{"title":"Cell Culture Reagents","description":"","products":[{"product_id":"fgf-9-murine-mouse","title":"FGF-9, murine (mouse)","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eFGF-9, murine (mouse): Fibroblast growth factor 9 (FGF-9) encoded by the FGF-9 gene is a member of the fibroblast growth factor (FGF) family. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. In addition, this protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells and it is a heparin-binding protein. Furthermore, FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Recombinant mouse FGF-9 is synthesized as a 208 a.a. precursor that contains a 3 a.a. signal sequence. Specifically, The mouse FGF-9 shares 99 % a. a. sequence identity with rat FGF-9\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUnitProt ID: P54130\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNumber of Containers\u003c\/strong\u003e:1\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eRefrigeration Requirements\u003c\/strong\u003e:Freezer\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eShipping Conditions\u003c\/strong\u003e:ICE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Code\u003c\/strong\u003e:41116127\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eUNSPSC Category\u003c\/strong\u003e:Other Cytokines and Chemokines\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDG\u003c\/strong\u003e:No\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e:(-15 to -20℃)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eCSterile\u003c\/strong\u003e:Yes\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"10ug","offer_id":44905328804021,"sku":"SD7130-10μg","price":186.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/10_a4f71059-96eb-41f2-a3f6-34976f07278d.jpg?v=1753176990"},{"product_id":"xiyuan™-r-transfection-reagent","title":"Transfection Reagent","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTransfection Reagent is a next-generation cationic lipid-based delivery system optimized for efficient transfection of plasmid DNA, messenger RNA (mRNA), and small interfering RNA (siRNA) into a broad range of eukaryotic cells. Its proprietary lipid nanoparticle formulation ensures strong gene expression results while minimizing cytotoxicity—making it ideal for both routine and advanced applications, including CRISPR and RNA-based therapeutics research.\u003c\/p\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/2_6fe3ef81-c300-4db4-9186-6d2293bb4d91_480x480.jpg?v=1750489343\" alt=\"\" style=\"float: none;\"\u003e\u003c\/div\u003e\n\u003cdiv style=\"text-align: start;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_47349429-9f38-4cef-9c86-44125e81e2a0_480x480.jpg?v=1750489352\" style=\"margin-bottom: 16px; float: none;\"\u003e\u003c\/div\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh-Efficiency Transfection of mRNA: \u003c\/strong\u003eComparable in performance to Thermo Fisher’s Lipofectamine™ MessengerMAX™, Biofargo achieves excellent mRNA delivery with minimal toxicity—ideal for mRNA vaccines, gene editing, and protein expression studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eVersatile Nucleic Acid Compatibility: \u003c\/strong\u003eEnables efficient transfection of plasmid DNA, mRNA, siRNA, and other nucleic acid formats using a unified protocol.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eSuperior Cell Viability: \u003c\/strong\u003eReduces cellular stress and preserves morphology, ensuring reliable downstream analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCompatible with Serum and Antibiotics: \u003c\/strong\u003eNo need to remove serum or antibiotics, simplifying workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eProven in Difficult-to-Transfect Cell Lines: \u003c\/strong\u003eEffective with primary cells, stem cells, suspension cells, and commonly used lines like HEK293, HeLa, and CHO.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003emRNA transfection and expression\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003esiRNA-mediated gene knockdown\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGene editing (CRISPR\/Cas9)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eProtein production\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFunctional genomics and cell engineering\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003esiRNA Transfection Assay\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. Primary rat cardiomyocytes: siRNA knockdown efficiency of 82%.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/sy1.jpg?v=1750490245\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. BMDM cells: siRNA knockdown efficiency of 60% with no detectable cytotoxicity.