) to perform a rapid, specific, and reliable quantitation assay at the femtogram (fg) level. The kit includes a BHK DNA Control and reagents for preparing standard curves and performing absolute quantitation. For DNA extraction, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical samples (in‑process to final product)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay format\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eAbsolute quantitation by fluorescent qPCR (standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetects at the femtogram (fg) level (laboratory validation recommended)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e3000 pg\/µL, 300 pg\/µL, 30 pg\/µL, 3 pg\/µL, 0.3 pg\/µL, 0.03 pg\/µL (ST0–ST5 as prepared per protocol)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended reaction volume\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 µL + BHK Primer\u0026amp;Probe MIX 3 µL → 20 µL qPCR mix; add 10 µL sample\/standard to reach 30 µL total\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling program\u003c\/td\u003e\r\n \u003ctd\u003eActivation 95 °C 10:00 (1 cycle); Denaturation 95 °C 00:15 (40 cycles); Annealing\/Extension 60 °C 1:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence settings\u003c\/td\u003e\r\n \u003ctd\u003eReporter dye: FAM; Passive reference: ROX; Quencher: none (per setup instructions)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eControls and acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eNTC Ct should be ≥ 35 cycles or undetermined; NCS Ct should be larger than mean Ct of the lowest standard (or larger than LOQ Ct if LOQ is below lowest standard); ERC recovery rate should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components stored at -20 °C; stable up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProvided reaction count\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents supplied for 100 reactions (kit format)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Capable of quantitating residual BHK DNA at the femtogram (fg) level when properly validated.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes BHK DNA Control and standard curve workflow for absolute quantitation of target DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Contains qPCR Reaction Buffer and Primer \u0026amp; Probe MIX optimized for the assay to reduce setup variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatible with common instruments:\u003c\/strong\u003e Validated for use on SHENTEK‑96S, ABI 7500, Roche LightCycler 480 II and other real‑time PCR systems (instrument settings may vary).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Intended for testing residual host cell DNA across in‑process samples to final product stages and for assay validation workflows.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual BHK host cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn‑process monitoring of host cell DNA levels\u003c\/li\u003e\r\n \u003cli\u003eFinal product release testing for residual DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for host cell DNA quantitation\u003c\/li\u003e\r\n \u003cli\u003eQuality control and stability studies involving host cell DNA measurements\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK DNA Control (Part No. NNA029) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (Part No. NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBHK Primer \u0026amp; Probe MIX (Part No. NNC076) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (Part No. NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only. Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow all handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase‑free, low retention consumables and clean work area (UV irradiation and 75% ethanol decontamination recommended).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice, vortex and briefly spin before use; protect light‑sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration date on the label.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard points for the standard curve and perform triplicates for samples and controls where recommended.\u003c\/p\u003e\r\n \u003cp\u003e• ERC recovery rates should be between 50% and 150%; investigate deviations.\u003c\/p\u003e\r\n \u003cp\u003e• Set instrument analysis parameters (thresholds, baseline, detector settings) according to instrument\/software and laboratory validation.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101116.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to enable quantitation at the femtogram (fg) level. The practical limit of detection (LOD) and limit of quantification (LOQ) should be established by laboratory validation. The provided standard curve includes concentrations down to 0.03 pg\/µL (30 fg\/µL as prepared).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What instruments are compatible with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is compatible with common real‑time PCR instruments. The user guide provides example settings for SHENTEK‑96S, ABI 7500 (SDS v1.4), and Roche LightCycler 480 II. Adjust instrument and software parameters as needed for other platforms and validate performance.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThaw the BHK DNA Control and DNA Dilution Buffer at 2–8 °C, dilute the control to 3000 pg\/µL (ST0), then perform serial 1:10 dilutions to generate ST1–ST5 (300, 30, 3, 0.3, 0.03 pg\/µL). Include at least five concentration points and run standards in triplicate.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What should I do if the NTC or NCS show amplification?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf NTC shows amplification (Ct \u0026lt; 35), verify reagent and plate handling to identify contamination sources, replace consumables, re‑prepare reagents, decontaminate work surfaces and equipment (UV\/ethanol), and repeat the assay. If NCS Ct is not larger than the lowest standard Ct or LOQ Ct, review extraction and dilution procedures and revalidate the LOQ.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173411610805,"sku":"1101116","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_BHK_DNA_Quantitation_Kit.png?v=1742279915"},{"product_id":"residual-e1a-dna-quantitation-kit-shentek®","title":"Residual E1A DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: 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repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1A DNA Quantitation Kit is designed for the quantitation of residual E1A DNA derived from host cells (e.g., HEK293 and 293T) across different stages of biopharmaceutical manufacture, from in-process samples to final products. The kit uses fluorescent quantitative PCR (real-time qPCR) for rapid, specific and reliable absolute quantitation. An Internal Positive Control (IPC) is included to monitor PCR performance and potential inhibition. The kit provides both E1A linear DNA Control and E1A non-linear DNA Control to accommodate different user needs. Recommended sample extraction procedures are provided in the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e4.97 × 10^8 copies\/μL (stock) with serial 10-fold dilutions down to 4.97 × 10^1 copies\/μL (ST1–ST6 covering 4.97 × 10^6 to 4.97 × 10^1 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical standard range (non-linear DNA control)\u003c\/td\u003e\r\n \u003ctd\u003e2.98 × 10^7 copies\/μL (stock) with serial 10-fold dilutions down to 2.98 × 10^2 copies\/μL (ST0–ST5 covering 2.98 × 10^6 to 2.98 × 10^2 in standard curve steps)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL; E1A Primer \u0026amp; Probe MIX 2.8 μL; IPC MIX 1.3 μL (total 20 μL qPCR MIX)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 μL purified test sample or purified NCS per reaction; recommended starting material for extraction: 100 μL sample\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorescence detection dyes\u003c\/td\u003e\r\n \u003ctd\u003eE1A-DNA detector: CY5; IPC detector: VIC; Passive reference: ROX\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for both E1A-DNA and IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt value of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; significant shift indicates possible inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLOQ \/ LOD\u003c\/td\u003e\r\n \u003ctd\u003eLimit of quantification (LOQ) not specified in kit documentation; users should determine proven LOQ during method validation. If proven LOQ is lower than lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended replicates and standard points\u003c\/td\u003e\r\n \u003ctd\u003eTriplicate measurements recommended; at least five concentration points recommended for standard curve (six when using linear DNA control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eValidated instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500 (SDS v1.4 example), Bio-Rad CFX96 (other compatible real-time PCR systems may be used)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C (stable for 24 months)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eSpecific and sensitive absolute quantitation:\u003c\/strong\u003e Fluorescent qPCR assay for quantitative detection of residual E1A DNA with serial standards to create an absolute standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal Positive Control (IPC):\u003c\/strong\u003e IPC included to monitor PCR performance and identify potential sample inhibition; IPC results used to assess recovery when spiked samples are tested.