{"title":"Cell Culture Contamination Control","description":"","products":[{"product_id":"mycoplasma-clearing-reagent-iibo311-002","title":"mycoplasma clearing reagent II(BO311-002)","description":"Concentration of mycoplasma scavenging reagent II： 100 x\u003cbr\u003e\u003cbr\u003eMycoplasma scavenging reagents contain drugs that inhibit mycoplasma protein synthesis and inhibit mycoplasma DNA synthesis. It only needs to be treated for 7-14 days to achieve the goal of permanent killing and removal of mycoplasma. At present, this product has been verified on more than 20 kinds of cells (such as 293T, Cho-k1, SP2\/0, Hela, LX2, ST, Jurkat, HA-VSMC, Min-6, 4T1, etc.), all of which can effectively clear mycoplasma, and no obvious toxic and side effects on cells were found.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eUsage method\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e1. Before use, dilute mycoplasma scavenging reagent Ⅰ by 10 times with PBS and then pack (mycoplasma scavenging reagent II does not need to be diluted and directly pack for use), and store in the refrigerator at 4℃ (If the remaining reagent is not used within a short period of time, it should be frozen at -20℃ to avoid the loss of efficacy). Add to complete medium at 1:100 volume ratio when using.\u003cbr\u003e\u003cbr\u003e2. Using the T25 cell culture bottle as an example, 500,000-1,000,000 cells were spread into the T25 cell culture bottle and 4-6 mL of fresh complete medium containing mycoplasma scavenging reagent was added.\u003cbr\u003e\u003cbr\u003e3. Replace fresh complete medium with mycoplasma scavenging reagent every 2-3 days. If the cells are overgrown or too dense, appropriate amount of cells should be added to a new T25 cell culture flask after passage, and fresh complete medium containing mycoplasma scavenging reagents should be added.\u003cbr\u003e\u003cbr\u003e4. After 7 days of treatment, the mycoplasma detection kit can be used to detect whether the mycoplasma is completely cleared. If there is still residual mycoplasma, continue processing for 3-6 days before testing. It is recommended to retest mycoplasma after 1 month to ensure that there is no new mycoplasma contamination.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePreservation method\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eStore at -20 ℃\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eMatters needing attention\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003eIn the process of using mycoplasma scavenging reagent, if the cell morphology or proliferation rate is found to be changed, the concentration of the scavenging reagent can be appropriately reduced.\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eantimicoplasma\u003c\/div\u003e","brand":"Lonsera","offers":[{"title":"Default Title","offer_id":42793930293429,"sku":"BO311-002","price":973.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/products\/zhiyuanti.png?v=1732877680"},{"product_id":"mycguard-1-solution-100-for-disinfecting-water-bath-of-co2-incubator","title":"MycGuard™ MycGuard-1 Solution (100x) for Disinfecting Water Plate of CO2 Incubator","description":"\u003cp\u003e\u003cstrong\u003enotes：\u003c\/strong\u003e It is non-toxic, non-volatile, and non-corrosive antimicrobial and fungicidal agent used to disinfect water tray in CO2 incubators. \u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMycoplasma contamination not only involves places that directly contact cells such as cell culture plates, culture reagents, or ultra-clean benches but also equipment such as water trays and water baths in cell culture incubators that may cause mycoplasma contamination. In addition to providing scavenging agents that directly remove mycoplasma agents, normal cells are also provided with mycoplasma preventive agents to prevent mycoplasma contamination. It mainly includes the following aspects: reagent prevention (directly added to the cell culture medium), ultra-clean bench prevention (spray), water bath prevention (directly added to the water bath) cell incubator water tray prevention, etc.\u003c\/p\u003e\n\u003cp\u003eJust like Aquaguard-1\u003cspan\u003e from Biological Industry, t\u003c\/span\u003ehis product is a mycoplasma prevention reagent in the water plate of the CO2 incubator. It is provided in the form of a 100× concentrated solution. It can be directly added to the water plate according to the proportion when using it. It is recommended to replace it every 7-10 days.\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003eKey Feature\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad-Spectrum and Highly Effective:\u003c\/strong\u003e Specifically designed to eliminate microbial contamination in water baths, including bacteria, fungi, and mycoplasma, without the risk of bacterial resistance development.