{"title":"Cell Culture Flasks","description":"\u003csection\u003e\n\u003ch1\u003eCell Culture Flasks (TC-Treated \u0026amp; Non-Treated, Sterile, Vented Cap)\u003c\/h1\u003e\n\u003cp\u003eBrowse our selection of \u003cstrong\u003ecell culture flasks\u003c\/strong\u003e designed for reliable cell handling in CO₂ incubators. This collection includes both \u003cstrong\u003eTC-treated\u003c\/strong\u003e and \u003cstrong\u003enon-treated\u003c\/strong\u003e surfaces, along with \u003cstrong\u003esterile\u003c\/strong\u003e packaging and \u003cstrong\u003evented (filter) caps\u003c\/strong\u003e to support consistent workflows across different cell culture needs.\u003c\/p\u003e\n\u003cp\u003eChoose \u003cstrong\u003eTC-treated flasks\u003c\/strong\u003e for adherent cell attachment and routine growth, or select \u003cstrong\u003enon-treated flasks\u003c\/strong\u003e for suspension culture, mixing, and specialized applications where surface attachment is not required.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eQuick Pick: Choose by Surface Type\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTC-Treated Cell Culture Flasks\u003c\/strong\u003e – for adherent cell attachment and consistent growth\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNon-Treated Cell Culture Flasks\u003c\/strong\u003e – for suspension culture and applications where low attachment is preferred\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"margin: 12px 0; padding: 12px; border: 1px solid #e5e5e5; border-radius: 8px;\"\u003e\n\u003ch2 style=\"margin-top: 0;\"\u003eWhat makes these flasks a better choice?\u003c\/h2\u003e\n\u003cul style=\"margin-bottom: 0;\"\u003e\n\u003cli\u003e\n\u003cstrong\u003eTwo surface options\u003c\/strong\u003e: TC-treated or non-treated to match your cell culture workflow\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eVented (filter) caps\u003c\/strong\u003e to support gas exchange in CO₂ incubators\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSterile, ready-to-use\u003c\/strong\u003e packaging to support aseptic workflows\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eOptically clear polystyrene\u003c\/strong\u003e for easy microscopic observation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAngled neck design\u003c\/strong\u003e for improved pipette and cell scraper access\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStackable format\u003c\/strong\u003e to organize incubator space efficiently\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003ch2\u003eQuick Pick: Choose by Flask Format\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eT25 Cell Culture Flasks (25 cm²)\u003c\/strong\u003e – starter cultures, cloning workflows, small-scale experiments \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eT75 Cell Culture Flasks (75 cm²)\u003c\/strong\u003e – routine expansion and general-purpose cell culture \u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eT175 Cell Culture Flasks (175 cm²)\u003c\/strong\u003e – higher-yield growth and scale-up workflows \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eHow to Choose the Right Cell Culture Flask\u003c\/h2\u003e\n\u003ch3\u003e1) Choose the Surface: TC-Treated vs Non-Treated\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTC-treated\u003c\/strong\u003e: Designed to support adherent cell attachment and consistent growth for many common adherent cell lines.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eNon-treated\u003c\/strong\u003e: Preferred for suspension culture or workflows where minimal surface attachment is desired, including mixing, storage, and specialized protocols.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e2) Choose the Size: Growth Area \u0026amp; Throughput\u003c\/h3\u003e\n\u003cp\u003eSelect the flask format based on your target yield, incubator space, and passaging schedule. Larger growth areas can reduce the number of vessels needed during scale-up, while smaller flasks are convenient for controlled runs.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eT25 (25 cm²)\u003c\/strong\u003e: Starter cultures and small-scale experiments\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eT75 (75 cm²)\u003c\/strong\u003e: Routine culture and everyday expansion\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eT175 (175 cm²)\u003c\/strong\u003e: Scale-up and higher-yield growth\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eCollection Highlights\u003c\/h2\u003e\n\u003ch3\u003eVented Cap for Reliable Gas Exchange\u003c\/h3\u003e\n\u003cp\u003eVented (filter) caps support continuous gas exchange in CO₂ incubators, helping maintain suitable culture conditions during extended incubation and routine workflows.\u003c\/p\u003e\n\u003ch3\u003eAngled Neck for Easier Handling\u003c\/h3\u003e\n\u003cp\u003eThe angled neck design improves access for pipettes and cell scrapers, making feeding and harvesting steps more efficient and reducing handling errors.\u003c\/p\u003e\n\u003ch3\u003eClear Polystyrene for Easy Observation\u003c\/h3\u003e\n\u003cp\u003eOptically clear construction supports routine microscopic observation and quick visual checks of culture condition and cell morphology.