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eTwo control formats provided:\u003c\/strong\u003e Both E1A linear DNA Control (lyophilized) and E1A non-linear DNA Control are supplied so users can choose according to validation needs.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX supplied in ready-to-use formats to simplify setup; protect light-sensitive reagents from light.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on common real-time PCR platforms (SHENTEK-96S, ABI 7500, CFX96); thermal and detector settings provided for instrument configuration.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1A DNA from host cells (e.g., HEK293, 293T) in biopharmaceutical development and manufacturing\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing and final product testing for residual host-cell E1A DNA\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and QC workflows requiring absolute quantitation of E1A sequences\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A linear DNA Control (lyophilized powder × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A non-linear DNA Control (50 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1A Primer \u0026amp; Probe MIX (300 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube)\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes)\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow laboratory safety procedures; wear appropriate PPE (gloves, eyewear, mask, lab coat).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol before setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use. Protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at -20 °C and check expiration dates. Do not use if reagents show precipitate or cloudiness (heat DDB at 37 °C to clarify if necessary).\u003c\/p\u003e\r\n \u003cp\u003e• Include appropriate controls: NTC, NCS, standards and IPC. Use triplicates for samples and standards for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• Instrument-specific settings should be adapted to the user's instrument and software version; follow provided detector\/dye assignments and thermal profile as guidance.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101109.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What sample types can be tested with this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for quantitation of residual E1A DNA from host cell-derived samples (for example, HEK293 and 293T) and is applicable to samples collected during biopharmaceutical manufacturing (in-process and final product). Samples must be purified prior to qPCR according to the recommended extraction workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How is the standard curve prepared and what dynamic range is covered?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStandards are prepared by serial 10-fold dilutions of the provided DNA controls. The linear DNA control stock is 4.97 × 10^8 copies\/μL (diluted down to 4.97 × 10^1 copies\/μL across ST1–ST6); the non-linear control stock is 2.98 × 10^7 copies\/μL (diluted down to 2.98 × 10^2 copies\/μL across ST0–ST5). At least five standard points (six for linear control) are recommended.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What quality criteria should I use to accept a run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eNTC should be undetermined or have Ct ≥ 35. IPC Ct for sample should be within ±1.0 Ct of IPC Ct for NCS; significant shifts indicate inhibition. Verify standard curve slope, intercept and R^2 before reporting sample quantities.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: Is the limit of quantification (LOQ) provided?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eA numeric LOQ is not specified in the kit documentation. Users should determine the proven LOQ during method validation for their specific matrix and instrument. If proven LOQ is below the lowest standard, Ct(NCS) should be larger than Ct(LOQ).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173426094261,"sku":"1101109","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1A_DNA_Quantitation_Kit.png?v=1742280136"},{"product_id":"residual-e1b-dna-quantitation-kit-shentek®","title":"Residual E1B DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n 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0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual E1B DNA Quantitation Kit is designed for absolute quantitation of residual E1B DNA derived from host cells (e.g., HEK293 and derivative cell lines such as 293T and 293F) in various stages of biopharmaceutical manufacturing (from in-process samples to final products). The kit uses a fluorescent quantitative PCR (qPCR) assay with an internal positive control (IPC) to monitor PCR performance and includes an E1B linear DNA Control for preparation of standard curves and for detection of linearized samples. The kit is supplied for 100 reactions and is intended for research use only. For sample extraction procedures, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended reactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection format\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by fluorescent qPCR (FAM-labeled target, VIC-labeled IPC, ROX passive reference)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentration range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eST1 to ST6: 4.34 × 10^6 to 4.34 × 10^1 copies\/μL (prepared from E1B linear DNA Control; original ST = 4.34 × 10^8 copies\/μL before serial dilution)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR MIX + 10 μL sample or control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 μL, E1B Primer\u0026amp;Probe MIX 2.8 μL, IPC MIX 1.3 μL (total qPCR MIX = 20 μL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00; Denaturation: 95 °C 0:15; Annealing\/Extension: 60 °C 1:00; 40 cycles; fluorescence read during annealing\/extension\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument settings \/ analysis\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with threshold = 0.02 for both E1B-DNA and IPC; Automatic baseline; Standard curve (absolute quantitation) analysis\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control acceptance\u003c\/td\u003e\r\n \u003ctd\u003eCt of NTC should be ≥ 35.00 cycles or undetermined\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIPC acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt(IPC) of sample should be within ±1.0 Ct of Ct(IPC) of NCS; a significantly higher Ct(IPC) indicates potential PCR inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input for preparation\u003c\/td\u003e\r\n \u003ctd\u003e100 μL test sample (or 100 μL DDB for negative control sample preparation prior to extraction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under stated conditions for up to 24 months; check label expiration date\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eLimit of quantification \/ limit of detection\u003c\/td\u003e\r\n \u003ctd\u003eNot numerically specified in the manual. Users should establish LOD\/LOQ by validation; standard curve lowest point in user protocol is 4.34 × 10^1 copies\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh specificity and sensitivity:\u003c\/strong\u003e Fluorescent qPCR assay with sequence-specific primers and probe for E1B enabling specific detection of residual E1B DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e Included IPC MIX monitors PCR performance and helps identify sample inhibition.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use components:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer, Primer \u0026amp; Probe MIX and IPC MIX minimize setup errors and speed assay setup.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eE1B linear DNA Control:\u003c\/strong\u003e Supplied lyophilized linear DNA control for preparation of serial dilutions and construction of an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Validated on multiple real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, qTOWER3G, LightCycler480).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eFor residual host cell DNA testing:\u003c\/strong\u003e Designed specifically to quantitate residual E1B DNA from HEK293 and derivative cell lines in biopharmaceutical samples.