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Safety Profile:\u003c\/strong\u003e Non-irritating, non-corrosive, and non-volatile. Completely degrades within 7-10 days. Mouse and rabbit feeding and irritation tests have shown no adverse reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient to Use:\u003c\/strong\u003e Easy to operate, saving time and effort.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstruction\u003c\/h2\u003e\n\u003cp\u003eAdd this product directly to the water plate in CO2 Incubator at a ratio of 1:100, and perform treatment every 7-10 days.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eDocument\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS-40609ES60_1.pdf?v=1699602292\" type=\"application\/pdf\" target=\"_blank\"\u003eMSDS-40609ES60\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MycGuard-1_COA.pdf?v=1734018466\" type=\"application\/pdf\" target=\"_blank\"\u003eCertificate of Analysis\u003c\/a\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003eantimicoplasma\u003c\/div\u003e","brand":"Yeasenbio","offers":[{"title":"100 mL","offer_id":43046806749365,"sku":"40609ES60","price":45.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/DSC_0244.jpg?v=1756374925"},{"product_id":"mycguard-1-solution-1000-for-disinfecting-external-water-bath","title":"MycGuard™ MycGuard-2 Solution (500x), for Disinfecting Ordinary Water Bath","description":"\u003cp\u003e\u003cspan\u003e\u003cstrong\u003eNotes： \u003c\/strong\u003eIt is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths.\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eIntroduction\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eMycoplasma contamination not only involves places that directly contact cells such as cell culture plates, culture reagents, or ultra-clean benches but also equipment such as water trays and water baths in cell culture incubators that may cause mycoplasma contamination. In addition to providing scavenging agents that directly remove mycoplasma agents, normal cells are also provided with mycoplasma preventive agents to prevent mycoplasma contamination. It mainly includes the following aspects: reagent prevention (directly added to the cell culture medium), ultra-clean bench prevention (spray), water bath prevention (directly added to the water bath) cell incubator water tray prevention, etc.\u003c\/p\u003e\n\u003cp\u003eIt is primarily composed of cationic peptide antibiotics, specifically designed to eliminate bacteria, fungi, mycoplasma, and other microorganisms in water baths. Its mechanism of action involves physically breaking down the cell walls of microorganisms and inhibiting the synthesis of enzymes required for their reproduction, ultimately leading to microbial death. As a result, this product does not induce antibiotic resistance. Multiple independent validation tests have shown that this product effectively clears various types of bacteria, fungi, mycoplasma, and other microorganisms. It is especially effective in inhibiting airborne bacteria, fungi, spores, and spore-forming bacteria for up to 7-10 days It is provided in the form of a 500× concentrated solution. It can be directly added to the water bath according to the proportion when using it.\u003c\/p\u003e\n\u003ch2\u003eKey Feature\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eBroad-Spectrum and Highly Effective:\u003c\/strong\u003e Specifically designed to eliminate microbial contamination in water baths, including bacteria, fungi, and mycoplasma, without the risk of bacterial resistance development.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Safety Profile:\u003c\/strong\u003e Non-irritating, non-corrosive, and non-volatile. Completely degrades within 7-10 days. Mouse and rabbit feeding and irritation tests have shown no adverse reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eConvenient to Use:\u003c\/strong\u003e Easy to operate, saving time and effort.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eInstruction\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWear latex gloves during operation.\u003c\/li\u003e\n\u003cli\u003eAdd the product directly to the water bath at a ratio of 1:500, treating every 7-10 days.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003e\u003cspan\u003eStorage\u003c\/span\u003e\u003c\/h2\u003e\n\u003cp\u003e\u003cspan\u003eStore at room temperature\u003c\/span\u003e\u003c\/p\u003e\n\u003cul\u003e\u003c\/ul\u003e\n\u003cdiv id=\"display-search-keyword\" style=\"display:none\"\u003e40610es, antimicoplasma\u003c\/div\u003e","brand":"Yeasenbio","offers":[{"title":"100 mL","offer_id":43256824660149,"sku":"40610ES60","price":109.