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eBest-Fit Applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdherent cell culture\u003c\/strong\u003e using TC-treated surfaces for attachment-dependent lines\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSuspension culture\u003c\/strong\u003e and low-attachment workflows using non-treated surfaces\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCell expansion\u003c\/strong\u003e for downstream assays, imaging, and sample preparation\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDrug screening\u003c\/strong\u003e and toxicity assays\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGeneral lab workflows\u003c\/strong\u003e requiring sterile, vented-cap flasks\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003chr\u003e\n\u003ch2\u003eCompare Flask Formats (T25 vs T75 vs T175)\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse;\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align: left; padding: 10px; border-bottom: 1px solid #ddd;\"\u003eFeature\u003c\/th\u003e\n\u003cth style=\"text-align: left; padding: 10px; border-bottom: 1px solid #ddd;\"\u003eT25\u003c\/th\u003e\n\u003cth style=\"text-align: left; padding: 10px; border-bottom: 1px solid #ddd;\"\u003eT75\u003c\/th\u003e\n\u003cth style=\"text-align: left; padding: 10px; border-bottom: 1px solid #ddd;\"\u003eT175\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e\u003cstrong\u003eGrowth area\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e25 cm²\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e75 cm²\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e175 cm²\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e\u003cstrong\u003eBest for\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eStarter cultures, controlled experiments\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eRoutine culture, everyday expansion\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eScale-up and higher-yield growth\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e\u003cstrong\u003eSurface options\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eTC-treated or non-treated\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eTC-treated or non-treated\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eTC-treated or non-treated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003e\u003cstrong\u003eCap\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eVented (filter) cap\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eVented (filter) cap\u003c\/td\u003e\n\u003ctd style=\"padding: 10px; border-bottom: 1px solid #f0f0f0;\"\u003eVented (filter) cap\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 10px;\"\u003e\u003cstrong\u003eMaterial\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePolystyrene\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePolystyrene\u003c\/td\u003e\n\u003ctd style=\"padding: 10px;\"\u003ePolystyrene\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin: 14px 0; padding: 12px; background: #fafafa; border: 1px solid #eee; border-radius: 8px;\"\u003e\n\u003cp style=\"margin: 0;\"\u003e\u003cstrong\u003eTip:\u003c\/strong\u003e If you culture adherent cells, start with \u003cstrong\u003eTC-treated\u003c\/strong\u003e flasks. If you culture suspension cells or need minimal attachment, choose \u003cstrong\u003enon-treated\u003c\/strong\u003e flasks. Then select T25\/T75\/T175 based on target yield and incubator space.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003chr\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003ch3\u003eWhat is the difference between TC-treated and non-treated flasks?\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003eTC-treated\u003c\/strong\u003e flasks are designed to support adherent cell attachment and growth. \u003cstrong\u003eNon-treated\u003c\/strong\u003e flasks are preferred for suspension culture or workflows where minimal attachment is desired.\u003c\/p\u003e\n\u003ch3\u003eWhat does a vented (filter) cap do?\u003c\/h3\u003e\n\u003cp\u003eA \u003cstrong\u003evented (filter) cap\u003c\/strong\u003e supports gas exchange in CO₂ incubators while helping reduce contamination risk during incubation.\u003c\/p\u003e\n\u003ch3\u003eWhich flask size should I choose?\u003c\/h3\u003e\n\u003cp\u003eChoose \u003cstrong\u003eT25\u003c\/strong\u003e for starter cultures and small-scale work, \u003cstrong\u003eT75\u003c\/strong\u003e for routine expansion, and \u003cstrong\u003eT175\u003c\/strong\u003e for scale-up and higher-yield growth.\u003c\/p\u003e\n\u003ch3\u003eAre these flasks sterile?\u003c\/h3\u003e\n\u003cp\u003eYes, products in this collection are supplied \u003cstrong\u003esterile\u003c\/strong\u003e and ready to use for aseptic workflows.\u003c\/p\u003e\n\u003chr\u003e\n\u003ch2\u003eShop This Collection\u003c\/h2\u003e\n\u003cp\u003eBrowse all \u003cstrong\u003ecell culture flasks\u003c\/strong\u003e in this collection to find the right surface and format for your workflow. Use consistent flask formats across your lab to simplify training, inventory, and ordering.\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"REPLACE_WITH_COLLECTION_URL\"\u003eView All Cell Culture Flasks in This Collection\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e","products":[{"product_id":"whb-ccf75-1","title":"T75 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2\u003cmeta charset=\"UTF-8\"\u003eμm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-cell-culture-flasks-tc-treated-vented-cap-whb-ccf25-1-ccf75-1-ccf175-1?variant=41407698534581\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/whb-ccf75-1?variant=41407679266997\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-t75-cell-culture-flasks-tc-treated-vented-cap-whb-ccf75-1?variant=41407689457845\"\u003eT175\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse; height: 237px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: PP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 75cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 250mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"3\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T75 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T75 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 75 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T75 flask?\u003c\/summary\u003e\n\u003cp\u003eA T75 flask provides a growth surface area of 75 cm² and a maximum volume of 250 mL. This size is commonly used for routine cell expansion and offers a balance between culture capacity and laboratory handling convenience.