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual E1B DNA in biopharmaceutical products (in-process and final products)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for products produced in HEK293 and derivative cell lines (e.g., 293T, 293F)\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and assay development for residual host cell DNA analysis\u003c\/li\u003e\r\n \u003cli\u003eAssessment of DNA clearance and downstream process effectiveness\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B linear DNA Control (lyophilized powder × 1 tube) — Part No. NNA014\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (850 μL × 2 tubes) — Part No. NNB002\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eE1B Primer \u0026amp; Probe MIX (300 μL × 1 tube) — Part No. NNC034\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (150 μL × 1 tube) — Part No. NNC066\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (1.5 mL × 3 tubes) — Part No. NND001\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR (fluorescent quantitative PCR, absolute quantitation)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For research use only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free consumables and low-retention tips to avoid contamination and loss.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect light-sensitive reagents (qPCR Reaction Buffer, Primer\u0026amp;Probe MIX, IPC MIX) from light.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate work surfaces, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup to reduce contamination risk.\u003c\/p\u003e\r\n \u003cp\u003e• Ensure lyophilized E1B linear DNA Control is fully dissolved (add 55 μL ddH2O and mix as instructed).\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations and run samples in triplicate for reliable quantitation.\u003c\/p\u003e\r\n \u003cp\u003e• NTC Ct should be ≥ 35 or undetermined; check IPC Ct to assess inhibition (sample IPC Ct must be within ±1.0 Ct of NCS IPC Ct).\u003c\/p\u003e\r\n \u003cp\u003e• Establish assay-specific LOD\/LOQ during method validation; lowest manual standard = 4.34 × 10^1 copies\/μL.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101110.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses fluorescent quantitative PCR (qPCR) for absolute quantitation of E1B DNA (FAM-labeled target, VIC-labeled IPC, ROX passive reference).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are provided per kit and what is the recommended reaction volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 100 reactions. The recommended total reaction volume is 30 μL (20 μL qPCR MIX + 10 μL sample\/control).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What is the standard curve range included in the user protocol and is LOQ specified?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe protocol prepares a standard curve with concentrations from 4.34 × 10^6 down to 4.34 × 10^1 copies\/μL (ST1–ST6). A numeric LOQ\/LOD is not specified in the manual; users should determine assay-specific LOD\/LOQ during validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I interpret IPC and controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIPC (internal positive control) monitors PCR performance and sample inhibition. The Ct(IPC) of a sample should be within ±1.0 Ct of the Ct(IPC) of the NCS. The NTC should have Ct ≥ 35 or be undetermined. A significantly higher Ct(IPC) in a sample indicates possible inhibition; consider dilution or purification.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173461713077,"sku":"1101110","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_E1B_DNA_Quantitation_Kit.png?v=1742280232"},{"product_id":"residual-pg13-dna-quantitation-kit-shentek®","title":"Residual PG13 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003e\u003c\/p\u003e\n\u003cp\u003eSHENTEK® Residual PG13 DNA Quantitation Kit is designed to quantify residual PG13 host cell DNA in biopharmaceutical samples at different stages (in-process through final product). The kit uses quantitative PCR (qPCR) to provide a rapid, specific and reliable quantitation assay with sensitivity at the femtogram (fg) level. The kit includes a PG13 DNA Control as a reference standard and reagents to prepare standard curves and perform absolute quantitation. For extraction recommendations, refer to the SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide. The kit is supplied for research use only and provides reagents sufficient for 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eQuantitative PCR (qPCR) using FAM-labeled probe\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to the femtogram (fg) level (analytical sensitivity stated as fg-level in kit documentation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (pg\/μL)\u003c\/td\u003e\r\n \u003ctd\u003e3000 (ST0 stock) and working standards ST1=300, ST2=30, ST3=3, ST4=0.3, ST5=0.03, ST6=0.003 pg\/μL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDynamic range (demonstrated by standards)\u003c\/td\u003e\r\n \u003ctd\u003eApproximately 300 to 0.003 pg\/μL (prepared standard series provides \u0026gt;5 orders of magnitude coverage from working standards)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 μL total reaction volume (20 μL qPCR mix + 10 μL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003ePer-reaction reagent volumes (qPCR MIX)\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 17 μL + PG13 Primer\u0026amp;Probe MIX 3 μL (total 20 μL qPCR MIX per reaction)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR thermal cycling\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15 (40 cycles); Annealing\/Extension: 60 °C 01:00 (fluorescence read during this step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S, ABI 7500, Roche LightCycler 480 II (and other compatible real-time PCR systems supporting FAM detection and absolute quantitation mode)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct; Threshold = 0.02 (PG13-DNA detector); Auto baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAcceptance criteria \/ QC\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eERC recovery: 50%–150%; NTC Ct should be ≥35 or undetermined; NCS Ct should be larger than mean Ct of lowest standard (or \u0026lt; QL when QL \u0026lt; lowest standard); verify slope\/intercept\/R² for standard curve\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eShelf life \/ stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 °C storage; kit components stable for up to 24 months (check label expiration date)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ throughput\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Analytical sensitivity at the femtogram (fg) level for detection of trace residual PG13 DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes PG13 DNA Control and a serial dilution standard curve to perform absolute quantitation (standard curve method).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e qPCR Reaction Buffer and PG13 Primer \u0026amp; Probe mix provided for streamlined plate setup and consistent reactions.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow guidance:\u003c\/strong\u003e Detailed instructions for serial dilutions, controls (ERC, PCS, NCS, NTC), qPCR mix preparation and recommended analysis parameters.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCompatibility:\u003c\/strong\u003e Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500, LightCycler 480 II).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eResearch use supply:\u003c\/strong\u003e Supplied for Research Use Only (RUO) and includes reagents sufficient for 100 reactions.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual PG13 host cell DNA in biopharmaceutical process intermediates\u003c\/li\u003e\r\n \u003cli\u003eQuantitation of residual PG13 DNA in final drug substance and drug product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA assays targeting PG13 cell line\u003c\/li\u003e\r\n \u003cli\u003eIn-process quality control monitoring of host cell DNA removal\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 DNA Control (NNA049) 50 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 μL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePG13 Primer \u0026amp; Probe MIX (NNC101) 300 μL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (store unused DDB at 2–8 °C for working use as directed)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or clinical use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling and disposal instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Prevent nucleic acid contamination: irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standards and controls carefully; include at least five concentrations in the standard curve.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix and centrifuge briefly before running the qPCR.\u003c\/p\u003e\r\n \u003cp\u003e• Follow recommended analysis settings (Manual Ct, Threshold 0.02, Auto Baseline) or validate settings per instrument\/software.\u003c\/p\u003e\r\n \u003cp\u003e• QC acceptance: ERC recovery 50%–150%; NTC should be ≥35 cycles or undetermined; NCS Ct must be higher than the lowest standard Ct (or meet QL criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101123.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the detection method used by this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit uses quantitative PCR (qPCR) with a FAM-labeled probe for absolute quantitation using a standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What sensitivity can I expect from the assay?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit documentation reports sensitivity at the femtogram (fg) level. Working standard series are provided down to 0.003 pg\/μL (3 fg\/μL), but laboratories should validate the limit of quantitation (QL) in their own workflow.