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/my-2.jpg?v=1756374955"},{"product_id":"100x-penicillin-streptomycin-solution","title":"100X Penicillin-Streptomycin Solution","description":"\u003cstyle\u003e\n  \/* =========================\n     Biofargo Table SOP (Embedded)\n     ========================== *\/\n  .biofargo-wrap .biofargo-table-wrap{\n    overflow-x:auto;\n    -webkit-overflow-scrolling:touch;\n    border:1px solid #eee;\n    border-radius:10px;\n    padding:6px;\n    background:#fff;\n    margin-top:15px;\n  }\n  .biofargo-wrap table.biofargo-table{\n    width:100%;\n    border-collapse:collapse;\n    font-size:14px;\n    min-width:640px;\n    background:#fff;\n  }\n  .biofargo-wrap table.biofargo-table th,\n  .biofargo-wrap table.biofargo-table td{\n    border:1px solid #e9ecef;\n    padding:15px;\n    vertical-align:middle;\n    white-space:normal;\n    word-break:break-word;\n  }\n  .biofargo-wrap table.biofargo-table thead th{\n    background:#f2f2f2;\n    color:#000;\n    font-weight:700;\n    text-align:left;\n  }\n  .biofargo-wrap table.biofargo-table tbody td:first-child{\n    background:#f8f9fa;\n    font-weight:600;\n    width:30%;\n  }\n\u003c\/style\u003e\n\u003cdiv class=\"biofargo-wrap\" style=\"max-width: 950px; margin: 0 auto; font-family: 'Segoe UI', Arial, sans-serif; color: #333; line-height: 1.6; border: 1px solid #eee;\"\u003e\n\u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n\u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e Description\u003c\/h2\u003e\n\u003cdiv style=\"font-size: 15px; color: #444; text-align: justify;\"\u003eBioFargo 100X Penicillin-Streptomycin Solution (commonly known as \"Pen-Strep\") is a broad-spectrum antibiotic mixture designed to prevent bacterial contamination in cell culture. This ready-to-use solution contains 10,000 units\/mL of Penicillin G and 10,000 μg\/mL of Streptomycin sulfate in a 0.85% saline or citrate buffer.\u003cbr\u003e\u003cbr\u003ePenicillin acts by inhibiting bacterial cell wall synthesis (targeting Gram-positive bacteria), while Streptomycin inhibits protein synthesis by binding to the 30S ribosomal subunit (targeting both Gram-negative and Gram-positive bacteria). Our solution is sterile-filtered (0.22 μm) and rigorously tested to ensure optimal cell growth and viability without toxicity at the recommended working concentration.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n\u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e Specification\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\"\u003e\n\u003ctable class=\"biofargo-table\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eFull name\u003c\/td\u003e\n\u003ctd\u003e100X Penicillin-Streptomycin Solution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDescription\u003c\/td\u003e\n\u003ctd\u003ePenicillin-Streptomycin Liquid，this solution is ready to use for cell culture after going over sterilization by filtration. It is necessary to be diluted 100 fold before usage\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eApplications\u003c\/td\u003e\n\u003ctd\u003eCell culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eComponent\u003c\/td\u003e\n\u003ctd\u003ePenicillin 10 KU\/ml；Streptomycin 10 mg\/ml；NaCl 0.9%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n\u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e Features\u003c\/h2\u003e\n\u003cul style=\"margin: 0; padding-left: 20px; font-size: 15px; color: #444;\"\u003e\n\u003cli style=\"margin-bottom: 12px;\"\u003eBroad-Spectrum Protection: Highly effective against a wide range of Gram-positive and Gram-negative bacterial contaminants.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 12px;\"\u003eCell Culture Tested: Validated for use with various primary cells and immortalized cell lines to ensure no adverse effects on morphology or proliferation.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 12px;\"\u003eReady-to-Use 100X Concentrate: Simply dilute 1:100 in your basal medium (e.g., DMEM, RPMI 1640) for a 1X final working concentration.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 12px;\"\u003eSterile \u0026amp; Pure: 0.22 μm membrane filtered; tested for sterility, endotoxin levels, and mycoplasma.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 0;\"\u003eSuperior Stability: Formulated for long-term frozen storage with excellent lot-to-lot consistency.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 30px; border-bottom: 8px solid #f4f6f8;\"\u003e\n\u003ch2 style=\"font-size: 22px; color: #0056b3; margin-top: 0; margin-bottom: 20px; display: flex; align-items: center;\"\u003e\n\u003cspan style=\"width: 12px; height: 12px; background-color: #0056b3; margin-right: 12px;\"\u003e\u003c\/span\u003e Applications\u003c\/h2\u003e\n\u003cdiv style=\"display: grid; grid-template-columns: repeat(auto-fit, minmax(300px, 1fr)); gap: 10px;\"\u003e\n\u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eRoutine Cell Culture: Prevention of bacterial infections in standard laboratory cell maintenance.