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/sy2.jpg?v=1750490252\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. RAW264.7 cells: siRNA knockdown efficiency of 70%.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/sy3.jpg?v=1750490252\" alt=\"\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4. U87MG cells: siRNA knockdown efficiency of 85%, accompanied by significant inhibition of cell proliferation.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/sy4.jpg?v=1750490252\"\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003eReference for Cell Transfection Efficiency\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 87.354%; border-collapse: collapse; height: 694px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCell Name\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eKnockdown Efficiency\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHeLa\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eU-2 OS\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHEK293\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHCT-116\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eBHK-21\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCOS-7\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHT-1080\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eNCI-H441\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eU-87 MG\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e85%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eGlial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 10px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 10px;\"\u003ePrimary Rat Cortical Cells\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 10px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003ePrimary Rat Hippocampal\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHepG2\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eA549\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eK-562\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMCF7\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eJurkat\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTHP-1\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eL-929\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e3T3\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHuh7\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eNK-92\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaji\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRat Cardiomyocytes\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSaos-2\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eU-937\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSW480\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eL6\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003ePC-3\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e4T1\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e80%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw264.7\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHUVEC\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eHuman T Cells\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSH-SY5Y\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e70%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMouse Embryonic StemCells\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003ePBMC\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eBMDM\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMesenchymal Stem Cell (hMSC)\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003e60%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eQ: Can I deliver single-stranded oligonucleotides with Transfection Reagent?\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eA: \u003c\/strong\u003eIf delivering a single-stranded oligonucleotide alone, it's recommended to use Lipofectamine RNAiMAX Transfection Reagent. However, Transfection Reagent can be used for genome editing when co-delivering Cas9 mRNA, sgRNA, and a donor template (ssDNA or dsDNA).\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: How do I use Transfection Reagent to deliver both Cas9 mRNA and IVT gRNA together?\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eA: \u003c\/strong\u003eTransfection Reagent supports co-delivery of Cas9 mRNA with single or multiple gRNAs, providing high transfection efficiency. It is compatible with cleavage detection or selection kits for downstream mutation analysis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: Which cell types can be used with Transfection Reagent?