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should samples and reagents be prepared and loaded?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003ePrepare qPCR MIX (17 μL qPCR Reaction Buffer + 3 μL Primer\u0026amp;Probe MIX per reaction), aliquot 20 μL per well and add 10 μL of sample or standard (total 30 μL reaction). Include NTC, NCS, PCS and ERC controls and run standards in triplicate. Seal plate, mix and centrifuge briefly before starting the run.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What are the acceptance criteria for controls?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eERC recovery should be between 50% and 150%. NTC should be ≥35 cycles or undetermined. NCS Ct should be larger than the mean Ct of the lowest standard or meet QL criteria determined in validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45173516992693,"sku":"1101123","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_PG13_DNA_Quantitation_Kit.png?v=1742289744"},{"product_id":"residual-mrc-5-dna-quantitation-kit-shentek®","title":"Residual MRC-5 DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual MRC-5 DNA Quantitation Kit is a qPCR-based assay for quantitation of residual MRC-5 host cell DNA in biopharmaceutical samples at in-process and final product stages. The kit enables rapid, specific, and reliable absolute quantitation down to the femtogram (fg) level using an included MRC-5 DNA Control as reference standard. The kit is supplied as reagents for 100 reactions (Product No.: 1101124) and is intended for research use only. For sample extraction guidance, refer to the corresponding SHENTEK® Residual Host Cell DNA Sample Preparation Kit User Guide (Product No. 1104191). Compatible with common real-time PCR instruments (e.g., SHENTEK-96S, ABI 7500).\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd\u003eQuantitation of residual MRC-5 host cell DNA in biopharmaceutical samples (in-process and final products)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection method\u003c\/td\u003e\r\n \u003ctd\u003eAbsolute quantitation by qPCR (FAM reporter)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity \/ analytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level; lowest standard used 0.03 pg\/µL (30 fg\/µL).\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations\u003c\/td\u003e\r\n \u003ctd\u003eStandard points: ST1 = 300 pg\/µL, ST2 = 30 pg\/µL, ST3 = 3 pg\/µL, ST4 = 0.3 pg\/µL, ST5 = 0.03 pg\/µL (prepare from ST0 = 3000 pg\/µL)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC acceptance criteria\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection value of NTC should be ≤ 6.00 fg\/µL (or laboratory-specific criteria after method validation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery acceptance (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery should be between 50% and 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR mix + 10 µL template\/sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR reaction mix per test\u003c\/td\u003e\r\n \u003ctd\u003ePer reaction: qPCR Reaction Buffer 17 µL + MRC-5 Primer \u0026amp; Probe MIX 3 µL (prepare 20 µL qPCR MIX then add 10 µL sample\/control)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95°C 10:00 (1 cycle); Denaturation: 95°C 0:15 (40 cycles); Annealing\/extension and read: 60°C 1:00 (40 cycles)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInstrument compatibility\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 example provided); compatible with standard real-time PCR instruments\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit format and size\u003c\/td\u003e\r\n \u003ctd\u003eReagents for 100 reactions; Product No. 1101124; Version A\/0\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd\u003eKit components can be stored under appropriate conditions for up to 24 months (individual components stored at -20 °C as labeled)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eResult reporting units\u003c\/td\u003e\r\n \u003ctd\u003eMean Quantity reported in pg\/µL (results exportable from instrument software)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eQuality control \/ acceptance\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard; NTC detection value ≤ 6.00 fg\/µL; ERC recovery 50–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to the femtogram level (lowest standard 0.03 pg\/µL = 30 fg\/µL) enabling detection of trace residual host-cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes MRC-5 DNA Control for construction of a standard curve and absolute quantitation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR reagents:\u003c\/strong\u003e Pre-formulated qPCR Reaction Buffer and Primer \u0026amp; Probe mix simplify setup and reduce preparation variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated workflows and example settings provided for common real-time PCR platforms (ABI 7500, SHENTEK-96S).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eDesigned for biopharma QC:\u003c\/strong\u003e Appropriate for in-process and final product testing, and for method validation and routine QC of residual host cell DNA.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual MRC-5 host-cell DNA in biopharmaceutical products\u003c\/li\u003e\r\n \u003cli\u003eIn-process sample testing during downstream processing\u003c\/li\u003e\r\n \u003cli\u003eFinal product QC testing for host-cell DNA content\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and recovery assessment (using ERC samples)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 DNA Control (NNA062) 50 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB001) 850 µL × 2 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eMRC-5 Primer \u0026amp; Probe MIX (NNC118) 300 µL × 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 °C (remaining unused DDB store at 2–8 °C after thaw; if cloudy, heat to 37 °C until clear)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Read Material Safety Data Sheets (MSDS) before use.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE: protective eyewear, mask, lab coat and gloves.\u003c\/p\u003e\r\n \u003cp\u003e• Prevent contamination: irradiate bench, pipettes and tubes with UV for 30 minutes and disinfect with 75% ethanol prior to setup.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2–8 °C or on ice; vortex and briefly spin down before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use RNase\/DNase-free consumables and low-retention tips; follow sterile technique.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare standard curve with at least five concentration points; perform method validation to select sample dilutions.\u003c\/p\u003e\r\n \u003cp\u003e• Seal plates properly, mix on microplate shaker and briefly centrifuge before running.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptance criteria: ERC recovery 50–150%; NCS Ct should be larger than the mean Ct of the lowest standard; NTC signal ≤ 6.00 fg\/µL (or lab-defined criteria).\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101124.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the analytical sensitivity (limit of detection) of the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed to detect residual MRC-5 DNA at the femtogram (fg) level. The lowest standard provided is 0.03 pg\/µL (30 fg\/µL). The NTC acceptance criterion is ≤ 6.00 fg\/µL; final LOD should be established during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: How many reactions are supplied in a single kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThis kit supplies reagents for 100 reactions (Product No. 1101124).\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: What instrument settings should I use?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings for ABI 7500 (SDS v1.4) are provided: 30 µL reaction volume; Activation 95°C 10:00; 40 cycles of 95°C 0:15 and 60°C 1:00 with signal read at 60°C. If using other instruments, adapt thermal profile and settings according to the instrument documentation and validate accordingly.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How should I store kit components and how long are they stable?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at -20 °C as labeled. The kit components can be stored under appropriate conditions for up to 24 months. After thawing, store unused DNA Dilution Buffer (DDB) at 2–8 °C; if it becomes cloudy, incubate at 37 °C until clear.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175198253237,"sku":"1101124","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_MRC-5_DNA_Quantitation_Kit.png?v=1742289936"},{"product_id":"residual-bovine-dna-quantitation-kit-shentek®","title":"Residual Bovine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e-20 b0C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK4 Residual Bovine DNA Quantitation Kit is designed to quantitate residual bovine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The assay provides rapid, specific and reliable quantitation down to the femtogram (fg) level. The kit includes bovine DNA control, primer \u0026amp; probe mix, internal positive control (IPC) mix, qPCR reaction buffer and DNA dilution buffer. Compatible with common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500). For sample extraction recommendations refer to the SHENTEK Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). For Research Use Only.