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003ePrimary Cell Isolation: Safeguard against contamination during the sensitive initial phases of primary culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cul style=\"margin: 0; padding-left: 20px; font-size: 14px; color: #555;\"\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eLarge-Scale Bioprocessing: Maintain sterile conditions in expanded cell populations.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 8px;\"\u003eTissue Engineering: Protection of sensitive scaffolds and tissue constructs from microbial growth.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"padding: 30px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 40px;\"\u003e\n\u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n\u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eComponents\u003c\/h2\u003e\n\u003cdiv style=\"padding: 15px; border: 1px solid #e0e0e0; background-color: #fff; font-size: 14px;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003ePenicillin 10 KU\/ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eStreptomycin 10 mg\/ml\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNaCl 0.9%\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"flex: 1; min-width: 280px;\"\u003e\n\u003ch2 style=\"font-size: 20px; color: #0056b3; margin-top: 0; margin-bottom: 15px;\"\u003eStorage\u003c\/h2\u003e\n\u003cdiv style=\"padding: 15px; border: 1px solid #ffccd5; background-color: #fff5f5; color: #c53030; font-weight: bold; font-size: 18px; border-radius: 4px;\"\u003e-20°C\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Sangon","offers":[{"title":"100ml","offer_id":43838947360949,"sku":"P320018-0100","price":22.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_111e6438-2528-45c6-a319-87e1560e6b12.jpg?v=1767662500"},{"product_id":"penicillin-g-10ku-ml-solution-sterile-biofargo","title":"Penicillin G, 10KU\/ml Solution, Sterile- Biofargo","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo Penicillin G, 10KU\/mL Solution, Sterile is a ready-to-use β-lactam antibiotic solution supplied as a sterile 10 mL vial (10,000 units\/mL) for laboratory research applications. Formulated for short-term storage at -20°C, this sterile solution is intended as a microbiological reagent and cell-culture contamination control agent — not for human or veterinary therapeutic use.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%; border-style: solid;\" border=\"1\" width=\"90%\" cellspacing=\"0\" cellpadding=\"8\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 50%;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 50%;\"\u003e\u003cstrong\u003eValue\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePenicillin G, 10KU\/ml Solution, Sterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eNumber of Containers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eQuantity\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e-20°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRefrigerator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eIce\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDangerous Goods\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e51101500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAntibiotics and Antimycotics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStock Status\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eUsually in Stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eReady-to-use sterile solution: Supplied as a liquid formulation at 10,000 U\/mL in a single 10 mL vial, eliminating preparation steps.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eLaboratory-grade quality: Manufactured under controlled conditions to ensure sterility, consistency, and reliable performance in research settings.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eRefrigerated storage stability: Recommended storage at -20°C; shipped on ice to maintain product integrity.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eEffective antibacterial activity: Commonly used to inhibit Gram-positive bacterial contamination in laboratory and cell culture applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eConvenient packaging \u0026amp; compliance: Non-dangerous-goods classification with UNSPSC code 51101500 for streamlined laboratory procurement.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSupplementing cell culture media to help prevent bacterial contamination when used according to established laboratory protocols.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMicrobiological research, in vitro assays, and antibiotic sensitivity studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eGeneral laboratory use as a sterile penicillin stock solution for research and quality control workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.BS729-NO_LOT.pdf?v=1734078526\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.BS729-Penicillin.pdf?v=1734078538\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Usage Note\u003c\/h2\u003e\n\u003cp\u003eFor research use only. Not intended for human or veterinary therapeutic use. Handle antibiotics in accordance with institutional biosafety guidelines.