\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eA: \u003c\/strong\u003eTransfection Reagent shows low cytotoxicity and high efficiency in various cell types, including primary and stem cells, as well as those that are typically difficult to transfect.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: When should I use Transfection Reagent over other reagents?\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eA: \u003c\/strong\u003eUse Transfection Reagent for CRISPR applications requiring the co-delivery of mRNA and gRNA, along with short donor DNA templates. Its flexibility makes it ideal for HDR-based genome editing.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eQ: What is Transfection Reagent?\u003c\/strong\u003e\u003cbr\u003e\u003cstrong\u003eA: \u003c\/strong\u003eTransfection Reagent is an animal-origin-free reagent formulated for delivery of mRNA, small RNA (e.g., gRNA, siRNA, miRNA), and short donor DNA (0.5–1 kb), making it highly suitable for CRISPR genome editing workflows.\u003c\/p\u003e","brand":"Xiyuan","offers":[{"title":"1mL","offer_id":51243999854773,"sku":"TR001","price":567.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/e79eb5b22e58849ca01a8a387049bd7.jpg?v=1750493424"},{"product_id":"amms®-organoid-specific-matrigel","title":"AMMS® Organoid-specific Matrigel","description":"\u003cstyle\u003e\n  .biofargo-wrap{\n    font-family:'Segoe UI', Roboto, Helvetica, Arial, sans-serif;\n    color:#333;\n    line-height:1.65;\n    max-width:1000px;\n    margin:0 auto;\n    background:#fff;\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:28px;\n  }\n  .biofargo-h2{\n    color:#0056b3;\n    border-bottom:2px solid #0056b3;\n    padding-bottom:8px;\n    margin:0 0 14px 0;\n    font-size:22px;\n  }\n  .biofargo-section{margin-top:28px;}\n  .biofargo-card{\n    background:#fcfcfc;\n    border:1px solid #f0f0f0;\n    border-radius:10px;\n    padding:16px;\n  }\n  .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n  }\n  table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:860px;\n    background:#fff;\n  }\n  table.biofargo-table th,\n  table.biofargo-table td{\n    border:1px solid #ddd;\n    padding:12px;\n    vertical-align:middle;\n  }\n  table.biofargo-table thead th{\n    background:#0056b3;\n    color:#fff;\n    font-weight:700;\n    text-align:left;\n  }\n  table.biofargo-table thead th.center,\n  table.biofargo-table tbody td.center{\n    text-align:center;\n  }\n  table.biofargo-table tbody td:first-child{\n    font-weight:700;\n    background:#f8f9fa;\n  }\n  .biofargo-checklist{\n    list-style:none;\n    padding:0;\n    margin:0;\n  }\n  .biofargo-checklist li{\n    position:relative;\n    padding-left:28px;\n    margin-bottom:14px;\n  }\n  .biofargo-check{\n    position:absolute;\n    left:0;\n    top:0;\n    color:#0056b3;\n    font-weight:800;\n  }\n  .biofargo-grid{\n    display:grid;\n    grid-template-columns:repeat(2, minmax(0, 1fr));\n    gap:16px;\n  }\n  .biofargo-app-card{\n    border:1px solid #e1e8ed;\n    border-radius:12px;\n    padding:14px;\n    background:#fff;\n  }\n  .biofargo-app-card h4{\n    margin:0 0 6px 0;\n    font-size:16px;\n    color:#333;\n  }\n  .biofargo-app-card p{\n    margin:0;\n    font-size:14px;\n  }\n  .biofargo-docs{\n    display:flex;\n    flex-wrap:wrap;\n    gap:12px;\n    margin-top:12px;\n  }\n  .biofargo-btn{\n    display:inline-block;\n    padding:10px 18px;\n    border-radius:8px;\n    font-weight:800;\n    font-size:14px;\n    text-decoration:none;\n  }\n  .biofargo-btn.primary{\n    background:#0056b3;\n    color:#fff;\n    border:2px solid #0056b3;\n  }\n  .biofargo-btn.outline{\n    background:#fff;\n    color:#0056b3;\n    border:2px solid #0056b3;\n  }\n  @media (max-width:640px){\n    .biofargo-wrap{padding:18px;}\n    .biofargo-grid{grid-template-columns:1fr;}\n  }\n\u003c\/style\u003e\n\n\u003cdiv class=\"biofargo-wrap\"\u003e\n\n  \u003c!-- Description --\u003e\n  \u003cdiv class=\"biofargo-section\" style=\"margin-top:0;\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eDescription\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-card\"\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eAMMS® organoid-specific matrix gel is made from basement membrane components extracted from mouse tumor tissues, and its main components include laminin, type IV collagen, heparin sulfate proteoglycans, etc.\u003c\/p\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eThese components can provide the support and signals required for cell adhesion, differentiation and proliferation, and can also simulate the characteristics of basement membranes in physiological environments, thereby improving the success rate and effect of cell culture.