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eProduct Number\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e1101112\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eKit size \/ Throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical principle\u003c\/td\u003e\r\n \u003ctd\u003eFluorescent quantitative PCR (absolute quantitation, standard curve)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalytical sensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eSensitive to femtogram (fg) level (kit description states fg-level sensitivity)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve \/ Calibration range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003ePrepared standards from 3000 pg\/bcl down to 0.03 pg\/bcl (ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 bL total reaction volume (20 bL qPCR MIX + 10 bL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 bL, Bovine primer \u0026amp; probe MIX 2.8 bL, IPC MIX 1.3 bL (total 20 bL); add 10 bL sample or standard\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eUNG (optional) 25 b0C 10:00; Activation 95 b0C 10:00; 40 cycles of Denaturation 95 b0C 00:15 and Annealing\/Extension 63 b0C 00:40 (fluorescence read during 63 b0C step)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria (IPC)\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct(IPC) of sample should be within b10.0 Ct of NCS (if significantly higher, sample inhibition suspected)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eExtraction Recovery acceptance\u003c\/td\u003e\r\n \u003ctd\u003eERC sample recovery rate acceptance range: 50% e2 150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNegative control (NCS\/NTC) criteria\u003c\/td\u003e\r\n \u003ctd\u003eCt of NCS should be larger than mean Ct of the lowest standard; NTC Ct should be at least 2 cycles larger than mean Ct of lowest standard (or follow lab-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecommended instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System (SDS v1.4 settings example provided)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e-20 b0C, kit components can be stored at appropriate conditions for up to 24 months (check label expiry)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input\u003c\/td\u003e\r\n \u003ctd\u003e10 bL purified test sample or 10 bL standards\/DDB per reaction (per recommended workflow)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Quantitation down to femtogram level (fg), enabling detection of trace residual bovine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Uses a standard-curve based absolute quantitation method with serially diluted bovine DNA controls.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal process control:\u003c\/strong\u003e Includes an IPC mix to monitor PCR inhibition and validate sample results.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use qPCR MIX:\u003c\/strong\u003e Pre-formulated reaction buffer and primer\/probe mix minimize setup steps and reduce variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated for common real-time PCR platforms (example settings provided for ABI 7500 and SHENTEK-96S).\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual bovine DNA in biomaterials and biological raw materials\u003c\/li\u003e\r\n \u003cli\u003eQuality control of biologics, bioprocess intermediates and medical device materials\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and lot release testing for products where bovine-derived contaminants must be monitored\u003c\/li\u003e\r\n \u003cli\u003eResearch applications assessing bovine DNA contamination and extraction recovery\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine DNA Control (NNA032) 100 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eBovine Primer \u0026amp; Probe MIX (NNC042) 300 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003e-20 b0C, protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 bL d7 2 tubes\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 bL d7 1 tube\u003c\/span\u003e\u003cstrong\u003eRefer to manual\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL d7 3 tubes\u003c\/span\u003e\u003cstrong\u003e-20 b0C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO). Not for diagnostic or therapeutic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) before use; wear appropriate PPE (eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Use DNase-free, low-retention consumables; avoid contamination (UV-decontaminate surfaces and equipment, 75% ethanol disinfection).\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents at 2-8 b0C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Protect primer \u0026amp; probe mix from light; include appropriate controls (NTC, NCS, ERC) and run at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer becomes cloudy, warm at 37 b0C until clear; store unused DDB at 2-8 b0C.\u003c\/p\u003e\r\n \u003cp\u003e• UNG treatment step is optional and should be included only if UNG enzyme is added to the reaction mix.\u003c\/p\u003e\r\n \u003cp\u003e• Follow IPC and ERC acceptance criteria: IPC Ct within b11.0 of NCS; ERC recovery 50% to 150%.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101112.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the limit of detection (LOD) or limit of quantitation (LOQ) for the kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is designed for fg-level sensitivity. The provided standard curve ranges from 3000 pg\/bcl to 0.03 pg\/bcl; validated LOD\/LOQ should be established by the user in their laboratory during method validation.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What qPCR instrument settings are recommended?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eExample settings are provided for ABI 7500 (SDS v1.4): optional UNG 25 b0C 10:00; activation 95 b0C 10:00; 40 cycles of 95 b0C 00:15 and 63 b0C 00:40 (fluorescence read at 63 b0C). Adjust settings as needed for other platforms and software.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the supplied Bovine DNA Control serially in DNA Dilution Buffer to generate standards: ST0 = 30000 pg\/bcl (stock) then ST1 = 3000, ST2 = 300, ST3 = 30, ST4 = 3, ST5 = 0.3, ST6 = 0.03 pg\/bcl. Include at least five concentrations in the curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What controls should be included in each run?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eInclude No Template Control (NTC), Negative Control Sample (NCS), Extraction Reference Control (ERC), and serial standards (ST1-ST6). Run samples and controls in triplicate when possible.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175224959157,"sku":"1101112","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_Bovine_DNA_Quantitation_Kit.png?v=1742290060"},{"product_id":"residual-procine-dna-quantitation-kit-shentek®","title":"Residual Procine DNA Quantitation Kit SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: repeat(auto-fit, minmax(240px, 1fr));\n gap: 20px;\n}\n\n.shentek-grid--downloads {\n grid-template-columns: repeat(auto-fit, minmax(300px, 1fr));\n gap: 15px;\n}\n\n.shentek-list {\n padding-left: 20px;\n font-size: 14.5px;\n color: #4a5568;\n}\n\n.shentek-list li {\n margin-bottom: 8px;\n}\n\n.shentek-list li:last-child {\n margin-bottom: 0;\n}\n\n.shentek-box {\n background: #f8fafc;\n padding: 15px;\n border-radius: 6px;\n font-size: 13.5px;\n color: #4a5568;\n border: 1px solid #e2e8f0;\n}\n\n.shentek-box-row {\n display: flex;\n justify-content: space-between;\n padding: 6px 0;\n border-bottom: 1px dashed #cbd5e1;\n}\n\n.shentek-box-note {\n margin-top: 10px;\n font-size: 12px;\n color: #64748b;\n font-style: italic;\n}\n\n.shentek-box--warning {\n background: #fff5f5;\n border: 1px solid #feb2b2;\n color: #c53030;\n}\n\n.shentek-box--warning p {\n margin: 0;\n}\n\n.shentek-box--warning p + p {\n margin-top: 8px;\n}\n\n.shentek-card {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual Porcine DNA Quantitation Kit is designed to quantify residual porcine DNA in biomaterials using fluorescent quantitative PCR (qPCR). The kit provides a rapid, specific and reliable absolute quantitation assay with sensitivity down to the femtogram (fg) level. The product is supplied for 100 reactions (Product No. 1101113) and is intended for research use only. For sample extraction instructions, refer to the SHENTEK® Animal-derived Biomaterial Residual DNA Sample Preparation Kit (Product No. 1104193). The kit includes internal positive control (IPC) reagents and a porcine DNA standard control to build a standard curve for absolute quantitation.