\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"10mL","offer_id":44907521867957,"sku":"BS729-10mL","price":13.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Penicillin_G_10KU_ml_Solution_Sterile-_Biofargo.jpg?v=1766389324"},{"product_id":"penicillin-streptomycin-amphotericin-b-sterile-biofargo","title":"Penicillin\/Streptomycin\/Amphotericin B, Sterile","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePenicillin Streptomycin Amphotericin B solution is used in cell culture media. When reconstituted in 10ml of sterile water, the concentrations of antibiotic are as followed , Penicillin G, Sodium Salt \u0026gt; 10KU\/ml, Streptomycin Sulfate \u0026gt; 10mg\/ml and Amphotericin B \u0026gt; 250μg\/ml. \u003c\/p\u003e\n\u003cp\u003eIt is effective against yeast, fungi, Gram-positive and Gram-negative bacteria.\u003c\/p\u003e\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e10mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eRefrigerator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eICE\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e51101500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eAntibiotics and Antimycotics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eDG\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003e2 to 8℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"width: 50%; height: 3px; text-align: center; vertical-align: middle;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eDocuments\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.BS733-Pen_strep_amphB.pdf?v=1734080173\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.BS733-Pen_strep_amphB.pdf?v=1734080173\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan style=\"color: rgb(255, 42, 0);\"\u003eDisclaimer: For laboratory research use only.\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"10mL","offer_id":44907610669237,"sku":"BS733-10mL","price":14.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/PenicillinStreptomycinAmphotericinB_sterile-Biofargo.jpg?v=1745223348"},{"product_id":"penicillin-streptomycin-neomycin-sterile-biofargo","title":"Penicillin\/Streptomycin\/Neomycin, Sterile- Biofargo","description":"\u003ch2\u003eSpecification\u003c\/h2\u003e\n\u003ctable cellpadding=\"8\" cellspacing=\"0\" width=\"90%\" border=\"1\" style=\"border-collapse: collapse; width: 90%; border-style: solid;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center; width: 50%;\"\u003e\u003cstrong\u003eItem\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center; width: 50%;\"\u003e\u003cstrong\u003eValue\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003ePenicillin\/Streptomycin\/Neomycin, Sterile\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eTotal Product Size\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIndividual Container Size\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e10ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eNumber of Containers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eQuantity\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterile\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eYes\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2 to 8°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eRefrigeration Requirements\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRefrigerator\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShipping Conditions\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eIce\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDangerous Goods\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eNo\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUNSPSC Code\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e51101500\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eUNSPSC Category\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAntibiotics and Antimycotics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStock Status\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eUsually in Stock\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eDocuments\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/COA.BS734-NO_LOT.pdf?v=1734080627\" target=\"_blank\"\u003e\u003cstrong\u003eCOA\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca type=\"application\/pdf\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/MSDS.BS734-Pen_Strep_Neomycin.pdf?v=1734080645\" target=\"_blank\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Usage Note\u003c\/h2\u003e\n\u003cp\u003eFor research use only. Not intended for human or veterinary therapeutic use. Handle antibiotics in accordance with institutional biosafety guidelines.