\u003c\/p\u003e\n      \u003cp style=\"margin:0 0 10px 0;\"\u003eIn addition to basement membrane components, AMMS® organoid matrix is also rich in a variety of growth factors. These growth factors can promote cell differentiation, proliferation and migration, thereby further simulating cell signaling pathways and interactions in the physiological environment.\u003c\/p\u003e\n      \u003cp style=\"margin:0;\"\u003eAMMS® organoid matrix has a wide range of application prospects, especially in tissue engineering, cell culture and research, and can be used for organoid culture, stem cell differentiation, angiogenesis, migration or invasion, and in vivo tumorigenesis.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Specifications --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eSpecifications\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-table-wrap\"\u003e\n      \u003ctable class=\"biofargo-table\"\u003e\n        \u003cthead\u003e\n          \u003ctr\u003e\n            \u003cth style=\"width:25%;\"\u003eParameter\u003c\/th\u003e\n            \u003cth style=\"width:37.5%;\"\u003eSpecification\u003c\/th\u003e\n            \u003cth class=\"center\" style=\"width:37.5%;\"\u003eResults\u003c\/th\u003e\n          \u003c\/tr\u003e\n        \u003c\/thead\u003e\n        \u003ctbody\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eAppearance\u003c\/td\u003e\n            \u003ctd\u003eYellow jelly-like\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eProtein Concentration\u003c\/td\u003e\n            \u003ctd\u003e\u0026gt;8 mg\/mL\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eGel Time\u003c\/td\u003e\n            \u003ctd\u003e\u0026lt;30 min\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eLactic Dehydrogenase Virus (LDEV)\u003c\/td\u003e\n            \u003ctd\u003ePCR-negative\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eFungi, Bacteria, Mycoplasma\u003c\/td\u003e\n            \u003ctd\u003eShould be negative\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eGel Stability (37°C, 14 Days)\u003c\/td\u003e\n            \u003ctd\u003eStabilize\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eStem Cell Culture\u003c\/td\u003e\n            \u003ctd\u003ePotential growth\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eNerve Axon Culture\u003c\/td\u003e\n            \u003ctd\u003ePotential growth\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003eEndotoxin\u003c\/td\u003e\n            \u003ctd\u003e\u0026lt;1.5 EU\/mL\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n          \u003ctr\u003e\n            \u003ctd\u003ePackage\u003c\/td\u003e\n            \u003ctd\u003eNo damage, no leakage\u003c\/td\u003e\n            \u003ctd class=\"center\"\u003eComply with regulations\u003c\/td\u003e\n          \u003c\/tr\u003e\n        \u003c\/tbody\u003e\n      \u003c\/table\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Features --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eFeatures\u003c\/h2\u003e\n    \u003cul class=\"biofargo-checklist\"\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eBasement Membrane–Derived Matrix Composition\u003c\/strong\u003e\n        \u003cp\u003eComposed primarily of laminin, type IV collagen, and heparin sulfate proteoglycans, providing structural and biochemical cues similar to native basement membranes.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eGrowth Factor–Enriched Microenvironment\u003c\/strong\u003e\n        \u003cp\u003eContains multiple endogenous growth factors that support cell differentiation, proliferation, migration, and intercellular signaling in three-dimensional culture systems.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eRapid Gelation for 3D Culture Systems\u003c\/strong\u003e\n        \u003cp\u003eForms a stable gel within 30 minutes under appropriate conditions, enabling reliable scaffold formation for organoid embedding and culture.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eHigh Protein Concentration Matrix\u003c\/strong\u003e\n        \u003cp\u003eProtein concentration greater than 8 mg\/mL provides sufficient extracellular matrix density for structural stability and cell support.\u003c\/p\u003e\n      \u003c\/li\u003e\n      \u003cli\u003e\n        \u003cspan class=\"biofargo-check\"\u003e✔\u003c\/span\u003e\n        \u003cstrong\u003eQuality-Controlled Biological Safety\u003c\/strong\u003e\n        \u003cp\u003eTested negative for LDEV, fungi, bacteria, and mycoplasma, with endotoxin levels below 1.5 EU\/mL to ensure suitability for sensitive cell culture applications.