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eIntended use\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eQuantitation of residual porcine DNA in biomaterials (research use only)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetectable at femtogram (fg) level (kit optimized for absolute quantitation down to 0.03 pg\/µL standard point)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve range (prepared)\u003c\/td\u003e\r\n \u003ctd\u003eSerial standards prepared: ST0 30000 pg\/µL (stock), ST1 3000 pg\/µL, ST2 300 pg\/µL, ST3 30 pg\/µL, ST4 3 pg\/µL, ST5 0.3 pg\/µL, ST6 0.03 pg\/µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003eTotal reaction volume 30 µL (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR MIX composition (per reaction)\u003c\/td\u003e\r\n \u003ctd\u003eAT qPCR Reaction Buffer 15.9 µL; Porcine Primer \u0026amp; Probe MIX 2.8 µL; IPC MIX 1.3 µL; (optional UNG enzyme 0.1 U)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eOptional UNG 25 °C 10:00; Activation 95 °C 10:00; 40 cycles of 95 °C 00:15 (denaturation) and 60 °C 00:40 (annealing\/extension, fluorescence read)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eFluorophores \/ channels\u003c\/td\u003e\r\n \u003ctd\u003ePorcine-DNA: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings \/ thresholds\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct with Threshold = 0.02 for Porcine-DNA and 0.02 for IPC; Automatic baseline recommended\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eInternal control acceptance criteria\u003c\/td\u003e\r\n \u003ctd\u003eMean Ct of IPC for sample should be within ±1.0 Ct of the NCS (negative control) IPC Ct; large shift indicates inhibition\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eRecovery criteria (ERC)\u003c\/td\u003e\r\n \u003ctd\u003eExtraction Reference Control (ERC) recovery rate acceptable range 50%–150%\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eNTC \/ NCS criteria\u003c\/td\u003e\r\n \u003ctd\u003eNCS Ct should be larger than mean Ct of lowest standard concentration; NTC should be 2 cycles larger than mean Ct of lowest concentration (or laboratory-validated criteria)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReactions per kit\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 100 reactions\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStorage stability\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eKit components can be stored under recommended conditions for up to 24 months; key reagents stored at –20 °C\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eCompatible instruments\u003c\/td\u003e\r\n \u003ctd\u003eSHENTEK-96S Real-Time PCR System, ABI 7500 Real-Time PCR System; other real-time PCR systems supported with appropriate setup\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Optimized qPCR assay capable of femtogram-level detection for low-abundance residual porcine DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eAbsolute quantitation:\u003c\/strong\u003e Includes porcine DNA control and recommended serial dilutions to generate an absolute-quantitation standard curve.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal control (IPC):\u003c\/strong\u003e IPC MIX included to monitor extraction and PCR inhibition for each sample.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated AT qPCR Reaction Buffer and Porcine Primer \u0026amp; Probe MIX reduce setup time and variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eCarryover prevention option:\u003c\/strong\u003e Supports optional UNG treatment step when UNG enzyme is included to prevent carryover contamination.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eValidated workflow:\u003c\/strong\u003e Detailed guidance for serial dilution, plate layout, thermal cycling and result analysis for reproducible assays.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual porcine DNA in biomaterials (e.g., tissue-derived products, implants, scaffolds)\u003c\/li\u003e\r\n \u003cli\u003eQuality control and release testing for animal-derived material processing\u003c\/li\u003e\r\n \u003cli\u003eMethod validation and extraction recovery assessment using ERC samples\u003c\/li\u003e\r\n \u003cli\u003eResearch studies requiring sensitive detection of porcine DNA contamination\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine DNA Control (NNA031) 100 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eAT qPCR Reaction Buffer (NNB012) 850 µL × 2\u003c\/span\u003e\u003cstrong\u003e–20 °C; protect from light\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003ePorcine Primer \u0026amp; Probe MIX (NNC043) 300 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC066) 150 µL × 1\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001) 1.5 mL × 3\u003c\/span\u003e\u003cstrong\u003e–20 °C\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDocumentation: User Guide (this manual)\u003c\/span\u003e\u003cstrong\u003eambient\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 100 Reactions | Method: qPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO); not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions. Wear appropriate PPE (eyewear, mask, clothing, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Decontaminate work surfaces, pipettes and tubes by UV irradiation (30 minutes) and 75% ethanol prior to setup to minimize contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw kit reagents at 2–8 °C or on ice, vortex and briefly centrifuge before use.\u003c\/p\u003e\r\n \u003cp\u003e• Store reagents at –20 °C; check expiration dates on labels. Protect light-sensitive reagents.\u003c\/p\u003e\r\n \u003cp\u003e• Include at least five standard concentrations when preparing the standard curve; recommended serial dilution series provided (ST1–ST6).\u003c\/p\u003e\r\n \u003cp\u003e• Prepare NTC, NCS and ERC controls as instructed. Test samples and controls in triplicate for reliable results.\u003c\/p\u003e\r\n \u003cp\u003e• If UNG is used, include 25 °C 10:00 UNG step in the thermal program; UNG is optional but recommended to prevent carryover.\u003c\/p\u003e\r\n \u003cp\u003e• Monitor IPC Ct values to assess inhibition; acceptable IPC Ct shift is ±1.0 relative to NCS.\u003c\/p\u003e\r\n \u003cp\u003e• Acceptable ERC recovery rate is 50%–150%; if outside this range investigate extraction and inhibition.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1101113.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the intended use of this kit?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit is intended for research use only to quantitatively measure residual porcine DNA in biomaterials using qPCR.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: What is the recommended reaction setup and volume?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eTotal reaction volume is 30 µL (20 µL qPCR MIX + 10 µL sample or standard). qPCR MIX per reaction: AT qPCR Reaction Buffer 15.9 µL, Porcine Primer \u0026amp; Probe MIX 2.8 µL, IPC MIX 1.3 µL. Optional UNG 0.1 U per reaction.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How do I prepare the standard curve?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eDilute the provided Porcine DNA Control with DNA Dilution Buffer to produce a serial dilution series: ST0 30000 pg\/µL then ST1 3000, ST2 300, ST3 30, ST4 3, ST5 0.3, ST6 0.03 pg\/µL. Include at least five concentrations for the standard curve.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: What criteria indicate assay inhibition or failure?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eIf the sample IPC mean Ct is \u0026gt; ±1.0 Ct from the NCS IPC Ct, the sample may be inhibitory. ERC recovery outside 50%–150% or abnormal NTC\/NCS Ct values also indicate possible issues; troubleshoot extraction, sample dilution, or inhibition.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"100 Reactions","offer_id":45175394730165,"sku":"1101113","price":1545.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/dna-Residual_Procine_DNA_Quantitation_Kit.png?v=1742290235"},{"product_id":"residual-cho-dna-size-analysis-kit-2g-shentek®","title":"Residual CHO DNA Size Analysis Kit (2G) SHENTEK®","description":"\u003cstyle type=\"text\/css\"\u003e\n.shentek-wrapper {\n font-family: 'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n color: #2c3e50;\n line-height: 1.7;\n margin: 0 auto;\n background: #fff;\n padding: 10px;\n}\n\n.shentek-header-bar {\n background-color: #0056b3;\n border-radius: 8px;\n padding: 20px;\n display: flex;\n flex-wrap: wrap;\n justify-content: space-around;\n align-items: center;\n color: #ffffff;\n margin-bottom: 30px;\n box-shadow: 0 4px 6px rgba(0, 0, 0, 0.1);\n}\n\n.shentek-header-item {\n text-align: center;\n padding: 10px;\n min-width: 200px;\n}\n\n.shentek-header-item--bordered {\n \n border-right: 1px solid rgba(255, 255, 255, 0.2);\n}\n\n.shentek-header-label {\n font-size: 14px;\n opacity: 0.9;\n margin-bottom: 5px;\n}\n\n.shentek-header-value {\n font-size: 18px;\n font-weight: bold;\n}\n\n.shentek-section {\n margin-bottom: 45px;\n}\n\n.shentek-section--divider {\n border-bottom: 2px solid #f0f4f8;\n padding-bottom: 25px;\n margin-bottom: 40px;\n}\n\n.shentek-section--card {\n background: #fcfdfe;\n border: 1px solid #d1d5db;\n border-radius: 12px;\n padding: 30px;\n}\n\n.shentek-title {\n color: #0056b3;\n font-size: 24px;\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-title-bar {\n background: #0056b3;\n width: 4px;\n height: 24px;\n margin-right: 12px;\n display: inline-block;\n}\n\n.shentek-subtitle {\n color: #2d3748;\n font-size: 20px;\n margin-bottom: 20px;\n}\n\n.shentek-subtitle--bar {\n \n padding-left: 15px;\n margin-bottom: 25px;\n}\n\n.shentek-subtitle--icon {\n margin-top: 0;\n display: flex;\n align-items: center;\n}\n\n.shentek-subtitle-icon {\n margin-right: 12px;\n}\n\n.shentek-heading {\n color: #2d3748;\n font-size: 18px;\n \n padding-left: 12px;\n margin-bottom: 15px;\n}\n\n.shentek-text {\n font-size: 16px;\n color: #4a5568;\n margin-top: 15px;\n}\n\n.shentek-table-wrapper {\n overflow-x: auto;\n}\n\n.shentek-table {\n width: 100%;\n border-collapse: collapse;\n border: 1px solid #e2e8f0;\n font-size: 15px;\n}\n\n.shentek-table thead tr {\n background-color: #0056b3;\n color: #fff;\n text-align: left;\n}\n\n.shentek-table th {\n padding: 15px;\n}\n\n.shentek-table td {\n padding: 12px 15px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-table td:first-child {\n font-weight: 600;\n background: #f8fafc;\n}\n\n.shentek-table .shentek-text-danger {\n color: #c53030;\n font-weight: 600;\n}\n\n.shentek-grid {\n display: grid;\n gap: 30px;\n}\n\n.shentek-grid--2col {\n grid-template-columns: repeat(auto-fit, minmax(450px, 1fr));\n}\n\n.shentek-grid--4col {\n grid-template-columns: 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8px;\n padding: 20px;\n text-align: center;\n background: #fff;\n}\n\n.shentek-card-title {\n font-size: 14px;\n margin: 0 0 15px 0;\n height: 40px;\n color: #2d3748;\n}\n\n.shentek-btn {\n display: block;\n padding: 8px;\n background: #0056b3;\n color: #fff;\n text-decoration: none;\n border-radius: 4px;\n font-size: 13px;\n font-weight: 600;\n}\n\n.shentek-download-link {\n display: flex;\n align-items: center;\n background: #fff;\n padding: 12px 18px;\n border-radius: 6px;\n border: 1px solid #cbd5e1;\n text-decoration: none;\n color: #334155;\n font-size: 14px;\n font-weight: 500;\n}\n\n.shentek-download-link .shentek-pdf-icon {\n color: #ef4444;\n font-weight: bold;\n margin-right: 10px;\n}\n\n.shentek-download-link .shentek-arrow {\n margin-left: auto;\n color: #94a3b8;\n}\n\n.shentek-faq {\n border: 1px solid #e2e8f0;\n border-radius: 8px;\n overflow: hidden;\n}\n\n.shentek-faq-item {\n padding: 15px 20px;\n border-bottom: 1px solid #e2e8f0;\n}\n\n.shentek-faq-item:last-child {\n border-bottom: none;\n}\n\n.shentek-faq-item--alt {\n background-color: #f8fafc;\n}\n\n.shentek-faq-question {\n font-weight: bold;\n color: #0056b3;\n margin-bottom: 5px;\n}\n\n.shentek-faq-answer {\n font-size: 14px;\n color: #4a5568;\n}\n\n.shentek-notice {\n background-color: #fffbeb;\n border: 1px solid #fde68a;\n border-radius: 8px;\n padding: 20px;\n margin-top: 30px;\n margin-bottom: 30px;\n}\n\n.shentek-notice-header {\n display: flex;\n align-items: center;\n gap: 8px;\n margin-bottom: 12px;\n}\n\n.shentek-notice-icon {\n width: 20px;\n height: 20px;\n color: #d97706;\n flex-shrink: 0;\n}\n\n.shentek-notice-title {\n font-weight: bold;\n color: #78350f;\n margin: 0;\n font-size: 16px;\n}\n\n.shentek-notice-body {\n margin-left: 28px;\n}\n\n.shentek-notice-item {\n display: flex;\n align-items: flex-start;\n gap: 8px;\n margin-bottom: 10px;\n}\n\n.shentek-notice-item:last-child {\n margin-bottom: 0;\n}\n\n.shentek-notice-check {\n color: #d97706;\n font-weight: bold;\n flex-shrink: 0;\n}\n\n.shentek-notice-text {\n margin: 0;\n font-size: 14px;\n color: #374151;\n line-height: 1.5;\n}\n\u003c\/style\u003e\r\n\r\n\u003cdiv class=\"shentek-wrapper\"\u003e\r\n \u003cdiv class=\"shentek-header-bar\"\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Detection Method\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eqPCR\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item shentek-header-item--bordered\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Assay Time\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003eRefer to manual\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-item\"\u003e\r\n \u003cdiv class=\"shentek-header-label\"\u003e Storage Temperature\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-header-value\"\u003e–20 °C (stable for 24 months)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\r\n \u003csection class=\"shentek-section shentek-section--divider\"\u003e\r\n \u003ch2 class=\"shentek-title\"\u003e\r\n \r\n Product Description\r\n \u003c\/h2\u003e\r\n \u003cp class=\"shentek-text\"\u003eSHENTEK® Residual CHO DNA Size Analysis Kit (2G) is a fluorescent quantitative PCR (FAM) based kit for rapid and specific quantitation of residual Chinese Hamster Ovary (CHO) host cell DNA fragments across multiple size ranges in biopharmaceutical samples (from in-process to final products). The assay amplifies four discrete fragment sizes (95 bp, 110 bp, 215 bp and 523 bp) to produce an accurate size distribution profile. The kit includes primer\/probe mixes for each fragment, an internal positive control (IPC), qPCR reaction buffer, CHO DNA control for standard curves and DNA dilution buffer (DDB). The assay sensitivity reaches femtogram (fg) level. Refer to SHENTEK® Residual Host Cell DNA Sample Preparation Kit (Product No. 1104191) for extraction procedures. Product No.: 1103170-2. Reagents sufficient for 4 × 100 reactions.\u003c\/p\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle\"\u003eTechnical Specifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-table-wrapper\"\u003e\r\n \u003ctable class=\"shentek-table\"\u003e\r\n \u003cthead\u003e\r\n \u003ctr\u003e\r\n \u003cth\u003eParameter\u003c\/th\u003e\r\n \u003cth\u003eDetails\u003c\/th\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/thead\u003e\r\n \u003ctbody\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eTarget fragment sizes\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003e95 bp, 110 bp, 215 bp, 523 bp\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eDetection chemistry \/ dyes\u003c\/td\u003e\r\n \u003ctd\u003eTarget probes: FAM reporter; IPC: VIC reporter; ROX as passive reference\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSensitivity (LOD)\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eDetection limit reaches femtogram (fg) level\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eStandard curve concentrations (examples)\u003c\/td\u003e\r\n \u003ctd\u003eST1 = 300 pg\/µL; ST2 = 30 pg\/µL; ST3 = 3 pg\/µL; ST4 = 0.3 pg\/µL; ST5 = 0.03 pg\/µL (ST0 = 3000 pg\/µL stock)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSuggested linear range\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eApproximately 0.03 – 300 pg\/µL (based on provided standard curve points)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eReaction volume\u003c\/td\u003e\r\n \u003ctd\u003e30 µL total reaction volume (20 µL qPCR MIX + 10 µL sample\/standard)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eqPCR mix per reaction\u003c\/td\u003e\r\n \u003ctd\u003eqPCR Reaction Buffer 15.9 µL + CHO Primer\u0026amp;Probe MIX (per fragment) 2.8 µL + IPC MIX 1.3 µL = 20 µL\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eSample input per reaction\u003c\/td\u003e\r\n \u003ctd\u003e10 µL (standard\/ST1–ST5, DDB for NCS, or purified sample)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eThermal cycling conditions\u003c\/td\u003e\r\n \u003ctd\u003eActivation: 95 °C 10:00 (1 cycle); Denaturation: 95 °C 00:15; Annealing: 60 °C 00:30; Extension\/Read: 72 °C 01:30; 40 cycles. Fluorescence read during extension step.\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAnalysis settings (recommended)\u003c\/td\u003e\r\n \u003ctd\u003eManual Ct selection; Threshold = 0.02; Automatic baseline; Standard curve (Absolute Quantitation)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003ctr\u003e\r\n \u003ctd\u003eAssay throughput\u003c\/td\u003e\r\n \u003ctd class=\"shentek-text-danger\"\u003eReagents provided for 4 × 100 reactions (kit components support ~400 reactions distributed across four fragment assays)\u003c\/td\u003e\r\n \u003c\/tr\u003e\r\n \u003c\/tbody\u003e\r\n \u003c\/table\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--2col\"\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eFeatures\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eMulti-size profiling:\u003c\/strong\u003e Simultaneous measurement strategy (four separate assays) for size distribution of CHO residual DNA (95, 110, 215, 523 bp) to characterize fragmentation.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eHigh sensitivity:\u003c\/strong\u003e Detection limit at femtogram (fg) level enables quantitation of very low-level residual host cell DNA.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eReady-to-use mixes:\u003c\/strong\u003e Pre-formulated CHO Primer \u0026amp; Probe MIXes and qPCR Reaction Buffer simplify setup and reduce pipetting variability.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInternal positive control (IPC):\u003c\/strong\u003e IPC mix included (VIC-labeled) to monitor inhibition and reaction performance.\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eInstrument compatibility:\u003c\/strong\u003e Validated on common real-time PCR systems (e.g., SHENTEK-96S, ABI 7500, Bio-Rad CFX96, Linegene 9600 plus).