\u003c\/p\u003e","brand":"BioBasic","offers":[{"title":"10mL","offer_id":44907632558261,"sku":"BS734-10mL","price":14.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/5_51171570-2f42-4dea-ad51-662cbf5809a0.jpg?v=1765847870"},{"product_id":"mycoplasma-detection-kit","title":"Mycoplasma Detection Kit","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eMycoplasma contamination is a common problem in cell culture. It can alter cell characteristics. For example, some mycoplasma species rapidly consume arginine in the culture medium, competing with cells for nutrients, which slows or halts cell growth. Mycoplasma can also change cellular enzyme profiles, membrane composition, and induce chromosomal abnormalities or pathological changes. These issues can seriously compromise experimental results and lead to unreliable conclusions.\u003c\/p\u003e\n\u003cp\u003eMycoplasma detection methods include culture, fluorescent staining, metabolite detection, and PCR.\u003c\/p\u003e\n\u003cp\u003eCulture methods require more than three weeks and cannot detect mycoplasma species that do not form colonies. Fluorescent staining has low sensitivity and requires an expensive fluorescence microscope. Metabolite detection also suffers from low sensitivity, and serum used in complete culture media—although filtered to remove mycoplasma—may still retain metabolites that cause false positives, in addition to being costly. These methods are not well-suited for routine mycoplasma monitoring in laboratories.\u003c\/p\u003e\n\u003cp\u003ePCR-based detection requires only a standard PCR thermal cycler and electrophoresis equipment, is technically simple, time-efficient, and cost-effective, making it ideal for routine monitoring of mycoplasma contamination in cell culture.\u003c\/p\u003e\n\u003cp\u003eThis product uses PCR-based detection. Primers are designed based on conserved sequences in mycoplasma genomes, and amplification products are analyzed by gel electrophoresis. The kit detects more than 40 mycoplasma species, including the eight species most commonly encountered in cell culture.\u003c\/p\u003e\n\u003cp\u003eThe limit of detection is as low as 5 copies, with an accuracy of 99%. Results are available within 3 hours. A positive result yields a 280 bp band. An internal control is included in the kit to monitor for PCR-related interference; the internal control band appears at 70 bp.\u003c\/p\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"width: 90%; border-collapse: collapse;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eComponent\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eVolume\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eMycoScan PCR MasterMix 2X\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e625μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003eMycoScan Positive Control 1X\u003c\/td\u003e\n\u003ctd style=\"width: 50%; text-align: center; vertical-align: middle;\"\u003e25μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eOperating Instructions\u003c\/h2\u003e\n\u003ch3\u003eSample Preparation\u003c\/h3\u003e\n\u003cp\u003eAfter culturing cells for 2 days (3 days yields better results), collect 1 mL of culture supernatant and centrifuge at 3000 rpm for 5 minutes to pellet cell debris.\u003c\/p\u003e\n\u003cp\u003eTransfer 500 μL of the supernatant to a fresh 1.5 mL microcentrifuge tube.\u003c\/p\u003e\n\u003cp\u003ePlace the tube in a boiling water bath (100 °C) for 10 minutes to lyse mycoplasma and release DNA.\u003c\/p\u003e\n\u003cp\u003eAllow the sample to cool to room temperature before proceeding to the next step.\u003c\/p\u003e\n\u003ch3\u003ePCR Reaction\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e1. Reaction Setup\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eComponent\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eVolume\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e2× PCR Mix\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12.5 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample lysate from Step 1 \/ Positive control \/ Negative control\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e2.5 μl (sample) \/ 1 μl (control)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSterile water\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTo 25 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eNote: Positive and negative controls must be included in each experiment.\u003c\/p\u003e\n\u003cp\u003eThe positive control is provided with the kit. Fresh basal culture medium or sterile water is recommended as the negative control.\u003c\/p\u003e\n\u003cp\u003eAdd 1 μL of the positive control.