\u003c\/p\u003e\n      \u003c\/li\u003e\n    \u003c\/ul\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Applications --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eApplications\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-grid\"\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eOrganoid Culture and Embedding\u003c\/h4\u003e\n        \u003cp\u003eProvides a three-dimensional extracellular matrix scaffold for the construction and long-term culture of organoids.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eStem Cell Differentiation Studies\u003c\/h4\u003e\n        \u003cp\u003eSupports differentiation of stem cells in physiologically relevant extracellular matrix conditions.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eAngiogenesis Research\u003c\/h4\u003e\n        \u003cp\u003eApplicable for studying endothelial cell migration, vascular formation, and microenvironment interactions.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eCell Migration and Invasion Assays\u003c\/h4\u003e\n        \u003cp\u003eSuitable for modeling tumor invasion, migration behavior, and cell–matrix interactions.\u003c\/p\u003e\n      \u003c\/div\u003e\n      \u003cdiv class=\"biofargo-app-card\"\u003e\n        \u003ch4\u003eTissue Engineering and Regenerative Biology\u003c\/h4\u003e\n        \u003cp\u003eUsed in experimental systems requiring extracellular matrix simulation for tissue modeling and regenerative research.\u003c\/p\u003e\n      \u003c\/div\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Storage --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eStorage\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-card\"\u003e\n      \u003cp style=\"margin:0;\"\u003eStore at −20 °C or −80 °C for long-term preservation. Avoid repeated freeze–thaw cycles.\u003c\/p\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n  \u003c!-- Documents --\u003e\n  \u003cdiv class=\"biofargo-section\"\u003e\n    \u003ch2 class=\"biofargo-h2\"\u003eDocuments\u003c\/h2\u003e\n    \u003cdiv class=\"biofargo-docs\"\u003e\n      \u003ca class=\"biofargo-btn primary\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Data_Sheet-AMMS_Organoid_Matrix_Gel_Instructions.pdf?v=1773298007\" target=\"_blank\" rel=\"noopener\"\u003e📄 AS-41-5 Data Sheet\u003c\/a\u003e\n      \u003ca class=\"biofargo-btn outline\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/SDS-AMMS_Organoid-specific_Matrigel.pdf?v=1773298007\" target=\"_blank\" rel=\"noopener\"\u003e📄 AS-41-5 SDS\u003c\/a\u003e\n      \u003ca class=\"biofargo-btn outline\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA-MED2408001.pdf?v=1773298007\" target=\"_blank\" rel=\"noopener\"\u003e📄 AS-41-5 COA\u003c\/a\u003e\n    \u003c\/div\u003e\n  \u003c\/div\u003e\n\n\u003c\/div\u003e","brand":"T\u0026L","offers":[{"title":"5mL","offer_id":51379766788277,"sku":"AS-41-5","price":220.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/IMG-AS-41-5.jpg?v=1773298278"},{"product_id":"insure-dna-transfection-reagent","title":"InSure DNA Transfection Reagent","description":"\u003ch2\u003eApplications\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eDNA transfection into cells\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"T\u0026L","offers":[{"title":"1mL","offer_id":51379899596981,"sku":"AS-50-01","price":145.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/AS-50-01.png?v=1753174028"},{"product_id":"trypsin-edta-solution","title":"Trypsin-EDTA Solution","description":"\u003cp\u003e\u003cstrong\u003eSynonym(s): \u003c\/strong\u003eCocoonase, Tryptar, Tryptase\u003c\/p\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eTrypsin consists of a single-chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiological Source\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePorcine\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eQuality Level\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e400\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterility\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterile-filtered\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Line\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBioReagent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eForm\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eSolution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e23.4 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eConcentration\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.25%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTechniques\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eCell culture (mammalian): suitable; Single cell analysis: suitable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eImpurities\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePorcine parvovirus: none detected (9 CFR)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003epH\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e7.0 – 7.6\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShipping Condition\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eDry ice\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage Temperature\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e−20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\u003cp\u003eThe typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.