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T75 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 250 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this T75 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T75 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these T75 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T75 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT75 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"WHB","offers":[{"title":"50Pcs\/Case","offer_id":41407679266997,"sku":"WHB-CCF75-1-CSS","price":85.0,"currency_code":"USD","in_stock":true},{"title":"100Pcs\/Case","offer_id":40950534865077,"sku":"WHB-CCF75-1-CS","price":169.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/CellCultureFlasks1.png?v=1732884257"},{"product_id":"t25-cell-culture-flasks-tc-treated-vented-cap-sterile","title":"T25 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2\u003cmeta charset=\"UTF-8\"\u003eμm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-cell-culture-flasks-tc-treated-vented-cap-whb-ccf25-1-ccf75-1-ccf175-1?variant=41407698534581\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/whb-ccf75-1?variant=41407679266997\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-t75-cell-culture-flasks-tc-treated-vented-cap-whb-ccf75-1?variant=41407689457845\"\u003eT175\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: PP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 25cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T25 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T25 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 25 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T25 flask?\u003c\/summary\u003e\n\u003cp\u003eA T25 flask provides a growth surface area of 25 cm² and a maximum volume of 50 mL. This size is commonly used for routine cell culture and small-scale cell expansion while offering convenient handling in the laboratory.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T25 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 50 mL, most cell culture protocols recommend using approximately 5–10 mL of culture medium in a T25 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan this T25 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T25 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T25 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T25 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan these T25 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T25 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT25 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eT25,T25 flask,t-25 flask\u003c\/div\u003e","brand":"WHB","offers":[{"title":"10Pcs\/Pack","offer_id":41261789642933,"sku":"WHB-CCF25-1","price":12.0,"currency_code":"USD","in_stock":true},{"title":"100Pcs\/Case","offer_id":41407698534581,"sku":"WHB-CCF25-1-CSS","price":117.0,"currency_code":"USD","in_stock":true},{"title":"200Pcs\/Case","offer_id":41261789675701,"sku":"WHB-CCF25-1-CS","price":234.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/CellCultureFlasks1_7b775d38-9fc5-4fe4-a9d3-98963332a7ec.png?v=1732884231"},{"product_id":"t175-cell-culture-flasks-tc-treated-vented-cap-sterile","title":"T175 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003e\u003cspan\u003eDescription\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2\u003cmeta charset=\"UTF-8\"\u003eμm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-cell-culture-flasks-tc-treated-vented-cap-whb-ccf25-1-ccf75-1-ccf175-1?variant=41407698534581\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/whb-ccf75-1?variant=41407679266997\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/copy-of-t75-cell-culture-flasks-tc-treated-vented-cap-whb-ccf75-1?variant=41407689457845\"\u003eT175\u003c\/a\u003e.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: PP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 175cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 600mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T175 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T175 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 175 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T175 flask?\u003c\/summary\u003e\n\u003cp\u003eA T175 flask provides a growth surface area of 175 cm² and a maximum volume of 600 mL. This size is commonly used for large-scale cell expansion and offers high culture capacity while maintaining convenient laboratory handling.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T175 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 600 mL, most cell culture protocols recommend using approximately 35–55 mL of culture medium in a T175 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan this T175 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T175 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan these T175 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T175 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT175 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"WHB","offers":[{"title":"5Pcs\/Pack","offer_id":41261831880885,"sku":"WHB-CC175-1","price":38.0,"currency_code":"USD","in_stock":true},{"title":"20Pcs\/Case","offer_id":41407689457845,"sku":"WHB-CC175-1-CSS","price":150.0,"currency_code":"USD","in_stock":true},{"title":"40Pcs\/Case","offer_id":41261831913653,"sku":"WHB-CC175-1-CS","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/CellCultureFlasks1_286031ad-bc59-402a-8cea-c223b83104de.png?v=1732884205"},{"product_id":"cell-culture-flask","title":"T25 Cell Culture Flasks with Vented Cap NonTreated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flask?variant=44836252876981\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-75-cm2-175ml-white-cap-xented-biofargo?variant=44836244848821\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-175-cm2-550ml-white-cap-xented-biofargo?variant=44836259037365\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: White\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 25cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eNon-Treated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eNot TC-Treated (Suspension Treatment):\u003c\/p\u003e\n\u003cp\u003eUse Scenario: Suitable for suspension cell culture, offering an adjustable culture environment and convenient operation.