\u003c\/li\u003e\r\n \u003cli\u003e\n\u003cstrong\u003eComplete workflow support:\u003c\/strong\u003e Includes DNA control and DNA dilution buffer for standard curves plus protocol for plate layouts and analysis.\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eApplications\u003c\/h3\u003e\r\n \u003cul class=\"shentek-list\"\u003e\r\n \u003cli\u003eQuantitation of residual CHO host cell DNA in biopharmaceutical in-process samples\u003c\/li\u003e\r\n \u003cli\u003eQuantitation and size-distribution analysis of residual CHO DNA in final drug substance\/product\u003c\/li\u003e\r\n \u003cli\u003eMethod development and validation for host cell DNA monitoring\u003c\/li\u003e\r\n \u003cli\u003eRoutine QC testing of downstream purification and final product release (research and development settings)\u003c\/li\u003e\r\n \u003c\/ul\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eKit Contents\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box\"\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eqPCR Reaction Buffer (NNB002)\u003c\/span\u003e\u003cstrong\u003e–20 °C, protect from light (850 μL × 8 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-95 (NNC087)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-110 (NNC088)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-215 (NNC089)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO Primer\u0026amp;Probe MIX-523 (NNC090)\u003c\/span\u003e\u003cstrong\u003e–20 °C (300 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eIPC MIX (NNC069)\u003c\/span\u003e\u003cstrong\u003e–20 °C (550 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eCHO DNA Control (NNA001)\u003c\/span\u003e\u003cstrong\u003e–20 °C (50 μL × 1 tube)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-row\"\u003e\r\n \u003cspan\u003eDNA Dilution Buffer (DDB) (NND001)\u003c\/span\u003e\u003cstrong\u003e2–8 °C recommended after thaw; (1.5 mL × 3 tubes)\u003c\/strong\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-box-note\"\u003e* Total: 4 × 100 Reactions | Method: qPCR (Fluorescent quantitative PCR, FAM)\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv\u003e\r\n \u003ch3 class=\"shentek-heading\"\u003eAttention\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-box shentek-box--warning\"\u003e\r\n \u003cp\u003e• For Research Use Only (RUO) — not for diagnostic use.\u003c\/p\u003e\r\n \u003cp\u003e• Read Material Safety Data Sheets (MSDS) and follow handling instructions.\u003c\/p\u003e\r\n \u003cp\u003e• Wear appropriate PPE (protective eyewear, mask, lab coat, gloves).\u003c\/p\u003e\r\n \u003cp\u003e• Work in DNase-free environment; use DNase-free consumables and low-retention tips to avoid contamination.\u003c\/p\u003e\r\n \u003cp\u003e• Irradiate workspace, pipettes and tubes with UV for 30 minutes and disinfect with 75% alcohol before use.\u003c\/p\u003e\r\n \u003cp\u003e• Thaw reagents completely at 2–8 °C or on ice; protect light-sensitive reagents from light.\u003c\/p\u003e\r\n \u003cp\u003e• Store kit components at –20 °C and check expiration dates. Kit stable up to 24 months under recommended storage.\u003c\/p\u003e\r\n \u003cp\u003e• Prepare separate standard curves for each fragment length (95, 110, 215, 523 bp). Include at least five standard concentrations.\u003c\/p\u003e\r\n \u003cp\u003e• If DNA Dilution Buffer (DDB) is cloudy or contains precipitates, heat at 37 °C until clear before use.\u003c\/p\u003e\r\n \u003cp\u003e• Use NTC (no template control), NCS (negative control sample) and ERC (extraction reference control) as directed to monitor contamination and extraction recovery.\u003c\/p\u003e\r\n \u003cp\u003e• Mix qPCR reactions thoroughly, seal plate, spin briefly and avoid bubble formation prior to run.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \r\n \u003csection class=\"shentek-section shentek-section--lg\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eQuality Management \u0026amp; Certifications\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--4col\"\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality System\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload QMS\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQMS (ISO, GMP)\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Certificate\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Advantages\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eView Report\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-card\"\u003e\r\n \u003ch4 class=\"shentek-card-title\"\u003eQuality Control Process\u003c\/h4\u003e\r\n \u003ca class=\"shentek-btn\" href=\"#\"\u003eDownload Process\u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\u003csection class=\"shentek-section shentek-section--card\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--icon\"\u003e\r\n \u003cspan class=\"shentek-subtitle-icon\"\u003e📂\u003c\/span\u003e Technical Resources \u0026amp; Downloads\r\n \u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-grid shentek-grid--downloads\"\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/coa\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e Product Manual \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003ca class=\"shentek-download-link\" href=\"https:\/\/files.biofargo.com\/shentek\/msds\/1103170-2.pdf\" target=\"_blank\"\u003e\r\n \u003cspan class=\"shentek-pdf-icon\"\u003ePDF\u003c\/span\u003e MSDS \/ SDS \u003cspan class=\"shentek-arrow\"\u003e↓\u003c\/span\u003e\r\n \u003c\/a\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003csection class=\"shentek-section\"\u003e\r\n \u003ch3 class=\"shentek-subtitle shentek-subtitle--bar\"\u003eFrequently Asked Questions (FAQ)\u003c\/h3\u003e\r\n \u003cdiv class=\"shentek-faq\"\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ1: What is the assay sensitivity (limit of detection)?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit sensitivity reaches the femtogram (fg) level. Standard curve points provided include concentrations down to 0.03 pg\/µL (30 fg\/µL) and a stock at 3000 pg\/µL; actual LOD may depend on instrument and sample matrix.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ2: Which real-time PCR instruments are compatible?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit has been used with SHENTEK-96S, ABI 7500, Bio-Rad CFX96 and Linegene 9600 plus. Other instruments supporting FAM\/VIC detection and ROX (if needed) may be used — consult instrument documentation for detector\/dye settings.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item shentek-faq-item--alt\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ3: How should the kit be stored and what is its shelf life?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eStore kit components at –20 °C, protect light-sensitive reagents from light. Under recommended storage the kit is stable for up to 24 months; always verify the expiration date on labels.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-item\"\u003e\r\n \u003cdiv class=\"shentek-faq-question\"\u003eQ4: How many reactions does the kit support and how are reactions prepared?\u003c\/div\u003e\r\n \u003cdiv class=\"shentek-faq-answer\"\u003eThe kit provides reagents for 4 × 100 reactions. Typical reaction setup is 20 µL qPCR MIX + 10 µL sample = 30 µL total. Prepare separate qPCR mixes for each fragment assay (95\/110\/215\/523) as described in the protocol.\u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/section\u003e\r\n\r\n \u003cdiv class=\"shentek-notice\"\u003e\r\n \u003cdiv class=\"shentek-notice-header\"\u003e\r\n \u003csvg class=\"shentek-notice-icon\" viewbox=\"0 0 20 20\" fill=\"currentColor\"\u003e\r\n \u003cpath fill-rule=\"evenodd\" d=\"M8.257 3.099c.765-1.36 2.722-1.36 3.486 0l5.58 9.92c.75 1.334-.213 2.98-1.742 2.98H4.42c-1.53 0-2.493-1.646-1.743-2.98l5.58-9.92zM11 13a1 1 0 11-2 0 1 1 0 012 0zm-1-8a1 1 0 00-1 1v3a1 1 0 002 0V6a1 1 0 00-1-1z\" clip-rule=\"evenodd\"\u003e\u003c\/path\u003e\r\n \u003c\/svg\u003e\r\n \u003ch3 class=\"shentek-notice-title\"\u003eResearch Use Only\u003c\/h3\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-body\"\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003e\u003cstrong\u003eResearch Use Only (RUO)\u003c\/strong\u003e – This product is intended\r\n for laboratory research purposes only and not for clinical or regulatory diagnostic use.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003cdiv class=\"shentek-notice-item\"\u003e\r\n \u003cspan class=\"shentek-notice-check\"\u003e✔\u003c\/span\u003e\r\n \u003cp class=\"shentek-notice-text\"\u003eNot intended for use in USDA or FDA regulated diagnostic testing or\r\n official compliance testing.\u003c\/p\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n \u003c\/div\u003e\r\n\u003c\/div\u003e","brand":"SHENTEK","offers":[{"title":"4 × 100 Reactions","offer_id":45175424188597,"sku":"1103170-2","price":3708.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Residual_CHO_DNA_Size_Analysis_Kit_2G.png?v=1742290344"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/dsDNA_8de9d8ed-09c7-4052-8186-538636a7583f.png?v=1757006050","url":"https:\/\/biofargo.com\/collections\/dna-rna-extraction-analysis-1.oembed?page=12","provider":"Biofargo","version":"1.0","type":"link"}