\u003c\/p\u003e\n\u003cp\u003eTrypsin-EDTA solution was used:\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ein detaching HT29 human colorectal cancer cells cultured in RPMI 1640 which was supplemented with 10 % fetal calf serum, during relative cell frequency determination of high concentration samples.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eto trypsinize the transient transfected human embryonic kidney tcA-201 cell line.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eto enzymatically release mouse fibroblasts cells (cell line L929) adhered to the scaffold, during cell culturing to assess the influence of several modified treatments of Poly(L\/D)lactide 96\/4 non-woven scaffolds and fibres.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eto dissociate cells from the culture dish for flow cytometry analysis.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eSuitable for use in the preparation of single cell suspension for sequencing.\u003c\/p\u003e\n\u003ch2\u003eBiochem\/physiol Actions\u003c\/h2\u003e\n\u003cp\u003eTrypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity.\u003c\/p\u003e\n\u003cp\u003eSerine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.\u003c\/p\u003e\n\u003ch2\u003ePreparation Note\u003c\/h2\u003e\n\u003cp\u003eThis product does contain phenol red. Due to shipment on dry ice, there could be significant carbon dioxide buildup in the package. This CO2 may enter the solution and lower the pH slightly, giving an orange rather than pinkish color. The orange solution will still be suitable for use, or the pH can be adjusted with sodium hydroxide. Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containing no Ca\u003csup\u003e2+\u003c\/sup\u003e or Mg\u003csup\u003e2+\u003c\/sup\u003e.\u003c\/p\u003e\n\u003ch2\u003eOther Notes\u003c\/h2\u003e\n\u003cp\u003eTrypsin Solution (2.5 g\/l porcine trypsin and 0.2 g\/l EDTA•4Na in Hank′s Balanced Salt Solution with phenol red, 1X, cell culture tested)\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eThis product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/t4049.pdf?v=1776405131\"\u003e\u003cstrong\u003eSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\"\u003e\u003cstrong\u003eRequest COA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003etrypsin\u003c\/div\u003e","brand":"sigma","offers":[{"title":"500mL","offer_id":52439436656821,"sku":"T4049","price":65.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/3_bf86529a-56d9-4016-a3a7-988a8de4912d.jpg?v=1776411873"},{"product_id":"recombinant-human-il17a","title":"Recombinant Human IL17A","description":"","brand":"Cell Biologics","offers":[{"title":"500 µg","offer_id":52481214218421,"sku":"Z100469","price":600.0,"currency_code":"USD","in_stock":true},{"title":"500 µg (International)","offer_id":52481214251189,"sku":"Z100469-1","price":900.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_61fa5edf-f60b-412f-abfc-e50f5e920a70.jpg?v=1778257067"},{"product_id":"recombinant-human-il18","title":"Recombinant Human IL18","description":"","brand":"Cell Biologics","offers":[{"title":"25 µg","offer_id":52481214644405,"sku":"Z100475","price":70.0,"currency_code":"USD","in_stock":true},{"title":"25 µg (International)","offer_id":52481214677173,"sku":"Z100475-1","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_9a0a3a5d-9891-49f6-962f-f9de614ff2c3.jpg?v=1778254286"},{"product_id":"recombinant-human-il18-1","title":"Recombinant Human IL18","description":"","brand":"Cell Biologics","offers":[{"title":"100 µg","offer_id":52481214808245,"sku":"Z100477","price":210.0,"currency_code":"USD","in_stock":true},{"title":"100 µg (International)","offer_id":52481214841013,"sku":"Z100477-1","price":315.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_0f25ab25-13b0-4556-a2e9-d3562f85cece.jpg?v=1778254277"},{"product_id":"recombinant-human-il18-2","title":"Recombinant Human IL18","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481214873781,"sku":"Z100479","price":2520.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481214906549,"sku":"Z100479-1","price":3780.