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Thermal Cycling Conditions\u003c\/strong\u003e\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTemperature\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eTime\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003eCycles\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e2 min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e94°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e30 sec\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\" rowspan=\"3\"\u003e35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e55°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e15 sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e30 sec\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e72°C\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e5 min\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center; vertical-align: middle;\"\u003e1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Electrophoresis Detection\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePerform electrophoresis using a 1.5–2% agarose gel.\u003c\/p\u003e\n\u003cp\u003eMycoplasma-positive result: Both a 280 bp band and a 70 bp band (the latter may be faint) are present.\u003c\/p\u003e\n\u003cp\u003eMycoplasma-negative result: Only a faint 70 bp band is present.\u003c\/p\u003e\n\u003ch2\u003ePrecautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eSamples should be culture supernatant collected from cells grown for 2–3 days to allow trace amounts of mycoplasma to be amplified during culture.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTo avoid false-positive or false-negative results, always include positive and negative controls in each test.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eTo ensure detection efficiency and avoid interference from cell genomic DNA, perform sample preparation (boiling) before PCR amplification.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eCollected culture supernatant can be stored briefly at 4 °C and tested within one week without affecting results. However, mycoplasma DNA released after boiling is unstable at low concentrations and prone to degradation, which may cause false negatives. Therefore, samples should be tested on the same day after sample preparation.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eNewly introduced cell lines should be tested for mycoplasma contamination to prevent cross-contamination of clean cultures. For routine monitoring, test in-culture cells once per month, supplemented with random weekly tests, to ensure early detection. For cells undergoing mycoplasma eradication treatment, test weekly until negative results are obtained and maintained for at least one month; thereafter, return to monthly testing with random weekly checks.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e1. What should I do if a faint, smeared band appears near 280 bp after electrophoresis?\u003c\/strong\u003e\u003cbr\u003eAnswer: It is recommended to collect fresh culture supernatant after 2–3 days of normal culture and repeat the test.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e2. Why do I see ladder-like bands not at 280 bp?\u003c\/strong\u003e\u003cbr\u003eAnswer: This is likely due to interference from cell genomic DNA. Perform sample preparation (boiling) again before amplification to eliminate genomic DNA interference.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e3. Why is there no internal control band?\u003c\/strong\u003e\u003cbr\u003eAnswer: The amount of dNTPs in the PCR system is fixed. When mycoplasma DNA in the reaction is excessive, the amplification efficiency of the internal control may decrease, and the internal control band may be absent after electrophoresis.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e4. I see black granular material under the microscope, but the PCR result is negative. Why?\u003c\/strong\u003e\u003cbr\u003eAnswer: There is no consensus on the identity of black granular material. It may be referred to as “black specks,” cell debris, inorganic particles, or fungi. Therefore, the presence of such material does not necessarily indicate mycoplasma contamination.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e5. Why do results from this PCR kit differ from those obtained with other detection kits?\u003c\/strong\u003e\u003cbr\u003eAnswer: Different kits are based on different detection principles. For example, some kits detect mycoplasma-specific metabolites. Although serum used in complete culture media is filtered to remove mycoplasma, residual metabolites may still cause false positives, leading to inconsistent results.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Detection_Kit_Instruction_Manual.pdf?v=1774935960\"\u003e\u003cstrong\u003eManual\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"PLEX","offers":[{"title":"50RXNS","offer_id":52401851498677,"sku":"BF-MD50","price":125.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Mycoplasma_Detection_Kit.jpg?v=1774936356"}],"url":"https:\/\/biofargo.com\/collections\/cell-culture-contamination-control.oembed","provider":"Biofargo","version":"1.0","type":"link"}