\u003c\/p\u003e\n\u003cp\u003eFeatures: Non-TC-treated containers are ideal for maintaining the growth and reproduction of suspended cells.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T25 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T25 cell culture flask is designed for the growth, maintenance, and expansion of suspension mammalian cells. With a 25 cm² growth surface, it is commonly used for routine suspension cell culture, cell line maintenance, protein expression, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat does Non-Treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eNon-Treated refers to a cell culture flask without tissue culture surface treatment. The untreated hydrophobic surface is suitable for suspension cell cultures or applications where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T25 flask?\u003c\/summary\u003e\n\u003cp\u003eA T25 flask provides a growth surface area of 25 cm² and a maximum volume of 50 mL. This size is commonly used for routine suspension cell culture and offers convenient handling in the laboratory.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T25 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 50 mL, most cell culture protocols recommend using approximately 5–10 mL of culture medium in a T25 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan this T25 flask be used for suspension cell culture?\u003c\/summary\u003e\n\u003cp\u003eYes. The non-treated surface is suitable for suspension cell lines that do not require attachment for growth. It is commonly used for suspension mammalian cells and other applications where cell adhesion is unnecessary.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T25 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T25 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T25 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell cultures. The cap is made of HDPE.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan these T25 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T25 non-treated cell culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT25 non-treated cell culture flasks are widely used for suspension cell culture, hybridoma culture, protein expression, bioprocess development, cell maintenance, and general life science research where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eT25,T25 flask,t-25 flask\u003c\/div\u003e","brand":"Cellprobio","offers":[{"title":"25Bags\/Case(300Pcs\/Case)","offer_id":44836252876981,"sku":"N803025","price":228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DSC_0261.jpg?v=1757474912"},{"product_id":"cell-culture-flasks-sterile-surface-area-75-cm2-175ml-white-cap-xented-biofargo","title":"T75 Cell Culture Flasks with Vented Cap NonTreated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer a large culture area and promote growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large marking areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flask?variant=44836252876981\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-75-cm2-175ml-white-cap-xented-biofargo?variant=44836244848821\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-175-cm2-550ml-white-cap-xented-biofargo?variant=44836259037365\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: White\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 75cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 175mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eNon-Treated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"2\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable characteristics in accordance with valid specifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eNot TC-Treated (Suspension Treatment):\u003c\/p\u003e\n\u003cp\u003eUse Scenario: Suitable for suspension cell culture, offering an adjustable culture environment and convenient operation.\u003c\/p\u003e\n\u003cp\u003eFeatures: Non-TC-treated containers are ideal for maintaining the growth and reproduction of suspended cells.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T75 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T75 cell culture flask is designed for the growth, maintenance, and expansion of suspension mammalian cells. With a 75 cm² growth surface, it is commonly used for routine suspension cell culture, cell line maintenance, protein expression, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat does Non-Treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eNon-Treated refers to a cell culture flask without tissue culture surface treatment. The untreated hydrophobic surface is suitable for suspension cell cultures or applications where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T75 flask?\u003c\/summary\u003e\n\u003cp\u003eA T75 flask provides a growth surface area of 75 cm² and a maximum volume of 175 mL. This size is commonly used for routine suspension cell culture and offers a balance between culture capacity and laboratory handling convenience.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T75 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 175 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan this T75 flask be used for suspension cell culture?\u003c\/summary\u003e\n\u003cp\u003eYes. The non-treated surface is suitable for suspension cell lines that do not require attachment for growth. It is commonly used for suspension mammalian cells and other applications where cell adhesion is unnecessary.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T75 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell cultures. The cap is made of HDPE.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan these T75 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T75 non-treated cell culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT75 non-treated cell culture flasks are widely used for suspension cell culture, hybridoma culture, protein expression, bioprocess development, cell maintenance, and general life science research where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"Cellprobio","offers":[{"title":"18Bags\/Case(90Pcs\/Case)","offer_id":44836244848821,"sku":"N803075","price":153.