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_ad00d29c-329c-44a1-ba6a-fd378558eae2.jpg?v=1778254281"},{"product_id":"recombinant-human-il2","title":"Recombinant Human IL2","description":"","brand":"Cell Biologics","offers":[{"title":"50 µg","offer_id":52481216282805,"sku":"Z100485","price":70.0,"currency_code":"USD","in_stock":true},{"title":"50 µg (International)","offer_id":52481216315573,"sku":"Z100485-1","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_00067f91-dce2-4727-a037-ba90591d032f.jpg?v=1778256178"},{"product_id":"recombinant-human-il2-1","title":"Recombinant Human IL2","description":"","brand":"Cell Biologics","offers":[{"title":"100 µg","offer_id":52481216512181,"sku":"Z100487","price":120.0,"currency_code":"USD","in_stock":true},{"title":"100 µg (International)","offer_id":52481216544949,"sku":"Z100487-1","price":180.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_f85980aa-8d3e-4bd0-8db7-c1e18a780539.jpg?v=1778256173"},{"product_id":"recombinant-human-il2-2","title":"Recombinant Human IL2","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481216610485,"sku":"Z100489","price":510.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481216643253,"sku":"Z100489-1","price":765.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_cb640f32-87bf-47e7-8d93-9ce346d9793a.jpg?v=1778256168"},{"product_id":"recombinant-human-opg-tnfrsf11b","title":"Recombinant Human OPG (TNFRSF11B)","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481217691829,"sku":"Z101779","price":1350.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481217724597,"sku":"Z101779-1","price":2025.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_c960afd9-a60c-4e26-9e77-1bcb36d0fade.jpg?v=1778257110"},{"product_id":"recombinant-human-il13","title":"Recombinant Human IL13","description":"","brand":"Cell Biologics","offers":[{"title":"100 µg","offer_id":52481217790133,"sku":"Z100447","price":395.0,"currency_code":"USD","in_stock":true},{"title":"100 µg (International)","offer_id":52481217822901,"sku":"Z100447-1","price":592.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_ecddff98-c70f-46a0-b610-e39ab0112248.jpg?v=1778254258"},{"product_id":"recombinant-human-il13-1","title":"Recombinant Human IL13","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481217888437,"sku":"Z100449","price":3276.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481217921205,"sku":"Z100449-1","price":4914.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_f1ba25d5-6faf-4519-9d63-972304c0a6cb.jpg?v=1778254263"},{"product_id":"recombinant-human-il15","title":"Recombinant Human IL15","description":"","brand":"Cell Biologics","offers":[{"title":"10 µg","offer_id":52481218347189,"sku":"Z100455","price":130.0,"currency_code":"USD","in_stock":true},{"title":"10 µg (International)","offer_id":52481218379957,"sku":"Z100455-1","price":195.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_50f69f8e-071a-4c6a-8eaf-094fadb9c20b.jpg?v=1778254268"},{"product_id":"recombinant-human-pdgfc","title":"Recombinant Human PDGFC","description":"","brand":"Cell Biologics","offers":[{"title":"20 µg","offer_id":52481218511029,"sku":"Z101785","price":70.0,"currency_code":"USD","in_stock":true},{"title":"20 µg (International)","offer_id":52481218543797,"sku":"Z101785-1","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_163c2ab9-56f4-427b-a274-d3eff55ed791.jpg?v=1778255359"},{"product_id":"recombinant-human-il15-1","title":"Recombinant Human IL15","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481219428533,"sku":"Z100459","price":2700.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481219461301,"sku":"Z100459-1","price":4050.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_ea9bdad0-2430-4b9e-9b50-b0fb1586c0a2.jpg?v=1778254272"},{"product_id":"recombinant-human-chemerin","title":"Recombinant Human Chemerin","description":"","brand":"Cell Biologics","offers":[{"title":"1.0 mg","offer_id":52481248952501,"sku":"Z102209","price":2700.0,"currency_code":"USD","in_stock":true},{"title":"1.0 mg (International)","offer_id":52481248985269,"sku":"Z102209-1","price":4050.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_1e1684d7-ebcb-4b7b-abf5-961179804b01.jpg?v=1778256218"},{"product_id":"recombinant-human-il36g","title":"Recombinant Human IL36G","description":"","brand":"Cell Biologics","offers":[{"title":"100 µg","offer_id":52481249771701,"sku":"Z102217","price":300.0,"currency_code":"USD","in_stock":true},{"title":"100 µg (International)","offer_id":52481249804469,"sku":"Z102217-1","price":450.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/22012Medium-Kits_010-500x500_532098e2-eb35-4d79-ada2-cf8c21a123b4.jpg?v=1778255373"},{"product_id":"recombinant-human-il36g-1","title":"Recombinant Human 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