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DSC_0261.jpg?v=1757474912"},{"product_id":"cell-culture-flasks-sterile-surface-area-175-cm2-550ml-white-cap-xented-biofargo","title":"T175 Cell Culture Flasks with Vented Cap NonTreated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flask?variant=44836252876981\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-75-cm2-175ml-white-cap-xented-biofargo?variant=44836244848821\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/collections\/cell-culture-flasks\/products\/cell-culture-flasks-sterile-surface-area-175-cm2-550ml-white-cap-xented-biofargo?variant=44836259037365\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: White\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 175cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 550mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eNon-Treated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\u003cspan\u003eFeatures\u003c\/span\u003e\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eNot TC-Treated (Suspension Treatment):\u003c\/p\u003e\n\u003cp\u003eUse Scenario: Suitable for suspension cell culture, offering an adjustable culture environment and convenient operation.\u003c\/p\u003e\n\u003cp\u003eFeatures: Non-TC-treated containers are ideal for maintaining the growth and reproduction of suspended cells.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T175 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T175 cell culture flask is designed for the growth, maintenance, and expansion of suspension mammalian cells. With a 175 cm² growth surface, it is commonly used for routine suspension cell culture, cell line maintenance, protein expression, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat does Non-Treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eNon-Treated refers to a cell culture flask without tissue culture surface treatment. The untreated hydrophobic surface is suitable for suspension cell cultures or applications where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T175 flask?\u003c\/summary\u003e\n\u003cp\u003eA T175 flask provides a growth surface area of 175 cm² and a maximum volume of 550 mL. This size is commonly used for large-scale suspension cell culture and offers high culture capacity while maintaining convenient laboratory handling.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T175 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 550 mL, most cell culture protocols recommend using approximately 35–55 mL of culture medium in a T175 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan this T175 flask be used for suspension cell culture?\u003c\/summary\u003e\n\u003cp\u003eYes. The non-treated surface is suitable for suspension cell lines that do not require attachment for growth. It is commonly used for suspension mammalian cells and other applications where cell adhesion is unnecessary.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T175 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell cultures. The cap is made of HDPE.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eCan these T175 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T175 non-treated cell culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT175 non-treated cell culture flasks are widely used for suspension cell culture, hybridoma culture, protein expression, bioprocess development, cell maintenance, and general life science research where cell attachment is not required.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"Cellprobio","offers":[{"title":"10Bags\/Case(50Pcs\/Case)","offer_id":44836259037365,"sku":"N803175","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DSC_0261.jpg?v=1757474912"},{"product_id":"t25-cell-culture-flask-with-vent-cap-tc-treated-sterile","title":"T25 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer large culture area and promotes growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/products\/t25-cell-culture-flask-with-vent-cap-tc-treatedm-steriled?variant=51539735806133\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/products\/t75-cell-culture-flask-with-seal-cap-tc-treated-steriled?variant=51539739082933\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/products\/t175-cell-culture-flask-with-vent-cap-tc-treated-steriled?variant=51539749929141\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 25cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 50mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"3\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T75 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T75 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 75 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T75 flask?\u003c\/summary\u003e\n\u003cp\u003eA T75 flask provides a growth surface area of 75 cm² and a maximum volume of 245 mL. This size is commonly used for routine cell expansion and offers a balance between culture capacity and laboratory handling convenience.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T75 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 245 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this T75 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T75 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 Cell Culture Flasks are sterilized by irradiation and supplied in self-sealing sterile bags, ready for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), while the cap is manufactured from high-density polyethylene (HDPE), providing excellent visibility for cell observation and reliable sealing performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these T75 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T75 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT75 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display: none;\"\u003eT25,T25 flask,t-25 flask\u003c\/div\u003e","brand":"Cellprobio","offers":[{"title":"25Packs\/Case(300Pcs\/Case)","offer_id":51539735806133,"sku":"803025","price":228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250908_1.jpg?v=1757474411"},{"product_id":"t75-cell-culture-flask-with-seal-cap-tc-treated-sterile","title":"T75 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer a large culture area and promote growth. Vented caps with a 0.2 μm filter promote ventilation and prevent contamination. The angled neck provides fast and easy access. Meticulous manufacturing process guarantees reliability for your cell culture work. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/products\/t25-cell-culture-flask-with-vent-cap-tc-treated-steriled?variant=51539735806133\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/products\/t75-cell-culture-flask-with-seal-cap-tc-treated-steriled?variant=51539739082933\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/products\/t175-cell-culture-flask-with-vent-cap-tc-treated-steriled?Angled%20neck%20provides%20fast%20and%20easy%20access.variant=51539749929141\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse; height: 237px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd rowspan=\"2\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 75cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 245mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd rowspan=\"3\" style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003echaracteristics in accordance with the valid specification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cspan\u003e0.2 μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eSterilized self-sealing bag to prevent contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: TC-treated cell culture flasks offer strong adhesion properties, making them ideal for long-term cell culture and large-scale cell expansion.\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T75 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T75 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 75 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T75 flask?\u003c\/summary\u003e\n\u003cp\u003eA T75 flask provides a growth surface area of 75 cm² and a maximum volume of 245 mL. This size is commonly used for routine cell expansion and offers a balance between culture capacity and laboratory handling convenience.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T75 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 245 mL, most cell culture protocols recommend using approximately 15–30 mL of culture medium in a T75 flask. The optimal volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this T75 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T75 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 Cell Culture Flasks are individually packaged and sterilized by irradiation. The flasks are supplied ready to use and are suitable for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T75 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), providing excellent visibility for microscopic observation and routine monitoring of cell morphology and confluency.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these T75 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T75 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT75 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"Cellprobio","offers":[{"title":"18Bags\/Case(90Pcs\/Case)","offer_id":51539739082933,"sku":"803075","price":174.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250908_1.jpg?v=1757474411"},{"product_id":"t175-cell-culture-flask-with-vent-cap-tc-treated-sterile","title":"T175 Cell Culture Flasks with Vented Cap TC Treated Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo cell culture flasks feature flat surfaces that offer a large culture area and promote growth. Vented caps with 0.2μm filter promote ventilation and prevent contamination. Angled neck provides fast and easy access. Each flask features two large noting areas that allow quick identification. Each flask features two large noting areas that allow quick identification. Flasks are available in three different sizes: \u003ca href=\"https:\/\/biofargo.com\/products\/t25-cell-culture-flask-with-vent-cap-tc-treatedm-steriled?variant=51539735806133\"\u003eT25\u003c\/a\u003e, \u003ca href=\"https:\/\/biofargo.com\/products\/t75-cell-culture-flask-with-seal-cap-tc-treated-steriled?variant=51539739082933\"\u003eT75\u003c\/a\u003e, and \u003ca href=\"https:\/\/biofargo.com\/products\/t175-cell-culture-flask-with-vent-cap-tc-treated-steriled?variant=51539749929141\"\u003eT175\u003c\/a\u003e.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse; height: 237px;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eColour\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: Red\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\" rowspan=\"2\"\u003eMaterial\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eFlask: PS\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eCap: HDPE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eSterilization\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eIrradiation sterilization\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 21px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 39px;\" rowspan=\"2\"\u003eVolume\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 21px;\"\u003eGrowth area: 175cm\u003csup\u003e2\u003c\/sup\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eMax. volume: 550mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTreatment\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eTC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 18px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 90px;\" rowspan=\"2\"\u003eQuality Control\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 18px;\"\u003eRaw Material-Control: physical testing\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 36px;\"\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle; height: 36px;\"\u003eProduct-Control: testing of attributive and variable characteristics in accordance with valid specifications\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eTC treated\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eVented caps with \u003cmeta charset=\"UTF-8\"\u003e \u003cspan\u003e0.2μm filter, promoting ventilation and preventing contamination.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eAngled bottle neck designed for pipettes and cell scrapers.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eMeasurements and annotation area on both sides.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eSterilized self-sealing bag to prevent contamination.\u003cbr\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eEasily stackable.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cspan\u003eGamma sterilized, DNase-free, RNase-free.\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cp\u003eTC-Treated (Adherent Treatment):\u003c\/p\u003e\n\u003cp\u003eScenario: Suitable for the culture of adherent cells, providing excellent cell adhesion.\u003c\/p\u003e\n\u003cp\u003eEffect: The cell culture flask treated with TC has a good adhesion effect, which is suitable for long-term cell culture and large-scale cell expansion\u003c\/p\u003e\n\u003ch2\u003eFrequently Asked Questions\u003c\/h2\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is a T175 cell culture flask used for?\u003c\/summary\u003e\n\u003cp\u003eA T175 cell culture flask is designed for the growth, maintenance, and expansion of adherent mammalian cells. With a 175 cm² growth surface, it is commonly used for routine cell culture, cell line maintenance, transfection experiments, virus production, and preparation of cells for downstream assays.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat does TC-treated mean on a cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eTC-treated (Tissue Culture Treated) refers to a surface modification process that increases the hydrophilicity of the flask surface. This treatment improves cell attachment and spreading, making TC-treated flasks ideal for adherent cell lines such as HEK293, CHO, Vero, HeLa, and fibroblast cells.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the growth area of a T175 flask?\u003c\/summary\u003e\n\u003cp\u003eA T175 flask provides a growth surface area of 175 cm² and a maximum volume of 550 mL. This size is commonly used for routine cell expansion and large-scale cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails open=\"\"\u003e\n\u003csummary\u003eWhat is the recommended working volume for a T175 cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eAlthough the maximum volume is 550 mL, the optimal working volume depends on the cell type and specific experimental requirements.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat is the difference between a vented cap and a sealed cap cell culture flask?\u003c\/summary\u003e\n\u003cp\u003eA vented cap contains a 0.2 μm filter that allows sterile gas exchange while helping prevent contamination. Vented cap flasks are typically recommended for incubator-based mammalian cell culture. Sealed cap flasks are better suited for transport, storage, or applications where gas exchange is not required.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhy is a 0.2 μm vent filter important in cell culture flasks?\u003c\/summary\u003e\n\u003cp\u003eThe 0.2 μm filter allows carbon dioxide and oxygen exchange while reducing the risk of microbial contamination. Proper gas exchange helps maintain stable culture conditions and supports healthy cell growth in CO₂ incubators.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan this T175 flask be used for HEK293, CHO, Vero, or HeLa cells?\u003c\/summary\u003e\n\u003cp\u003eYes. The TC-treated surface is suitable for a wide range of adherent mammalian cell lines, including HEK293, CHO, Vero, HeLa, MDCK, fibroblasts, stem cells, and many other attachment-dependent cell cultures.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre Biofargo T175 cell culture flasks sterile?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 Cell Culture Flasks are sterilized by irradiation and supplied in self-sealing sterile bags, ready for aseptic cell culture applications.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eAre these cell culture flasks DNase-free and RNase-free?\u003c\/summary\u003e\n\u003cp\u003eYes. Biofargo T175 flasks are manufactured under strict quality control procedures and are supplied DNase-free and RNase-free, helping protect sensitive cell culture and molecular biology workflows.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat material is used to manufacture the flask?\u003c\/summary\u003e\n\u003cp\u003eThe flask body is made from high-clarity polystyrene (PS), while the cap is manufactured from high-density polyethylene (HDPE), providing excellent visibility for cell observation and reliable sealing performance.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eCan these T175 flasks be stacked during incubation?\u003c\/summary\u003e\n\u003cp\u003eYes. The flask design allows stable stacking, helping optimize incubator space while maintaining convenient handling during routine cell culture operations.\u003c\/p\u003e\n\u003c\/details\u003e\n\u003cdetails\u003e\n\u003csummary\u003eWhat types of applications are T175 tissue culture flasks commonly used for?\u003c\/summary\u003e\n\u003cp\u003eT175 tissue culture flasks are widely used for adherent cell culture, cell expansion, transfection studies, recombinant protein expression, virus propagation, vaccine research, stem cell culture, toxicity testing, and general life science research.\u003c\/p\u003e\n\u003c\/details\u003e","brand":"Cellprobio","offers":[{"title":"10Packs\/Case(50Pcs\/Case)","offer_id":51539749929141,"sku":"803175","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/20250908_1.jpg?v=1757474411"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/collections\/Cell_Culture_Flasks.jpg?v=1767839233","url":"https:\/\/biofargo.com\/collections\/cell-culture-flasks.oembed?page=2","provider":"Biofargo","version":"1.0","type":"link"}